Standard name | |
---|---|
Human Ortholog | |
Description | Repressor of G1 transcription; binds to SCB binding factor (SBF) at SCB target promoters in early G1; dilution of Whi5p concentration during cell growth determines cell size; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5, and contributes to both the determination of critical cell size at START and cell fate; periodically expressed in G1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | – | – | – | 0 | 2 | 1 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 1 | 1 | 4 | 4 | 1 | 7 | 2 | 6 | 2 | 0 | 0 | 0 | – | – | – | 14 | 45 | 14 | 5 | 3 | 4 |
Bud Neck | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 2 | 2 | 0 | 0 | 0 | – | – | – | 1 | 3 | 1 | 0 | 0 | 4 |
Bud Site | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 6 | 6 | 1 | 5 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 1 | 3 | 7 | 10 | 3 | 5 | 2 | 3 | 3 | 1 | 1 | 5 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 57 | 178 | 96 | 132 | 173 | 157 | 129 | 122 | 99 | 77 | 43 | 63 | 99 | 187 | 234 | – | – | – | 37 | 119 | 69 | 13 | 5 | 8 |
Endoplasmic Reticulum | 0 | 0 | 1 | 1 | 2 | 2 | 2 | 3 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 1 | 3 | 1 |
Endosome | 1 | 0 | 4 | 5 | 5 | 8 | 8 | 6 | 1 | 0 | 1 | 2 | 2 | 0 | 4 | – | – | – | 2 | 4 | 2 | 4 | 10 | 10 |
Golgi | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | – | – | – | 0 | 1 | 1 | 0 | 1 | 2 |
Mitochondria | 1 | 11 | 0 | 9 | 8 | 39 | 42 | 34 | 115 | 61 | 84 | 87 | 0 | 2 | 0 | – | – | – | 1 | 3 | 4 | 1 | 6 | 7 |
Nucleus | 54 | 164 | 103 | 92 | 59 | 156 | 147 | 135 | 133 | 147 | 129 | 160 | 92 | 106 | 111 | – | – | – | 50 | 151 | 94 | 104 | 255 | 221 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 4 | 3 | 6 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 1 | 3 | 7 |
Nucleolus | 4 | 4 | 1 | 5 | 2 | 10 | 20 | 14 | 31 | 33 | 45 | 42 | 1 | 4 | 4 | – | – | – | 5 | 8 | 7 | 29 | 71 | 77 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 2 | 2 | 0 | 4 | 1 | 6 | 2 | 2 | 1 | 2 | 1 | 1 | 0 | – | – | – | 2 | 6 | 7 | 2 | 12 | 12 |
Vac/Vac Membrane | 6 | 12 | 6 | 7 | 13 | 22 | 22 | 27 | 11 | 12 | 11 | 14 | 0 | 3 | 8 | – | – | – | 1 | 18 | 5 | 12 | 37 | 49 |
Unique Cell Count | 119 | 357 | 211 | 240 | 250 | 370 | 348 | 320 | 326 | 304 | 262 | 309 | 192 | 298 | 356 | 120 | 369 | 213 | 184 | 420 | 419 | |||
Labelled Cell Count | 123 | 374 | 216 | 257 | 267 | 410 | 386 | 355 | 419 | 347 | 334 | 385 | 196 | 304 | 367 | 120 | 369 | 213 | 184 | 420 | 419 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.6 | 7.3 | 8.0 | 7.3 | 8.0 | 6.7 | 6.9 | 7.0 | 6.0 | 6.7 | 6.1 | 6.6 | 7.5 | 7.7 | 7.8 | – | – | – | 8.8 | 8.5 | 8.7 |
Std Deviation (1e-4) | 1.1 | 1.1 | 1.7 | 1.1 | 1.6 | 1.1 | 1.4 | 1.3 | 1.2 | 1.4 | 1.2 | 1.8 | 1.1 | 1.3 | 1.8 | – | – | – | 2.3 | 1.7 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.0 | -0.26 | -0.21 | -0.2 | -0.42 | -0.26 | -0.4 | -0.29 | -0.1 | -0.06 | -0.03 | – | – | – | 0.14 | 0.08 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | 2.0 | 5.1 | -0.7 | -2.0 | -1.7 | -3.6 | -4.8 | -6.9 | -6.1 | 1.2 | 3.9 | 4.7 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0 | 0 | 4.9 | 5.2 | 4.9 | 9.7 | 6.9 | 9.1 | 8.4 | 0 | 0 | 0 | – | – | – |
Nucleus | -2.2 | -5.6 | -1.6 | -1.5 | -1.5 | -1.8 | -0.1 | 0.1 | 0.7 | -0.2 | -3.0 | -4.2 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 3.2 | 2.7 | 4.3 | 4.7 | 6.1 | 5.3 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 1.3 | 1.7 | 1.8 | 2.6 | 0.3 | 0.7 | 0.8 | 1.0 | 0 | 0 | -0.4 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.3521 | 3.6136 | 3.4216 | 3.0261 | 2.9063 | 3.5435 | 5.0727 | 5.1567 | 5.2048 | 5.6874 | 4.2519 | 5.1871 | 2.7554 | 2.5464 | 2.7941 | 2.8738 | 2.599 | 2.6672 |
Actin | 0.0122 | 0.0036 | 0.018 | 0.0027 | 0.001 | 0.0093 | 0.0409 | 0.0017 | 0.0183 | 0.0045 | 0.0583 | 0.002 | 0.0025 | 0.0035 | 0.0044 | 0.0033 | 0.0013 | 0.002 |
Bud | 0.0005 | 0.0511 | 0.0391 | 0.0422 | 0.0218 | 0.0192 | 0.0016 | 0.032 | 0.0341 | 0.0157 | 0.0353 | 0.0307 | 0.0006 | 0.0475 | 0.0476 | 0.0223 | 0.0211 | 0.0372 |
Bud Neck | 0.0011 | 0.004 | 0.0043 | 0.0095 | 0.0106 | 0.0252 | 0.0135 | 0.0027 | 0.0057 | 0.0047 | 0.0111 | 0.0225 | 0.0011 | 0.0032 | 0.0053 | 0.0041 | 0.0028 | 0.0184 |
Bud Periphery | 0.0007 | 0.0287 | 0.0274 | 0.0412 | 0.0203 | 0.0266 | 0.0027 | 0.0157 | 0.028 | 0.0108 | 0.0531 | 0.0368 | 0.0003 | 0.0256 | 0.0308 | 0.0208 | 0.0091 | 0.0349 |
Bud Site | 0.0054 | 0.1207 | 0.039 | 0.0129 | 0.0056 | 0.0075 | 0.0158 | 0.0701 | 0.037 | 0.0135 | 0.0231 | 0.0099 | 0.0011 | 0.0974 | 0.0353 | 0.0063 | 0.0033 | 0.011 |
Cell Periphery | 0.0002 | 0.0031 | 0.0024 | 0.0021 | 0.0011 | 0.0018 | 0.0015 | 0.0021 | 0.0024 | 0.0009 | 0.0018 | 0.0012 | 0.0001 | 0.0024 | 0.0021 | 0.0008 | 0.0004 | 0.0011 |
Cytoplasm | 0.0145 | 0.3671 | 0.6549 | 0.577 | 0.6273 | 0.109 | 0.0444 | 0.3796 | 0.6179 | 0.6393 | 0.4623 | 0.125 | 0.0537 | 0.4704 | 0.6459 | 0.6935 | 0.8276 | 0.2308 |
Cytoplasmic Foci | 0.0044 | 0.0087 | 0.016 | 0.013 | 0.0118 | 0.0063 | 0.0256 | 0.003 | 0.0144 | 0.0142 | 0.0579 | 0.0031 | 0.0011 | 0.0043 | 0.0051 | 0.0157 | 0.0253 | 0.0021 |
Eisosomes | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0013 | 0 | 0.0002 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0018 | 0.0078 | 0.0078 | 0.0075 | 0.0041 | 0.0018 | 0.0163 | 0.0032 | 0.0054 | 0.005 | 0.0048 | 0.0016 | 0.0007 | 0.0092 | 0.0049 | 0.0049 | 0.0039 | 0.0016 |
Endosome | 0.0099 | 0.0085 | 0.0131 | 0.0292 | 0.0218 | 0.0087 | 0.0333 | 0.0027 | 0.0162 | 0.0456 | 0.0346 | 0.0052 | 0.0015 | 0.0049 | 0.0062 | 0.0285 | 0.0193 | 0.0027 |
Golgi | 0.0032 | 0.0007 | 0.0041 | 0.002 | 0.0014 | 0.0053 | 0.0104 | 0.0003 | 0.006 | 0.0155 | 0.0221 | 0.002 | 0.0004 | 0.0004 | 0.0006 | 0.001 | 0.0007 | 0.0002 |
Lipid Particles | 0.005 | 0.0006 | 0.0057 | 0.0011 | 0.0286 | 0.0037 | 0.0277 | 0.0001 | 0.0027 | 0.0152 | 0.0095 | 0.0067 | 0.0004 | 0.0003 | 0.0006 | 0.0002 | 0.0006 | 0.0001 |
Mitochondria | 0.0089 | 0.0046 | 0.0054 | 0.0023 | 0.0016 | 0.004 | 0.0244 | 0.001 | 0.0052 | 0.005 | 0.0139 | 0.0018 | 0.0006 | 0.0011 | 0.0017 | 0.0022 | 0.0005 | 0.0017 |
None | 0.022 | 0.0393 | 0.0263 | 0.0385 | 0.0289 | 0.0016 | 0.0127 | 0.0138 | 0.036 | 0.063 | 0.0118 | 0.0025 | 0.003 | 0.017 | 0.0378 | 0.0113 | 0.0153 | 0.003 |
Nuclear Periphery | 0.0081 | 0.0107 | 0.0062 | 0.0053 | 0.0056 | 0.0044 | 0.0118 | 0.005 | 0.0043 | 0.003 | 0.0058 | 0.0057 | 0.0017 | 0.0084 | 0.0069 | 0.011 | 0.0037 | 0.0061 |
Nucleolus | 0.0076 | 0.0105 | 0.0025 | 0.0058 | 0.0038 | 0.0327 | 0.0077 | 0.0043 | 0.0046 | 0.001 | 0.0057 | 0.0188 | 0.006 | 0.0034 | 0.0024 | 0.0072 | 0.001 | 0.0139 |
Nucleus | 0.8798 | 0.2958 | 0.0854 | 0.1376 | 0.1716 | 0.7121 | 0.6733 | 0.4444 | 0.1379 | 0.0969 | 0.0995 | 0.708 | 0.9201 | 0.2814 | 0.1371 | 0.1389 | 0.0412 | 0.6196 |
Peroxisomes | 0.0046 | 0.0005 | 0.0091 | 0.0003 | 0.001 | 0.0058 | 0.0159 | 0.0001 | 0.0035 | 0.0243 | 0.0326 | 0.0004 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0002 |
Punctate Nuclear | 0.0066 | 0.0058 | 0.0068 | 0.0022 | 0.0013 | 0.0026 | 0.0069 | 0.002 | 0.0032 | 0.0015 | 0.0234 | 0.0082 | 0.0042 | 0.0034 | 0.003 | 0.0054 | 0.0067 | 0.0061 |
Vacuole | 0.0022 | 0.0255 | 0.0239 | 0.0629 | 0.0272 | 0.0113 | 0.0099 | 0.015 | 0.0153 | 0.0181 | 0.0295 | 0.007 | 0.0006 | 0.0143 | 0.0194 | 0.0196 | 0.0143 | 0.0065 |
Vacuole Periphery | 0.0011 | 0.0026 | 0.0025 | 0.0047 | 0.0036 | 0.0013 | 0.0025 | 0.0011 | 0.0018 | 0.0023 | 0.0033 | 0.0009 | 0.0002 | 0.0016 | 0.0025 | 0.0028 | 0.0015 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.5322 | 24.4372 | 9.0667 | 9.0424 | 26.5823 | 18.6283 | 14.3197 | 10.9914 | 15.8273 | 16.5153 |
Translational Efficiency | 1.4584 | 0.9654 | 0.9657 | 1.4461 | 0.9746 | 1.224 | 1.48 | 1.6067 | 0.987 | 1.0799 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2376 | 1377 | 2621 | 1234 | 1645 | 2085 | 1447 | 2097 | 4021 | 3462 | 4068 | 3331 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 836.69 | 884.58 | 999.21 | 1000.64 | 776.36 | 899.21 | 1054.89 | 979.72 | 812.01 | 893.39 | 1019.02 | 987.47 |
Standard Deviation | 111.36 | 122.53 | 126.63 | 131.76 | 103.57 | 129.17 | 136.20 | 130.12 | 112.23 | 126.78 | 132.82 | 131.12 |
Intensity Change Log 2 | — | 0.080299 | 0.256095 | 0.258158 | — | 0.211932 | 0.442295 | 0.335644 | — | 0.145155 | 0.348715 | 0.295972 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000149 | 0.000604 | 0.000345 | 0.000714 | 0.000248 | 0.000251 | 0.000975 | 0.000730 | 0.000189 | 0.000391 | 0.000569 | 0.000724 |
Bud Neck | 0.030251 | 0.035154 | 0.033296 | 0.014137 | 0.024063 | 0.027738 | 0.024138 | 0.013951 | 0.027719 | 0.030688 | 0.030038 | 0.014020 |
Bud Site | 0.008276 | 0.018528 | 0.013286 | 0.027758 | 0.010227 | 0.009409 | 0.009815 | 0.020642 | 0.009074 | 0.013036 | 0.012051 | 0.023278 |
Cell Periphery | 0.000547 | 0.000454 | 0.000247 | 0.000250 | 0.000449 | 0.000380 | 0.000336 | 0.000204 | 0.000507 | 0.000409 | 0.000279 | 0.000221 |
Cytoplasm | 0.305212* | 0.333833* | 0.411329* | 0.508211* | 0.305315* | 0.305326* | 0.265895* | 0.613872* | 0.305254* | 0.316664* | 0.359598* | 0.574729* |
Cytoplasmic Foci | 0.023510 | 0.033766 | 0.009023 | 0.031066 | 0.031426 | 0.019582 | 0.011587 | 0.018396 | 0.026749 | 0.025224 | 0.009935 | 0.023090 |
Eisosomes | 0.000034 | 0.000032 | 0.000019 | 0.000024 | 0.000029 | 0.000027 | 0.000034 | 0.000017 | 0.000032 | 0.000029 | 0.000024 | 0.000020 |
Endoplasmic Reticulum | 0.001851 | 0.003435 | 0.001326 | 0.002879 | 0.002068 | 0.001771 | 0.002970 | 0.001452 | 0.001940 | 0.002433 | 0.001911 | 0.001981 |
Endosome | 0.005448 | 0.006720 | 0.001271 | 0.005091 | 0.006065 | 0.004893 | 0.002185 | 0.002680 | 0.005701 | 0.005620 | 0.001596 | 0.003573 |
Golgi | 0.000487 | 0.001633 | 0.000195 | 0.005143 | 0.000897 | 0.000965 | 0.000799 | 0.002815 | 0.000654 | 0.001231 | 0.000410 | 0.003678 |
Lipid Particles | 0.001204 | 0.000942 | 0.000218 | 0.000535 | 0.000960 | 0.000726 | 0.000453 | 0.000315 | 0.001104 | 0.000812 | 0.000302 | 0.000397 |
Mitochondria | 0.001937 | 0.002361 | 0.001690 | 0.001403 | 0.001304 | 0.002662 | 0.001365 | 0.001035 | 0.001678 | 0.002542 | 0.001575 | 0.001171 |
Mitotic Spindle | 0.002082 | 0.007853 | 0.027061 | 0.047594 | 0.003637 | 0.008954 | 0.017607 | 0.028678 | 0.002718 | 0.008516 | 0.023699 | 0.035686 |
None | 0.016123 | 0.011530 | 0.010181 | 0.005844 | 0.014832 | 0.013208 | 0.006838 | 0.007828 | 0.015595 | 0.012541 | 0.008992 | 0.007093 |
Nuclear Periphery | 0.000848 | 0.000668 | 0.001028 | 0.000987 | 0.000506 | 0.000857 | 0.000922 | 0.000731 | 0.000708 | 0.000782 | 0.000990 | 0.000826 |
Nuclear Periphery Foci | 0.000511 | 0.001326 | 0.000543 | 0.001630 | 0.000731 | 0.000458 | 0.001933 | 0.000549 | 0.000601 | 0.000803 | 0.001038 | 0.000950 |
Nucleolus | 0.015118 | 0.021840 | 0.005992 | 0.001854 | 0.016801 | 0.015864 | 0.008336 | 0.002773 | 0.015807 | 0.018241 | 0.006826 | 0.002432 |
Nucleus | 0.554691* | 0.488161* | 0.456701* | 0.257992* | 0.553970* | 0.561719* | 0.606908* | 0.228882* | 0.554396* | 0.532462* | 0.510130* | 0.239666* |
Peroxisomes | 0.000599 | 0.001486 | 0.000187 | 0.000782 | 0.000799 | 0.000973 | 0.000354 | 0.000501 | 0.000681 | 0.001177 | 0.000246 | 0.000605 |
Vacuole | 0.029922 | 0.027125 | 0.025626 | 0.084957 | 0.024205 | 0.023154 | 0.036176 | 0.052039 | 0.027583 | 0.024734 | 0.029379 | 0.064234 |
Vacuole Periphery | 0.001201 | 0.002549 | 0.000433 | 0.001148 | 0.001467 | 0.001082 | 0.000374 | 0.001911 | 0.001310 | 0.001665 | 0.000412 | 0.001628 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.66 | -13.43 | -10.42 | -3.30 | -5.31 | 0.30 | -9.51 | -2.83 | -2.90 | 2.65 | -6.49 | -14.41 | -5.99 | -3.81 | -0.37 |
Bud Neck | -2.42 | -8.37 | 6.76 | 7.64 | 14.85 | -2.25 | -4.60 | 1.44 | 3.97 | 6.33 | -2.53 | -9.81 | 5.74 | 7.97 | 15.46 |
Bud Site | -8.37 | -5.98 | -6.29 | 0.79 | -2.13 | 0.84 | -1.66 | -4.54 | -5.54 | -3.55 | -5.73 | -5.76 | -7.91 | -3.54 | -3.49 |
Cell Periphery | 1.70 | 9.09 | 8.94 | 5.61 | 1.04 | 1.60 | 3.14 | 6.91 | 6.21 | 6.40 | 2.89 | 9.26 | 11.69 | 8.40 | 5.22 |
Cytoplasm | -2.10 | -2.26 | -7.48 | -4.67 | -5.80 | 0.07 | 6.51 | -17.54 | -18.55 | -25.28 | -1.16 | 1.75 | -19.68 | -17.59 | -21.83 |
Cytoplasmic Foci | -3.08 | 9.68 | 1.50 | 4.31 | -8.44 | 4.28 | 8.93 | 6.45 | 2.43 | -4.76 | 1.08 | 13.25 | 6.19 | 4.91 | -9.94 |
Eisosomes | 0.54 | 5.20 | -1.39 | -1.82 | -6.58 | 0.89 | -4.75 | 3.10 | 3.14 | 9.53 | 1.70 | 1.05 | 2.67 | 1.07 | 2.11 |
Endoplasmic Reticulum | -5.30 | -1.18 | -7.78 | -2.14 | -7.60 | 1.59 | -7.95 | -0.35 | -1.87 | 7.25 | -3.33 | -5.76 | -5.71 | -2.52 | -1.69 |
Endosome | -1.51 | 10.69 | 5.38 | 5.56 | -3.23 | 1.77 | 7.60 | 7.45 | 6.24 | 0.22 | 0.34 | 13.15 | 9.67 | 8.42 | -2.61 |
Golgi | -3.12 | 3.71 | -1.38 | 0.11 | -1.71 | -0.11 | 1.10 | -0.82 | -0.70 | -1.46 | -2.18 | 2.33 | -1.75 | -0.43 | -2.49 |
Lipid Particles | 1.24 | 5.61 | 2.41 | 0.96 | -7.35 | 1.33 | 3.50 | 4.51 | 2.39 | 2.72 | 2.06 | 6.71 | 5.02 | 2.59 | -5.03 |
Mitochondria | -0.74 | 0.20 | 1.12 | 2.44 | 2.45 | -2.40 | -1.80 | 0.02 | 2.54 | 3.98 | -1.96 | -0.37 | 1.19 | 3.33 | 4.01 |
Mitotic Spindle | -4.11 | -13.52 | -10.92 | -8.87 | -3.85 | -3.41 | -7.80 | -10.90 | -8.32 | -3.29 | -5.50 | -15.47 | -15.63 | -12.07 | -4.46 |
None | 3.48 | 6.40 | 10.07 | 6.22 | 9.04 | 1.35 | 8.23 | 6.63 | 5.56 | -3.13 | 3.34 | 9.71 | 11.35 | 7.99 | 4.35 |
Nuclear Periphery | 1.11 | -3.21 | -5.50 | -8.31 | -4.22 | -3.18 | -11.31 | -6.16 | -1.59 | 1.29 | -1.02 | -7.01 | -6.29 | -5.06 | -1.00 |
Nuclear Periphery Foci | -2.73 | -0.21 | -3.79 | 0.28 | -3.87 | 2.00 | -3.86 | 1.92 | -0.58 | 5.60 | -1.64 | -2.91 | -2.18 | 0.20 | 0.49 |
Nucleolus | -3.97 | 4.12 | 11.20 | 12.04 | 7.93 | 0.42 | 3.16 | 9.71 | 9.74 | 5.53 | -2.46 | 5.31 | 14.12 | 15.19 | 8.86 |
Nucleus | 5.02 | 4.17 | 17.12 | 10.66 | 14.53 | -0.57 | -4.31 | 20.79 | 23.00 | 26.26 | 2.40 | 0.87 | 28.18 | 24.53 | 28.76 |
Peroxisomes | -4.47 | 3.89 | 1.67 | 5.59 | -3.25 | -0.55 | 2.36 | 2.17 | 2.26 | -0.16 | -2.90 | 4.38 | 2.64 | 4.71 | -2.35 |
Vacuole | 0.96 | -1.51 | -18.08 | -18.48 | -17.45 | 0.31 | -6.39 | -14.70 | -15.42 | -6.33 | 1.41 | -5.30 | -22.48 | -23.64 | -18.12 |
Vacuole Periphery | -2.83 | 9.43 | 5.26 | 4.31 | -1.08 | 2.27 | 7.58 | 1.43 | 0.29 | -2.03 | -1.64 | 11.85 | 2.39 | 3.08 | -2.16 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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