Standard name | |
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Human Ortholog | |
Description | 3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; restores resistance to glycolaldehyde by coupling reduction of glycolaldehyde to ethylene glycol and oxidation of NADPH to NADP+; protein abundance increases in response to DNA replication stress; methylglyoxal reductase (NADPH-dependent) is also known as D-lactaldehyde dehydrogenase |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 3 |
Bud | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 1 | 6 | 3 | 19 | 10 | 0 | 1 | 0 | 0 | 0 | 0 | 12 | 5 | 5 | 4 | 12 | 19 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 1 | 4 | 8 | 7 | 3 | 4 | 4 | 5 | 5 | 0 | 2 | 5 | 12 | 11 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 281 | 203 | 289 | 298 | 452 | 317 | 361 | 347 | 192 | 235 | 225 | 248 | 323 | 548 | 600 | 289 | 324 | 260 | 259 | 181 | 270 | 251 | 422 | 413 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 1 | 4 | 6 | 7 | 3 | 5 | 1 | 0 | 3 | 3 | 3 | 4 | 6 | 4 | 1 | 1 | 1 | 0 | 5 | 3 |
Endosome | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 0 | 1 | 1 | 3 | 0 | 1 | 1 | 2 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Mitochondria | 4 | 2 | 0 | 1 | 6 | 34 | 54 | 22 | 80 | 57 | 96 | 57 | 0 | 0 | 3 | 2 | 0 | 1 | 0 | 0 | 2 | 2 | 4 | 0 |
Nucleus | 2 | 1 | 0 | 2 | 4 | 4 | 5 | 14 | 2 | 9 | 10 | 9 | 2 | 9 | 3 | 1 | 1 | 2 | 1 | 3 | 2 | 0 | 2 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 3 | 5 | 8 |
Vac/Vac Membrane | 0 | 2 | 6 | 7 | 35 | 37 | 85 | 54 | 34 | 68 | 45 | 48 | 11 | 22 | 17 | 25 | 46 | 17 | 6 | 4 | 1 | 8 | 9 | 7 |
Unique Cell Count | 285 | 208 | 295 | 305 | 485 | 376 | 466 | 430 | 278 | 333 | 344 | 353 | 334 | 568 | 621 | 312 | 355 | 279 | 293 | 203 | 292 | 282 | 478 | 475 |
Labelled Cell Count | 287 | 212 | 299 | 309 | 504 | 406 | 520 | 450 | 322 | 386 | 403 | 380 | 340 | 587 | 633 | 334 | 390 | 290 | 293 | 203 | 292 | 282 | 478 | 475 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.3 | 10.4 | 8.1 | 8.1 | 8.3 | 7.1 | 7.2 | 7.9 | 6.9 | 7.8 | 7.2 | 8.2 | 12.9 | 13.0 | 13.2 | 19.1 | 21.1 | 20.2 | 7.8 | 8.1 | 8.3 |
Std Deviation (1e-4) | 2.5 | 3.7 | 2.1 | 2.5 | 2.3 | 1.9 | 2.0 | 2.3 | 2.5 | 2.9 | 3.7 | 3.6 | 5.8 | 5.4 | 6.0 | 7.6 | 11.1 | 8.0 | 2.2 | 2.5 | 2.3 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.03 | -0.18 | -0.17 | -0.04 | -0.24 | -0.06 | -0.17 | 0.01 | 0.67 | 0.68 | 0.7 | 1.23 | 1.38 | 1.32 | -0.06 | -0.01 | 0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.8 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 2.2 | 0 |
Cytoplasm | -0.2 | -3.0 | -5.9 | -7.8 | -7.0 | -9.4 | -9.2 | -10.3 | -9.3 | -1.0 | -1.2 | -1.1 | -3.1 | -3.7 | -2.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.3 | 6.1 | 3.9 | 9.9 | 7.5 | 9.8 | 7.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.2 | 3.1 | 4.1 | 6.7 | 5.1 | 4.8 | 7.1 | 5.1 | 5.3 | 1.0 | 1.4 | 0.6 | 3.3 | 5.1 | 2.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.1332 | 9.1052 | 9.0664 | 8.3436 | 8.0112 | 8.7586 | 5.9889 | 7.8352 | 7.3037 | 7.0082 | 5.9269 | 7.8721 | 9.1817 | 12.4018 | 11.1871 | 11.9348 | 8.3251 | 10.8902 |
Actin | 0.0068 | 0.0012 | 0.0021 | 0.0019 | 0.0015 | 0.0015 | 0.0243 | 0.0052 | 0.003 | 0.001 | 0.0048 | 0.0011 | 0.0021 | 0.0019 | 0.0006 | 0.0003 | 0.0022 | 0.0006 |
Bud | 0.0018 | 0.0063 | 0.0026 | 0.0115 | 0.004 | 0.0008 | 0.0022 | 0.0037 | 0.0036 | 0.0008 | 0.0282 | 0.0003 | 0.0003 | 0.0032 | 0.0008 | 0.001 | 0.015 | 0.0015 |
Bud Neck | 0.002 | 0.0005 | 0.0011 | 0.0032 | 0.0014 | 0.0017 | 0.0088 | 0.0005 | 0.0005 | 0.001 | 0.0013 | 0.0019 | 0.0004 | 0.0004 | 0.0005 | 0.0005 | 0.0008 | 0.0015 |
Bud Periphery | 0.0009 | 0.0027 | 0.0014 | 0.0053 | 0.001 | 0.0004 | 0.0019 | 0.0014 | 0.0016 | 0.0021 | 0.012 | 0.0002 | 0.0002 | 0.0012 | 0.0004 | 0.0004 | 0.0167 | 0.0012 |
Bud Site | 0.0049 | 0.0059 | 0.0026 | 0.0044 | 0.0012 | 0.0003 | 0.0049 | 0.0152 | 0.0021 | 0.0004 | 0.0027 | 0.0002 | 0.0011 | 0.0027 | 0.002 | 0.0003 | 0.0016 | 0.0002 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.8586 | 0.918 | 0.9159 | 0.8417 | 0.8357 | 0.8993 | 0.8101 | 0.9278 | 0.9122 | 0.8856 | 0.8252 | 0.9085 | 0.9479 | 0.9511 | 0.9603 | 0.9175 | 0.9229 | 0.9433 |
Cytoplasmic Foci | 0.0151 | 0.004 | 0.0065 | 0.0063 | 0.0175 | 0.0118 | 0.0118 | 0.0046 | 0.0058 | 0.004 | 0.0075 | 0.0085 | 0.0044 | 0.0047 | 0.0023 | 0.0029 | 0.0023 | 0.0028 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0054 | 0.002 | 0.0024 | 0.0031 | 0.0039 | 0.0034 | 0.0088 | 0.0015 | 0.0019 | 0.0032 | 0.0024 | 0.0025 | 0.0027 | 0.0018 | 0.0016 | 0.0011 | 0.0021 | 0.0024 |
Endosome | 0.0108 | 0.0024 | 0.0026 | 0.0028 | 0.0035 | 0.0059 | 0.0242 | 0.0012 | 0.0035 | 0.0011 | 0.0082 | 0.0041 | 0.003 | 0.001 | 0.001 | 0.0278 | 0.0013 | 0.0018 |
Golgi | 0.001 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0044 | 0.0003 | 0.0004 | 0.0001 | 0.007 | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0.0022 | 0.0001 | 0 |
Lipid Particles | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0024 | 0 | 0.0001 | 0.0001 | 0.0161 | 0.0001 | 0.0012 | 0 | 0 | 0.0006 | 0 | 0 |
Mitochondria | 0.0008 | 0.0005 | 0.0005 | 0.0014 | 0.0046 | 0.0009 | 0.0045 | 0.0003 | 0.0009 | 0.0008 | 0.0066 | 0.0004 | 0.0009 | 0.0003 | 0.0002 | 0.0121 | 0.0003 | 0.0002 |
None | 0.0292 | 0.0074 | 0.0096 | 0.0235 | 0.0106 | 0.0119 | 0.0454 | 0.0149 | 0.0437 | 0.0749 | 0.0442 | 0.0337 | 0.0083 | 0.0058 | 0.0051 | 0.0047 | 0.0038 | 0.0027 |
Nuclear Periphery | 0.0092 | 0.0041 | 0.0049 | 0.0074 | 0.0105 | 0.0059 | 0.0186 | 0.002 | 0.0024 | 0.0019 | 0.0032 | 0.0066 | 0.0039 | 0.0028 | 0.0029 | 0.0015 | 0.0034 | 0.0038 |
Nucleolus | 0.0004 | 0.0002 | 0.0001 | 0.0018 | 0.0022 | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0418 | 0.0387 | 0.0387 | 0.0732 | 0.0836 | 0.0469 | 0.014 | 0.0168 | 0.0149 | 0.0197 | 0.0205 | 0.0266 | 0.0186 | 0.0179 | 0.0198 | 0.01 | 0.0238 | 0.0347 |
Peroxisomes | 0.0012 | 0.0002 | 0.002 | 0.0004 | 0.0008 | 0.0002 | 0.0022 | 0.0017 | 0.0003 | 0.0002 | 0.0021 | 0.0003 | 0.0002 | 0.0021 | 0.0001 | 0.0002 | 0.0001 | 0 |
Punctate Nuclear | 0.0055 | 0.0021 | 0.0046 | 0.0074 | 0.0132 | 0.0061 | 0.0053 | 0.0014 | 0.0015 | 0.0009 | 0.0032 | 0.0023 | 0.0014 | 0.001 | 0.001 | 0.0002 | 0.0012 | 0.0012 |
Vacuole | 0.0032 | 0.003 | 0.0015 | 0.0034 | 0.0034 | 0.002 | 0.0038 | 0.0011 | 0.0011 | 0.0013 | 0.0027 | 0.0015 | 0.0019 | 0.0015 | 0.0012 | 0.01 | 0.0019 | 0.0016 |
Vacuole Periphery | 0.0005 | 0.0003 | 0.0003 | 0.0006 | 0.0005 | 0.0004 | 0.0012 | 0.0001 | 0.0003 | 0.0003 | 0.0011 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0064 | 0.0003 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 48.2538 | 42.1774 | 39.8331 | 44.6042 | 34.0622 | 56.8008 | 51.6108 | 46.4451 | 51.3903 | 39.5074 |
Translational Efficiency | 1.1326 | 1.3758 | 1.5938 | 1.2703 | 1.2614 | 1.2564 | 1.5011 | 1.7474 | 1.4771 | 1.5217 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1931 | 1028 | 1915 | 1872 | 1511 | 1489 | 2115 | 1369 | 3442 | 2517 | 4030 | 3241 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 717.29 | 894.72 | 938.38 | 1157.99 | 757.13 | 854.12 | 1173.52 | 1185.80 | 734.78 | 870.70 | 1061.78 | 1169.74 |
Standard Deviation | 110.66 | 180.81 | 167.47 | 289.48 | 126.02 | 143.42 | 275.14 | 310.86 | 119.30 | 160.99 | 258.54 | 299.01 |
Intensity Change Log 2 | — | 0.318880 | 0.387616 | 0.690994 | — | 0.173898 | 0.632229 | 0.647248 | — | 0.246251 | 0.518394 | 0.668696 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000120 | 0.000399 | 0.000196 | 0.000424 | 0.000125 | 0.000497 | 0.000523 | 0.001998 | 0.000122 | 0.000457 | 0.000367 | 0.001088 |
Bud Neck | 0.013303 | 0.007144 | 0.003711 | 0.005077 | 0.004363 | 0.007688 | 0.002412 | 0.005114 | 0.009379 | 0.007466 | 0.003029 | 0.005093 |
Bud Site | 0.006656 | 0.016521 | 0.007209 | 0.018827 | 0.005217 | 0.016627 | 0.013139 | 0.035223 | 0.006024 | 0.016584 | 0.010321 | 0.025752 |
Cell Periphery | 0.000165 | 0.000113 | 0.000100 | 0.000108 | 0.000147 | 0.000077 | 0.000102 | 0.000205 | 0.000157 | 0.000092 | 0.000101 | 0.000149 |
Cytoplasm | 0.576938* | 0.730416* | 0.554761* | 0.655054* | 0.692351* | 0.708735* | 0.760067* | 0.599154* | 0.627603* | 0.717590* | 0.662509* | 0.631442* |
Cytoplasmic Foci | 0.176703 | 0.100231 | 0.005867 | 0.010472 | 0.128734 | 0.096399 | 0.007169 | 0.023893 | 0.155645 | 0.097964 | 0.006550 | 0.016141 |
Eisosomes | 0.000120 | 0.000031 | 0.000019 | 0.000011 | 0.000060 | 0.000038 | 0.000009 | 0.000025 | 0.000093 | 0.000035 | 0.000014 | 0.000017 |
Endoplasmic Reticulum | 0.001964 | 0.000695 | 0.001695 | 0.000908 | 0.000978 | 0.000452 | 0.000476 | 0.001410 | 0.001531 | 0.000551 | 0.001056 | 0.001120 |
Endosome | 0.009358 | 0.008362 | 0.001367 | 0.006596 | 0.006968 | 0.007796 | 0.000859 | 0.014016 | 0.008309 | 0.008027 | 0.001100 | 0.009730 |
Golgi | 0.002291 | 0.003611 | 0.000079 | 0.005330 | 0.001486 | 0.002166 | 0.000757 | 0.018454 | 0.001938 | 0.002756 | 0.000435 | 0.010873 |
Lipid Particles | 0.006244 | 0.001654 | 0.000694 | 0.000623 | 0.002875 | 0.001190 | 0.000384 | 0.002152 | 0.004765 | 0.001379 | 0.000531 | 0.001269 |
Mitochondria | 0.002588 | 0.002951 | 0.000529 | 0.002380 | 0.002927 | 0.001799 | 0.000350 | 0.007766 | 0.002737 | 0.002269 | 0.000435 | 0.004655 |
Mitotic Spindle | 0.000362 | 0.007917 | 0.006750 | 0.052961 | 0.003619 | 0.007340 | 0.015993 | 0.090307 | 0.001792 | 0.007575 | 0.011601 | 0.068736 |
None | 0.007965 | 0.005948 | 0.008493 | 0.010369 | 0.006678 | 0.002681 | 0.008591 | 0.009053 | 0.007400 | 0.004016 | 0.008544 | 0.009813 |
Nuclear Periphery | 0.000611 | 0.000190 | 0.001308 | 0.000896 | 0.000289 | 0.000194 | 0.000459 | 0.000877 | 0.000470 | 0.000192 | 0.000862 | 0.000888 |
Nuclear Periphery Foci | 0.000653 | 0.001588 | 0.001319 | 0.001676 | 0.001633 | 0.001536 | 0.001236 | 0.002500 | 0.001083 | 0.001558 | 0.001275 | 0.002024 |
Nucleolus | 0.001367 | 0.000590 | 0.000276 | 0.000417 | 0.000858 | 0.000938 | 0.000122 | 0.000419 | 0.001143 | 0.000796 | 0.000195 | 0.000418 |
Nucleus | 0.130600* | 0.056242 | 0.361599* | 0.160510* | 0.099328 | 0.057667 | 0.143883* | 0.109324 | 0.116872 | 0.057085 | 0.247338* | 0.138889* |
Peroxisomes | 0.002163 | 0.003015 | 0.000352 | 0.000382 | 0.001424 | 0.003446 | 0.000204 | 0.002680 | 0.001839 | 0.003270 | 0.000274 | 0.001353 |
Vacuole | 0.058399 | 0.051166 | 0.043520 | 0.062365 | 0.039257 | 0.081875 | 0.043107 | 0.067207 | 0.049996 | 0.069333 | 0.043303 | 0.064411 |
Vacuole Periphery | 0.001430 | 0.001216 | 0.000156 | 0.004612 | 0.000684 | 0.000858 | 0.000159 | 0.008223 | 0.001103 | 0.001004 | 0.000158 | 0.006138 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.39 | -5.63 | -5.62 | 1.02 | -2.50 | -2.22 | -2.35 | -3.07 | -1.25 | -1.95 | -3.35 | -3.41 | -4.25 | -0.66 | -2.25 |
Bud Neck | 5.47 | 9.25 | 10.37 | 3.94 | 0.55 | -3.88 | 3.76 | 2.86 | 6.43 | -1.45 | 2.91 | 10.38 | 10.52 | 7.09 | -0.55 |
Bud Site | -5.07 | 0.97 | -1.93 | 3.71 | -2.51 | -5.84 | -1.99 | -4.82 | 1.13 | -3.07 | -7.56 | -0.78 | -4.89 | 3.56 | -3.74 |
Cell Periphery | 4.25 | 7.79 | 8.44 | 1.25 | 0.15 | 4.66 | 3.04 | 1.83 | -3.94 | -1.59 | 7.42 | 6.52 | 5.74 | -2.35 | -1.25 |
Cytoplasm | -14.24 | 12.75 | 2.09 | 16.18 | -10.93 | -0.52 | 5.30 | 13.60 | 13.40 | 9.77 | -11.64 | 10.55 | 10.41 | 21.04 | 0.23 |
Cytoplasmic Foci | 12.01 | 40.16 | 39.47 | 19.82 | -3.76 | 6.42 | 29.64 | 28.17 | 23.52 | -3.89 | 14.29 | 49.35 | 48.09 | 31.20 | -4.74 |
Eisosomes | 17.16 | 19.46 | 21.68 | 7.47 | 7.87 | 4.92 | 12.00 | 7.84 | 4.16 | -2.64 | 16.18 | 22.99 | 21.65 | 7.86 | 0.35 |
Endoplasmic Reticulum | 4.41 | -1.67 | 4.21 | -0.76 | 12.94 | 2.61 | 2.01 | 1.73 | -2.05 | -0.48 | 5.36 | 0.29 | 4.50 | -2.64 | 10.26 |
Endosome | 1.38 | 13.94 | 11.49 | 7.88 | -0.72 | -0.52 | 11.79 | 5.69 | 5.99 | -3.42 | 1.08 | 18.33 | 12.44 | 10.25 | -3.20 |
Golgi | -1.30 | 9.39 | 3.11 | 2.73 | -2.91 | -0.80 | 2.39 | -1.44 | -0.94 | -2.77 | -1.26 | 6.84 | 0.17 | 1.15 | -3.67 |
Lipid Particles | 9.29 | 12.12 | 11.76 | 2.61 | -2.61 | 4.26 | 6.04 | 4.27 | -0.16 | -2.52 | 10.34 | 13.45 | 12.06 | 1.45 | -3.58 |
Mitochondria | -0.17 | 7.80 | 6.08 | 1.92 | -1.81 | 1.13 | 2.63 | 1.29 | 0.25 | -2.23 | 0.91 | 5.04 | 3.20 | 1.81 | -2.84 |
Mitotic Spindle | -4.24 | -6.44 | -11.08 | -6.62 | -7.91 | -2.20 | -6.33 | -8.16 | -6.78 | -4.40 | -4.90 | -9.04 | -13.56 | -9.64 | -8.19 |
None | 2.11 | 0.39 | -1.32 | -4.83 | -2.99 | 4.44 | -1.25 | -2.65 | -13.42 | -2.82 | 5.13 | -0.47 | -2.68 | -13.01 | -4.12 |
Nuclear Periphery | 1.72 | -5.70 | -2.64 | -19.87 | 10.30 | 1.48 | -11.70 | -10.15 | -12.30 | -1.03 | 1.94 | -6.93 | -4.77 | -22.31 | 6.22 |
Nuclear Periphery Foci | -4.93 | -6.02 | -5.79 | 1.61 | 1.01 | 0.22 | 0.01 | 0.38 | -0.06 | 0.44 | -1.43 | -4.51 | -2.81 | 0.43 | 1.15 |
Nucleolus | 2.55 | 3.43 | 3.26 | 2.44 | -0.53 | -0.08 | 3.63 | 3.20 | 1.51 | -3.67 | 1.26 | 4.69 | 4.27 | 1.79 | -1.60 |
Nucleus | 16.67 | -41.32 | -14.62 | -30.51 | 29.07 | 10.66 | -19.31 | -12.25 | -21.52 | 4.56 | 20.06 | -39.98 | -19.07 | -37.77 | 22.46 |
Peroxisomes | -1.51 | 8.61 | 8.84 | 5.73 | 0.11 | -2.54 | 4.48 | 2.69 | 4.04 | -1.92 | -2.73 | 9.42 | 7.82 | 6.37 | -1.54 |
Vacuole | 0.09 | -5.91 | -20.32 | -18.74 | -14.66 | -13.52 | -18.35 | -22.93 | -12.96 | -9.92 | -10.01 | -16.95 | -30.48 | -20.84 | -16.59 |
Vacuole Periphery | 1.23 | 8.46 | 1.48 | 0.64 | -2.67 | -0.85 | 6.10 | -1.37 | -1.14 | -2.56 | 1.24 | 10.26 | -0.05 | -0.61 | -3.68 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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