Standard name
Human Ortholog
Description Glucose-repressible ADP-ribosylation factor; GTPase of Ras superfamily involved in regulating cell polarity and invasive growth; localizes to dynamic spots at plasma membrane and modulates PtdIns(4,5)P2 levels to facilitate endocytosis; required for localization of endocytic protein Lsb5p to correct cortical site in cells; also has mRNA binding activity; homolog of mammalian Arf6
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.3 0.25 0.34 0.14 0.28 0.21 0.15 0.1 0.08 0.12 0.06 0.08 – 0.3 0.29 0.17 0.2 0.14 0.08 0.14 0.1 0.06 0.1 0.08
Bud 0.12 0.14 0.22 0.1 0.19 0.16 0.13 0.12 0.12 0.12 0.1 0.11 – 0.12 0.07 0.11 0.13 0.08 0.06 0.05 0.05 0 0.07 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.17 0.26 0.17 0.16 0.11 0.14
Bud Site 0.07 0.06 0.06 0 0.06 0 0 0 0 0 0 0 – 0.06 0.05 0.09 0.07 0 – – – – – –
Cell Periphery 0.65 0.52 0.52 0.53 0.28 0.46 0.37 0.25 0.43 0.32 0.38 0.37 – 0.51 0.5 0.12 0.19 0.22 0.52 0.31 0.5 0.51 0.45 0.42
Cytoplasm 0.35 0.45 0.33 0.58 0.48 0.53 0.59 0.57 0.65 0.65 0.7 0.63 – 0.38 0.37 0.36 0.26 0.34 0.06 0.14 0.06 0.09 0.09 0.15
Endoplasmic Reticulum 0 0 0 0.06 0.08 0.06 0.06 0 0 0 0 0 – 0 0 0 0 0 0.08 0.07 0.08 0 0.14 0.09
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0.05 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.07 0.09 0.09 0.1 0.1 0.14 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.09 0.12 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0
SpindlePole 0 0 0 0.06 0 0 0 0 0 0 0 0.06 – 0 0.09 0.27 0.31 0.3 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0.05 0 0 – 0 0.05 0.05 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 77 49 50 27 84 71 80 52 32 65 27 43 – 31 48 45 46 31 25 35 15 3 8 8
Bud 31 27 32 19 55 52 69 65 45 60 44 56 – 12 12 31 29 17 17 13 8 0 5 6
Bud Neck 1 0 0 1 1 0 0 0 0 0 0 0 – 0 0 1 7 0 53 65 26 9 9 16
Bud Site 18 11 9 6 17 9 17 5 2 8 6 8 – 6 9 23 16 10 – – – – – –
Cell Periphery 168 103 77 102 84 154 197 131 166 165 160 198 – 52 82 33 42 49 162 78 81 29 38 48
Cytoplasm 91 90 49 112 141 178 315 299 248 341 294 334 – 39 60 97 58 76 18 35 8 5 7 17
Endoplasmic Reticulum 9 7 5 12 23 21 34 10 14 11 8 10 – 2 5 12 5 8 26 17 12 2 11 9
Endosome 0 1 1 5 6 0 3 11 2 7 11 4 – 0 9 7 11 12 1 1 1 0 0 0
Golgi 2 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 3 0 1 1 0 1 0 0
Mitochondria 0 0 2 0 1 13 38 46 35 53 42 73 – 0 0 10 6 6 1 0 1 1 1 0
Nucleus 0 2 0 0 1 1 3 6 1 2 1 2 – 0 1 9 5 3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 2 17 1 1 3 1 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 3 0 2 1 3 23 5 6 7 15 – 1 3 24 26 12 0 0 0 0 0 0
Peroxisomes 0 1 0 2 0 1 0 2 2 1 3 6 – 1 0 5 6 12 0 0 0 0 0 0
SpindlePole 2 3 6 11 11 2 5 10 6 19 14 30 – 3 14 74 69 68 0 0 2 0 0 2
Vac/Vac Membrane 0 2 3 4 8 3 23 29 16 28 16 13 – 0 8 14 14 10 2 3 0 2 0 0
Unique Cell Count 259 199 148 194 295 334 538 520 382 521 423 529 102 164 270 225 223 312 255 161 57 85 114
Labelled Cell Count 400 296 237 301 434 506 789 706 575 767 636 793 147 251 385 343 314 312 255 161 57 85 114


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.8 13.0 9.4 9.3 9.8 9.2 8.8 8.9 9.2 8.8 8.8 9.4 – 11.5 11.5 12.5 13.5 13.3 12.9 13.0 13.3
Std Deviation (1e-4) 2.4 2.3 1.8 1.9 2.0 1.8 2.0 2.1 2.2 2.1 2.1 2.4 – 1.7 2.0 3.7 3.9 3.8 2.4 2.4 2.4
Intensity Change (Log2) – – – -0.02 0.06 -0.02 -0.09 -0.07 -0.02 -0.09 -0.1 0.01 – 0.3 0.3 0.42 0.53 0.5 0.46 0.48 0.51

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP700051015WT3HU120HU160051015WT3rpd3Δ_1rpd3Δ_2rpd3Δ_3051015WT1AF100AF140AF180051015
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -4.4 -1.1 -2.9 -5.2 -7.1 -7.3 -6.1 -8.4 -8.0 – -0.6 -0.9 -4.0 -2.9 -4.5
Bud -3.0 -0.7 -1.6 -2.7 -2.8 -2.9 -3.2 -3.5 -3.5 – -2.0 -3.6 -2.8 -2.2 -3.9
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site -1.3 -0.1 -1.8 -1.6 0 0 0 0 0 – -0.1 -0.2 0.9 0.4 -0.7
Cell Periphery 0.1 -4.9 -1.2 -3.4 -6.2 -1.8 -4.5 -3.0 -3.2 – -0.2 -0.4 -8.8 -6.8 -6.0
Cytoplasm 4.5 2.9 4.1 5.5 5.2 6.6 7.0 7.8 6.5 – 0.8 0.6 0.6 -1.5 0.2
Endoplasmic Reticulum 1.2 1.8 1.3 1.4 0 0.2 0 0 0 – 0 0 0.5 0 0.1
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 2.4
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 0 2.6 3.1 3.2 3.4 3.4 4.3 – 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 1.3 0 0 0 0 – 0 0 2.7 3.4 1.6
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0.7 -0.2 0 0 0 0 -0.2 -0.4 0.8 – 0 1.6 5.8 6.3 6.2
Vacuole 0 0 0 1.3 1.8 1.2 1.7 0 0 – 0 1.4 1.6 1.9 1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.473 7.1773 6.4071 5.7559 5.9601 6.7164 7.5676 7.1462 6.5656 7.3165 5.9159 6.9241 6.4026 5.6983 5.6598 5.9469 5.3652 5.3707
Actin 0.0474 0.0066 0.0274 0.1195 0.0182 0.0215 0.0403 0.0082 0.0478 0.0586 0.0654 0.0069 0.0651 0.0089 0.0119 0.0066 0.0065 0.0193
Bud 0.0029 0.0055 0.0173 0.0058 0.0063 0.0034 0.0009 0.0014 0.0107 0.0043 0.0017 0.0024 0.0007 0.0028 0.0082 0.0112 0.0011 0.0011
Bud Neck 0.0169 0.0124 0.0604 0.0668 0.0614 0.1334 0.0111 0.0062 0.0429 0.0486 0.0132 0.1107 0.0152 0.0172 0.0511 0.0797 0.0838 0.1247
Bud Periphery 0.017 0.027 0.1845 0.0884 0.1146 0.0461 0.0045 0.005 0.1039 0.0907 0.0225 0.0279 0.0033 0.0084 0.0965 0.1756 0.019 0.019
Bud Site 0.0503 0.3667 0.1673 0.0158 0.0269 0.0083 0.0468 0.3875 0.1383 0.0192 0.0086 0.0074 0.0522 0.3899 0.1599 0.0047 0.0122 0.0019
Cell Periphery 0.5028 0.2952 0.3666 0.3657 0.5146 0.5096 0.5955 0.4151 0.4956 0.5852 0.5274 0.6276 0.5088 0.3439 0.5082 0.5498 0.4635 0.5225
Cytoplasm 0.2217 0.2598 0.0998 0.1498 0.0803 0.2244 0.1956 0.1647 0.0926 0.1628 0.1994 0.1807 0.2674 0.2009 0.1327 0.1379 0.2796 0.2762
Cytoplasmic Foci 0.0153 0.0005 0.0117 0.0232 0.0298 0.0031 0.0109 0.0002 0.0166 0.0026 0.0213 0.0061 0.012 0.0005 0.0008 0.0001 0.0045 0.0017
Eisosomes 0.0026 0.0006 0.003 0.0168 0.0005 0.0014 0.0025 0.0006 0.0008 0.0006 0.0081 0.0011 0.002 0.0042 0.0032 0.0001 0.0006 0.0005
Endoplasmic Reticulum 0.0292 0.0135 0.0102 0.018 0.0196 0.0158 0.0137 0.0038 0.0032 0.0047 0.0133 0.0094 0.0251 0.0077 0.0079 0.0197 0.0291 0.0148
Endosome 0.017 0.0006 0.0038 0.0167 0.0157 0.0055 0.0203 0 0.0021 0.0006 0.0161 0.0021 0.0094 0.0003 0.001 0 0.0104 0.001
Golgi 0.0089 0.0004 0.0034 0.0197 0.0087 0.0042 0.0065 0.0001 0.0045 0.0029 0.0388 0.0019 0.0073 0.0003 0.0006 0.0001 0.0107 0.0023
Lipid Particles 0.0105 0.0019 0.0021 0.0235 0.0207 0.0023 0.0081 0.0007 0.0068 0.002 0.018 0.0048 0.0034 0.0019 0.0017 0.0015 0.0393 0.0036
Mitochondria 0.0041 0.0001 0.0124 0.023 0.0055 0.0018 0.005 0 0.0007 0.0008 0.0058 0.0008 0.0008 0.0003 0.0001 0 0.0235 0.0017
None 0.0091 0.0004 0.0014 0.0013 0.0023 0.0023 0.0118 0.0006 0.0054 0.0011 0.0023 0.0011 0.0076 0.0008 0.002 0.0001 0.0005 0.0013
Nuclear Periphery 0.0097 0.0013 0.0018 0.0016 0.0028 0.0033 0.0101 0.0006 0.0017 0.0004 0.0011 0.0005 0.0087 0.001 0.0012 0.0009 0.0013 0.0004
Nucleolus 0.0014 0.0001 0.0035 0.0028 0.0009 0.0004 0.0005 0.0002 0.0011 0.0002 0.0001 0.0013 0.0002 0.0008 0.0002 0.0001 0.0003 0.0001
Nucleus 0.0079 0.0009 0.0028 0.0018 0.0015 0.0026 0.0033 0.0007 0.0019 0.0007 0.0004 0.0007 0.001 0.0009 0.0012 0.0006 0.0005 0.0006
Peroxisomes 0.0059 0 0.0095 0.0205 0.051 0.0012 0.0053 0 0.0103 0.0023 0.0327 0.0009 0.0015 0.0001 0 0 0.005 0.0023
Punctate Nuclear 0.0053 0.0001 0.0007 0.0007 0.0068 0.0006 0.0023 0.0001 0.0077 0.0001 0.0005 0.0004 0.0011 0.0001 0.0002 0 0.0003 0.0002
Vacuole 0.0128 0.0062 0.0099 0.0151 0.0115 0.0075 0.0046 0.0043 0.0052 0.0113 0.0031 0.0054 0.0067 0.009 0.0111 0.011 0.0078 0.0047
Vacuole Periphery 0.0011 0.0002 0.0004 0.0034 0.0003 0.0011 0.0007 0 0.0001 0.0001 0.0002 0.0001 0.0006 0.0001 0.0002 0.0001 0.0009 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.9924 32.1644 18.5635 20.5625 12.9584 19.9781 17.6158 18.2915 19.096 17.9601
Translational Efficiency 2.1994 1.2518 1.7294 2.2528 2.6976 1.4791 2.0592 2.102 2.0876 1.7182

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1138 1446 1390 1211 1459 1543 1838 1607 2597 2989 3228 2818

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 906.98 1161.84 1277.90 1458.71 944.33 977.10 1264.09 1446.65 927.96 1066.47 1270.04 1451.83
Standard Deviation 148.81 199.41 210.80 237.90 161.75 174.72 176.35 235.41 157.31 208.61 192.06 236.56
Intensity Change Log 2 — 0.357269 0.494632 0.685550 — 0.049215 0.420736 0.615353 — 0.208350 0.457412 0.650170

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002545 0.014495 0.035366 0.029973 0.006038 0.007523 0.018641 0.017883 0.004507 0.010896 0.025843 0.023079
Bud Neck 0.057158 0.067360 0.093144 0.055533 0.093822 0.068026 0.066709 0.081546 0.077756 0.067704 0.078092 0.070368
Bud Site 0.174775 0.288809 0.314451 0.361733 0.246708 0.215663 0.292285 0.250805 0.215187 0.251049 0.301830 0.298475
Cell Periphery 0.518968 0.244458 0.178905 0.145740 0.112269 0.204020 0.115590 0.213838 0.290484 0.223583 0.142854 0.184574
Cytoplasm 0.213401 0.319654 0.252820 0.284542 0.462461 0.421964 0.407507 0.359827 0.353324 0.372469 0.340897 0.327474
Cytoplasmic Foci 0.003534 0.006474 0.007662 0.021418 0.024150 0.015782 0.009682 0.009378 0.015116 0.011279 0.008812 0.014552
Eisosomes 0.001009 0.001789 0.003528 0.001247 0.001486 0.002151 0.001191 0.001593 0.001277 0.001976 0.002197 0.001445
Endoplasmic Reticulum 0.012540 0.013631 0.015170 0.001894 0.005905 0.012800 0.002233 0.001817 0.008812 0.013202 0.007804 0.001850
Endosome 0.001298 0.002574 0.002419 0.005163 0.006436 0.005509 0.002103 0.001584 0.004185 0.004089 0.002239 0.003122
Golgi 0.000867 0.001978 0.001538 0.004772 0.003618 0.005361 0.000653 0.002794 0.002413 0.003725 0.001034 0.003644
Lipid Particles 0.000516 0.004088 0.016577 0.015565 0.003327 0.006546 0.006946 0.012650 0.002095 0.005357 0.011093 0.013902
Mitochondria 0.002316 0.001524 0.000382 0.001930 0.000646 0.002779 0.000341 0.000601 0.001378 0.002172 0.000359 0.001173
Mitotic Spindle 0.000236 0.000157 0.000651 0.003456 0.000112 0.000207 0.000657 0.000290 0.000166 0.000183 0.000655 0.001651
None 0.000040 0.000047 0.000184 0.000072 0.000251 0.000154 0.000050 0.000201 0.000158 0.000102 0.000108 0.000146
Nuclear Periphery 0.000113 0.000108 0.000171 0.000126 0.000052 0.000116 0.000061 0.000101 0.000079 0.000112 0.000108 0.000112
Nuclear Periphery Foci 0.000298 0.000733 0.002358 0.001280 0.000640 0.000483 0.000940 0.000828 0.000490 0.000604 0.001550 0.001022
Nucleolus 0.000047 0.000043 0.000062 0.000049 0.000086 0.000292 0.000037 0.000048 0.000069 0.000171 0.000048 0.000048
Nucleus 0.000585 0.001385 0.010174 0.002315 0.002414 0.003118 0.003628 0.002496 0.001612 0.002280 0.006447 0.002418
Peroxisomes 0.002667 0.002298 0.000952 0.003185 0.002456 0.003788 0.001333 0.000933 0.002548 0.003067 0.001169 0.001901
Vacuole 0.005349 0.027496 0.062941 0.057233 0.025299 0.021337 0.069075 0.039719 0.016557 0.024316 0.066434 0.047245
Vacuole Periphery 0.001738 0.000900 0.000544 0.002771 0.001824 0.002380 0.000340 0.001068 0.001786 0.001664 0.000428 0.001800

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -13.02 -18.58 -14.05 -7.13 2.32 -2.68 -13.67 -8.19 -7.22 0.33 -11.86 -22.78 -15.68 -9.67 2.00
Bud Neck -2.08 -6.54 0.77 3.30 8.30 4.72 5.43 2.23 -2.80 -3.56 2.68 0.31 2.22 -0.53 2.21
Bud Site -10.84 -13.49 -16.05 -5.75 -3.92 3.18 -4.25 -0.50 -3.84 3.91 -4.97 -11.70 -10.95 -6.10 0.22
Cell Periphery 22.57 29.75 32.15 11.29 4.19 -11.32 -0.08 -13.14 -1.00 -13.46 8.31 20.27 13.96 6.58 -7.34
Cytoplasm -10.14 -4.01 -6.85 2.90 -3.32 3.54 4.87 9.12 5.77 5.01 -2.32 1.30 2.93 5.63 1.88
Cytoplasmic Foci -2.92 -5.02 -6.67 -5.37 -4.88 3.55 6.52 5.48 2.68 0.22 2.71 4.45 -0.26 -2.41 -3.65
Eisosomes -3.88 -8.57 -1.10 3.87 8.88 -3.54 2.25 -0.58 2.82 -2.75 -5.13 -6.01 -1.15 4.27 5.25
Endoplasmic Reticulum -0.54 -1.57 7.27 7.80 9.91 -3.93 3.88 3.96 6.79 0.52 -3.35 0.88 8.04 10.23 8.59
Endosome -3.11 -3.70 -4.70 -2.84 -3.08 1.14 6.71 6.72 5.45 1.18 0.22 4.88 2.03 1.80 -1.76
Golgi -2.70 -4.27 -3.07 -2.04 -2.52 -1.87 7.89 0.88 2.11 -2.42 -2.48 6.22 -1.50 0.22 -3.49
Lipid Particles -5.30 -12.46 -7.72 -5.66 0.24 -2.94 -4.16 -6.01 -3.43 -3.43 -4.95 -12.06 -9.70 -6.46 -2.17
Mitochondria 1.35 7.21 0.75 -0.45 -2.75 -3.97 2.70 0.29 3.93 -1.49 -2.02 7.55 0.85 2.30 -3.11
Mitotic Spindle 3.12 -1.17 -3.17 -3.24 -2.62 -1.93 -1.34 -2.83 -1.10 0.86 -0.61 -1.77 -3.38 -3.34 -2.00
None -0.40 -6.54 -2.61 -1.32 3.97 0.92 2.02 0.31 -0.44 -1.42 0.94 0.88 0.09 -0.74 -0.67
Nuclear Periphery 0.50 -4.73 -0.62 -0.83 1.58 -3.62 -1.27 -5.96 0.78 -5.50 -3.07 -4.44 -2.84 -0.14 -0.39
Nuclear Periphery Foci -8.21 -12.50 -7.26 -4.32 4.48 1.61 -2.55 -1.73 -5.44 1.30 -1.77 -10.86 -6.54 -6.17 4.54
Nucleolus 1.02 -1.46 -0.42 -0.67 0.46 -1.42 3.15 2.38 1.72 -1.80 -1.37 2.15 1.66 1.64 -0.25
Nucleus -8.73 -18.87 -9.01 -5.48 13.52 -0.78 -2.82 -0.34 0.67 4.55 -1.38 -14.29 -3.61 -0.87 13.68
Peroxisomes 1.12 6.63 -0.68 -1.19 -3.15 -3.24 3.32 4.53 10.88 3.00 -1.92 6.23 1.75 3.37 -2.30
Vacuole -16.16 -23.91 -16.96 -9.98 0.83 2.11 -15.03 -6.46 -8.40 9.84 -6.23 -25.29 -16.95 -12.83 7.70
Vacuole Periphery 3.44 4.77 -1.31 -2.58 -3.10 -0.71 2.33 1.01 2.13 -1.79 0.27 3.64 0.07 -0.19 -3.54
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Glucose-repressible ADP-ribosylation factor; GTPase of Ras superfamily involved in regulating cell polarity and invasive growth; localizes to dynamic spots at plasma membrane and modulates PtdIns(4,5)P2 levels to facilitate endocytosis; required for localization of endocytic protein Lsb5p to correct cortical site in cells; also has mRNA binding activity; homolog of mammalian Arf6
Localization
Cell Percentages cell periphery (33%), cytoplasm (14%), mixed (38%)
Cell Cycle Regulation Yes
cytoplasm - MA (0.00047)
Subcompartmental Group N/A

Arf3

Arf3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arf3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available