Standard name | |
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Human Ortholog | |
Description | Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | 0 | 2 | 1 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 1 | 2 | 0 | 3 | 0 | 9 | 4 | 13 | 6 | – | 0 | 0 | – | – | – | 2 | 4 | 2 | 0 | 3 | 2 |
Bud Neck | 1 | 0 | 2 | 2 | 6 | 5 | 7 | 15 | 7 | 11 | 6 | 11 | – | 0 | 0 | – | – | – | 2 | 4 | 0 | 1 | 1 | 3 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 2 | 5 | 4 | – | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | – | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 68 | 134 | 89 | 92 | 277 | 261 | 461 | 398 | 270 | 286 | 185 | 207 | – | 31 | 75 | – | – | – | 60 | 134 | 83 | 8 | 62 | 87 |
Endoplasmic Reticulum | 0 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | – | – | – | 0 | 1 | 2 | 0 | 1 | 1 |
Endosome | 2 | 9 | 9 | 7 | 12 | 4 | 9 | 6 | 2 | 7 | 5 | 13 | – | 3 | 2 | – | – | – | 2 | 5 | 7 | 0 | 2 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | 0 | 4 | 0 | 0 | 0 | 0 |
Mitochondria | 7 | 48 | 3 | 21 | 8 | 44 | 89 | 77 | 281 | 243 | 324 | 331 | – | 1 | 2 | – | – | – | 9 | 11 | 19 | 0 | 5 | 3 |
Nucleus | 26 | 36 | 33 | 51 | 15 | 11 | 23 | 29 | 57 | 105 | 112 | 164 | – | 47 | 56 | – | – | – | 44 | 92 | 65 | 15 | 25 | 13 |
Nuclear Periphery | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | – | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | – | 0 | 0 | – | – | – | 0 | 1 | 3 | 3 | 3 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 4 | 11 | 4 | 1 | 12 | 10 | 3 | 14 | 12 | 28 | – | 1 | 0 | – | – | – | 9 | 24 | 21 | 3 | 13 | 11 |
Vac/Vac Membrane | 36 | 89 | 28 | 18 | 41 | 29 | 25 | 35 | 17 | 14 | 25 | 54 | – | 8 | 22 | – | – | – | 44 | 87 | 53 | 3 | 22 | 17 |
Unique Cell Count | 131 | 283 | 161 | 183 | 340 | 331 | 588 | 521 | 541 | 598 | 547 | 668 | 89 | 152 | 187 | 386 | 276 | 39 | 148 | 148 | ||||
Labelled Cell Count | 141 | 324 | 168 | 205 | 365 | 356 | 631 | 572 | 653 | 688 | 688 | 822 | 92 | 157 | 187 | 386 | 276 | 39 | 148 | 148 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.1 | 3.8 | 5.1 | 4.7 | 5.1 | 4.2 | 4.1 | 4.3 | 3.3 | 3.8 | 3.6 | 3.9 | – | 5.2 | 4.8 | 3.6 | 4.8 | 4.7 | – | 5.9 | 5.7 |
Std Deviation (1e-4) | 0.6 | 0.8 | 1.0 | 1.3 | 1.6 | 1.3 | 1.2 | 1.2 | 0.9 | 1.1 | 0.9 | 1.1 | – | 1.1 | 1.1 | 0.5 | 2.0 | 0.8 | – | 2.3 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.01 | -0.3 | -0.33 | -0.27 | -0.62 | -0.44 | -0.51 | -0.41 | – | 0.02 | -0.08 | -0.49 | -0.08 | -0.11 | – | 0.19 | 0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2354 | 1.5875 | 1.2881 | 1.369 | 0.7742 | 1.3003 | 2.0579 | 2.5567 | 2.6978 | 2.2696 | 2.169 | 2.4737 | -0.492 | -0.4683 | -0.4834 | -0.3587 | -0.4341 | -0.3677 |
Actin | 0.0372 | 0.0003 | 0.0015 | 0.024 | 0.0004 | 0.0002 | 0.0014 | 0.0047 | 0.002 | 0.0025 | 0.0028 | 0.0003 | 0.0169 | 0.0004 | 0.0063 | 0.0265 | 0.059 | 0.0007 |
Bud | 0.0006 | 0.0001 | 0.0004 | 0.0101 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0005 | 0.0003 | 0.0025 | 0 | 0.0008 | 0.0002 | 0.0008 | 0.0003 | 0.0006 | 0.0008 |
Bud Neck | 0.0145 | 0.0003 | 0.0007 | 0.0007 | 0.0012 | 0.0026 | 0.0086 | 0.0005 | 0.0011 | 0.0007 | 0.0028 | 0.002 | 0.0039 | 0.0006 | 0.0007 | 0.0008 | 0.0016 | 0.0027 |
Bud Periphery | 0.001 | 0.0001 | 0.001 | 0.0033 | 0.0001 | 0.0001 | 0.0015 | 0.0002 | 0.003 | 0.0007 | 0.0139 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0005 | 0.0016 | 0.0003 |
Bud Site | 0.0106 | 0.0007 | 0.0017 | 0.0109 | 0.0003 | 0.0002 | 0.0017 | 0.0004 | 0.0048 | 0.0009 | 0.0056 | 0.0001 | 0.0133 | 0.0014 | 0.0004 | 0.0005 | 0.0009 | 0.0004 |
Cell Periphery | 0.0015 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0027 | 0.0001 | 0.0016 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.0737 | 0.0828 | 0.0377 | 0.0166 | 0.038 | 0.0401 | 0.0312 | 0.0059 | 0.0316 | 0.0479 | 0.014 | 0.0214 | 0.1117 | 0.1637 | 0.1197 | 0.1291 | 0.0657 | 0.1472 |
Cytoplasmic Foci | 0.0173 | 0.0017 | 0.0093 | 0.042 | 0.0013 | 0.0011 | 0.003 | 0.0002 | 0.0046 | 0.0058 | 0.0233 | 0.0014 | 0.0101 | 0.0066 | 0.0067 | 0.0019 | 0.0015 | 0.0047 |
Eisosomes | 0.0011 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0013 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0048 | 0.003 | 0.0016 | 0.0012 | 0.0016 | 0.0007 | 0.0029 | 0.0016 | 0.0029 | 0.0029 | 0.0024 | 0.0009 | 0.0046 | 0.0032 | 0.0034 | 0.0039 | 0.0051 | 0.0021 |
Endosome | 0.017 | 0.0013 | 0.003 | 0.0224 | 0.0022 | 0.0009 | 0.0138 | 0.0014 | 0.0042 | 0.016 | 0.0113 | 0.0018 | 0.014 | 0.0042 | 0.0058 | 0.0045 | 0.0103 | 0.0045 |
Golgi | 0.0117 | 0.0001 | 0.0007 | 0.0066 | 0.0001 | 0 | 0.0015 | 0.0002 | 0.001 | 0.0061 | 0.0063 | 0 | 0.0051 | 0.0002 | 0.0011 | 0.0027 | 0.0032 | 0.0007 |
Lipid Particles | 0.0203 | 0.0003 | 0.0026 | 0.0177 | 0.0011 | 0.0001 | 0.0044 | 0 | 0.0071 | 0.0311 | 0.0193 | 0.0002 | 0.0072 | 0.0005 | 0.0061 | 0.0008 | 0.0014 | 0.0011 |
Mitochondria | 0.0105 | 0.0005 | 0.0052 | 0.0029 | 0.0006 | 0.0004 | 0.0052 | 0.0013 | 0.0101 | 0.0328 | 0.0199 | 0.0003 | 0.0027 | 0.0005 | 0.0014 | 0.0023 | 0.0055 | 0.0017 |
None | 0.1668 | 0.1981 | 0.1515 | 0.1527 | 0.2052 | 0.1285 | 0.07 | 0.0379 | 0.0851 | 0.006 | 0.1848 | 0.064 | 0.1017 | 0.1105 | 0.1335 | 0.0826 | 0.0815 | 0.0756 |
Nuclear Periphery | 0.0147 | 0.0137 | 0.0116 | 0.0164 | 0.0075 | 0.0054 | 0.0353 | 0.029 | 0.0139 | 0.0273 | 0.005 | 0.0129 | 0.021 | 0.0176 | 0.0293 | 0.0093 | 0.0518 | 0.0095 |
Nucleolus | 0.007 | 0.0058 | 0.0055 | 0.0104 | 0.014 | 0.0116 | 0.0123 | 0.0088 | 0.0101 | 0.0058 | 0.0252 | 0.0175 | 0.006 | 0.0036 | 0.0035 | 0.0033 | 0.0056 | 0.0067 |
Nucleus | 0.5425 | 0.6699 | 0.7232 | 0.6194 | 0.6895 | 0.7898 | 0.762 | 0.8972 | 0.7728 | 0.7974 | 0.5938 | 0.826 | 0.6443 | 0.6599 | 0.6456 | 0.7199 | 0.6852 | 0.7084 |
Peroxisomes | 0.0187 | 0.0001 | 0.0021 | 0.0173 | 0.0002 | 0 | 0.0008 | 0.0001 | 0.009 | 0.0063 | 0.0282 | 0.0001 | 0.0032 | 0.0004 | 0.0031 | 0.0002 | 0.0006 | 0.0013 |
Punctate Nuclear | 0.0245 | 0.0199 | 0.0366 | 0.0238 | 0.0343 | 0.0177 | 0.0319 | 0.0096 | 0.0267 | 0.0054 | 0.0204 | 0.0498 | 0.0275 | 0.0235 | 0.0304 | 0.0088 | 0.0152 | 0.0292 |
Vacuole | 0.0029 | 0.0008 | 0.0026 | 0.0009 | 0.0012 | 0.0006 | 0.0083 | 0.0004 | 0.0054 | 0.002 | 0.0121 | 0.0006 | 0.004 | 0.0024 | 0.0014 | 0.0015 | 0.002 | 0.002 |
Vacuole Periphery | 0.0011 | 0.0002 | 0.0006 | 0.0005 | 0.0002 | 0.0001 | 0.0031 | 0.0004 | 0.001 | 0.0017 | 0.0035 | 0.0004 | 0.0008 | 0.0005 | 0.0005 | 0.0004 | 0.0016 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.2129 | 4.4431 | 7.4182 | 7.3679 | 4.0276 | 8.6932 | 4.2117 | 7.5803 | 6.5288 | 5.2849 |
Translational Efficiency | 1.0116 | 1.2653 | 0.7831 | 1.0756 | 1.3289 | 1.0214 | 1.414 | 1.3897 | 1.5952 | 0.8373 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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