Standard name
Human Ortholog
Description Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0.05 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.52 0.47 0.55 0.5 0.81 0.79 0.78 0.76 0.5 0.48 0.34 0.31 – 0.35 0.49 – – – 0.33 0.35 0.3 0.23 0.42 0.59
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0.06 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.05 0.17 0 0.11 0 0.13 0.15 0.15 0.52 0.41 0.59 0.5 – 0 0 – – – 0.05 0 0.07 0 0 0
Nucleus 0.2 0.13 0.2 0.28 0 0 0 0.06 0.11 0.18 0.2 0.25 – 0.53 0.37 – – – 0.24 0.24 0.24 0.4 0.17 0.09
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0.1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0.06 0 0 0 0 0 0 0 0 – 0 0 – – – 0.05 0.06 0.08 0.08 0.09 0.08
Vac/Vac Membrane 0.27 0.31 0.17 0.1 0.12 0.09 0 0.07 0 0 0.05 0.08 – 0.09 0.14 – – – 0.24 0.23 0.19 0.09 0.15 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 2 1 0 1 0
Bud 0 0 0 1 2 0 3 0 9 4 13 6 – 0 0 – – – 2 4 2 0 3 2
Bud Neck 1 0 2 2 6 5 7 15 7 11 6 11 – 0 0 – – – 2 4 0 1 1 3
Bud Site 0 0 0 1 0 0 0 0 3 2 5 4 – 0 0 – – – – – – – – –
Cell Periphery 0 0 0 1 0 0 2 0 1 0 0 1 – 0 0 – – – 0 0 0 0 0 0
Cytoplasm 68 134 89 92 277 261 461 398 270 286 185 207 – 31 75 – – – 60 134 83 8 62 87
Endoplasmic Reticulum 0 4 0 0 0 1 0 0 0 0 0 1 – 1 0 – – – 0 1 2 0 1 1
Endosome 2 9 9 7 12 4 9 6 2 7 5 13 – 3 2 – – – 2 5 7 0 2 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 4 0 0 0 0
Mitochondria 7 48 3 21 8 44 89 77 281 243 324 331 – 1 2 – – – 9 11 19 0 5 3
Nucleus 26 36 33 51 15 11 23 29 57 105 112 164 – 47 56 – – – 44 92 65 15 25 13
Nuclear Periphery 1 3 0 0 0 0 0 0 1 2 0 1 – 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 2 0 1 1 – 0 0 – – – 0 1 3 3 3 1
Peroxisomes 0 0 0 0 0 0 0 2 0 0 0 0 – 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 1 4 11 4 1 12 10 3 14 12 28 – 1 0 – – – 9 24 21 3 13 11
Vac/Vac Membrane 36 89 28 18 41 29 25 35 17 14 25 54 – 8 22 – – – 44 87 53 3 22 17
Unique Cell Count 131 283 161 183 340 331 588 521 541 598 547 668 89 152 187 386 276 39 148 148
Labelled Cell Count 141 324 168 205 365 356 631 572 653 688 688 822 92 157 187 386 276 39 148 148


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.8 5.1 4.7 5.1 4.2 4.1 4.3 3.3 3.8 3.6 3.9 – 5.2 4.8 3.6 4.8 4.7 – 5.9 5.7
Std Deviation (1e-4) 0.6 0.8 1.0 1.3 1.6 1.3 1.2 1.2 0.9 1.1 0.9 1.1 – 1.1 1.1 0.5 2.0 0.8 – 2.3 2.1
Intensity Change (Log2) – – – -0.12 -0.01 -0.3 -0.33 -0.27 -0.62 -0.44 -0.51 -0.41 – 0.02 -0.08 -0.49 -0.08 -0.11 – 0.19 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2354 1.5875 1.2881 1.369 0.7742 1.3003 2.0579 2.5567 2.6978 2.2696 2.169 2.4737 -0.492 -0.4683 -0.4834 -0.3587 -0.4341 -0.3677
Actin 0.0372 0.0003 0.0015 0.024 0.0004 0.0002 0.0014 0.0047 0.002 0.0025 0.0028 0.0003 0.0169 0.0004 0.0063 0.0265 0.059 0.0007
Bud 0.0006 0.0001 0.0004 0.0101 0.0001 0.0001 0.0005 0.0002 0.0005 0.0003 0.0025 0 0.0008 0.0002 0.0008 0.0003 0.0006 0.0008
Bud Neck 0.0145 0.0003 0.0007 0.0007 0.0012 0.0026 0.0086 0.0005 0.0011 0.0007 0.0028 0.002 0.0039 0.0006 0.0007 0.0008 0.0016 0.0027
Bud Periphery 0.001 0.0001 0.001 0.0033 0.0001 0.0001 0.0015 0.0002 0.003 0.0007 0.0139 0.0001 0.0008 0.0001 0.0002 0.0005 0.0016 0.0003
Bud Site 0.0106 0.0007 0.0017 0.0109 0.0003 0.0002 0.0017 0.0004 0.0048 0.0009 0.0056 0.0001 0.0133 0.0014 0.0004 0.0005 0.0009 0.0004
Cell Periphery 0.0015 0.0001 0.0005 0.0001 0.0001 0 0.0003 0 0.0027 0.0001 0.0016 0 0.0003 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.0737 0.0828 0.0377 0.0166 0.038 0.0401 0.0312 0.0059 0.0316 0.0479 0.014 0.0214 0.1117 0.1637 0.1197 0.1291 0.0657 0.1472
Cytoplasmic Foci 0.0173 0.0017 0.0093 0.042 0.0013 0.0011 0.003 0.0002 0.0046 0.0058 0.0233 0.0014 0.0101 0.0066 0.0067 0.0019 0.0015 0.0047
Eisosomes 0.0011 0.0001 0.0001 0.0002 0.0005 0 0.0001 0.0001 0.0002 0.0001 0.0013 0 0.0002 0 0.0001 0.0002 0.0001 0
Endoplasmic Reticulum 0.0048 0.003 0.0016 0.0012 0.0016 0.0007 0.0029 0.0016 0.0029 0.0029 0.0024 0.0009 0.0046 0.0032 0.0034 0.0039 0.0051 0.0021
Endosome 0.017 0.0013 0.003 0.0224 0.0022 0.0009 0.0138 0.0014 0.0042 0.016 0.0113 0.0018 0.014 0.0042 0.0058 0.0045 0.0103 0.0045
Golgi 0.0117 0.0001 0.0007 0.0066 0.0001 0 0.0015 0.0002 0.001 0.0061 0.0063 0 0.0051 0.0002 0.0011 0.0027 0.0032 0.0007
Lipid Particles 0.0203 0.0003 0.0026 0.0177 0.0011 0.0001 0.0044 0 0.0071 0.0311 0.0193 0.0002 0.0072 0.0005 0.0061 0.0008 0.0014 0.0011
Mitochondria 0.0105 0.0005 0.0052 0.0029 0.0006 0.0004 0.0052 0.0013 0.0101 0.0328 0.0199 0.0003 0.0027 0.0005 0.0014 0.0023 0.0055 0.0017
None 0.1668 0.1981 0.1515 0.1527 0.2052 0.1285 0.07 0.0379 0.0851 0.006 0.1848 0.064 0.1017 0.1105 0.1335 0.0826 0.0815 0.0756
Nuclear Periphery 0.0147 0.0137 0.0116 0.0164 0.0075 0.0054 0.0353 0.029 0.0139 0.0273 0.005 0.0129 0.021 0.0176 0.0293 0.0093 0.0518 0.0095
Nucleolus 0.007 0.0058 0.0055 0.0104 0.014 0.0116 0.0123 0.0088 0.0101 0.0058 0.0252 0.0175 0.006 0.0036 0.0035 0.0033 0.0056 0.0067
Nucleus 0.5425 0.6699 0.7232 0.6194 0.6895 0.7898 0.762 0.8972 0.7728 0.7974 0.5938 0.826 0.6443 0.6599 0.6456 0.7199 0.6852 0.7084
Peroxisomes 0.0187 0.0001 0.0021 0.0173 0.0002 0 0.0008 0.0001 0.009 0.0063 0.0282 0.0001 0.0032 0.0004 0.0031 0.0002 0.0006 0.0013
Punctate Nuclear 0.0245 0.0199 0.0366 0.0238 0.0343 0.0177 0.0319 0.0096 0.0267 0.0054 0.0204 0.0498 0.0275 0.0235 0.0304 0.0088 0.0152 0.0292
Vacuole 0.0029 0.0008 0.0026 0.0009 0.0012 0.0006 0.0083 0.0004 0.0054 0.002 0.0121 0.0006 0.004 0.0024 0.0014 0.0015 0.002 0.002
Vacuole Periphery 0.0011 0.0002 0.0006 0.0005 0.0002 0.0001 0.0031 0.0004 0.001 0.0017 0.0035 0.0004 0.0008 0.0005 0.0005 0.0004 0.0016 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.2129 4.4431 7.4182 7.3679 4.0276 8.6932 4.2117 7.5803 6.5288 5.2849
Translational Efficiency 1.0116 1.2653 0.7831 1.0756 1.3289 1.0214 1.414 1.3897 1.5952 0.8373

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2494 1156 2055 1337 191 1787 307 1395 2685 2943 2362 2732

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 705.34 699.38 873.70 873.77 650.94 713.82 969.42 832.93 701.47 708.15 886.14 852.92
Standard Deviation 77.27 89.60 114.27 116.28 81.62 80.92 122.04 116.26 78.84 84.73 119.72 118.05
Intensity Change Log 2 — -0.012242 0.308819 0.308935 — 0.133036 0.574597 0.355671 — 0.059311 0.442495 0.331554

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000046 0.000622 0.000335 0.000708 0.000168 0.000151 0.000847 0.000872 0.000055 0.000336 0.000402 0.000792
Bud Neck 0.009676 0.016892 0.020130 0.025491 0.001507 0.019810 0.007090 0.017379 0.009095 0.018664 0.018435 0.021349
Bud Site 0.002049 0.012520 0.003826 0.016113 0.000737 0.004496 0.001095 0.010742 0.001956 0.007648 0.003471 0.013371
Cell Periphery 0.000123 0.000166 0.000089 0.000213 0.000197 0.000134 0.000098 0.000165 0.000128 0.000146 0.000090 0.000188
Cytoplasm 0.430015* 0.377956* 0.015161 0.104946* 0.227600* 0.375086* 0.012649 0.067055 0.415616* 0.376213* 0.014834 0.085598
Cytoplasmic Foci 0.108411 0.179717 0.000065 0.002342 0.099352 0.147859 0.000918 0.001805 0.107766 0.160373 0.000175 0.002068
Eisosomes 0.000074 0.000109 0.000014 0.000032 0.000100 0.000091 0.000046 0.000028 0.000076 0.000098 0.000018 0.000030
Endoplasmic Reticulum 0.000993 0.002090 0.003347 0.007355 0.001874 0.000707 0.007517 0.007295 0.001056 0.001250 0.003889 0.007325
Endosome 0.004830 0.010651 0.000270 0.006094 0.004510 0.007288 0.000457 0.001613 0.004807 0.008609 0.000294 0.003806
Golgi 0.000633 0.002560 0.000048 0.003838 0.000230 0.001585 0.000068 0.000616 0.000604 0.001968 0.000051 0.002193
Lipid Particles 0.009055 0.011716 0.000084 0.000607 0.007547 0.007972 0.000644 0.000596 0.008948 0.009442 0.000157 0.000601
Mitochondria 0.003010 0.011197 0.001800 0.007215 0.000785 0.011158 0.000807 0.003185 0.002852 0.011173 0.001671 0.005157
Mitotic Spindle 0.000966 0.005932 0.004000 0.035376 0.000726 0.002741 0.000608 0.015953 0.000948 0.003995 0.003559 0.025458
None 0.004315 0.010062 0.003195 0.007674 0.010912 0.009179 0.007372 0.009668 0.004785 0.009526 0.003737 0.008692
Nuclear Periphery 0.001373 0.001128 0.001198 0.005481 0.001158 0.001029 0.000790 0.001932 0.001357 0.001068 0.001145 0.003669
Nuclear Periphery Foci 0.000557 0.001581 0.000107 0.001270 0.001865 0.000359 0.000239 0.000754 0.000650 0.000839 0.000124 0.001007
Nucleolus 0.001576 0.004537 0.002388 0.001745 0.005541 0.003866 0.005532 0.002916 0.001858 0.004130 0.002797 0.002343
Nucleus 0.397550* 0.300336* 0.941231* 0.734676* 0.613443* 0.371540* 0.947838* 0.843987* 0.412907* 0.343572* 0.942090* 0.790492*
Peroxisomes 0.002879 0.012893 0.000061 0.000230 0.002134 0.009140 0.000451 0.000366 0.002826 0.010615 0.000112 0.000299
Vacuole 0.021416 0.035704 0.002437 0.034695 0.019279 0.024828 0.004828 0.012622 0.021264 0.029100 0.002748 0.023424
Vacuole Periphery 0.000454 0.001629 0.000213 0.003898 0.000337 0.000980 0.000105 0.000450 0.000445 0.001235 0.000199 0.002137

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.89 -18.04 -14.58 -1.15 -5.94 0.73 -10.80 -8.54 -9.45 1.13 -5.86 -21.22 -16.32 -7.86 -6.34
Bud Neck -4.47 -8.70 -6.34 -1.80 1.49 -11.64 -5.78 -12.34 -0.34 -5.24 -7.35 -8.89 -8.84 -1.26 0.79
Bud Site -5.44 -2.30 -6.18 1.04 -4.09 -5.55 -3.17 -5.96 -3.54 -5.50 -6.22 -2.30 -7.68 -2.13 -5.37
Cell Periphery -2.64 2.36 -1.46 1.32 -4.86 3.06 3.78 2.23 -1.66 -2.89 -1.32 2.74 -1.47 -0.35 -6.60
Cytoplasm 5.74 78.24* 51.85 31.61 -17.10 -7.79 12.38 9.44 42.11 -10.81 5.98 77.53 59.26 52.16 -20.20
Cytoplasmic Foci -10.52 35.66 35.10 30.13 -7.71 -3.74 9.73 9.70 34.01 -0.67 -11.41 36.94 36.38 45.35 -6.00
Eisosomes -3.81 21.57 7.10 6.30 -17.99 1.94 4.62 7.56 13.19 2.51 -4.86 20.07 10.34 12.22 -14.28
Endoplasmic Reticulum -6.15 -13.99 -23.84 -19.51 -13.95 3.77 -9.89 -12.85 -22.60 0.60 -1.79 -17.24 -31.06 -30.60 -14.50
Endosome -5.66 7.92 2.54 7.71 -4.49 -1.90 5.13 3.78 8.40 -4.44 -5.11 8.45 4.27 10.86 -6.34
Golgi -5.39 5.12 -1.02 2.33 -2.21 -4.92 4.17 -0.64 3.66 -1.53 -5.80 5.22 -0.54 3.62 -2.67
Lipid Particles -2.35 14.88 13.45 10.96 -17.27 0.35 5.23 5.37 16.56 0.47 -0.74 15.50 14.33 18.39 -12.56
Mitochondria -5.39 2.84 -2.09 4.30 -3.83 -9.16 -1.98 -5.63 7.01 -5.14 -8.90 3.05 -1.73 7.93 -4.41
Mitotic Spindle -3.78 -4.07 -7.92 -5.59 -6.11 -1.47 -0.18 -6.77 -6.30 -6.67 -4.24 -4.06 -10.49 -8.64 -8.08
None -5.45 2.49 -3.33 3.79 -5.89 0.69 1.73 1.48 1.39 -0.70 -6.00 2.39 -3.53 3.35 -5.75
Nuclear Periphery 1.69 -3.05 -11.03 -11.39 -10.16 1.23 1.03 -4.72 -9.82 -9.81 2.49 -2.57 -13.40 -14.39 -12.13
Nuclear Periphery Foci -3.61 0.93 -3.04 1.63 -5.08 2.50 2.59 0.61 -3.12 -3.29 -1.20 1.56 -2.90 -2.35 -4.67
Nucleolus -7.09 -5.05 -1.71 5.82 4.10 1.81 0.28 1.88 0.19 1.14 -7.62 -5.17 -3.88 3.36 2.55
Nucleus 10.10 -88.31* -34.87 -38.17 24.20 9.31 -14.43 -9.18 -46.70 14.16 9.24 -88.41 -48.72 -58.33 26.98
Peroxisomes -8.63 9.22 8.94 11.49 -4.66 -8.20 3.05 4.25 12.71 1.08 -11.64 9.38 9.07 17.14 -1.62
Vacuole -6.55 14.48 -14.09 -10.38 -18.04 -0.18 2.82 -1.62 -2.85 -7.96 -5.12 14.36 -13.54 -10.72 -20.49
Vacuole Periphery -8.01 3.77 -2.42 0.77 -3.14 -4.56 4.21 0.17 4.59 -3.64 -7.65 4.25 -2.05 2.05 -3.48
Standard name
Human Ortholog
Description Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (17%), cytoplasm (11%), mixed (45%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cin5

Cin5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cin5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available