Standard name
Human Ortholog
Description Glucose-repressible ADP-ribosylation factor; GTPase of Ras superfamily involved in regulating cell polarity and invasive growth; localizes to dynamic spots at plasma membrane and modulates PtdIns(4,5)P2 levels to facilitate endocytosis; required for localization of endocytic protein Lsb5p to correct cortical site in cells; also has mRNA binding activity; homolog of mammalian Arf6

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.3 0.25 0.34 0.14 0.28 0.21 0.15 0.1 0.08 0.12 0.06 0.08 0.3 0.29 0.17 0.2 0.14 0.08 0.14 0.1 0.06 0.1 0.08
Bud 0.12 0.14 0.22 0.1 0.19 0.16 0.13 0.12 0.12 0.12 0.1 0.11 0.12 0.07 0.11 0.13 0.08 0.06 0.05 0.05 0 0.07 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.17 0.26 0.17 0.16 0.11 0.14
Bud Site 0.07 0.06 0.06 0 0.06 0 0 0 0 0 0 0 0.06 0.05 0.09 0.07 0
Cell Periphery 0.65 0.52 0.52 0.53 0.28 0.46 0.37 0.25 0.43 0.32 0.38 0.37 0.51 0.5 0.12 0.19 0.22 0.52 0.31 0.5 0.51 0.45 0.42
Cytoplasm 0.35 0.45 0.33 0.58 0.48 0.53 0.59 0.57 0.65 0.65 0.7 0.63 0.38 0.37 0.36 0.26 0.34 0.06 0.14 0.06 0.09 0.09 0.15
Endoplasmic Reticulum 0 0 0 0.06 0.08 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.08 0 0.14 0.09
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.07 0.09 0.09 0.1 0.1 0.14 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.12 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
SpindlePole 0 0 0 0.06 0 0 0 0 0 0 0 0.06 0 0.09 0.27 0.31 0.3 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0.05 0.05 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 77 49 50 27 84 71 80 52 32 65 27 43 31 48 45 46 31 25 35 15 3 8 8
Bud 31 27 32 19 55 52 69 65 45 60 44 56 12 12 31 29 17 17 13 8 0 5 6
Bud Neck 1 0 0 1 1 0 0 0 0 0 0 0 0 0 1 7 0 53 65 26 9 9 16
Bud Site 18 11 9 6 17 9 17 5 2 8 6 8 6 9 23 16 10
Cell Periphery 168 103 77 102 84 154 197 131 166 165 160 198 52 82 33 42 49 162 78 81 29 38 48
Cytoplasm 91 90 49 112 141 178 315 299 248 341 294 334 39 60 97 58 76 18 35 8 5 7 17
Endoplasmic Reticulum 9 7 5 12 23 21 34 10 14 11 8 10 2 5 12 5 8 26 17 12 2 11 9
Endosome 0 1 1 5 6 0 3 11 2 7 11 4 0 9 7 11 12 1 1 1 0 0 0
Golgi 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 1 0 1 0 0
Mitochondria 0 0 2 0 1 13 38 46 35 53 42 73 0 0 10 6 6 1 0 1 1 1 0
Nucleus 0 2 0 0 1 1 3 6 1 2 1 2 0 1 9 5 3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 2 17 1 1 3 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 3 0 2 1 3 23 5 6 7 15 1 3 24 26 12 0 0 0 0 0 0
Peroxisomes 0 1 0 2 0 1 0 2 2 1 3 6 1 0 5 6 12 0 0 0 0 0 0
SpindlePole 2 3 6 11 11 2 5 10 6 19 14 30 3 14 74 69 68 0 0 2 0 0 2
Vac/Vac Membrane 0 2 3 4 8 3 23 29 16 28 16 13 0 8 14 14 10 2 3 0 2 0 0
Unique Cell Count 259 199 148 194 295 334 538 520 382 521 423 529 102 164 270 225 223 312 255 161 57 85 114
Labelled Cell Count 400 296 237 301 434 506 789 706 575 767 636 793 147 251 385 343 314 312 255 161 57 85 114


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.8 13.0 9.4 9.3 9.8 9.2 8.8 8.9 9.2 8.8 8.8 9.4 11.5 11.5 12.5 13.5 13.3 12.9 13.0 13.3
Std Deviation (1e-4) 2.4 2.3 1.8 1.9 2.0 1.8 2.0 2.1 2.2 2.1 2.1 2.4 1.7 2.0 3.7 3.9 3.8 2.4 2.4 2.4
Intensity Change (Log2) -0.02 0.06 -0.02 -0.09 -0.07 -0.02 -0.09 -0.1 0.01 0.3 0.3 0.42 0.53 0.5 0.46 0.48 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -4.4 -1.1 -2.9 -5.2 -7.1 -7.3 -6.1 -8.4 -8.0 -0.6 -0.9 -4.0 -2.9 -4.5
Bud -3.0 -0.7 -1.6 -2.7 -2.8 -2.9 -3.2 -3.5 -3.5 -2.0 -3.6 -2.8 -2.2 -3.9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site -1.3 -0.1 -1.8 -1.6 0 0 0 0 0 -0.1 -0.2 0.9 0.4 -0.7
Cell Periphery 0.1 -4.9 -1.2 -3.4 -6.2 -1.8 -4.5 -3.0 -3.2 -0.2 -0.4 -8.8 -6.8 -6.0
Cytoplasm 4.5 2.9 4.1 5.5 5.2 6.6 7.0 7.8 6.5 0.8 0.6 0.6 -1.5 0.2
Endoplasmic Reticulum 1.2 1.8 1.3 1.4 0 0.2 0 0 0 0 0 0.5 0 0.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 2.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.6 3.1 3.2 3.4 3.4 4.3 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1.3 0 0 0 0 0 0 2.7 3.4 1.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.7 -0.2 0 0 0 0 -0.2 -0.4 0.8 0 1.6 5.8 6.3 6.2
Vacuole 0 0 0 1.3 1.8 1.2 1.7 0 0 0 1.4 1.6 1.9 1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.473 7.1773 6.4071 5.7559 5.9601 6.7164 7.5676 7.1462 6.5656 7.3165 5.9159 6.9241 6.4026 5.6983 5.6598 5.9469 5.3652 5.3707
Actin 0.0474 0.0066 0.0274 0.1195 0.0182 0.0215 0.0403 0.0082 0.0478 0.0586 0.0654 0.0069 0.0651 0.0089 0.0119 0.0066 0.0065 0.0193
Bud 0.0029 0.0055 0.0173 0.0058 0.0063 0.0034 0.0009 0.0014 0.0107 0.0043 0.0017 0.0024 0.0007 0.0028 0.0082 0.0112 0.0011 0.0011
Bud Neck 0.0169 0.0124 0.0604 0.0668 0.0614 0.1334 0.0111 0.0062 0.0429 0.0486 0.0132 0.1107 0.0152 0.0172 0.0511 0.0797 0.0838 0.1247
Bud Periphery 0.017 0.027 0.1845 0.0884 0.1146 0.0461 0.0045 0.005 0.1039 0.0907 0.0225 0.0279 0.0033 0.0084 0.0965 0.1756 0.019 0.019
Bud Site 0.0503 0.3667 0.1673 0.0158 0.0269 0.0083 0.0468 0.3875 0.1383 0.0192 0.0086 0.0074 0.0522 0.3899 0.1599 0.0047 0.0122 0.0019
Cell Periphery 0.5028 0.2952 0.3666 0.3657 0.5146 0.5096 0.5955 0.4151 0.4956 0.5852 0.5274 0.6276 0.5088 0.3439 0.5082 0.5498 0.4635 0.5225
Cytoplasm 0.2217 0.2598 0.0998 0.1498 0.0803 0.2244 0.1956 0.1647 0.0926 0.1628 0.1994 0.1807 0.2674 0.2009 0.1327 0.1379 0.2796 0.2762
Cytoplasmic Foci 0.0153 0.0005 0.0117 0.0232 0.0298 0.0031 0.0109 0.0002 0.0166 0.0026 0.0213 0.0061 0.012 0.0005 0.0008 0.0001 0.0045 0.0017
Eisosomes 0.0026 0.0006 0.003 0.0168 0.0005 0.0014 0.0025 0.0006 0.0008 0.0006 0.0081 0.0011 0.002 0.0042 0.0032 0.0001 0.0006 0.0005
Endoplasmic Reticulum 0.0292 0.0135 0.0102 0.018 0.0196 0.0158 0.0137 0.0038 0.0032 0.0047 0.0133 0.0094 0.0251 0.0077 0.0079 0.0197 0.0291 0.0148
Endosome 0.017 0.0006 0.0038 0.0167 0.0157 0.0055 0.0203 0 0.0021 0.0006 0.0161 0.0021 0.0094 0.0003 0.001 0 0.0104 0.001
Golgi 0.0089 0.0004 0.0034 0.0197 0.0087 0.0042 0.0065 0.0001 0.0045 0.0029 0.0388 0.0019 0.0073 0.0003 0.0006 0.0001 0.0107 0.0023
Lipid Particles 0.0105 0.0019 0.0021 0.0235 0.0207 0.0023 0.0081 0.0007 0.0068 0.002 0.018 0.0048 0.0034 0.0019 0.0017 0.0015 0.0393 0.0036
Mitochondria 0.0041 0.0001 0.0124 0.023 0.0055 0.0018 0.005 0 0.0007 0.0008 0.0058 0.0008 0.0008 0.0003 0.0001 0 0.0235 0.0017
None 0.0091 0.0004 0.0014 0.0013 0.0023 0.0023 0.0118 0.0006 0.0054 0.0011 0.0023 0.0011 0.0076 0.0008 0.002 0.0001 0.0005 0.0013
Nuclear Periphery 0.0097 0.0013 0.0018 0.0016 0.0028 0.0033 0.0101 0.0006 0.0017 0.0004 0.0011 0.0005 0.0087 0.001 0.0012 0.0009 0.0013 0.0004
Nucleolus 0.0014 0.0001 0.0035 0.0028 0.0009 0.0004 0.0005 0.0002 0.0011 0.0002 0.0001 0.0013 0.0002 0.0008 0.0002 0.0001 0.0003 0.0001
Nucleus 0.0079 0.0009 0.0028 0.0018 0.0015 0.0026 0.0033 0.0007 0.0019 0.0007 0.0004 0.0007 0.001 0.0009 0.0012 0.0006 0.0005 0.0006
Peroxisomes 0.0059 0 0.0095 0.0205 0.051 0.0012 0.0053 0 0.0103 0.0023 0.0327 0.0009 0.0015 0.0001 0 0 0.005 0.0023
Punctate Nuclear 0.0053 0.0001 0.0007 0.0007 0.0068 0.0006 0.0023 0.0001 0.0077 0.0001 0.0005 0.0004 0.0011 0.0001 0.0002 0 0.0003 0.0002
Vacuole 0.0128 0.0062 0.0099 0.0151 0.0115 0.0075 0.0046 0.0043 0.0052 0.0113 0.0031 0.0054 0.0067 0.009 0.0111 0.011 0.0078 0.0047
Vacuole Periphery 0.0011 0.0002 0.0004 0.0034 0.0003 0.0011 0.0007 0 0.0001 0.0001 0.0002 0.0001 0.0006 0.0001 0.0002 0.0001 0.0009 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.9924 32.1644 18.5635 20.5625 12.9584 19.9781 17.6158 18.2915 19.096 17.9601
Translational Efficiency 2.1994 1.2518 1.7294 2.2528 2.6976 1.4791 2.0592 2.102 2.0876 1.7182

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Glucose-repressible ADP-ribosylation factor; GTPase of Ras superfamily involved in regulating cell polarity and invasive growth; localizes to dynamic spots at plasma membrane and modulates PtdIns(4,5)P2 levels to facilitate endocytosis; required for localization of endocytic protein Lsb5p to correct cortical site in cells; also has mRNA binding activity; homolog of mammalian Arf6
Localization
Cell Percentages cell periphery (33%), cytoplasm (14%), mixed (38%)
Cell Cycle Regulation Yes
cytoplasm - MA (0.00047)
Subcompartmental Group N/A

Arf3

Arf3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arf3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available