Standard name | |
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Human Ortholog | |
Description | Vacuolar cation channel; mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 1 | 4 | 1 | 1 | 5 | 5 | 4 | 5 | 1 | 1 | 5 | 0 | 0 | 0 | 0 | 2 | 5 | 1 | 2 | 4 | 1 | 1 | 2 |
Bud | 3 | 7 | 5 | 0 | 1 | 2 | 4 | 7 | 8 | 8 | 7 | 5 | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 4 | 2 | 2 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 15 | 22 | 31 | 15 | 14 | 16 | 16 | 7 | 5 | 5 | 5 | 8 | 8 | 77 | 57 | 3 | 12 | 8 | 9 | 3 | 21 | 3 | 3 | 1 |
Cytoplasm | 72 | 151 | 87 | 54 | 113 | 168 | 187 | 186 | 100 | 107 | 162 | 159 | 22 | 193 | 162 | 53 | 71 | 87 | 44 | 136 | 48 | 74 | 145 | 104 |
Endoplasmic Reticulum | 8 | 6 | 19 | 11 | 17 | 15 | 17 | 1 | 6 | 1 | 2 | 14 | 1 | 20 | 16 | 0 | 3 | 1 | 40 | 30 | 50 | 27 | 50 | 42 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 1 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 3 | 0 | 0 | 0 | 5 | 7 | 9 | 19 | 29 | 55 | 30 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 2 | 1 | 0 | 0 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 3 | 4 | 3 | 1 | 2 | 3 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 1 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | 1 | 1 | 3 | 5 | 2 | 10 | 2 | 0 | 6 | 3 | 0 | 0 | 0 | 1 | 3 | 3 | 0 | 4 | 5 | 2 | 5 | 6 |
Unique Cell Count | 88 | 169 | 118 | 69 | 131 | 198 | 220 | 209 | 123 | 130 | 212 | 204 | 24 | 224 | 181 | 56 | 82 | 102 | 103 | 191 | 140 | 118 | 216 | 174 |
Labelled Cell Count | 103 | 192 | 149 | 82 | 150 | 220 | 242 | 230 | 148 | 156 | 245 | 229 | 32 | 292 | 235 | 59 | 94 | 110 | 103 | 191 | 140 | 118 | 216 | 174 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.0 | 7.2 | 10.1 | 10.9 | 10.5 | 8.0 | 7.2 | 6.8 | 5.1 | 5.3 | 5.1 | 5.3 | 12.1 | 11.4 | 10.8 | 7.3 | 8.6 | 9.3 | 11.5 | 11.8 | 10.9 |
Std Deviation (1e-4) | 2.1 | 1.5 | 1.7 | 2.2 | 1.6 | 1.6 | 1.5 | 1.3 | 1.0 | 1.2 | 1.0 | 1.0 | 2.0 | 1.6 | 1.6 | 1.9 | 1.4 | 1.6 | 1.7 | 2.1 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.1 | 0.05 | -0.34 | -0.5 | -0.57 | -1.0 | -0.93 | -0.99 | -0.94 | 0.26 | 0.18 | 0.1 | -0.47 | -0.24 | -0.13 | 0.19 | 0.22 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0.8 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.7 | -3.2 | -4.4 | -4.8 | -6.2 | -4.8 | -5.0 | -6.7 | -5.9 | 0.7 | 1.5 | 1.0 | -3.3 | -2.0 | -3.6 |
Cytoplasm | 0.7 | 2.5 | 2.4 | 2.5 | 3.6 | 1.4 | 1.6 | 0.5 | 0.9 | 1.9 | 2.8 | 3.6 | 3.3 | 2.2 | 2.1 |
Endoplasmic Reticulum | 0 | -0.7 | -2.4 | -2.4 | -5.7 | -2.9 | -4.4 | -5.4 | -2.6 | 0 | -2.0 | -1.9 | -3.2 | -2.8 | -3.9 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4.4 | 5.5 | 6.1 | 4.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.0945 | 10.2838 | 9.8465 | 9.1997 | 9.2048 | 9.5633 | 11.2021 | 13.5026 | 12.1925 | 11.4188 | 11.2297 | 12.2067 | 10.3393 | 11.452 | 10.7198 | 10.2476 | 8.9475 | 10.4216 |
Actin | 0.0033 | 0.0016 | 0.0027 | 0.0008 | 0.016 | 0.0027 | 0.0455 | 0.0027 | 0.0155 | 0.0133 | 0.0029 | 0.0092 | 0.0179 | 0.0005 | 0.0061 | 0.0015 | 0.0215 | 0.0019 |
Bud | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.001 | 0.0007 | 0.0001 | 0.0004 | 0.0007 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0001 |
Bud Neck | 0.0035 | 0.001 | 0.0033 | 0.0038 | 0.0021 | 0.0105 | 0.0025 | 0.0032 | 0.004 | 0.0028 | 0.0027 | 0.0075 | 0.0017 | 0.0008 | 0.0035 | 0.0103 | 0.0056 | 0.008 |
Bud Periphery | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0009 | 0.0003 | 0.0026 | 0.0021 | 0.0004 | 0.0007 | 0.0019 | 0.0001 | 0.001 | 0.0007 | 0.0008 | 0.0003 |
Bud Site | 0.0058 | 0.0072 | 0.0012 | 0.0003 | 0.0006 | 0.0002 | 0.0094 | 0.0034 | 0.0105 | 0.0061 | 0.0003 | 0.0005 | 0.0044 | 0.0023 | 0.0059 | 0.0013 | 0.0023 | 0.0002 |
Cell Periphery | 0.0122 | 0.0097 | 0.013 | 0.0274 | 0.0042 | 0.0084 | 0.0206 | 0.024 | 0.022 | 0.0199 | 0.0136 | 0.0147 | 0.024 | 0.0157 | 0.0149 | 0.0214 | 0.0183 | 0.0053 |
Cytoplasm | 0.7471 | 0.8141 | 0.787 | 0.8069 | 0.7811 | 0.8332 | 0.6159 | 0.8661 | 0.7089 | 0.6507 | 0.7907 | 0.7401 | 0.7123 | 0.8843 | 0.8345 | 0.8079 | 0.7757 | 0.8701 |
Cytoplasmic Foci | 0.0033 | 0.0018 | 0.0022 | 0.0013 | 0.0058 | 0.0021 | 0.0156 | 0.0024 | 0.0121 | 0.0432 | 0.0139 | 0.0071 | 0.0081 | 0.0008 | 0.0013 | 0.0019 | 0.0233 | 0.0022 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.181 | 0.1514 | 0.1696 | 0.149 | 0.1483 | 0.1266 | 0.1948 | 0.0756 | 0.1673 | 0.1169 | 0.0841 | 0.168 | 0.1577 | 0.0868 | 0.1146 | 0.1135 | 0.088 | 0.0954 |
Endosome | 0.0041 | 0.0006 | 0.0037 | 0.0004 | 0.0065 | 0.0031 | 0.0244 | 0.0016 | 0.0135 | 0.0569 | 0.0168 | 0.0137 | 0.0158 | 0.0002 | 0.0016 | 0.0219 | 0.0169 | 0.0047 |
Golgi | 0.0024 | 0.0004 | 0.0024 | 0.0002 | 0.0017 | 0.0007 | 0.0124 | 0.0013 | 0.009 | 0.0294 | 0.0064 | 0.012 | 0.0078 | 0.0001 | 0.0008 | 0.0031 | 0.0111 | 0.0027 |
Lipid Particles | 0.0092 | 0.0053 | 0.006 | 0.0038 | 0.0023 | 0.0023 | 0.0159 | 0.0111 | 0.0083 | 0.0152 | 0.0275 | 0.0057 | 0.0124 | 0.0036 | 0.0022 | 0.0058 | 0.0091 | 0.002 |
Mitochondria | 0.004 | 0.0001 | 0.0013 | 0 | 0.0004 | 0.0004 | 0.0048 | 0.0001 | 0.0078 | 0.0127 | 0.0009 | 0.0083 | 0.0141 | 0 | 0.0003 | 0.001 | 0.0067 | 0.0012 |
None | 0.0027 | 0.002 | 0.0026 | 0.0003 | 0.0019 | 0.0007 | 0.0082 | 0.0038 | 0.0018 | 0.0012 | 0.0191 | 0.0035 | 0.003 | 0.0021 | 0.0012 | 0.0007 | 0.0014 | 0.0008 |
Nuclear Periphery | 0.0052 | 0.0015 | 0.0016 | 0.002 | 0.0074 | 0.0049 | 0.0118 | 0.0008 | 0.002 | 0.0028 | 0.0009 | 0.0019 | 0.0045 | 0.0008 | 0.0013 | 0.0012 | 0.0012 | 0.0016 |
Nucleolus | 0.0008 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0.0005 | 0 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 |
Nucleus | 0.0044 | 0.0007 | 0.0006 | 0.0007 | 0.003 | 0.0011 | 0.0016 | 0.0004 | 0.0017 | 0.0009 | 0.0005 | 0.0011 | 0.0041 | 0.0004 | 0.0006 | 0.0006 | 0.0006 | 0.0011 |
Peroxisomes | 0.002 | 0 | 0.0003 | 0 | 0.0012 | 0 | 0.0032 | 0.0001 | 0.0051 | 0.0078 | 0.0132 | 0.0031 | 0.0022 | 0 | 0 | 0 | 0.0135 | 0.0009 |
Punctate Nuclear | 0.0048 | 0.0004 | 0.0002 | 0.0001 | 0.0158 | 0.0004 | 0.0045 | 0.0001 | 0.0007 | 0.0015 | 0.0027 | 0.0005 | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0.0012 | 0.0005 |
Vacuole | 0.0032 | 0.0013 | 0.002 | 0.0026 | 0.0009 | 0.0017 | 0.0051 | 0.0027 | 0.0052 | 0.014 | 0.0026 | 0.0019 | 0.0053 | 0.0012 | 0.0071 | 0.0061 | 0.0015 | 0.001 |
Vacuole Periphery | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0014 | 0 | 0.0006 | 0.0012 | 0.0002 | 0.0003 | 0.0009 | 0 | 0.0025 | 0.0008 | 0.0005 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.5946 | 18.4824 | 20.933 | 21.9721 | 14.814 | 27.1892 | 23.8512 | 25.7235 | 24.3427 | 21.955 |
Translational Efficiency | 0.6336 | 0.7161 | 0.7047 | 0.6863 | 0.7137 | 0.4986 | 0.7632 | 0.5741 | 0.7198 | 0.5797 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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