Standard name | |
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Human Ortholog | |
Description | Alpha 1,3 glucosyltransferase; involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; C998T transition in human ortholog ALG6 causes carbohydrate-deficient glycoprotein syndrome type-Ic; wild-type human ortholog ALG6 can partially complement yeast alg6 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 3 | 13 | 1 | 2 | 3 | 4 | 10 | 7 | 10 | 5 | 5 | 11 | 12 | 6 | 16 | 17 | 12 | 5 | 7 | 14 | 7 | 8 | 9 |
Bud | 2 | 0 | 3 | 4 | 1 | 5 | 9 | 5 | 6 | 12 | 0 | 11 | 0 | 1 | 0 | 4 | 0 | 2 | 3 | 12 | 10 | 4 | 4 | 16 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 0 | 1 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 1 | 7 | 8 | 5 | 2 | 6 | 12 | 13 | 6 | 11 | 13 | 25 | 25 | 18 | 16 | 15 | 2 | 0 | 2 | 0 | 0 | 0 |
Cytoplasm | 103 | 98 | 115 | 81 | 69 | 72 | 95 | 115 | 87 | 98 | 35 | 59 | 120 | 152 | 173 | 87 | 48 | 32 | 50 | 35 | 47 | 44 | 39 | 41 |
Endoplasmic Reticulum | 24 | 107 | 128 | 66 | 36 | 33 | 96 | 66 | 68 | 131 | 34 | 37 | 140 | 147 | 143 | 12 | 3 | 4 | 172 | 166 | 166 | 125 | 105 | 103 |
Endosome | 15 | 8 | 0 | 3 | 9 | 6 | 4 | 5 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 22 | 6 | 3 | 4 | 6 | 3 | 5 | 4 | 1 |
Golgi | 0 | 3 | 1 | 2 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 2 | 13 | 1 | 5 | 2 | 5 | 4 | 0 | 1 |
Mitochondria | 46 | 7 | 36 | 47 | 49 | 138 | 188 | 149 | 191 | 243 | 108 | 124 | 9 | 6 | 4 | 26 | 15 | 14 | 27 | 25 | 51 | 20 | 23 | 9 |
Nucleus | 1 | 0 | 1 | 0 | 0 | 2 | 2 | 4 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 6 | 7 | 14 | 5 | 5 | 4 | 16 | 7 | 9 | 21 | 4 | 8 | 9 | 12 | 8 | 1 | 0 | 0 | 6 | 12 | 9 | 3 | 1 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 3 | 3 | 0 | 0 | 1 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 1 | 2 | 0 | 1 | 1 | 0 | 1 | 1 | 4 | 3 | 2 | 3 | 3 | 5 | 1 | 2 | 2 |
Vac/Vac Membrane | 79 | 54 | 21 | 13 | 27 | 44 | 36 | 40 | 20 | 31 | 11 | 18 | 8 | 18 | 21 | 49 | 64 | 50 | 6 | 12 | 9 | 5 | 8 | 12 |
Unique Cell Count | 237 | 253 | 286 | 201 | 175 | 236 | 350 | 325 | 308 | 415 | 158 | 203 | 275 | 334 | 349 | 214 | 171 | 109 | 296 | 293 | 337 | 224 | 209 | 211 |
Labelled Cell Count | 279 | 288 | 334 | 229 | 209 | 312 | 458 | 412 | 403 | 565 | 205 | 275 | 313 | 379 | 387 | 247 | 190 | 138 | 296 | 293 | 337 | 224 | 209 | 211 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.0 | 7.2 | 5.8 | 5.5 | 6.2 | 5.2 | 4.8 | 5.0 | 4.9 | 5.0 | 4.8 | 5.0 | 6.9 | 6.7 | 6.6 | 7.2 | 8.7 | 8.7 | 6.4 | 6.6 | 7.5 |
Std Deviation (1e-4) | 2.4 | 2.5 | 2.0 | 2.0 | 2.2 | 1.9 | 1.7 | 1.9 | 1.9 | 1.7 | 1.7 | 1.8 | 2.4 | 2.4 | 2.3 | 1.3 | 1.5 | 1.3 | 2.1 | 2.4 | 2.5 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.09 | -0.16 | -0.28 | -0.22 | -0.25 | -0.22 | -0.28 | -0.21 | 0.25 | 0.2 | 0.18 | 0.3 | 0.57 | 0.58 | 0.13 | 0.18 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.6 | -2.0 | -2.2 | 0 | -1.0 | -1.5 | -1.6 | 0 | -1.2 | -0.3 | -0.6 | -2.1 | 1.4 | 2.3 | 2.4 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 3.0 | 2.6 | 0 | 0 | 3.3 | 4.4 | 4.3 | 4.7 | 4.9 | 0 |
Cytoplasm | 0 | -0.2 | -2.3 | -3.5 | -1.2 | -3.1 | -4.7 | -3.9 | -2.5 | 0.8 | 1.3 | 2.4 | 0.1 | -2.6 | -2.0 |
Endoplasmic Reticulum | -2.6 | -5.3 | -7.6 | 0 | -6.5 | -5.9 | -3.6 | 0 | -6.1 | 1.5 | -0.2 | -1.0 | -9.6 | -9.8 | -7.7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.5 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.4 | 0 |
Mitochondria | 3.1 | 4.1 | 11.1 | 0 | 8.9 | 12.4 | 12.2 | 0 | 11.3 | -4.1 | -5.3 | -5.9 | -0.1 | -1.3 | 0.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.4 | -1.1 | -2.0 | 0 | -1.9 | -1.2 | 0.1 | 0 | -0.5 | -1.0 | -0.8 | -1.8 | -2.9 | -2.9 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.4 | 2.8 | 3.9 | 1.3 | 2.0 | -0.4 | 0.1 | -0.1 | 0.6 | -2.4 | -1.0 | -0.7 | 5.0 | 8.0 | 8.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.6713 | 3.0632 | 2.9687 | 2.5925 | 1.8509 | 2.7316 | 3.8059 | 2.3786 | 2.4339 | 1.8263 | 2.0302 | 2.2518 | 3.3209 | 3.4077 | 3.3706 | 3.713 | 3.0442 | 3.4012 |
Actin | 0.0202 | 0.0055 | 0.0041 | 0.0006 | 0.0006 | 0.002 | 0.107 | 0.007 | 0.0209 | 0.0057 | 0.0037 | 0.018 | 0.001 | 0.001 | 0.0037 | 0.0014 | 0.0003 | 0.0024 |
Bud | 0.0002 | 0.0008 | 0.0004 | 0.0001 | 0.0032 | 0.0001 | 0.0019 | 0.0006 | 0.0005 | 0.0001 | 0.0001 | 0.0011 | 0.0002 | 0.0014 | 0.0006 | 0.0002 | 0 | 0.0009 |
Bud Neck | 0.0013 | 0.0003 | 0.0039 | 0.0018 | 0.0001 | 0.0023 | 0.0052 | 0.0004 | 0.007 | 0.0004 | 0.0002 | 0.0009 | 0.0002 | 0.0009 | 0.0004 | 0.0012 | 0.0003 | 0.001 |
Bud Periphery | 0.0003 | 0.0012 | 0.001 | 0.0002 | 0.0098 | 0.0004 | 0.0065 | 0.003 | 0.0009 | 0.0002 | 0.0002 | 0.0052 | 0.0003 | 0.0018 | 0.0016 | 0.0009 | 0.0001 | 0.0012 |
Bud Site | 0.0013 | 0.0023 | 0.0013 | 0.0005 | 0.0002 | 0.0006 | 0.0211 | 0.0053 | 0.0087 | 0.0003 | 0.0003 | 0.0004 | 0.0043 | 0.0088 | 0.002 | 0.0001 | 0.0002 | 0.0002 |
Cell Periphery | 0.0006 | 0.0011 | 0.0008 | 0.0003 | 0.0006 | 0.0006 | 0.0023 | 0.0013 | 0.001 | 0.0007 | 0.0002 | 0.0006 | 0.0008 | 0.0009 | 0.0011 | 0.0006 | 0.0002 | 0.0005 |
Cytoplasm | 0.1803 | 0.2652 | 0.1577 | 0.1216 | 0.1728 | 0.2094 | 0.0721 | 0.1234 | 0.1198 | 0.0709 | 0.1354 | 0.1728 | 0.107 | 0.1572 | 0.1639 | 0.1463 | 0.1046 | 0.1829 |
Cytoplasmic Foci | 0.0254 | 0.0225 | 0.0111 | 0.0158 | 0.0059 | 0.0155 | 0.0478 | 0.0249 | 0.0275 | 0.006 | 0.0214 | 0.0093 | 0.009 | 0.0092 | 0.0105 | 0.0061 | 0.0042 | 0.0109 |
Eisosomes | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0016 | 0.0005 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0005 | 0.0003 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.2545 | 0.1971 | 0.2847 | 0.2795 | 0.0623 | 0.2564 | 0.2553 | 0.2111 | 0.2485 | 0.3066 | 0.0651 | 0.1838 | 0.3006 | 0.234 | 0.2518 | 0.2277 | 0.2082 | 0.2184 |
Endosome | 0.0198 | 0.0204 | 0.0148 | 0.0217 | 0.0026 | 0.0161 | 0.0656 | 0.0327 | 0.0296 | 0.015 | 0.0336 | 0.0163 | 0.0152 | 0.031 | 0.0189 | 0.0177 | 0.0058 | 0.0158 |
Golgi | 0.0043 | 0.0019 | 0.0015 | 0.0008 | 0.0003 | 0.002 | 0.0259 | 0.0039 | 0.0107 | 0.0012 | 0.0174 | 0.0088 | 0.0006 | 0.0013 | 0.0014 | 0.0008 | 0.0002 | 0.0008 |
Lipid Particles | 0.0141 | 0.0105 | 0.0043 | 0.0076 | 0.0008 | 0.0076 | 0.0216 | 0.0121 | 0.0247 | 0.01 | 0.0387 | 0.0031 | 0.0079 | 0.01 | 0.0096 | 0.0047 | 0.0011 | 0.0049 |
Mitochondria | 0.0018 | 0.0007 | 0.0009 | 0.0007 | 0.0009 | 0.0081 | 0.0116 | 0.006 | 0.0043 | 0.0016 | 0.0191 | 0.0052 | 0.0007 | 0.0031 | 0.0028 | 0.0017 | 0.0003 | 0.0014 |
None | 0.3871 | 0.4375 | 0.4597 | 0.4936 | 0.6722 | 0.4389 | 0.2331 | 0.5314 | 0.4458 | 0.5596 | 0.5752 | 0.5301 | 0.499 | 0.4788 | 0.4573 | 0.5111 | 0.6373 | 0.4999 |
Nuclear Periphery | 0.0534 | 0.0193 | 0.0338 | 0.0369 | 0.0491 | 0.0226 | 0.0626 | 0.0148 | 0.014 | 0.0138 | 0.0066 | 0.0278 | 0.0398 | 0.0336 | 0.0507 | 0.051 | 0.0283 | 0.0371 |
Nucleolus | 0.0005 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0007 | 0.0017 | 0.0007 | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0008 | 0.0006 | 0.0005 | 0.0002 | 0.0004 |
Nucleus | 0.0087 | 0.0045 | 0.0048 | 0.0069 | 0.0139 | 0.0051 | 0.0092 | 0.0026 | 0.0027 | 0.0016 | 0.0011 | 0.0061 | 0.0041 | 0.0107 | 0.0079 | 0.0157 | 0.0029 | 0.0065 |
Peroxisomes | 0.015 | 0.0003 | 0.0008 | 0.0007 | 0.0002 | 0.001 | 0.0135 | 0.0005 | 0.0185 | 0.0003 | 0.0757 | 0.0012 | 0.0002 | 0.0004 | 0.0005 | 0.0004 | 0.0001 | 0.0004 |
Punctate Nuclear | 0.0034 | 0.0024 | 0.0027 | 0.0014 | 0.0006 | 0.0034 | 0.014 | 0.0027 | 0.0079 | 0.0012 | 0.0015 | 0.0038 | 0.0019 | 0.0027 | 0.0041 | 0.0034 | 0.0021 | 0.0062 |
Vacuole | 0.0051 | 0.0054 | 0.0091 | 0.0072 | 0.0027 | 0.0056 | 0.0145 | 0.014 | 0.0046 | 0.0033 | 0.003 | 0.0037 | 0.0051 | 0.0101 | 0.0075 | 0.006 | 0.003 | 0.0061 |
Vacuole Periphery | 0.002 | 0.0007 | 0.002 | 0.0017 | 0.0008 | 0.0013 | 0.0061 | 0.0012 | 0.0014 | 0.0009 | 0.0013 | 0.0012 | 0.0014 | 0.0021 | 0.0028 | 0.0023 | 0.0006 | 0.0018 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 43.8662 | 32.5776 | 37.6039 | 48.7774 | 35.8747 | 34.3991 | 47.5788 | 46.7277 | 41.4763 | 54.8234 |
Translational Efficiency | 0.5696 | 0.6062 | 0.59 | 0.4397 | 0.52 | 0.7973 | 0.5018 | 0.5388 | 0.6454 | 0.4956 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1089 | 1459 | 493 | 1510 | 840 | 1317 | 204 | 73 | 1929 | 2776 | 697 | 1583 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 688.50 | 841.94 | 1047.63 | 916.32 | 749.00 | 851.68 | 1105.79 | 979.28 | 714.85 | 846.56 | 1064.65 | 919.22 |
Standard Deviation | 116.39 | 182.24 | 189.20 | 165.30 | 128.58 | 175.46 | 182.86 | 182.97 | 125.48 | 179.12 | 189.22 | 166.68 |
Intensity Change Log 2 | — | 0.290261 | 0.605601 | 0.412395 | — | 0.185346 | 0.562040 | 0.386756 | — | 0.236548 | 0.583068 | 0.399093 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001070 | 0.002345 | 0.002147 | 0.004633 | 0.001381 | 0.003032 | 0.000985 | 0.004702 | 0.001205 | 0.002671 | 0.001807 | 0.004636 |
Bud Neck | 0.010438 | 0.008483 | 0.001908 | 0.012444 | 0.003467 | 0.011136 | 0.002283 | 0.005575 | 0.007402 | 0.009742 | 0.002018 | 0.012127 |
Bud Site | 0.007548 | 0.014380 | 0.002448 | 0.041021 | 0.006473 | 0.013290 | 0.004865 | 0.016097 | 0.007080 | 0.013863 | 0.003155 | 0.039872 |
Cell Periphery | 0.001961 | 0.000616 | 0.000281 | 0.000877 | 0.000809 | 0.000962 | 0.000439 | 0.000467 | 0.001459 | 0.000780 | 0.000327 | 0.000858 |
Cytoplasm | 0.290214* | 0.204974* | 0.087054 | 0.250818* | 0.257670* | 0.213008* | 0.078460 | 0.244197* | 0.276042* | 0.208785* | 0.084539 | 0.250513* |
Cytoplasmic Foci | 0.124817 | 0.083227 | 0.022385 | 0.019378 | 0.105981 | 0.067152 | 0.014051 | 0.067642 | 0.116614 | 0.075600 | 0.019946 | 0.021604 |
Eisosomes | 0.000539 | 0.000150 | 0.000077 | 0.000313 | 0.000244 | 0.000201 | 0.000053 | 0.000172 | 0.000411 | 0.000174 | 0.000070 | 0.000306 |
Endoplasmic Reticulum | 0.328500* | 0.441472* | 0.190054* | 0.309548* | 0.359922* | 0.426745* | 0.581334* | 0.172987* | 0.342183* | 0.434485* | 0.304575* | 0.303250* |
Endosome | 0.013154 | 0.026497 | 0.017793 | 0.012806 | 0.018637 | 0.022540 | 0.012371 | 0.026202 | 0.015542 | 0.024620 | 0.016206 | 0.013424 |
Golgi | 0.012611 | 0.014618 | 0.000754 | 0.003113 | 0.009727 | 0.009474 | 0.000468 | 0.003374 | 0.011355 | 0.012178 | 0.000670 | 0.003126 |
Lipid Particles | 0.067300 | 0.034087 | 0.029445 | 0.013529 | 0.053435 | 0.040344 | 0.013178 | 0.044419 | 0.061263 | 0.037056 | 0.024684 | 0.014953 |
Mitochondria | 0.014260 | 0.017984 | 0.001369 | 0.004724 | 0.012459 | 0.030801 | 0.000927 | 0.000471 | 0.013476 | 0.024064 | 0.001240 | 0.004528 |
Mitotic Spindle | 0.000419 | 0.005783 | 0.005962 | 0.018489 | 0.001490 | 0.006905 | 0.003444 | 0.040392 | 0.000885 | 0.006316 | 0.005225 | 0.019499 |
None | 0.014564 | 0.007478 | 0.002237 | 0.002558 | 0.006249 | 0.012467 | 0.001661 | 0.002023 | 0.010943 | 0.009845 | 0.002069 | 0.002533 |
Nuclear Periphery | 0.006416 | 0.010062 | 0.234594* | 0.012092 | 0.012730 | 0.021549 | 0.043798 | 0.009787 | 0.009166 | 0.015511 | 0.178751* | 0.011985 |
Nuclear Periphery Foci | 0.012697 | 0.030790 | 0.202317* | 0.041253 | 0.025552 | 0.032390 | 0.094974 | 0.061614 | 0.018295 | 0.031549 | 0.170900 | 0.042192 |
Nucleolus | 0.001397 | 0.001240 | 0.002772 | 0.000437 | 0.001088 | 0.001432 | 0.000190 | 0.000734 | 0.001262 | 0.001331 | 0.002016 | 0.000450 |
Nucleus | 0.056920 | 0.040219 | 0.144448* | 0.134052* | 0.080072 | 0.040195 | 0.080697 | 0.112981 | 0.067002 | 0.040208 | 0.125789 | 0.133081* |
Peroxisomes | 0.007949 | 0.005462 | 0.001266 | 0.001658 | 0.005748 | 0.005849 | 0.000657 | 0.006002 | 0.006991 | 0.005646 | 0.001088 | 0.001858 |
Vacuole | 0.024337 | 0.044275 | 0.049518 | 0.113439 | 0.033165 | 0.036205 | 0.064908 | 0.179389 | 0.028181 | 0.040446 | 0.054022 | 0.116480 |
Vacuole Periphery | 0.002892 | 0.005859 | 0.001172 | 0.002820 | 0.003700 | 0.004322 | 0.000259 | 0.000774 | 0.003244 | 0.005130 | 0.000905 | 0.002726 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.13 | -0.77 | -4.09 | -1.66 | -1.09 | -1.86 | 0.45 | -0.74 | -0.01 | -0.84 | -2.80 | -0.64 | -3.93 | -1.36 | -1.65 |
Bud Neck | 1.07 | 6.07 | -0.97 | -2.59 | -10.34 | -6.03 | 0.19 | -0.93 | 3.27 | -0.98 | -2.31 | 5.91 | -3.61 | -1.57 | -9.98 |
Bud Site | -3.83 | 3.69 | -8.16 | -5.73 | -10.45 | -3.46 | -0.21 | -0.90 | 0.21 | -0.78 | -5.12 | 2.74 | -8.66 | -6.07 | -9.88 |
Cell Periphery | 4.20 | 5.23 | 3.91 | -0.52 | -3.86 | -0.41 | 1.71 | 2.03 | 1.82 | 0.55 | 2.69 | 5.54 | 3.67 | 0.71 | -3.42 |
Cytoplasm | 7.31 | 16.73 | 2.85 | -5.21 | -15.98 | 3.62 | 9.57 | 0.57 | -0.85 | -4.13 | 7.91 | 19.27 | 2.00 | -5.64 | -17.13 |
Cytoplasmic Foci | 6.08 | 16.56 | 19.37 | 15.89 | 1.78 | 5.86 | 13.55 | 3.52 | 0.24 | -4.32 | 8.56 | 21.10 | 23.66 | 18.36 | 0.30 |
Eisosomes | 4.18 | 5.01 | 2.74 | -1.31 | -2.41 | 0.73 | 6.29 | 2.25 | 0.53 | -4.51 | 3.99 | 6.27 | 2.21 | -0.92 | -2.56 |
Endoplasmic Reticulum | -6.55 | 8.92 | 8.78 | 17.09 | -1.04 | -3.80 | -3.76 | 7.50 | 9.85 | 8.83 | -7.44 | 4.63 | 11.92 | 20.70 | 5.04 |
Endosome | -9.02 | -2.76 | 3.00 | 11.67 | 4.61 | -1.84 | 3.29 | -0.11 | 0.52 | -1.31 | -7.36 | -0.53 | 4.66 | 12.38 | 4.14 |
Golgi | -1.03 | 10.07 | 8.25 | 8.49 | -2.04 | 0.13 | 7.55 | 4.84 | 5.29 | -1.59 | -0.63 | 12.50 | 9.39 | 10.05 | -2.33 |
Lipid Particles | 7.17 | 7.52 | 13.54 | 9.36 | 5.96 | 2.88 | 8.89 | 2.38 | 1.15 | -1.59 | 7.36 | 10.20 | 16.68 | 12.53 | 5.16 |
Mitochondria | -1.57 | 8.28 | 6.38 | 7.85 | -3.16 | -6.17 | 6.80 | 7.24 | 12.23 | 1.62 | -5.66 | 10.68 | 7.77 | 12.79 | -3.29 |
Mitotic Spindle | -4.66 | -2.83 | -7.65 | -4.68 | -3.85 | -3.73 | -1.63 | -1.92 | -1.48 | -1.61 | -5.97 | -3.12 | -7.65 | -4.91 | -4.53 |
None | 3.28 | 6.08 | 6.07 | 4.93 | -0.33 | -3.29 | 3.51 | 3.25 | 6.13 | 0.03 | 0.75 | 6.94 | 6.77 | 7.85 | -0.64 |
Nuclear Periphery | -2.35 | -17.02 | -2.75 | -0.27 | 16.72 | -3.46 | -3.46 | 1.83 | 4.42 | 4.05 | -4.37 | -16.32 | -0.86 | 3.60 | 16.20 |
Nuclear Periphery Foci | -6.58 | -16.82 | -6.34 | 0.28 | 15.03 | -1.81 | -5.06 | -1.37 | -0.85 | 2.31 | -5.83 | -16.75 | -4.54 | 0.38 | 15.01 |
Nucleolus | 0.26 | -2.29 | 2.12 | 4.60 | 4.60 | -1.12 | 4.47 | 1.46 | 2.41 | -1.74 | -0.27 | -1.94 | 2.98 | 5.86 | 4.32 |
Nucleus | 4.08 | -8.24 | -20.21 | -23.43 | -1.93 | 7.76 | -2.57 | -2.83 | -4.72 | -0.85 | 8.46 | -8.08 | -19.02 | -25.03 | -3.12 |
Peroxisomes | 2.33 | 7.51 | 7.64 | 6.87 | 0.06 | -0.12 | 6.26 | 0.81 | 0.87 | -1.29 | 1.76 | 9.49 | 8.81 | 8.69 | -0.81 |
Vacuole | -6.77 | -6.49 | -27.12 | -23.14 | -18.59 | -0.88 | -5.49 | -8.00 | -7.91 | -5.00 | -5.51 | -8.00 | -27.76 | -25.64 | -16.22 |
Vacuole Periphery | -4.14 | 4.56 | 1.92 | 5.32 | -1.34 | -0.85 | 5.88 | 4.98 | 7.12 | -1.01 | -3.69 | 6.95 | 2.80 | 5.82 | -1.90 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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