Standard name | |
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Human Ortholog | |
Description | Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; similar to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 1 | 2 | 13 | 8 | 12 | 13 | 7 | 19 | 1 | 0 | 0 | 4 | 3 | 4 | 1 | 0 | 0 | 0 | 1 | 3 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 2 | 3 | 7 | 1 | 0 | 0 | 0 | 1 | 0 | 4 | 0 | 0 | 3 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 0 | 2 | 16 | 25 | 21 | 34 | 13 | 19 | 44 | 15 | 38 | 8 | 9 | 6 | 46 | 32 | 32 | 0 | 0 | 0 | 1 | 0 | 2 |
Cytoplasm | 193 | 17 | 85 | 173 | 264 | 228 | 263 | 256 | 202 | 254 | 160 | 192 | 138 | 148 | 168 | 444 | 356 | 428 | 183 | 53 | 98 | 67 | 86 | 105 |
Endoplasmic Reticulum | 1 | 0 | 3 | 14 | 20 | 18 | 46 | 44 | 32 | 50 | 15 | 40 | 3 | 4 | 0 | 13 | 9 | 14 | 4 | 0 | 3 | 3 | 2 | 6 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
Golgi | 2 | 0 | 1 | 1 | 2 | 1 | 3 | 1 | 2 | 0 | 0 | 3 | 0 | 0 | 0 | 19 | 16 | 9 | 0 | 0 | 0 | 0 | 2 | 8 |
Mitochondria | 0 | 0 | 0 | 0 | 2 | 1 | 10 | 4 | 16 | 13 | 22 | 10 | 0 | 0 | 0 | 3 | 6 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 3 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 0 | 1 | 10 | 7 | 3 | 15 | 7 | 13 | 15 | 5 | 14 | 0 | 0 | 0 | 13 | 4 | 4 | 0 | 0 | 0 | 0 | 2 | 1 |
Unique Cell Count | 196 | 17 | 89 | 199 | 300 | 258 | 345 | 320 | 278 | 338 | 211 | 273 | 143 | 150 | 168 | 492 | 387 | 451 | 192 | 57 | 109 | 77 | 100 | 139 |
Labelled Cell Count | 203 | 17 | 93 | 215 | 322 | 275 | 388 | 339 | 298 | 394 | 231 | 319 | 150 | 161 | 174 | 549 | 431 | 500 | 192 | 57 | 109 | 77 | 100 | 139 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 20.7 | 23.9 | 19.9 | 21.4 | 21.7 | 20.4 | 19.2 | 18.1 | 17.1 | 17.2 | 15.5 | 16.5 | 27.6 | 26.1 | 26.1 | 27.7 | 31.4 | 30.4 | 21.3 | 22.4 | 23.8 |
Std Deviation (1e-4) | 3.7 | 3.0 | 3.6 | 4.3 | 4.8 | 4.0 | 4.0 | 4.1 | 4.1 | 4.2 | 5.0 | 4.7 | 6.1 | 4.8 | 5.0 | 6.9 | 7.7 | 6.6 | 6.3 | 4.6 | 5.8 |
Intensity Change (Log2) | – | – | – | 0.1 | 0.13 | 0.03 | -0.06 | -0.14 | -0.23 | -0.21 | -0.36 | -0.27 | 0.47 | 0.39 | 0.39 | 0.48 | 0.66 | 0.61 | 0.09 | 0.17 | 0.26 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.9 | 2.0 | 1.9 | 2.3 | 0 | 1.6 | 2.9 | 1.7 | 3.1 | 0 | 0 | 0 | 2.2 | 2.0 | 1.7 |
Cytoplasm | -2.2 | -2.0 | -2.0 | -4.1 | -3.5 | -4.5 | -4.2 | -4.0 | -4.9 | 0.4 | 1.5 | 2.8 | -1.6 | -1.1 | -0.2 |
Endoplasmic Reticulum | 1.2 | 1.2 | 1.2 | 2.6 | 2.7 | 2.3 | 2.9 | 1.2 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 17.1088 | 24.6371 | 21.5454 | 20.2858 | 17.6715 | 21.8235 | 20.225 | 26.3117 | 24.1622 | 22.0193 | 21.4336 | 22.8399 | 19.0616 | 24.5507 | 22.4691 | 21.1236 | 14.9478 | 22.3352 |
Actin | 0.0092 | 0.0002 | 0.0052 | 0.0219 | 0.0028 | 0.0015 | 0.0017 | 0.0002 | 0.0003 | 0 | 0.0004 | 0.0016 | 0.0062 | 0.0034 | 0.0125 | 0.0008 | 0.0019 | 0.0005 |
Bud | 0.0007 | 0.0001 | 0.0004 | 0.0002 | 0.0012 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.0069 | 0.0003 |
Bud Neck | 0.0006 | 0.0002 | 0.0007 | 0.0006 | 0.0005 | 0.0009 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0006 | 0.0008 | 0.0004 | 0.0001 | 0.0005 | 0.0002 | 0.0009 | 0.0006 |
Bud Periphery | 0.001 | 0 | 0.0004 | 0.0001 | 0.0031 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0077 | 0.0001 |
Bud Site | 0.0015 | 0.0002 | 0.0021 | 0.001 | 0.0007 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0014 | 0.0001 | 0.0005 | 0.0001 | 0.0043 | 0.0001 |
Cell Periphery | 0.0006 | 0.0001 | 0.0004 | 0.0001 | 0.0017 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0 |
Cytoplasm | 0.8207 | 0.9795 | 0.9153 | 0.8856 | 0.7473 | 0.9321 | 0.9297 | 0.9722 | 0.9718 | 0.9892 | 0.9515 | 0.9479 | 0.9159 | 0.982 | 0.9464 | 0.93 | 0.6896 | 0.9492 |
Cytoplasmic Foci | 0.0285 | 0.0033 | 0.0094 | 0.0106 | 0.0096 | 0.0103 | 0.0044 | 0.0011 | 0.0049 | 0.0011 | 0.0038 | 0.003 | 0.0088 | 0.0011 | 0.0041 | 0.0043 | 0.0338 | 0.0054 |
Eisosomes | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0254 | 0.0049 | 0.007 | 0.0253 | 0.015 | 0.0199 | 0.0336 | 0.0226 | 0.0108 | 0.0034 | 0.018 | 0.0341 | 0.019 | 0.0079 | 0.0103 | 0.0106 | 0.0072 | 0.03 |
Endosome | 0.0493 | 0.006 | 0.0227 | 0.0234 | 0.0459 | 0.0134 | 0.0056 | 0.0008 | 0.0028 | 0.0014 | 0.0059 | 0.0047 | 0.0176 | 0.0015 | 0.0089 | 0.0105 | 0.0995 | 0.0063 |
Golgi | 0.0117 | 0.0013 | 0.006 | 0.0105 | 0.0224 | 0.0046 | 0.0012 | 0.0001 | 0.0007 | 0 | 0.001 | 0.0016 | 0.0035 | 0.0015 | 0.0019 | 0.0089 | 0.0178 | 0.0006 |
Lipid Particles | 0.008 | 0.0003 | 0.0037 | 0.0019 | 0.0059 | 0.0006 | 0.0013 | 0 | 0.0016 | 0 | 0.0005 | 0.0005 | 0.0035 | 0 | 0.0027 | 0.0012 | 0.0285 | 0.0004 |
Mitochondria | 0.0139 | 0.0001 | 0.0171 | 0.0046 | 0.0915 | 0.0073 | 0.0024 | 0 | 0.001 | 0 | 0.0038 | 0.0007 | 0.0051 | 0.0005 | 0.0027 | 0.021 | 0.0321 | 0.0011 |
None | 0.0019 | 0.0002 | 0.0015 | 0.0004 | 0.0009 | 0.0005 | 0.0027 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0004 | 0.0017 | 0.0001 | 0.0005 | 0.0003 | 0.0012 | 0.0003 |
Nuclear Periphery | 0.005 | 0.0006 | 0.0009 | 0.0022 | 0.0238 | 0.0013 | 0.005 | 0.0006 | 0.0007 | 0.0007 | 0.0011 | 0.0013 | 0.0043 | 0.0006 | 0.002 | 0.0043 | 0.002 | 0.0016 |
Nucleolus | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0008 | 0 |
Nucleus | 0.0017 | 0.0012 | 0.0009 | 0.0011 | 0.0075 | 0.0017 | 0.003 | 0.0007 | 0.0007 | 0.001 | 0.001 | 0.0013 | 0.0019 | 0.0004 | 0.001 | 0.0011 | 0.0025 | 0.0009 |
Peroxisomes | 0.0068 | 0 | 0.0016 | 0.0047 | 0.0013 | 0.0014 | 0.0005 | 0 | 0.0006 | 0 | 0 | 0 | 0.0029 | 0 | 0.0007 | 0.0002 | 0.0199 | 0.0001 |
Punctate Nuclear | 0.0029 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.0002 | 0.0027 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0018 | 0 | 0.0006 | 0.0001 | 0.0005 | 0.0001 |
Vacuole | 0.007 | 0.0013 | 0.0035 | 0.0045 | 0.0097 | 0.0026 | 0.0041 | 0.0009 | 0.003 | 0.0021 | 0.006 | 0.0013 | 0.0037 | 0.0003 | 0.0027 | 0.0035 | 0.0293 | 0.0018 |
Vacuole Periphery | 0.0032 | 0.0001 | 0.0007 | 0.001 | 0.0081 | 0.0009 | 0.0007 | 0.0001 | 0.0002 | 0.0003 | 0.0015 | 0.0003 | 0.0012 | 0.0001 | 0.0009 | 0.0026 | 0.0126 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 288.3055 | 330.6871 | 337.491 | 387.478 | 362.1194 | 346.4107 | 362.3802 | 464.9163 | 461.4006 | 352.8765 |
Translational Efficiency | 2.1279 | 1.9362 | 1.7377 | 1.6795 | 1.5493 | 1.7593 | 1.8307 | 1.5728 | 1.6428 | 1.7209 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1229 | 841 | 2341 | 1372 | 1740 | 1711 | 229 | 1314 | 2969 | 2552 | 2570 | 2686 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1764.80 | 2216.17 | 2284.82 | 2700.29 | 1855.68 | 2240.16 | 2591.37 | 2814.45 | 1818.06 | 2232.25 | 2312.14 | 2756.14 |
Standard Deviation | 337.02 | 424.09 | 440.74 | 809.60 | 319.53 | 418.43 | 397.36 | 708.41 | 329.93 | 420.45 | 445.69 | 763.91 |
Intensity Change Log 2 | — | 0.328564 | 0.372576 | 0.613610 | — | 0.271654 | 0.481767 | 0.600905 | — | 0.299675 | 0.429573 | 0.607112 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000550 | 0.001309 | 0.000635 | 0.001756 | 0.000352 | 0.000483 | 0.001120 | 0.001809 | 0.000434 | 0.000755 | 0.000678 | 0.001782 |
Bud Neck | 0.000662 | 0.001413 | 0.001477 | 0.002275 | 0.000532 | 0.001038 | 0.009029 | 0.004334 | 0.000586 | 0.001162 | 0.002150 | 0.003282 |
Bud Site | 0.013705 | 0.018621 | 0.015781 | 0.034252 | 0.009506 | 0.015004 | 0.030421 | 0.054381 | 0.011244 | 0.016196 | 0.017086 | 0.044099 |
Cell Periphery | 0.004732 | 0.005057 | 0.007620 | 0.013644 | 0.001710 | 0.001447 | 0.019823 | 0.007986 | 0.002961 | 0.002636 | 0.008708 | 0.010876 |
Cytoplasm | 0.842194* | 0.813473* | 0.864264* | 0.799710* | 0.890948* | 0.879924* | 0.582671* | 0.703059* | 0.870767* | 0.858025* | 0.839172* | 0.752428* |
Cytoplasmic Foci | 0.040546 | 0.035853 | 0.011422 | 0.023541 | 0.028429 | 0.026829 | 0.025058 | 0.067393 | 0.033445 | 0.029803 | 0.012637 | 0.044994 |
Eisosomes | 0.000006 | 0.000006 | 0.000006 | 0.000011 | 0.000003 | 0.000003 | 0.000014 | 0.000010 | 0.000005 | 0.000004 | 0.000006 | 0.000010 |
Endoplasmic Reticulum | 0.010008 | 0.005178 | 0.000413 | 0.000365 | 0.005637 | 0.002310 | 0.003458 | 0.000307 | 0.007446 | 0.003255 | 0.000684 | 0.000337 |
Endosome | 0.004169 | 0.005345 | 0.001040 | 0.001639 | 0.002757 | 0.003477 | 0.005577 | 0.004611 | 0.003341 | 0.004092 | 0.001444 | 0.003093 |
Golgi | 0.026525 | 0.040110 | 0.006253 | 0.007498 | 0.021657 | 0.019174 | 0.029378 | 0.012994 | 0.023672 | 0.026073 | 0.008314 | 0.010187 |
Lipid Particles | 0.017714 | 0.018774 | 0.016753 | 0.023661 | 0.007168 | 0.010271 | 0.117868* | 0.028118 | 0.011533 | 0.013073 | 0.025763 | 0.025841 |
Mitochondria | 0.000818 | 0.001220 | 0.000125 | 0.001086 | 0.000200 | 0.000349 | 0.000036 | 0.000922 | 0.000456 | 0.000636 | 0.000117 | 0.001006 |
Mitotic Spindle | 0.002021 | 0.005458 | 0.000797 | 0.002524 | 0.002154 | 0.006658 | 0.003748 | 0.010203 | 0.002099 | 0.006263 | 0.001060 | 0.006281 |
None | 0.000714 | 0.000773 | 0.000811 | 0.003484 | 0.000964 | 0.001133 | 0.000586 | 0.000634 | 0.000861 | 0.001014 | 0.000791 | 0.002090 |
Nuclear Periphery | 0.000045 | 0.000101 | 0.000057 | 0.000105 | 0.000045 | 0.000091 | 0.000390 | 0.000146 | 0.000045 | 0.000094 | 0.000087 | 0.000125 |
Nuclear Periphery Foci | 0.005161 | 0.009515 | 0.003201 | 0.003961 | 0.004361 | 0.004242 | 0.025364 | 0.004212 | 0.004692 | 0.005980 | 0.005176 | 0.004084 |
Nucleolus | 0.000041 | 0.000092 | 0.000088 | 0.000473 | 0.000074 | 0.000043 | 0.000064 | 0.000116 | 0.000060 | 0.000059 | 0.000086 | 0.000299 |
Nucleus | 0.002013 | 0.003421 | 0.004785 | 0.008208 | 0.002507 | 0.003769 | 0.012455 | 0.006984 | 0.002302 | 0.003655 | 0.005469 | 0.007609 |
Peroxisomes | 0.000151 | 0.000564 | 0.000308 | 0.000730 | 0.000164 | 0.000208 | 0.000146 | 0.001322 | 0.000158 | 0.000325 | 0.000294 | 0.001020 |
Vacuole | 0.018445 | 0.021558 | 0.060648 | 0.068071 | 0.012384 | 0.016870 | 0.128000 | 0.084529 | 0.014893 | 0.018415 | 0.066649 | 0.076122 |
Vacuole Periphery | 0.009781 | 0.012158 | 0.003516 | 0.003005 | 0.008448 | 0.006677 | 0.004793 | 0.005928 | 0.009000 | 0.008483 | 0.003629 | 0.004435 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.31 | -0.57 | -2.50 | -0.53 | -2.15 | -1.01 | -4.80 | -3.20 | -2.76 | -1.40 | -1.59 | -1.47 | -4.02 | -2.45 | -2.92 |
Bud Neck | -4.24 | -4.37 | -9.03 | -4.50 | -4.19 | -3.57 | -8.64 | -13.57 | -11.39 | 4.33 | -5.23 | -8.11 | -16.19 | -11.99 | -5.69 |
Bud Site | -2.40 | -1.38 | -7.95 | -5.62 | -6.82 | -3.54 | -5.86 | -13.45 | -11.38 | -4.82 | -3.99 | -4.45 | -15.57 | -12.53 | -11.53 |
Cell Periphery | -0.32 | -3.15 | -5.97 | -5.61 | -4.15 | 1.39 | -9.76 | -8.65 | -9.18 | 6.23 | 0.78 | -9.09 | -9.62 | -10.27 | -1.88 |
Cytoplasm | 2.76 | -2.41 | 6.03 | 2.52 | 9.04 | 1.89 | 16.29 | 20.72 | 19.06 | -4.59 | 2.46 | 5.81 | 19.72 | 16.73 | 13.96 |
Cytoplasmic Foci | 1.30 | 10.95 | 2.83 | 1.67 | -5.17 | 0.44 | 0.38 | -9.73 | -9.81 | -9.23 | 1.65 | 11.16 | -6.59 | -7.42 | -12.55 |
Eisosomes | 0.42 | 0.48 | -2.97 | -3.35 | -3.39 | 0.41 | -8.87 | -4.13 | -4.47 | 2.09 | 0.95 | -1.84 | -5.30 | -6.11 | -3.32 |
Endoplasmic Reticulum | 3.19 | 7.61 | 7.65 | 5.66 | 0.55 | 3.53 | 1.24 | 6.48 | 4.28 | 1.86 | 5.09 | 9.28 | 9.99 | 6.91 | 2.14 |
Endosome | -1.91 | 8.94 | 5.65 | 5.90 | -1.74 | -1.48 | -3.47 | -2.30 | -1.12 | 1.23 | -1.97 | 7.87 | 1.03 | 2.41 | -3.98 |
Golgi | -4.46 | 12.33 | 10.17 | 11.55 | -0.73 | 1.35 | -1.45 | 5.25 | 3.85 | 4.09 | -1.43 | 13.22 | 10.70 | 10.76 | -1.14 |
Lipid Particles | -0.57 | 0.31 | -2.90 | -2.01 | -3.42 | -2.82 | -10.77 | -9.58 | -7.69 | 8.40 | -1.49 | -8.41 | -9.56 | -7.91 | -0.41 |
Mitochondria | -0.34 | 1.26 | -0.29 | 0.17 | -1.88 | -0.80 | 2.93 | -2.42 | -1.55 | -3.16 | -0.41 | 1.48 | -1.30 | -0.64 | -2.95 |
Mitotic Spindle | -2.38 | 1.63 | -1.47 | 1.22 | -2.59 | -3.62 | -2.23 | -5.16 | -2.53 | -4.37 | -4.35 | 1.75 | -5.24 | -1.16 | -6.01 |
None | -0.37 | -0.73 | -2.63 | -2.58 | -2.54 | -0.52 | 1.82 | 1.56 | 1.77 | -0.68 | -0.71 | 0.48 | -2.24 | -1.89 | -2.41 |
Nuclear Periphery | -4.25 | -1.47 | -4.94 | -1.47 | -4.38 | -1.38 | -7.94 | -6.52 | -2.14 | 4.58 | -2.13 | -5.26 | -8.19 | -2.13 | -4.59 |
Nuclear Periphery Foci | -4.54 | 3.88 | 1.47 | 5.43 | -2.35 | -0.04 | -10.10 | -0.10 | -0.06 | 10.12 | -2.71 | -1.13 | 0.70 | 3.45 | 1.90 |
Nucleolus | -0.91 | -3.10 | -1.34 | -1.17 | -1.21 | 0.66 | 0.19 | -0.90 | -6.89 | -4.45 | 0.09 | -0.74 | -1.44 | -1.47 | -1.32 |
Nucleus | -4.74 | -9.18 | -11.16 | -8.73 | -6.36 | -3.54 | -7.80 | -9.08 | -7.79 | 2.69 | -5.61 | -9.82 | -13.94 | -11.55 | -6.72 |
Peroxisomes | -1.77 | -3.53 | -3.13 | -0.29 | -2.18 | -0.48 | 0.22 | -4.69 | -4.78 | -5.11 | -1.78 | -2.09 | -5.50 | -3.82 | -4.73 |
Vacuole | -1.20 | -14.52 | -13.90 | -12.37 | -2.74 | -2.69 | -12.20 | -18.20 | -17.06 | 3.93 | -2.51 | -20.47 | -23.09 | -21.53 | -3.96 |
Vacuole Periphery | -1.13 | 5.36 | 5.55 | 5.06 | 1.00 | 1.62 | 3.62 | 2.81 | 1.23 | -0.61 | 0.54 | 7.54 | 6.07 | 4.73 | -0.72 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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