Standard name
Human Ortholog
Description Ubiquitin-binding protein; functions as ubiquitin-Atg8p adaptor in ubiquitin-dependent autophagy; serves as proteaphagy receptor for inactivated 26S proteasomes; contains CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; CUE5 has a paralog, DON1, that arose from the whole genome duplication; human TOLLIP is a functional CUE-domain homolog, can complement yeast null mutant, rescuing hypersensitivity of cue5 null mutant cells to Htt-96Q
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.08 0.05 0.21 0.12 0.1 0.12 0.1 0.07 0.11 0.13 0.09 0.1 0.07 0.09 0 0 0 0.07 0 0 0.1 0.07 0.08 0.12
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.1 0 0 0 0.05 0.06 0.1 0.08 0.05 0.06 0 0.07 0.07 0 0.08 0 0 0 0 0 0 0
Cytoplasm 0.78 0.87 0.44 0.54 0.62 0.49 0.41 0.4 0.33 0.32 0.31 0.31 0.7 0.72 0.85 0.88 0.79 0.78 0.52 0.56 0.3 0.31 0.31 0.26
Endoplasmic Reticulum 0.08 0.12 0.28 0.23 0.22 0.23 0.22 0.2 0.25 0.26 0.13 0.16 0.27 0.23 0.12 0 0.05 0.06 0.15 0.17 0.22 0.18 0.28 0.29
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0
Golgi 0 0 0.19 0.21 0.07 0.09 0.07 0.05 0.09 0 0.05 0 0 0.06 0 0.08 0.1 0.05 0.13 0.1 0.24 0.13 0.19 0.19
Mitochondria 0.12 0 0 0.12 0.1 0.34 0.45 0.46 0.42 0.49 0.59 0.56 0 0 0 0 0 0 0 0 0.06 0.06 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0 0.09 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 6 55 17 23 35 41 23 25 47 24 31 9 9 5 4 8 10 3 5 31 4 9 12
Bud 0 0 2 2 6 4 8 8 1 12 6 10 0 1 1 0 1 0 1 1 0 0 3 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 26 4 5 11 20 19 23 29 14 19 3 7 10 6 14 6 0 0 10 0 0 0
Cytoplasm 71 110 117 76 150 142 162 130 74 119 82 96 93 71 129 148 145 113 53 73 93 21 36 29
Endoplasmic Reticulum 7 15 75 32 52 68 87 64 57 96 33 50 36 23 18 6 9 9 15 22 70 12 32 32
Endosome 1 0 1 0 3 2 0 2 3 0 1 2 0 0 0 0 2 2 1 2 8 5 3 3
Golgi 2 1 50 29 18 26 26 16 21 12 13 13 2 6 6 14 19 7 13 13 75 8 21 21
Mitochondria 11 3 12 17 25 98 179 150 96 180 154 174 0 0 0 0 8 1 3 2 19 3 3 4
Nucleus 1 1 0 0 2 0 0 1 0 0 2 0 0 0 1 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 3 0 0 0 0 1 0 1 0 0 0 0 0
Nucleolus 2 1 1 0 0 0 2 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0
Vac/Vac Membrane 0 0 3 1 1 1 5 9 6 10 4 10 1 1 0 3 7 12 2 6 1 6 4 4
Unique Cell Count 91 127 268 140 240 290 399 326 226 367 262 313 132 99 151 169 184 144 102 131 315 70 116 112
Labelled Cell Count 103 138 343 178 285 387 531 422 306 505 333 409 144 118 170 181 215 160 102 131 315 70 116 112


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.5 15.1 13.6 12.5 12.6 11.9 11.2 11.4 11.5 11.0 10.0 10.5 15.4 15.3 15.1 25.6 22.5 22.8 – 16.4 16.1
Std Deviation (1e-4) 2.5 2.7 1.8 2.0 1.8 1.8 2.1 2.2 2.4 2.4 2.5 2.5 2.4 2.3 2.5 7.2 6.5 6.7 – 2.3 2.9
Intensity Change (Log2) – – – -0.12 -0.11 -0.19 -0.27 -0.25 -0.23 -0.3 -0.43 -0.37 0.18 0.17 0.15 0.92 0.73 0.75 – 0.28 0.25

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000102030WT3HU80HU120HU1600102030WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30102030WT1AF140AF1800102030
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.1 -3.4 -2.7 -3.7 -4.8 -2.8 -2.6 -3.7 -3.6 -3.5 -2.6 -4.8 -5.4 -4.9 -3.6
Bud 0 0 0 0 0 0 2.1 0 2.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.5 -3.6 -2.8 -2.3 -1.8 0.2 -0.8 -1.9 -1.6 -2.7 -0.8 -1.1 -2.4 -0.8 -2.0
Cytoplasm 2.0 4.2 1.3 -0.8 -0.9 -2.5 -2.9 -2.9 -3.2 5.0 4.8 8.3 9.2 7.4 6.8
Endoplasmic Reticulum -1.1 -1.6 -1.2 -1.8 -2.4 -0.7 -0.5 -4.4 -3.5 -0.1 -0.9 -3.8 -6.4 -6.2 -5.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.5 -3.7 -3.3 -4.8 -5.3 -3.0 -6.5 -4.9 -5.6 -4.8 -3.0 -4.2 -3.0 -2.4 -3.9
Mitochondria 2.9 2.6 8.7 11.3 11.3 10.2 12.1 13.5 13.2 0 0 0 0 -0.1 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 1.4 0 1.4 0 1.7 0 0 0 0 0 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.4555 11.2564 10.343 9.5178 8.7185 10.283 8.5897 12.084 11.0527 10.0467 9.6408 10.7956 9.0941 11.9135 10.2953 9.5871 8.8725 10.3226
Actin 0.0623 0.0195 0.0431 0.1134 0.0665 0.0415 0.0465 0.0137 0.0243 0.0143 0.0792 0.0723 0.0325 0.0299 0.0665 0.0359 0.1409 0.0525
Bud 0.0024 0.0026 0.0025 0.0036 0.0023 0.0017 0.0012 0.002 0.0021 0.0014 0.0016 0.0014 0.0022 0.003 0.0047 0.006 0.0013 0.001
Bud Neck 0.0018 0.0009 0.0015 0.0014 0.0026 0.0096 0.0016 0.0007 0.0022 0.0024 0.0015 0.0205 0.0016 0.0008 0.0032 0.0024 0.0021 0.0105
Bud Periphery 0.0027 0.0013 0.0014 0.0043 0.0023 0.0021 0.0009 0.0007 0.0014 0.0011 0.0011 0.0025 0.0024 0.0009 0.0021 0.0037 0.0007 0.0009
Bud Site 0.003 0.0065 0.0026 0.0033 0.002 0.0008 0.004 0.0062 0.0073 0.0011 0.0008 0.0011 0.0021 0.0066 0.0087 0.0008 0.0014 0.0005
Cell Periphery 0.0018 0.0004 0.0006 0.001 0.0008 0.0008 0.002 0.0005 0.0007 0.0005 0.0004 0.0016 0.0011 0.0007 0.0007 0.0006 0.0004 0.0005
Cytoplasm 0.2766 0.3666 0.3566 0.2642 0.1678 0.1954 0.3399 0.5838 0.5287 0.4296 0.3508 0.3157 0.3439 0.4867 0.4012 0.3599 0.1035 0.202
Cytoplasmic Foci 0.0717 0.0987 0.0756 0.0579 0.1013 0.0746 0.0765 0.0692 0.0804 0.1399 0.0869 0.07 0.0708 0.079 0.0829 0.0908 0.0821 0.0676
Eisosomes 0.0004 0.0001 0.0001 0.0014 0.0004 0.0005 0.0005 0 0.0001 0.0001 0.0001 0.0007 0.0003 0.0001 0.0005 0.0002 0.0002 0.0002
Endoplasmic Reticulum 0.0427 0.0111 0.0133 0.0069 0.0132 0.0182 0.0241 0.0132 0.0254 0.0226 0.0156 0.0329 0.0646 0.0113 0.0168 0.0129 0.0111 0.0408
Endosome 0.2034 0.2245 0.2653 0.2635 0.2446 0.222 0.2519 0.1913 0.1927 0.23 0.1772 0.1838 0.2054 0.1911 0.2155 0.3267 0.1711 0.2393
Golgi 0.2713 0.2446 0.2033 0.2285 0.3538 0.3763 0.168 0.1033 0.0922 0.1255 0.1813 0.2464 0.2062 0.1748 0.1457 0.1154 0.4466 0.3408
Lipid Particles 0.0011 0.0006 0.0022 0.0006 0.0007 0.0005 0.0192 0.0016 0.0039 0.0013 0.0036 0.0026 0.0042 0.0004 0.0073 0.0058 0.0037 0.0016
Mitochondria 0.0435 0.0134 0.0183 0.0315 0.0299 0.0477 0.026 0.0043 0.0142 0.006 0.056 0.0388 0.0266 0.0072 0.02 0.006 0.0102 0.0291
None 0.0012 0.0006 0.0007 0.0005 0.0015 0.0005 0.0053 0.0005 0.0021 0.0006 0.0006 0.0009 0.0023 0.0008 0.0017 0.0009 0.0003 0.0008
Nuclear Periphery 0.002 0.0006 0.0007 0.0008 0.0009 0.0005 0.0058 0.0007 0.0013 0.0008 0.0005 0.001 0.0103 0.0005 0.0028 0.0025 0.0003 0.0039
Nucleolus 0 0 0 0 0 0 0.0004 0 0.0001 0 0 0.0001 0.0002 0.0001 0.0002 0.0001 0 0.0001
Nucleus 0.0006 0.0004 0.0004 0.0004 0.0004 0.0003 0.0056 0.0004 0.0007 0.0005 0.0003 0.0005 0.0039 0.0005 0.001 0.0006 0.0001 0.0005
Peroxisomes 0.0006 0.0013 0.0014 0.0008 0.001 0.0007 0.009 0.0007 0.003 0.0052 0.0328 0.0021 0.0015 0.0006 0.0042 0.0017 0.0202 0.0011
Punctate Nuclear 0.0002 0.0001 0.0003 0.0002 0.0001 0.0001 0.0028 0.0001 0.0046 0.0079 0.0033 0.0003 0.0017 0.0002 0.001 0.001 0.0005 0.0011
Vacuole 0.0068 0.0038 0.0063 0.0073 0.0045 0.0032 0.0055 0.0048 0.0082 0.0059 0.0033 0.0026 0.0128 0.0031 0.0094 0.0192 0.0017 0.0024
Vacuole Periphery 0.004 0.0023 0.0038 0.0085 0.0033 0.0032 0.0035 0.0021 0.0045 0.0033 0.003 0.0025 0.0036 0.0019 0.0038 0.0067 0.0014 0.0029

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 56.2415 35.9118 55.6649 67.3306 59.0301 47.2881 55.0707 67.2582 74.1963 58.0455
Translational Efficiency 1.7573 2.3429 1.756 1.7313 1.6317 2.2017 1.4368 1.6927 1.6778 1.5335

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1120 314 2664 1549 1957 1422 145 1443 3077 1736 2809 2992

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1138.54 1424.87 1531.11 1574.02 1235.20 1302.95 1661.98 1548.16 1200.02 1325.00 1537.87 1561.55
Standard Deviation 192.35 229.88 213.40 276.63 193.41 209.61 236.77 259.30 198.55 218.52 216.62 268.72
Intensity Change Log 2 — 0.323645 0.427393 0.467269 — 0.077037 0.428158 0.325810 — 0.200587 0.427791 0.395392

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.009416 0.011650 0.024349 0.045936 0.007514 0.006791 0.043560 0.025068 0.008207 0.007670 0.025340 0.035872
Bud Neck 0.000291 0.000565 0.000656 0.004792 0.000313 0.000510 0.006281 0.003350 0.000305 0.000520 0.000946 0.004097
Bud Site 0.025602 0.046782 0.045021 0.185659 0.020136 0.033739 0.087443 0.131445 0.022125 0.036099 0.047210 0.159512
Cell Periphery 0.003246 0.006216 0.003910 0.054715 0.001427 0.001395 0.009781 0.013106 0.002089 0.002267 0.004213 0.034647
Cytoplasm 0.025148 0.046486 0.069307 0.290700 0.025949 0.045179 0.049516 0.227964 0.025657 0.045416 0.068285 0.260443
Cytoplasmic Foci 0.507747 0.430925 0.272760 0.093779 0.439978 0.484795 0.156361 0.156855 0.464645 0.475051 0.266751 0.124200
Eisosomes 0.000301 0.000217 0.000485 0.000684 0.000164 0.000149 0.000404 0.000257 0.000214 0.000161 0.000481 0.000478
Endoplasmic Reticulum 0.019536 0.030639 0.019212 0.024284 0.021962 0.013864 0.063310 0.009957 0.021079 0.016898 0.021488 0.017375
Endosome 0.066098 0.061208 0.060219 0.030455 0.056417 0.056415 0.192327 0.063484 0.059941 0.057282 0.067038 0.046384
Golgi 0.299710 0.313781 0.420468 0.128843 0.377133 0.300313 0.220735 0.226485 0.348952 0.302749 0.410158 0.175934
Lipid Particles 0.002691 0.011862 0.019582 0.084259 0.002867 0.002487 0.019800 0.041853 0.002803 0.004183 0.019594 0.063807
Mitochondria 0.005850 0.001909 0.007373 0.004051 0.007525 0.004412 0.000806 0.002567 0.006915 0.003959 0.007034 0.003336
Mitotic Spindle 0.000357 0.000750 0.003116 0.001980 0.001109 0.002077 0.007853 0.008142 0.000835 0.001837 0.003361 0.004952
None 0.000916 0.000079 0.000172 0.000340 0.000182 0.000144 0.000050 0.000250 0.000449 0.000132 0.000166 0.000297
Nuclear Periphery 0.000027 0.000053 0.000054 0.000113 0.000029 0.000030 0.000326 0.000060 0.000029 0.000034 0.000068 0.000087
Nuclear Periphery Foci 0.000604 0.000553 0.000908 0.001722 0.000485 0.000549 0.007051 0.001965 0.000528 0.000550 0.001225 0.001839
Nucleolus 0.000207 0.000006 0.000010 0.000049 0.000012 0.000014 0.000028 0.000045 0.000083 0.000012 0.000011 0.000047
Nucleus 0.000118 0.000079 0.000140 0.000988 0.000070 0.001291 0.000423 0.000968 0.000088 0.001072 0.000154 0.000978
Peroxisomes 0.002144 0.001799 0.003813 0.006808 0.000861 0.001092 0.003536 0.004029 0.001328 0.001220 0.003799 0.005468
Vacuole 0.005112 0.011051 0.022987 0.026082 0.008185 0.014321 0.111874 0.061905 0.007067 0.013730 0.027575 0.043359
Vacuole Periphery 0.024880 0.023391 0.025459 0.013760 0.027683 0.030434 0.018535 0.020245 0.026663 0.029160 0.025102 0.016888

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.56 -6.38 -11.64 -7.53 -6.75 0.45 -3.48 -7.30 -7.31 1.79 0.36 -8.97 -14.12 -13.23 -4.20
Bud Neck -2.85 -6.62 -17.85 -15.94 -16.26 -1.57 -6.22 -15.94 -14.02 2.96 -2.22 -7.67 -23.37 -20.26 -18.57
Bud Site -4.03 -7.32 -28.90 -19.64 -25.71 -5.10 -6.15 -22.90 -18.60 -3.60 -5.88 -12.06 -37.11 -29.97 -28.22
Cell Periphery -2.34 -1.35 -16.55 -14.63 -16.43 0.11 -2.72 -8.30 -8.26 -0.90 -0.52 -6.13 -18.25 -18.00 -16.90
Cytoplasm -3.53 -13.39 -33.00 -25.42 -26.97 -6.34 -2.29 -26.85 -23.64 -13.99 -7.43 -15.04 -42.37 -36.73 -32.39
Cytoplasmic Foci 5.58 30.16 54.71 27.39 36.77 -5.53 21.82 42.56 45.83 0.10 -1.50 35.45 68.21 58.85 32.48
Eisosomes 1.31 -3.50 -6.03 -6.50 -3.21 0.58 -2.68 -2.26 -2.43 1.62 2.00 -7.11 -7.36 -8.42 0.23
Endoplasmic Reticulum -2.18 0.13 -1.89 1.30 -2.31 4.06 -3.66 7.12 2.61 4.79 2.33 -0.26 2.71 -0.10 2.82
Endosome 1.25 2.37 14.69 9.05 19.12 0.00 -10.45 -2.25 -2.24 9.96 1.41 -3.82 8.38 6.44 12.00
Golgi -1.09 -15.27 22.29 15.21 43.94 9.76 8.44 18.81 9.53 -0.03 7.02 -9.55 30.63 20.36 38.49
Lipid Particles -4.23 -14.06 -21.95 -16.90 -16.64 0.74 -5.73 -15.24 -15.27 -5.60 -2.39 -15.03 -26.35 -25.21 -17.26
Mitochondria 4.85 -1.60 2.29 -3.44 4.41 3.23 8.33 6.87 2.42 -2.78 3.87 -0.12 6.57 1.06 5.68
Mitotic Spindle -2.55 -5.73 -10.44 -5.92 2.28 -1.38 -2.90 -5.66 -4.39 -0.28 -1.74 -5.28 -6.69 -3.90 -2.24
None 1.03 0.92 0.71 -14.02 -9.46 0.54 6.11 -1.08 -1.19 -2.75 1.05 0.96 0.48 -2.57 -3.60
Nuclear Periphery -0.83 -2.62 -3.40 -1.61 -2.33 -0.18 -2.49 -7.88 -4.79 2.20 -0.75 -4.25 -4.71 -3.82 -1.29
Nuclear Periphery Foci 0.15 -0.91 -3.18 -5.60 -5.22 -0.25 -3.28 -5.01 -4.00 2.47 -0.08 -3.65 -6.55 -5.38 -3.08
Nucleolus 1.01 1.00 0.81 -12.00 -11.47 0.00 -0.91 -3.01 -5.20 -1.64 0.99 1.02 0.52 -7.73 -12.72
Nucleus 0.80 -0.43 -11.36 -14.52 -13.65 -1.50 -3.04 -4.45 0.01 -2.77 -1.48 -3.21 -7.94 -0.16 -7.51
Peroxisomes 0.81 -3.56 -7.58 -8.59 -4.93 -1.55 -3.88 -11.38 -10.18 -0.61 0.64 -7.42 -12.78 -13.60 -3.82
Vacuole -3.53 -12.89 -13.05 -7.12 -1.85 -4.38 -6.50 -17.18 -14.73 2.90 -5.81 -12.96 -20.53 -15.30 -7.54
Vacuole Periphery 0.48 -0.27 5.30 3.82 9.20 -1.23 2.82 4.28 5.33 -0.42 -1.34 1.02 7.37 7.90 7.29
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Ubiquitin-binding protein; functions as ubiquitin-Atg8p adaptor in ubiquitin-dependent autophagy; serves as proteaphagy receptor for inactivated 26S proteasomes; contains CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; CUE5 has a paralog, DON1, that arose from the whole genome duplication; human TOLLIP is a functional CUE-domain homolog, can complement yeast null mutant, rescuing hypersensitivity of cue5 null mutant cells to Htt-96Q
Localization
Cell Percentages cytoplasm (32%), Golgi (0%), mixed (44%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cue5

Cue5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cue5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available