Standard name | |
---|---|
Human Ortholog | |
Description | Alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family; relocalizes from vacuole to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 3 | 0 | 3 | 4 | 3 | 9 | 2 | 4 | 5 | 9 | 1 | 2 | 1 | 0 | 0 | 0 | 1 | 3 | 1 | 1 | 4 | 0 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 44 | 60 | 143 | 150 | 185 | 275 | 375 | 371 | 386 | 408 | 403 | 507 | 141 | 299 | 402 | 16 | 15 | 35 | 10 | 5 | 5 | 5 | 3 | 5 |
Endoplasmic Reticulum | 3 | 1 | 1 | 0 | 0 | 3 | 4 | 4 | 1 | 0 | 1 | 1 | 9 | 1 | 3 | 4 | 7 | 8 | 0 | 0 | 2 | 2 | 6 | 3 |
Endosome | 25 | 10 | 42 | 8 | 22 | 2 | 4 | 2 | 2 | 3 | 1 | 2 | 5 | 4 | 2 | 3 | 2 | 4 | 28 | 6 | 27 | 11 | 29 | 21 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 1 |
Mitochondria | 17 | 5 | 81 | 8 | 31 | 74 | 119 | 135 | 100 | 104 | 104 | 140 | 0 | 2 | 0 | 3 | 2 | 2 | 3 | 1 | 7 | 3 | 9 | 6 |
Nucleus | 74 | 22 | 39 | 18 | 36 | 118 | 83 | 97 | 54 | 81 | 55 | 54 | 14 | 12 | 4 | 58 | 45 | 69 | 6 | 3 | 3 | 1 | 4 | 4 |
Nuclear Periphery | 1 | 0 | 2 | 2 | 2 | 5 | 13 | 10 | 5 | 6 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 4 | 4 |
Nucleolus | 0 | 0 | 1 | 0 | 2 | 1 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 3 | 2 | 1 | 1 | 0 | 1 | 0 |
Vac/Vac Membrane | 332 | 173 | 279 | 198 | 318 | 400 | 438 | 431 | 415 | 443 | 288 | 360 | 115 | 137 | 64 | 60 | 80 | 81 | 377 | 215 | 448 | 222 | 299 | 302 |
Unique Cell Count | 412 | 219 | 467 | 286 | 479 | 655 | 783 | 838 | 722 | 814 | 682 | 833 | 246 | 401 | 443 | 115 | 122 | 162 | 436 | 243 | 506 | 255 | 370 | 357 |
Labelled Cell Count | 497 | 271 | 595 | 386 | 600 | 882 | 1041 | 1062 | 966 | 1050 | 860 | 1080 | 286 | 457 | 476 | 146 | 154 | 204 | 436 | 243 | 506 | 255 | 370 | 357 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.3 | 5.7 | 4.7 | 5.3 | 5.6 | 5.3 | 4.8 | 4.8 | 5.0 | 4.9 | 5.0 | 5.0 | 5.9 | 5.2 | 5.2 | 7.6 | 8.5 | 8.8 | 7.4 | 7.1 | 7.6 |
Std Deviation (1e-4) | 1.1 | 1.1 | 1.4 | 1.5 | 1.5 | 1.4 | 1.6 | 1.3 | 1.4 | 1.4 | 1.4 | 1.3 | 1.2 | 1.1 | 1.2 | 2.0 | 2.5 | 3.5 | 1.7 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.15 | 0.25 | 0.17 | 0.03 | 0.01 | 0.09 | 0.05 | 0.09 | 0.07 | 0.33 | 0.14 | 0.14 | 0.67 | 0.84 | 0.89 | 0.65 | 0.59 | 0.68 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6.0 | 2.6 | 3.9 | 6.0 | 4.8 | 7.7 | 6.8 | 9.5 | 10.5 | 6.9 | 12.9 | 18.5 | -3.6 | -4.1 | -2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -3.3 | -2.7 | -7.4 | 0 | 0 | -7.8 | -8.1 | -7.8 | -8.4 | -3.6 | -5.2 | -6.0 | -2.3 | -2.8 | -2.7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -6.0 | -5.2 | -2.9 | 0 | 0 | -1.6 | -2.2 | -0.9 | -0.2 | -6.9 | -8.4 | -9.2 | -4.0 | -4.4 | -5.2 |
Nucleus | -1.0 | -0.5 | 4.6 | 1.3 | 1.8 | -0.5 | 0.9 | -0.2 | -1.3 | -1.3 | -3.3 | -5.3 | 10.8 | 8.0 | 10.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.6 | 2.1 | 0.4 | -1.3 | -2.9 | -0.8 | -1.8 | -5.8 | -5.7 | -3.3 | -7.5 | -14.1 | -1.5 | 1.2 | -2.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.5835 | 3.2603 | 3.0512 | 2.5571 | 2.3751 | 3.1471 | 4.2857 | 6.4383 | 5.6198 | 5.3869 | 5.3809 | 5.9008 | 2.2998 | 5.0165 | 3.7655 | 3.8261 | 3.8496 | 4.0488 |
Actin | 0.0043 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0058 | 0 | 0.0005 | 0 | 0 | 0.0003 | 0.0082 | 0 | 0.0033 | 0 | 0 | 0 |
Bud | 0.0006 | 0.0005 | 0.0004 | 0.0002 | 0.0014 | 0.0007 | 0.0006 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0002 |
Bud Neck | 0.0031 | 0.0013 | 0.0022 | 0.0004 | 0.0014 | 0.0013 | 0.001 | 0.0008 | 0.0012 | 0.0008 | 0.0008 | 0.0016 | 0.0008 | 0.0006 | 0.001 | 0.001 | 0.0012 | 0.0011 |
Bud Periphery | 0.0015 | 0.0013 | 0.0009 | 0.0007 | 0.0053 | 0.0015 | 0.0018 | 0.001 | 0.0016 | 0.0013 | 0.0006 | 0.0019 | 0.0008 | 0.0006 | 0.0007 | 0.0006 | 0.0006 | 0.0012 |
Bud Site | 0.0082 | 0.0012 | 0.0021 | 0.0003 | 0.0011 | 0.0006 | 0.0012 | 0.0005 | 0.001 | 0.0005 | 0.0003 | 0.0006 | 0.0005 | 0.0005 | 0.0004 | 0.0004 | 0.0009 | 0.0007 |
Cell Periphery | 0.0024 | 0.0032 | 0.0039 | 0.0021 | 0.0036 | 0.0039 | 0.0043 | 0.0073 | 0.005 | 0.0038 | 0.0044 | 0.0071 | 0.0033 | 0.0037 | 0.0032 | 0.004 | 0.0052 | 0.0055 |
Cytoplasm | 0.0041 | 0.0041 | 0.0088 | 0.0011 | 0.0037 | 0.0016 | 0.0035 | 0.0017 | 0.0026 | 0.0005 | 0.0006 | 0.0014 | 0.0021 | 0.0039 | 0.0007 | 0.0004 | 0.0005 | 0.0007 |
Cytoplasmic Foci | 0.0315 | 0.0092 | 0.0184 | 0.0073 | 0.0117 | 0.0107 | 0.0124 | 0.004 | 0.0069 | 0.0008 | 0.0085 | 0.0041 | 0.005 | 0.0025 | 0.0031 | 0.0005 | 0.0025 | 0.0011 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0043 | 0.0026 | 0.0083 | 0.0005 | 0.0034 | 0.0015 | 0.0025 | 0.004 | 0.0037 | 0.0007 | 0.0017 | 0.002 | 0.0029 | 0.0029 | 0.0014 | 0.0007 | 0.0007 | 0.0018 |
Endosome | 0.2543 | 0.1085 | 0.2081 | 0.2043 | 0.2048 | 0.0857 | 0.1549 | 0.0995 | 0.1394 | 0.0815 | 0.0982 | 0.1077 | 0.1907 | 0.0969 | 0.1467 | 0.0592 | 0.1028 | 0.0734 |
Golgi | 0.0285 | 0.0032 | 0.0087 | 0.0059 | 0.008 | 0.0045 | 0.0078 | 0.0043 | 0.0097 | 0.0022 | 0.0045 | 0.0059 | 0.0077 | 0.0036 | 0.0084 | 0.0011 | 0.0044 | 0.0024 |
Lipid Particles | 0.0598 | 0.0119 | 0.0121 | 0.0055 | 0.0135 | 0.0182 | 0.0224 | 0.0169 | 0.0322 | 0.0062 | 0.0144 | 0.0209 | 0.0351 | 0.0076 | 0.0167 | 0.0029 | 0.0151 | 0.0103 |
Mitochondria | 0.0385 | 0.003 | 0.0191 | 0.0032 | 0.0044 | 0.0182 | 0.0133 | 0.0125 | 0.0299 | 0.0147 | 0.0104 | 0.0123 | 0.0227 | 0.0138 | 0.0201 | 0.0012 | 0.0011 | 0.0057 |
None | 0.0177 | 0.0002 | 0.0009 | 0.0001 | 0.0032 | 0.0007 | 0.0126 | 0.0001 | 0.0032 | 0 | 0.0001 | 0.0013 | 0.0037 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 |
Nuclear Periphery | 0.0112 | 0.014 | 0.0109 | 0.0011 | 0.0025 | 0.0031 | 0.0065 | 0.0047 | 0.0045 | 0.001 | 0.0024 | 0.0038 | 0.0114 | 0.005 | 0.0023 | 0.0015 | 0.0012 | 0.0103 |
Nucleolus | 0.0041 | 0.0035 | 0.0055 | 0.0017 | 0.0055 | 0.004 | 0.0048 | 0.002 | 0.0052 | 0.0022 | 0.002 | 0.0028 | 0.0041 | 0.0021 | 0.0027 | 0.0031 | 0.0042 | 0.0046 |
Nucleus | 0.0038 | 0.006 | 0.0048 | 0.0017 | 0.0054 | 0.004 | 0.0038 | 0.0021 | 0.0029 | 0.002 | 0.0016 | 0.003 | 0.0031 | 0.0023 | 0.0018 | 0.002 | 0.0021 | 0.0042 |
Peroxisomes | 0.0182 | 0.0008 | 0.0026 | 0.0007 | 0.001 | 0.0013 | 0.0046 | 0.0004 | 0.007 | 0.0005 | 0.0011 | 0.0017 | 0.003 | 0.0002 | 0.0032 | 0.0002 | 0.0008 | 0.0004 |
Punctate Nuclear | 0.0021 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0008 | 0 | 0.0002 | 0.0018 | 0.0016 | 0.0001 | 0.0001 | 0 | 0 | 0.0007 |
Vacuole | 0.3533 | 0.6467 | 0.5191 | 0.5916 | 0.6059 | 0.6738 | 0.5222 | 0.6343 | 0.5546 | 0.6793 | 0.6621 | 0.624 | 0.4866 | 0.6147 | 0.5461 | 0.6981 | 0.6828 | 0.6528 |
Vacuole Periphery | 0.1485 | 0.1787 | 0.1627 | 0.1718 | 0.1139 | 0.1646 | 0.2129 | 0.2037 | 0.1878 | 0.2016 | 0.1861 | 0.1955 | 0.2061 | 0.2384 | 0.2378 | 0.2232 | 0.1737 | 0.2228 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 182.5005 | 141.0301 | 126.9428 | 119.9302 | 144.0295 | 160.4742 | 146.8142 | 131.5501 | 118.7571 | 163.5304 |
Translational Efficiency | 1.0532 | 1.1722 | 0.9706 | 1.1762 | 1.1865 | 1.0969 | 1.0876 | 1.063 | 0.9064 | 0.9833 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1480 | 1596 | 118 | 992 | 1920 | 124 | 136 | 1064 | 3400 | 1720 | 254 | 2056 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 669.45 | 842.70 | 922.14 | 907.98 | 748.92 | 806.90 | 963.42 | 959.22 | 714.33 | 840.12 | 944.24 | 934.50 |
Standard Deviation | 78.38 | 122.99 | 100.01 | 144.88 | 100.19 | 95.04 | 99.37 | 141.04 | 99.47 | 121.54 | 101.77 | 145.18 |
Intensity Change Log 2 | — | 0.332043 | 0.462009 | 0.439684 | — | 0.107578 | 0.363353 | 0.357050 | — | 0.217882 | 0.410759 | 0.396643 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000046 | 0.000045 | 0.001069 | 0.001669 | 0.000053 | 0.000083 | 0.000396 | 0.001950 | 0.000050 | 0.000048 | 0.000709 | 0.001815 |
Bud Neck | 0.029285 | 0.011398 | 0.006530 | 0.020374 | 0.014658 | 0.022558 | 0.016385 | 0.020572 | 0.021025 | 0.012202 | 0.011807 | 0.020477 |
Bud Site | 0.011077 | 0.010434 | 0.019528 | 0.053053 | 0.007908 | 0.005554 | 0.008377 | 0.050295 | 0.009287 | 0.010082 | 0.013557 | 0.051626 |
Cell Periphery | 0.000323 | 0.000374 | 0.000140 | 0.000142 | 0.000228 | 0.000976 | 0.000109 | 0.000159 | 0.000269 | 0.000417 | 0.000124 | 0.000151 |
Cytoplasm | 0.072855 | 0.024119 | 0.092772 | 0.099903 | 0.053399 | 0.017897 | 0.053581 | 0.105555* | 0.061868 | 0.023670 | 0.071788 | 0.102828* |
Cytoplasmic Foci | 0.064795 | 0.016519 | 0.139693 | 0.082409 | 0.033112 | 0.026158 | 0.047793 | 0.119265 | 0.046903 | 0.017214 | 0.090487 | 0.101482 |
Eisosomes | 0.000074 | 0.000016 | 0.000069 | 0.000024 | 0.000019 | 0.000067 | 0.000070 | 0.000033 | 0.000043 | 0.000019 | 0.000069 | 0.000029 |
Endoplasmic Reticulum | 0.004929 | 0.003206 | 0.008376 | 0.001114 | 0.001677 | 0.002087 | 0.004913 | 0.001562 | 0.003092 | 0.003125 | 0.006522 | 0.001346 |
Endosome | 0.219004* | 0.067061 | 0.239041* | 0.202207* | 0.091776 | 0.069290 | 0.279183* | 0.280627* | 0.147158 | 0.067222 | 0.260534* | 0.242790* |
Golgi | 0.002819 | 0.002208 | 0.003199 | 0.010564 | 0.001898 | 0.002115 | 0.001109 | 0.009671 | 0.002299 | 0.002201 | 0.002080 | 0.010102 |
Lipid Particles | 0.009636 | 0.001087 | 0.019338 | 0.002690 | 0.001692 | 0.013026 | 0.010708 | 0.003665 | 0.005150 | 0.001947 | 0.014717 | 0.003195 |
Mitochondria | 0.004486 | 0.001294 | 0.000408 | 0.005391 | 0.002154 | 0.018306 | 0.000358 | 0.003571 | 0.003169 | 0.002521 | 0.000381 | 0.004449 |
Mitotic Spindle | 0.000232 | 0.001086 | 0.013390 | 0.020831 | 0.000347 | 0.000297 | 0.018748 | 0.016732 | 0.000297 | 0.001029 | 0.016259 | 0.018710 |
None | 0.000381 | 0.000199 | 0.000478 | 0.000262 | 0.000167 | 0.000098 | 0.000262 | 0.000258 | 0.000260 | 0.000192 | 0.000362 | 0.000260 |
Nuclear Periphery | 0.000729 | 0.000180 | 0.001776 | 0.000396 | 0.000240 | 0.000993 | 0.002870 | 0.000454 | 0.000453 | 0.000239 | 0.002362 | 0.000426 |
Nuclear Periphery Foci | 0.000227 | 0.000174 | 0.018597 | 0.001396 | 0.000113 | 0.000334 | 0.015817 | 0.001930 | 0.000163 | 0.000186 | 0.017109 | 0.001672 |
Nucleolus | 0.001045 | 0.000652 | 0.002705 | 0.001136 | 0.000513 | 0.002658 | 0.010596 | 0.000953 | 0.000745 | 0.000796 | 0.006930 | 0.001041 |
Nucleus | 0.018750 | 0.003796 | 0.034067 | 0.006559 | 0.006701 | 0.011499 | 0.039258 | 0.005993 | 0.011946 | 0.004351 | 0.036846 | 0.006266 |
Peroxisomes | 0.003126 | 0.000948 | 0.016902 | 0.014787 | 0.001288 | 0.005764 | 0.015503 | 0.026301 | 0.002088 | 0.001295 | 0.016153 | 0.020746 |
Vacuole | 0.537312* | 0.811832* | 0.376977* | 0.465264* | 0.762193* | 0.784830* | 0.470036* | 0.344707* | 0.664304* | 0.809885* | 0.426804* | 0.402874* |
Vacuole Periphery | 0.018868 | 0.043374 | 0.004945 | 0.009828 | 0.019866 | 0.015408 | 0.003928 | 0.005747 | 0.019432 | 0.041358 | 0.004401 | 0.007716 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.10 | -2.06 | -2.46 | -2.46 | -0.50 | -0.86 | -3.31 | -3.75 | -3.68 | -2.77 | -0.00 | -2.88 | -4.31 | -4.31 | -2.00 |
Bud Neck | 8.29 | 10.45 | 3.62 | -5.30 | -8.05 | -1.18 | -0.21 | -2.76 | 0.38 | -1.36 | 6.30 | 4.88 | 0.57 | -5.60 | -4.41 |
Bud Site | 0.35 | -0.94 | -9.82 | -10.42 | -3.90 | 0.84 | -0.27 | -11.56 | -9.42 | -10.81 | -0.77 | -1.01 | -15.18 | -14.54 | -8.31 |
Cell Periphery | -1.23 | 5.25 | 5.28 | 5.64 | 0.37 | -3.25 | 3.67 | 2.60 | 3.68 | -0.67 | -3.69 | 6.10 | 5.28 | 7.20 | -0.17 |
Cytoplasm | 11.82 | -1.92 | -7.97 | -16.16 | -1.39 | 6.07 | -1.00 | -11.29 | -14.14 | -4.34 | 12.69 | -2.13 | -13.86 | -22.25 | -3.99 |
Cytoplasmic Foci | 15.56 | -5.13 | -2.55 | -15.38 | 4.24 | 0.74 | -2.36 | -16.38 | -8.83 | -6.98 | 13.48 | -5.41 | -14.05 | -24.25 | -0.18 |
Eisosomes | 10.67 | -0.10 | 8.88 | -3.32 | 6.26 | -2.64 | -6.58 | -4.70 | 1.85 | 4.82 | 7.47 | -5.20 | 4.71 | -3.59 | 7.67 |
Endoplasmic Reticulum | 2.93 | -1.17 | 7.13 | 5.22 | 2.56 | -1.24 | -4.16 | 0.82 | 1.69 | 4.32 | -0.10 | -2.68 | 6.17 | 5.23 | 4.01 |
Endosome | 22.02* | 0.23 | 6.05 | -12.90 | 2.20 | 1.66 | -6.26 | -15.44 | -10.11 | 0.71 | 17.78 | -4.89 | -7.48 | -21.20 | 1.99 |
Golgi | 0.99 | 0.03 | -3.12 | -3.51 | -3.04 | -0.24 | 2.42 | -4.31 | -3.44 | -5.15 | 0.19 | 1.21 | -5.21 | -5.09 | -5.62 |
Lipid Particles | 9.93 | -2.33 | 7.73 | -6.75 | 4.07 | -1.57 | -4.17 | -5.31 | 1.25 | 3.14 | 4.67 | -4.34 | 3.65 | -2.59 | 5.12 |
Mitochondria | 3.80 | 4.89 | 0.20 | -2.46 | -3.22 | -1.66 | 3.36 | -0.76 | 1.59 | -3.38 | 0.76 | 5.90 | -0.39 | -0.98 | -4.54 |
Mitotic Spindle | -1.45 | -2.33 | -7.36 | -6.99 | -1.60 | 0.18 | -1.82 | -7.29 | -7.31 | -0.34 | -1.33 | -2.72 | -10.34 | -9.70 | -1.13 |
None | 1.77 | -0.85 | 0.46 | -1.02 | 1.09 | 1.06 | -1.50 | -1.62 | -2.38 | 0.16 | 0.99 | -1.56 | -0.75 | -1.54 | 0.99 |
Nuclear Periphery | 5.11 | -2.75 | 0.22 | -6.18 | 2.81 | -1.59 | -4.11 | -2.99 | 0.72 | 3.60 | 2.52 | -4.97 | -2.34 | -5.76 | 4.56 |
Nuclear Periphery Foci | 1.25 | -4.74 | -5.66 | -5.80 | 4.33 | -3.06 | -4.34 | -5.01 | -4.58 | 3.66 | -0.65 | -6.42 | -7.13 | -7.03 | 5.64 |
Nucleolus | 2.09 | -1.59 | -1.41 | -3.14 | 1.41 | -1.30 | -2.60 | -2.98 | 0.94 | 2.43 | -0.42 | -3.02 | -3.30 | -1.92 | 2.78 |
Nucleus | 8.53 | -2.43 | 4.11 | -6.24 | 3.37 | -1.70 | -4.32 | -2.43 | 0.69 | 4.05 | 7.85 | -4.98 | 1.74 | -7.01 | 5.26 |
Peroxisomes | 5.35 | -2.91 | -6.09 | -7.73 | 0.80 | -2.34 | -3.04 | -11.28 | -5.99 | -1.89 | 2.71 | -4.23 | -12.71 | -13.32 | -0.82 |
Vacuole | -27.44 | 5.69 | 4.15 | 29.13* | -3.66 | -0.77 | 10.65 | 32.86 | 13.34 | 3.10 | -18.85 | 11.91 | 26.11 | 42.38* | -0.15 |
Vacuole Periphery | -11.75 | 9.28 | 8.83 | 17.28 | -2.24 | 1.28 | 16.67 | 14.37 | 3.17 | -1.51 | -11.70 | 17.31 | 16.20 | 19.18 | -2.75 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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