Standard name
Human Ortholog
Description Kinetochore checkpoint WD40 repeat protein; localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p; functions at kinetochore to activate APC/C-Cdc20p for normal mitotic progression
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.25 0.2 0.43 0.34 0.32 0.36 0.33 0.34 0.29 0.34 0.22 0.31 0.44 0.46 0.54 0.21 0.19 0.12 0.18 0.09 0.16 0.22 0.24 0.25
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.09 0.09 0.11 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.43 0 0 0.07 0.09 0.39 0.54 0.44 0.65 0.54 0.73 0.55 0 0 0 0.09 0 0 0.05 0 0.06 0 0.06 0
Nucleus 0.31 0.46 0.34 0.41 0.34 0.28 0.2 0.31 0.22 0.21 0.28 0.28 0.42 0.27 0.27 0.38 0.55 0.54 0.39 0.51 0.32 0.33 0.28 0.22
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.11 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.1 0.19 0.14 0.06 0.06 0.06 0 0.05 0 0.05 0.05 0.12 0.07 0.16 0.06 0.09 0.1 0.07 0.13 0 0 0.06
Vac/Vac Membrane 0.21 0.4 0.06 0.07 0.16 0.1 0.07 0.06 0.06 0.09 0 0.07 0.06 0.12 0.09 0.2 0.17 0.2 0.22 0.2 0.25 0.3 0.29 0.31
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 4
Bud 0 0 0 0 0 0 4 3 4 2 8 6 0 0 0 0 0 0 2 4 1 4 13 9
Bud Neck 0 0 3 2 8 1 4 8 2 1 0 3 5 8 5 0 0 0 0 1 0 1 9 13
Bud Site 0 0 0 0 0 2 5 8 4 6 7 6 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 41 32 42 46 68 84 95 118 64 79 70 73 116 180 234 27 34 24 38 17 24 59 118 123
Endoplasmic Reticulum 2 0 1 0 2 1 1 0 1 0 0 1 0 3 2 10 5 9 0 0 0 0 2 3
Endosome 3 7 3 6 7 4 0 2 2 2 0 1 7 32 32 12 16 21 3 4 1 1 5 6
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 1 1 2 1 0 1 0 1 0 0 1 1
Mitochondria 71 4 3 10 19 92 156 155 144 124 227 130 9 0 3 12 5 8 9 5 9 11 27 20
Nucleus 51 73 33 55 71 67 58 109 50 49 87 65 110 107 117 50 97 103 83 98 49 88 136 110
Nuclear Periphery 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 2 1
Nucleolus 4 1 1 2 1 1 1 1 2 3 2 1 4 2 2 11 12 22 0 1 1 0 1 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 7 3 10 25 30 15 17 20 5 12 2 11 14 47 30 21 11 17 20 14 21 9 20 31
Vac/Vac Membrane 35 63 6 10 33 23 20 20 13 20 14 16 15 47 38 26 30 39 45 39 39 82 142 151
Unique Cell Count 164 159 98 134 210 236 287 350 223 231 312 236 263 392 433 130 176 192 211 193 158 270 498 493
Labelled Cell Count 214 183 102 156 239 290 362 445 292 299 417 314 285 428 467 170 210 245 211 193 158 270 498 493


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 5.0 5.2 5.1 4.8 3.9 3.6 4.1 3.8 3.8 3.2 3.7 4.8 5.4 5.3 5.8 6.6 6.4 4.9 5.3 5.5
Std Deviation (1e-4) 0.9 1.3 1.6 2.5 1.3 1.6 1.3 1.5 2.8 1.8 0.7 0.8 1.1 1.4 1.3 1.5 1.8 1.5 1.7 1.8 2.1
Intensity Change (Log2) – – – -0.03 -0.11 -0.43 -0.52 -0.33 -0.45 -0.44 -0.71 -0.49 -0.11 0.05 0.04 0.18 0.36 0.3 -0.09 0.04 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.3 -1.8 -1.2 -1.7 -1.7 -2.5 -1.5 -4.0 -2.1 0.2 0.5 2.0 -3.6 -4.2 -5.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 1.8 1.6 1.9 1.9 2.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.4 0 0 0 0 0 0 0 0 0 0 0 1.9 0 0
Nucleus 1.1 0 -1.0 -2.7 -0.5 -2.1 -2.4 -1.1 -1.1 1.4 -1.2 -1.3 0.7 3.4 3.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 2.5 0 3.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1.8 0 0 0 0 0 0 0 0 0 0.5 -1.1 1.3 -1.2 -0.4
Vacuole 0.4 2.4 1.1 0.3 -0.2 -0.1 0.8 0 0.2 -0.2 1.7 0.9 3.0 2.6 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7603 -0.1122 -0.6005 0.0914 -1.1146 -0.4811 0.5561 0.2925 0.4045 -0.2154 0.2849 0.2239 -0.4436 -0.1832 -0.1464 -0.2255 -0.1178 -0.2317
Actin 0.0045 0.0002 0.0005 0.0006 0.0001 0.0002 0.0279 0.001 0.0142 0.0006 0.0089 0.0018 0.0105 0.0001 0.0001 0.0001 0.0018 0.0031
Bud 0.0003 0.0018 0 0.0001 0 0.0018 0.0008 0.0003 0.0014 0 0.0001 0.0002 0.0002 0.0001 0 0.0001 0 0.0001
Bud Neck 0.0017 0.0006 0.0009 0.0006 0.0006 0.0022 0.0052 0.0011 0.0015 0.0004 0.0006 0.0027 0.0019 0.0006 0.0007 0.0003 0.0005 0.0028
Bud Periphery 0.0003 0.0003 0 0 0 0.0005 0.0015 0.0003 0.0014 0 0.0005 0.0006 0.0002 0 0 0.0001 0.0001 0.0001
Bud Site 0.0019 0.0017 0.0002 0.0001 0 0.0001 0.0051 0.0023 0.0093 0 0.0001 0.0003 0.0006 0.0007 0.0003 0.0001 0.0002 0.0001
Cell Periphery 0.0001 0 0 0 0 0 0.0005 0.0001 0.0001 0 0.0001 0 0.0001 0 0 0 0 0
Cytoplasm 0.0578 0.0705 0.0253 0.0205 0.0329 0.0414 0.0587 0.0772 0.0353 0.0071 0.0465 0.0287 0.0744 0.0687 0.0262 0.0294 0.0251 0.0281
Cytoplasmic Foci 0.0085 0.0026 0.0042 0.0009 0.0008 0.0046 0.0225 0.0091 0.0098 0.0001 0.0038 0.0053 0.007 0.0065 0.0035 0.0007 0.0025 0.0013
Eisosomes 0.0002 0 0 0 0 0 0.0003 0 0.0002 0 0.0004 0 0.0001 0 0 0 0.0002 0.0001
Endoplasmic Reticulum 0.0021 0.0019 0.0005 0.0015 0.0003 0.0007 0.0056 0.0017 0.0026 0.0005 0.0014 0.0011 0.0028 0.0018 0.0006 0.0005 0.0008 0.0009
Endosome 0.0073 0.0031 0.0021 0.0025 0.0007 0.0025 0.0371 0.0047 0.0158 0.0007 0.0048 0.0051 0.0082 0.0025 0.0011 0.0008 0.0055 0.0022
Golgi 0.0012 0.0001 0.0004 0.0001 0 0.0001 0.0107 0.0003 0.0047 0 0.0087 0.0008 0.0014 0.0001 0.0001 0 0.0006 0.0004
Lipid Particles 0.005 0.0003 0.001 0.0001 0.0001 0.0003 0.0259 0.001 0.0036 0 0.0031 0.0036 0.0047 0.0004 0.0004 0 0.0035 0.0007
Mitochondria 0.0012 0.0007 0.0048 0.0009 0.0003 0.0004 0.0127 0.0014 0.0071 0.0015 0.0702 0.0041 0.0011 0.0004 0.0002 0.0004 0.0011 0.0006
None 0.15 0.1491 0.0997 0.1402 0.0844 0.0866 0.1216 0.1444 0.0528 0.0112 0.0784 0.0728 0.1137 0.1019 0.071 0.0128 0.0489 0.051
Nuclear Periphery 0.0233 0.0248 0.0198 0.0247 0.0176 0.012 0.0156 0.011 0.0123 0.0298 0.0157 0.0229 0.0188 0.013 0.0082 0.0095 0.0153 0.0184
Nucleolus 0.0131 0.0156 0.0145 0.0057 0.0088 0.0146 0.0173 0.0189 0.0227 0.006 0.006 0.0188 0.0127 0.0136 0.0112 0.0046 0.0102 0.0126
Nucleus 0.5487 0.6069 0.5797 0.7275 0.7544 0.6791 0.5367 0.5726 0.5848 0.9125 0.6288 0.7287 0.6192 0.6517 0.7008 0.8913 0.7791 0.7505
Peroxisomes 0.0028 0.0001 0.0013 0.0001 0 0.0001 0.0168 0.0003 0.0022 0.0001 0.0006 0.0046 0.0053 0.0001 0.0001 0 0.0006 0.0001
Punctate Nuclear 0.168 0.1168 0.2445 0.0726 0.0985 0.1519 0.0706 0.15 0.2155 0.0288 0.1193 0.0962 0.1144 0.1365 0.1747 0.0489 0.1028 0.1263
Vacuole 0.0013 0.0024 0.0004 0.0007 0.0003 0.0007 0.0051 0.0019 0.0017 0.0002 0.0007 0.0009 0.0022 0.001 0.0005 0.0003 0.0006 0.0005
Vacuole Periphery 0.0005 0.0005 0.0002 0.0006 0.0001 0.0002 0.0016 0.0004 0.001 0.0004 0.0013 0.001 0.0006 0.0002 0.0002 0.0002 0.0005 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.5843 13.4593 17.8346 17.9711 20.9154 22.5718 19.441 23.6185 16.4382 24.2998
Translational Efficiency 0.6742 0.4819 0.1179 0.2659 0.5585 0.6359 0.2328 0.1695 0.2878 0.4849

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1554 588 2120 1012 1806 1265 600 1119 3360 1853 2720 2131

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 664.42 758.23 771.54 774.48 660.55 731.02 832.55 833.59 662.34 739.65 785.00 805.52
Standard Deviation 78.01 105.67 98.89 106.91 70.48 88.35 104.83 120.32 74.08 95.04 103.38 117.90
Intensity Change Log 2 — 0.190540 0.215645 0.221132 — 0.146243 0.333869 0.335670 — 0.168626 0.275795 0.279371

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000103 0.001370 0.000255 0.001878 0.000071 0.001072 0.000430 0.003296 0.000086 0.001167 0.000294 0.002622
Bud Neck 0.004264 0.022982 0.010083 0.019932 0.004413 0.015108 0.001680 0.011069 0.004344 0.017607 0.008229 0.015278
Bud Site 0.001543 0.016813 0.003361 0.082982 0.000785 0.011205 0.000867 0.040670 0.001136 0.012985 0.002811 0.060764
Cell Periphery 0.000151 0.001152 0.000079 0.000378 0.000118 0.000333 0.000217 0.000231 0.000133 0.000593 0.000110 0.000301
Cytoplasm 0.224318* 0.188366* 0.038496 0.200216* 0.211668* 0.208216* 0.060528 0.227107* 0.217519* 0.201917* 0.043356 0.214337*
Cytoplasmic Foci 0.083911 0.082316 0.000295 0.010379 0.046140 0.051208 0.007438 0.012536 0.063609 0.061079 0.001871 0.011511
Eisosomes 0.000075 0.000639 0.000012 0.000057 0.000046 0.000064 0.000057 0.000049 0.000060 0.000246 0.000022 0.000053
Endoplasmic Reticulum 0.001159 0.006588 0.002920 0.007755 0.000900 0.001879 0.003188 0.005739 0.001020 0.003374 0.002979 0.006697
Endosome 0.002974 0.012824 0.000285 0.006344 0.001410 0.004507 0.001255 0.013757 0.002133 0.007146 0.000499 0.010236
Golgi 0.001171 0.003361 0.000035 0.006919 0.000345 0.001869 0.000910 0.006930 0.000727 0.002342 0.000228 0.006925
Lipid Particles 0.010607 0.009277 0.000168 0.001367 0.005147 0.005790 0.001059 0.001631 0.007672 0.006897 0.000365 0.001506
Mitochondria 0.006770 0.014566 0.000844 0.012480 0.003509 0.007156 0.000691 0.004968 0.005017 0.009507 0.000810 0.008535
Mitotic Spindle 0.001205 0.029364 0.001382 0.046352 0.001659 0.013724 0.001138 0.065997 0.001449 0.018687 0.001328 0.056668
None 0.012392 0.012299 0.004624 0.008841 0.007115 0.011160 0.011897 0.010918 0.009555 0.011522 0.006229 0.009931
Nuclear Periphery 0.002126 0.002172 0.001238 0.003041 0.001471 0.001651 0.000802 0.003055 0.001774 0.001817 0.001142 0.003049
Nuclear Periphery Foci 0.001066 0.002873 0.000227 0.002750 0.000723 0.000975 0.000306 0.002070 0.000881 0.001578 0.000244 0.002393
Nucleolus 0.009326 0.011298 0.001372 0.002347 0.006820 0.012500 0.001754 0.001558 0.007979 0.012119 0.001456 0.001932
Nucleus 0.623235* 0.523191* 0.930530* 0.526735* 0.699511* 0.630352* 0.899535* 0.533391* 0.664233* 0.596348* 0.923693* 0.530230*
Peroxisomes 0.006578 0.020677 0.000048 0.005551 0.002388 0.007640 0.000414 0.003128 0.004326 0.011777 0.000129 0.004279
Vacuole 0.006601 0.035099 0.003650 0.047990 0.005553 0.012707 0.005621 0.048985 0.006038 0.019812 0.004085 0.048512
Vacuole Periphery 0.000424 0.002772 0.000094 0.005708 0.000211 0.000884 0.000212 0.002915 0.000309 0.001483 0.000120 0.004241

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.98 -10.95 -9.89 -0.01 -7.53 -1.35 -4.51 -4.90 -0.98 -2.25 -2.40 -10.08 -8.38 -0.99 -5.81
Bud Neck -7.65 -8.84 -10.13 0.60 -3.10 -6.32 0.70 -7.87 0.30 -8.36 -9.75 -8.29 -12.77 -0.09 -3.76
Bud Site -6.29 -3.06 -9.62 -5.48 -8.98 -5.41 -3.31 -8.42 -3.48 -7.64 -7.79 -4.49 -12.52 -6.85 -11.23
Cell Periphery -1.13 6.54 -4.63 0.95 -7.07 -2.04 -2.91 -3.53 1.47 0.88 -1.60 2.61 -5.78 1.23 -6.89
Cytoplasm 3.36 30.40 6.91 2.29 -18.16 0.50 22.63 5.38 4.39 -14.24 2.38 41.43 8.65 5.21 -25.06
Cytoplasmic Foci 1.25 23.42 20.53 12.53 -7.04 -0.37 15.27 15.62 14.53 -1.36 1.93 29.14 25.53 19.06 -8.98
Eisosomes -1.10 16.97 -0.43 1.10 -14.09 -3.32 -2.76 -5.01 -1.10 -0.34 -1.15 12.22 -3.76 1.06 -14.40
Endoplasmic Reticulum -10.24 -14.05 -16.28 -5.73 -11.31 -4.57 -11.16 -17.42 -13.99 -3.04 -10.31 -19.78 -23.34 -14.83 -12.60
Endosome -5.62 5.91 -2.07 4.73 -8.22 -5.35 0.69 -4.66 -1.34 -4.54 -7.16 6.75 -5.08 2.13 -8.54
Golgi -2.55 5.32 -1.34 0.55 -2.58 -2.20 -0.43 -2.15 -0.32 -1.57 -2.90 3.69 -2.46 -0.09 -3.39
Lipid Particles 1.16 13.25 10.87 8.94 -13.58 -1.21 8.42 7.59 8.09 -2.66 1.00 16.57 13.18 11.66 -14.92
Mitochondria -2.58 4.27 0.48 3.01 -4.17 -3.12 3.27 0.79 4.53 -4.65 -3.50 5.33 0.77 4.47 -5.56
Mitotic Spindle -6.04 -2.36 -10.49 -3.52 -10.04 -5.35 -1.68 -11.01 -7.58 -9.91 -8.03 -2.93 -15.20 -8.59 -14.42
None 0.86 6.53 3.76 1.60 -4.40 -2.90 -1.18 -1.27 1.91 0.53 -1.04 5.34 2.22 2.62 -3.64
Nuclear Periphery -0.26 0.64 -5.07 -6.15 -7.27 -1.10 -0.33 -13.20 -11.49 -13.30 -0.39 0.20 -9.82 -11.24 -12.36
Nuclear Periphery Foci -2.75 0.69 -6.01 -4.21 -6.22 -0.26 -0.32 -3.90 -3.71 -3.23 -1.97 0.22 -7.10 -6.03 -6.97
Nucleolus 0.12 4.54 5.00 3.66 0.61 -2.02 4.18 4.83 6.01 0.28 -1.60 5.88 6.93 7.01 1.06
Nucleus 6.11 -35.21 4.67 -2.08 33.03 5.92 -19.08 9.57 3.57 24.62 7.09 -42.07 10.17 2.34 43.87
Peroxisomes -5.14 9.60 4.64 6.97 -2.95 -5.25 6.42 3.81 7.03 -2.50 -6.62 12.15 5.76 9.44 -4.18
Vacuole -9.11 0.50 -17.04 -9.02 -17.03 -6.28 -3.99 -18.93 -17.53 -15.26 -10.85 -2.76 -25.42 -20.50 -24.37
Vacuole Periphery -4.12 4.50 -2.73 0.34 -3.20 -4.18 0.12 -2.86 -0.63 -2.77 -5.54 4.15 -3.73 -0.59 -4.23
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Kinetochore checkpoint WD40 repeat protein; localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p; functions at kinetochore to activate APC/C-Cdc20p for normal mitotic progression
Localization
Cell Percentages nucleus (57%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Bub3

Bub3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bub3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available