Standard name
Human Ortholog
Description Component of the spindle checkpoint; involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability; recruits condensin to the pericentric region of chromosomes during meiosis; dissociates from pericentromeres when sister kinetochores are under tension
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0.05 0.08 0.05 0.05 – 0 0.06 0 0.05 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.77 0.82 0.79 0.78 0.67 0.81 0.79 – 0.67 0.71 0.52 0.63 – – – 0.7 0.66 0.72 0.46 0.57 0.37 0.39 0.79 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0.08 0.06 0.07 0 0 0 0 0 0
Endosome 0 0.07 0 0 0 0 0 – 0 0 0 0 – – – 0.11 0.13 0.1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0 0 0 0 0 0.07 – 0.28 0.19 0.53 0.38 – – – 0 0 0 0.15 0 0.15 0 0.05 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0.05 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0.06 0.09 0.15 0.17 0.25 0.14 0.14 – 0.11 0.14 0.06 0.08 – – – 0.17 0.16 0.12 0.23 0.21 0.3 0.59 0 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0.07 0.06 0.05 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 – 0 1 0 0 – – – 1 0 0 0 2 0 0 0 0
Bud 0 0 0 0 2 0 2 – 3 2 3 6 – – – 0 0 1 0 6 1 0 0 1
Bud Neck 1 2 0 6 18 9 11 – 6 12 5 8 – – – 2 2 2 0 2 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 1 0 1 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 1 – 2 1 0 1 – – – 2 1 2 0 0 0 0 0 0
Cytoplasm 79 134 31 95 155 158 166 – 112 141 74 102 – – – 151 115 138 52 109 23 1 13 38
Endoplasmic Reticulum 2 0 0 1 2 0 0 – 0 0 0 1 – – – 17 11 14 0 0 1 0 0 0
Endosome 3 11 1 2 6 5 0 – 2 1 1 2 – – – 23 22 19 2 6 1 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 2 1 0 0 0 0 0 0
Mitochondria 18 1 1 2 1 6 15 – 47 38 76 61 – – – 6 3 8 16 6 9 0 0 1
Nucleus 0 4 0 2 5 1 6 – 5 4 3 5 – – – 7 2 4 0 5 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 3 1 1 1 – – – 5 9 1 0 4 0 0 0 0
Peroxisomes 0 1 0 0 0 1 0 – 0 0 0 0 – – – 1 2 0 0 0 0 0 0 0
SpindlePole 6 14 6 21 58 27 29 – 18 29 9 13 – – – 36 28 22 26 39 18 2 0 2
Vac/Vac Membrane 1 3 1 0 4 2 0 – 2 3 4 2 – – – 9 13 12 5 3 2 0 0 0
Unique Cell Count 102 164 39 122 232 196 210 167 200 143 162 216 174 191 113 191 63 5 17 49
Labelled Cell Count 110 170 40 130 251 210 230 200 234 176 203 260 210 224 113 191 63 5 17 49


Nucleus, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.3 4.6 4.5 5.1 4.2 4.1 – 3.5 3.7 3.2 3.6 – – – 5.7 5.7 5.3 – – 4.6
Std Deviation (1e-4) 0.4 1.1 0.6 1.2 1.6 1.4 1.5 – 1.2 1.3 1.0 1.3 – – – 1.3 1.4 1.2 – – 1.5
Intensity Change (Log2) – – – -0.02 0.16 -0.13 -0.15 – -0.39 -0.31 -0.5 -0.34 – – – 0.33 0.32 0.22 – – 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.9173 -0.7708 -0.4192 -0.4671 -0.3494 -0.5899 -0.3945 -0.3386 -0.1742 0.2532 -0.0316 -0.6615 -1.3692 -1.1862 -1.0037 -0.4135 -0.5162 -1.2702
Actin 0.0221 0.0033 0.0098 0.0016 0.0036 0.0097 0.024 0.0004 0.0015 0.056 0.029 0.0026 0.0294 0.002 0.0148 0.12 0.026 0.0047
Bud 0.0018 0.0032 0.0058 0 0.0004 0.003 0.0008 0.0004 0.0005 0.0002 0.0027 0.0001 0.0036 0.0113 0.0004 0.0012 0.0013 0
Bud Neck 0.0062 0.0037 0.0094 0.0006 0.0006 0.0034 0.0074 0.0008 0.001 0.0042 0.0014 0.001 0.0014 0.0011 0.0012 0.0047 0.0025 0.0003
Bud Periphery 0.0035 0.0012 0.0059 0.0001 0.0008 0.0041 0.0015 0.0004 0.0019 0.0003 0.0142 0.0003 0.0012 0.0032 0.0004 0.0018 0.0035 0.0001
Bud Site 0.0153 0.005 0.0047 0.0001 0.0012 0.0011 0.014 0.0076 0.002 0.0011 0.0045 0.0003 0.003 0.0128 0.0019 0.0061 0.0016 0
Cell Periphery 0.0006 0.0002 0.0002 0 0.0002 0.0002 0.0007 0.0001 0.0002 0.0002 0.0006 0.0001 0.0003 0.0006 0.0001 0.0003 0.0003 0
Cytoplasm 0.0292 0.031 0.0368 0.0482 0.0269 0.0455 0.0291 0.021 0.028 0.0508 0.06 0.0322 0.048 0.058 0.0531 0.1248 0.0761 0.0746
Cytoplasmic Foci 0.0741 0.0376 0.0851 0.0065 0.03 0.0192 0.0888 0.046 0.0794 0.0408 0.0405 0.0354 0.1211 0.0664 0.1531 0.0206 0.0187 0.0207
Eisosomes 0.0009 0.0002 0.0003 0.0001 0.0003 0.0003 0.0009 0.0001 0.0001 0.0002 0.0003 0.0001 0.0005 0.0002 0.0002 0.0004 0.0003 0.0001
Endoplasmic Reticulum 0.0043 0.0004 0.001 0.0012 0.0074 0.0035 0.0028 0.0004 0.0017 0.0041 0.002 0.0007 0.0028 0.0007 0.0027 0.0023 0.0101 0.0004
Endosome 0.0103 0.0028 0.0141 0.0067 0.0418 0.0049 0.0227 0.0011 0.0104 0.0187 0.0484 0.0038 0.0137 0.0028 0.0153 0.0093 0.0656 0.0031
Golgi 0.0046 0.0004 0.0088 0.0006 0.0199 0.0035 0.005 0.0004 0.0035 0.0276 0.017 0.0014 0.0035 0.0005 0.0045 0.0058 0.0151 0.0005
Lipid Particles 0.0125 0.0053 0.0118 0.0038 0.0083 0.0063 0.0185 0.0031 0.0209 0.0424 0.0492 0.0054 0.0207 0.0049 0.0156 0.0163 0.0955 0.0016
Mitochondria 0.0032 0.0005 0.0057 0.0009 0.008 0.0059 0.0128 0.0067 0.0146 0.0074 0.0107 0.0041 0.0052 0.0006 0.0044 0.0038 0.0161 0.0002
None 0.7101 0.7212 0.34 0.5032 0.7801 0.7886 0.6316 0.7721 0.3479 0.3936 0.6089 0.8593 0.6179 0.7157 0.3353 0.5505 0.5675 0.8538
Nuclear Periphery 0.0077 0.0023 0.0042 0.0184 0.0141 0.0073 0.0094 0.0011 0.0056 0.0103 0.0057 0.0031 0.0169 0.0009 0.0061 0.01 0.0329 0.0021
Nucleolus 0.006 0.0118 0.0089 0.0081 0.0006 0.008 0.0044 0.0066 0.0075 0.0046 0.0029 0.0009 0.0046 0.0091 0.0067 0.0015 0.002 0.0002
Nucleus 0.0119 0.0549 0.088 0.2896 0.0024 0.025 0.0163 0.025 0.0636 0.1788 0.0035 0.006 0.0049 0.0235 0.0555 0.0823 0.0078 0.0011
Peroxisomes 0.0133 0.0035 0.0148 0.0008 0.0298 0.0065 0.0128 0.0055 0.0139 0.0322 0.026 0.0054 0.0209 0.0022 0.0115 0.0162 0.0357 0.0015
Punctate Nuclear 0.0582 0.1104 0.3417 0.1085 0.017 0.0512 0.0915 0.1004 0.3938 0.1246 0.0592 0.0372 0.0777 0.081 0.3159 0.0197 0.0105 0.0346
Vacuole 0.003 0.0011 0.0021 0.0008 0.0031 0.0019 0.0035 0.0005 0.0014 0.0011 0.0092 0.0003 0.0019 0.0023 0.0011 0.0017 0.0059 0.0001
Vacuole Periphery 0.0011 0.0001 0.0007 0.0004 0.0034 0.001 0.0016 0.0001 0.0005 0.0007 0.0041 0.0002 0.0009 0.0002 0.0004 0.0006 0.005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.0691 20.5843 10.7334 7.576 16.1378 13.6838 18.1408 14.8065 7.7289 17.3043
Translational Efficiency 0.7841 0.6194 0.3822 0.5214 0.4615 0.556 0.461 0.4687 0.6224 0.4692

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1726 1269 1363 1341 1314 1269 1878 1927 3040 2538 3241 3268

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 576.50 648.26 875.01 799.01 668.67 689.11 834.76 847.15 616.34 668.68 851.69 827.40
Standard Deviation 70.29 114.19 105.69 109.43 73.34 84.31 93.75 108.79 84.94 102.42 100.92 111.59
Intensity Change Log 2 — 0.169252 0.601979 0.470893 — 0.043440 0.320067 0.341323 — 0.103056 0.457460 0.402762

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000109 0.000430 0.000306 0.000859 0.000047 0.000363 0.000156 0.001945 0.000083 0.000396 0.000219 0.001499
Bud Neck 0.027806 0.044227 0.006887 0.012777 0.008310 0.048434 0.004391 0.021633 0.019379 0.046330 0.005441 0.017999
Bud Site 0.012146 0.015965 0.010034 0.017947 0.001821 0.018284 0.004226 0.032025 0.007683 0.017124 0.006669 0.026248
Cell Periphery 0.000659 0.000212 0.000132 0.000274 0.000174 0.000181 0.000131 0.000205 0.000449 0.000197 0.000132 0.000233
Cytoplasm 0.455266* 0.304053* 0.342267* 0.173088* 0.507433* 0.327515* 0.334976* 0.155129* 0.477814* 0.315784* 0.338042* 0.162498*
Cytoplasmic Foci 0.206625* 0.286251* 0.008923 0.015170 0.193811* 0.256792* 0.007438 0.009894 0.201087* 0.271522* 0.008063 0.012059
Eisosomes 0.000358 0.000185 0.000036 0.000047 0.000110 0.000139 0.000033 0.000033 0.000251 0.000162 0.000034 0.000038
Endoplasmic Reticulum 0.001770 0.000944 0.003713 0.003775 0.000406 0.000720 0.001900 0.003576 0.001181 0.000832 0.002662 0.003658
Endosome 0.007158 0.045924 0.002613 0.007006 0.005993 0.034037 0.001615 0.006908 0.006654 0.039980 0.002035 0.006949
Golgi 0.002207 0.010241 0.000116 0.002438 0.001094 0.007609 0.000068 0.009581 0.001726 0.008925 0.000088 0.006650
Lipid Particles 0.015115 0.019194 0.001491 0.002031 0.009953 0.013033 0.001052 0.001392 0.012884 0.016113 0.001237 0.001654
Mitochondria 0.007342 0.026490 0.001384 0.005892 0.003492 0.020374 0.001411 0.009499 0.005678 0.023432 0.001400 0.008019
Mitotic Spindle 0.001683 0.025018 0.011958 0.154991* 0.001991 0.023694 0.010361 0.220914* 0.001816 0.024356 0.011033 0.193863*
None 0.039799 0.011663 0.006643 0.014543 0.011781 0.006882 0.009054 0.010541 0.027689 0.009273 0.008040 0.012184
Nuclear Periphery 0.000645 0.001278 0.001827 0.002865 0.000595 0.000843 0.002061 0.003404 0.000623 0.001060 0.001962 0.003183
Nuclear Periphery Foci 0.001531 0.002378 0.001813 0.003503 0.000760 0.000881 0.000632 0.002871 0.001198 0.001629 0.001129 0.003131
Nucleolus 0.016254 0.013907 0.001899 0.012354 0.004230 0.008346 0.001357 0.007830 0.011056 0.011126 0.001585 0.009686
Nucleus 0.178723* 0.108424 0.562419* 0.526011* 0.227165* 0.132028* 0.593433* 0.472526* 0.199661* 0.120226 0.580390* 0.494474*
Peroxisomes 0.004333 0.028236 0.000371 0.003092 0.004353 0.022215 0.000262 0.002145 0.004342 0.025226 0.000308 0.002534
Vacuole 0.018975 0.050983 0.034883 0.037090 0.016053 0.074012 0.025236 0.025577 0.017712 0.062498 0.029293 0.030301
Vacuole Periphery 0.001495 0.003997 0.000286 0.004246 0.000428 0.003619 0.000206 0.002372 0.001034 0.003808 0.000239 0.003141

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.00 -13.13 -13.34 -7.92 -5.64 -2.23 -11.57 -5.85 -3.76 -3.65 -4.36 -16.83 -9.16 -5.89 -5.13
Bud Neck -5.17 6.26 1.24 6.29 -5.59 -12.44 -1.00 -12.04 5.56 -11.87 -11.68 5.97 -5.64 8.09 -12.72
Bud Site -2.32 -1.99 -5.66 -3.58 -3.17 -7.75 -6.37 -12.19 -2.33 -8.61 -6.62 -3.09 -11.24 -4.09 -8.36
Cell Periphery 7.21 8.47 7.34 -0.09 -2.71 -0.11 1.40 1.81 2.66 0.88 6.93 8.54 7.95 1.75 -1.45
Cytoplasm 11.60 13.94 35.44 19.12 18.56 14.62 21.34 43.34 25.03 24.24 18.05 24.65 56.00 31.51 30.48
Cytoplasmic Foci -4.96 36.81 35.44 39.39 -1.95 -5.01 32.47 32.93 38.70 2.94 -6.80 49.02 48.73 55.50 0.33
Eisosomes 4.78 8.92 8.09 7.76 -4.19 -2.80 8.34 11.46 13.41 2.82 4.38 10.02 9.88 13.72 -0.85
Endoplasmic Reticulum 2.08 -11.48 -12.53 -17.87 -1.64 -3.44 -19.36 -21.83 -19.79 -4.62 1.40 -17.17 -20.26 -26.50 -4.32
Endosome -15.14 3.88 0.63 15.20 -2.68 -13.06 5.38 3.30 14.77 -3.20 -19.96 7.16 3.29 21.25 -4.04
Golgi -9.33 8.11 2.34 10.35 -5.16 -6.95 6.73 -2.10 4.05 -3.55 -11.25 10.34 -0.88 8.76 -4.60
Lipid Particles -2.51 13.28 12.96 11.90 -0.98 -1.90 9.43 9.81 11.99 2.54 -2.88 16.39 16.53 16.76 1.07
Mitochondria -7.31 5.28 2.83 8.95 -2.64 -8.42 2.28 -0.92 8.38 -4.69 -10.75 5.73 1.67 12.16 -5.27
Mitotic Spindle -8.67 -8.14 -23.73 -19.25 -19.35 -7.44 -8.09 -31.68* -27.02* -27.42* -11.44 -11.53 -39.56 -33.01* -33.44*
None 9.73 13.60 12.11 2.31 -2.55 3.60 3.78 1.76 -3.06 -3.63 10.66 13.70 11.89 0.37 -4.43
Nuclear Periphery -3.11 -17.89 -15.66 -10.78 -8.02 -2.05 -22.84 -17.81 -15.58 -7.63 -3.69 -27.99 -23.40 -18.36 -10.83
Nuclear Periphery Foci 1.39 -2.60 -6.38 -7.68 -3.81 0.64 -3.46 -7.33 -8.10 -3.74 1.76 -3.54 -8.82 -10.83 -5.29
Nucleolus 4.55 8.80 -1.24 -5.63 -9.57 -1.93 2.76 -11.20 -9.39 -13.45 3.42 9.54 -7.03 -10.66 -16.46
Nucleus 8.50 -41.87 -24.93 -29.67 8.89 10.93 -41.54 -20.72 -30.45 14.96 13.27 -61.05 -33.33 -42.83 17.13
Peroxisomes -14.12 12.35 3.24 14.90 -4.70 -9.76 8.65 6.52 12.75 -3.87 -16.92 14.62 7.85 19.77 -6.05
Vacuole -11.54 -12.17 -10.05 -0.99 0.43 -14.70 -12.59 -9.34 6.81 2.10 -18.46 -17.12 -13.40 4.68 1.87
Vacuole Periphery -5.77 3.49 -0.28 3.66 -2.24 -8.81 3.93 -1.44 6.56 -2.31 -9.45 4.06 -0.39 7.40 -3.11
Standard name
Human Ortholog
Description Component of the spindle checkpoint; involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability; recruits condensin to the pericentric region of chromosomes during meiosis; dissociates from pericentromeres when sister kinetochores are under tension
Localization
Cell Percentages cytoplasm (17%), nucleus (8%), mixed (37%)
Cell Cycle Regulation Yes
nucleus - S/G2 (3.7e-05)
Subcompartmental Group N/A

Sgo1

Sgo1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sgo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available