Standard name
Human Ortholog
Description Kinetochore checkpoint WD40 repeat protein; localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p; functions at kinetochore to activate APC/C-Cdc20p for normal mitotic progression
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.25 0.2 0.43 0.34 0.32 0.36 0.33 0.34 0.29 0.34 0.22 0.31 0.44 0.46 0.54 0.21 0.19 0.12 0.18 0.09 0.16 0.22 0.24 0.25
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.09 0.09 0.11 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.43 0 0 0.07 0.09 0.39 0.54 0.44 0.65 0.54 0.73 0.55 0 0 0 0.09 0 0 0.05 0 0.06 0 0.06 0
Nucleus 0.31 0.46 0.34 0.41 0.34 0.28 0.2 0.31 0.22 0.21 0.28 0.28 0.42 0.27 0.27 0.38 0.55 0.54 0.39 0.51 0.32 0.33 0.28 0.22
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.11 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.1 0.19 0.14 0.06 0.06 0.06 0 0.05 0 0.05 0.05 0.12 0.07 0.16 0.06 0.09 0.1 0.07 0.13 0 0 0.06
Vac/Vac Membrane 0.21 0.4 0.06 0.07 0.16 0.1 0.07 0.06 0.06 0.09 0 0.07 0.06 0.12 0.09 0.2 0.17 0.2 0.22 0.2 0.25 0.3 0.29 0.31
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 4
Bud 0 0 0 0 0 0 4 3 4 2 8 6 0 0 0 0 0 0 2 4 1 4 13 9
Bud Neck 0 0 3 2 8 1 4 8 2 1 0 3 5 8 5 0 0 0 0 1 0 1 9 13
Bud Site 0 0 0 0 0 2 5 8 4 6 7 6 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 41 32 42 46 68 84 95 118 64 79 70 73 116 180 234 27 34 24 38 17 24 59 118 123
Endoplasmic Reticulum 2 0 1 0 2 1 1 0 1 0 0 1 0 3 2 10 5 9 0 0 0 0 2 3
Endosome 3 7 3 6 7 4 0 2 2 2 0 1 7 32 32 12 16 21 3 4 1 1 5 6
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 1 1 2 1 0 1 0 1 0 0 1 1
Mitochondria 71 4 3 10 19 92 156 155 144 124 227 130 9 0 3 12 5 8 9 5 9 11 27 20
Nucleus 51 73 33 55 71 67 58 109 50 49 87 65 110 107 117 50 97 103 83 98 49 88 136 110
Nuclear Periphery 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 2 1
Nucleolus 4 1 1 2 1 1 1 1 2 3 2 1 4 2 2 11 12 22 0 1 1 0 1 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 7 3 10 25 30 15 17 20 5 12 2 11 14 47 30 21 11 17 20 14 21 9 20 31
Vac/Vac Membrane 35 63 6 10 33 23 20 20 13 20 14 16 15 47 38 26 30 39 45 39 39 82 142 151
Unique Cell Count 164 159 98 134 210 236 287 350 223 231 312 236 263 392 433 130 176 192 211 193 158 270 498 493
Labelled Cell Count 214 183 102 156 239 290 362 445 292 299 417 314 285 428 467 170 210 245 211 193 158 270 498 493


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 5.0 5.2 5.1 4.8 3.9 3.6 4.1 3.8 3.8 3.2 3.7 4.8 5.4 5.3 5.8 6.6 6.4 4.9 5.3 5.5
Std Deviation (1e-4) 0.9 1.3 1.6 2.5 1.3 1.6 1.3 1.5 2.8 1.8 0.7 0.8 1.1 1.4 1.3 1.5 1.8 1.5 1.7 1.8 2.1
Intensity Change (Log2) – – – -0.03 -0.11 -0.43 -0.52 -0.33 -0.45 -0.44 -0.71 -0.49 -0.11 0.05 0.04 0.18 0.36 0.3 -0.09 0.04 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.3 -1.8 -1.2 -1.7 -1.7 -2.5 -1.5 -4.0 -2.1 0.2 0.5 2.0 -3.6 -4.2 -5.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 1.8 1.6 1.9 1.9 2.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.4 0 0 0 0 0 0 0 0 0 0 0 1.9 0 0
Nucleus 1.1 0 -1.0 -2.7 -0.5 -2.1 -2.4 -1.1 -1.1 1.4 -1.2 -1.3 0.7 3.4 3.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 2.5 0 3.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1.8 0 0 0 0 0 0 0 0 0 0.5 -1.1 1.3 -1.2 -0.4
Vacuole 0.4 2.4 1.1 0.3 -0.2 -0.1 0.8 0 0.2 -0.2 1.7 0.9 3.0 2.6 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7603 -0.1122 -0.6005 0.0914 -1.1146 -0.4811 0.5561 0.2925 0.4045 -0.2154 0.2849 0.2239 -0.4436 -0.1832 -0.1464 -0.2255 -0.1178 -0.2317
Actin 0.0045 0.0002 0.0005 0.0006 0.0001 0.0002 0.0279 0.001 0.0142 0.0006 0.0089 0.0018 0.0105 0.0001 0.0001 0.0001 0.0018 0.0031
Bud 0.0003 0.0018 0 0.0001 0 0.0018 0.0008 0.0003 0.0014 0 0.0001 0.0002 0.0002 0.0001 0 0.0001 0 0.0001
Bud Neck 0.0017 0.0006 0.0009 0.0006 0.0006 0.0022 0.0052 0.0011 0.0015 0.0004 0.0006 0.0027 0.0019 0.0006 0.0007 0.0003 0.0005 0.0028
Bud Periphery 0.0003 0.0003 0 0 0 0.0005 0.0015 0.0003 0.0014 0 0.0005 0.0006 0.0002 0 0 0.0001 0.0001 0.0001
Bud Site 0.0019 0.0017 0.0002 0.0001 0 0.0001 0.0051 0.0023 0.0093 0 0.0001 0.0003 0.0006 0.0007 0.0003 0.0001 0.0002 0.0001
Cell Periphery 0.0001 0 0 0 0 0 0.0005 0.0001 0.0001 0 0.0001 0 0.0001 0 0 0 0 0
Cytoplasm 0.0578 0.0705 0.0253 0.0205 0.0329 0.0414 0.0587 0.0772 0.0353 0.0071 0.0465 0.0287 0.0744 0.0687 0.0262 0.0294 0.0251 0.0281
Cytoplasmic Foci 0.0085 0.0026 0.0042 0.0009 0.0008 0.0046 0.0225 0.0091 0.0098 0.0001 0.0038 0.0053 0.007 0.0065 0.0035 0.0007 0.0025 0.0013
Eisosomes 0.0002 0 0 0 0 0 0.0003 0 0.0002 0 0.0004 0 0.0001 0 0 0 0.0002 0.0001
Endoplasmic Reticulum 0.0021 0.0019 0.0005 0.0015 0.0003 0.0007 0.0056 0.0017 0.0026 0.0005 0.0014 0.0011 0.0028 0.0018 0.0006 0.0005 0.0008 0.0009
Endosome 0.0073 0.0031 0.0021 0.0025 0.0007 0.0025 0.0371 0.0047 0.0158 0.0007 0.0048 0.0051 0.0082 0.0025 0.0011 0.0008 0.0055 0.0022
Golgi 0.0012 0.0001 0.0004 0.0001 0 0.0001 0.0107 0.0003 0.0047 0 0.0087 0.0008 0.0014 0.0001 0.0001 0 0.0006 0.0004
Lipid Particles 0.005 0.0003 0.001 0.0001 0.0001 0.0003 0.0259 0.001 0.0036 0 0.0031 0.0036 0.0047 0.0004 0.0004 0 0.0035 0.0007
Mitochondria 0.0012 0.0007 0.0048 0.0009 0.0003 0.0004 0.0127 0.0014 0.0071 0.0015 0.0702 0.0041 0.0011 0.0004 0.0002 0.0004 0.0011 0.0006
None 0.15 0.1491 0.0997 0.1402 0.0844 0.0866 0.1216 0.1444 0.0528 0.0112 0.0784 0.0728 0.1137 0.1019 0.071 0.0128 0.0489 0.051
Nuclear Periphery 0.0233 0.0248 0.0198 0.0247 0.0176 0.012 0.0156 0.011 0.0123 0.0298 0.0157 0.0229 0.0188 0.013 0.0082 0.0095 0.0153 0.0184
Nucleolus 0.0131 0.0156 0.0145 0.0057 0.0088 0.0146 0.0173 0.0189 0.0227 0.006 0.006 0.0188 0.0127 0.0136 0.0112 0.0046 0.0102 0.0126
Nucleus 0.5487 0.6069 0.5797 0.7275 0.7544 0.6791 0.5367 0.5726 0.5848 0.9125 0.6288 0.7287 0.6192 0.6517 0.7008 0.8913 0.7791 0.7505
Peroxisomes 0.0028 0.0001 0.0013 0.0001 0 0.0001 0.0168 0.0003 0.0022 0.0001 0.0006 0.0046 0.0053 0.0001 0.0001 0 0.0006 0.0001
Punctate Nuclear 0.168 0.1168 0.2445 0.0726 0.0985 0.1519 0.0706 0.15 0.2155 0.0288 0.1193 0.0962 0.1144 0.1365 0.1747 0.0489 0.1028 0.1263
Vacuole 0.0013 0.0024 0.0004 0.0007 0.0003 0.0007 0.0051 0.0019 0.0017 0.0002 0.0007 0.0009 0.0022 0.001 0.0005 0.0003 0.0006 0.0005
Vacuole Periphery 0.0005 0.0005 0.0002 0.0006 0.0001 0.0002 0.0016 0.0004 0.001 0.0004 0.0013 0.001 0.0006 0.0002 0.0002 0.0002 0.0005 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.5843 13.4593 17.8346 17.9711 20.9154 22.5718 19.441 23.6185 16.4382 24.2998
Translational Efficiency 0.6742 0.4819 0.1179 0.2659 0.5585 0.6359 0.2328 0.1695 0.2878 0.4849

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✔
Standard name
Human Ortholog
Description Kinetochore checkpoint WD40 repeat protein; localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p; functions at kinetochore to activate APC/C-Cdc20p for normal mitotic progression
Localization
Cell Percentages nucleus (57%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Bub3

Bub3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bub3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available