Standard name | |
---|---|
Human Ortholog | |
Description | Exomer-dependent cargo protein; induces appearance of [PIN+] prion when overproduced; prion-like domain serves as a retention signal in the trans-Golgi network; predicted to be palmitoylated |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 12 | 37 | 108 | 95 | 46 | 58 | 59 | 80 | 43 | 47 | 46 | 57 | 12 | 25 | 24 | 12 | 23 | 17 | 16 | 28 | 93 | 10 | 29 | 17 |
Bud | 3 | 26 | 39 | 39 | 16 | 28 | 45 | 56 | 47 | 48 | 32 | 47 | 4 | 6 | 8 | 1 | 1 | 3 | 9 | 23 | 41 | 11 | 22 | 11 |
Bud Neck | 0 | 0 | 7 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 16 | 54 | 13 | 24 | 10 |
Bud Site | 0 | 3 | 5 | 17 | 0 | 6 | 6 | 0 | 4 | 4 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 6 | 6 | 3 | 3 | 6 | 7 | 6 | 5 | 9 | 5 | 1 | 8 | 5 | 3 | 3 | 4 | 0 | 0 | 3 | 0 | 1 | 0 |
Cytoplasm | 6 | 59 | 98 | 157 | 211 | 283 | 434 | 416 | 301 | 430 | 256 | 345 | 17 | 88 | 105 | 38 | 17 | 31 | 3 | 15 | 17 | 11 | 24 | 31 |
Endoplasmic Reticulum | 9 | 28 | 62 | 19 | 22 | 38 | 20 | 30 | 22 | 31 | 37 | 24 | 16 | 26 | 26 | 7 | 7 | 11 | 13 | 19 | 57 | 7 | 30 | 32 |
Endosome | 0 | 0 | 4 | 14 | 31 | 54 | 75 | 87 | 31 | 64 | 22 | 68 | 1 | 2 | 7 | 37 | 31 | 18 | 4 | 12 | 27 | 6 | 5 | 6 |
Golgi | 0 | 1 | 2 | 1 | 2 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 15 | 32 | 22 | 4 | 4 | 12 | 2 | 0 | 2 |
Mitochondria | 1 | 33 | 0 | 6 | 0 | 2 | 5 | 6 | 36 | 28 | 24 | 47 | 3 | 0 | 2 | 16 | 8 | 7 | 4 | 18 | 26 | 5 | 5 | 7 |
Nucleus | 0 | 1 | 3 | 0 | 0 | 5 | 5 | 4 | 3 | 3 | 5 | 5 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 4 | 1 | 1 | 3 | 4 | 0 | 0 | 1 | 3 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 4 | 10 | 1 | 6 | 14 | 4 | 0 | 5 | 1 | 2 | 0 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 28 | 23 | 12 | 11 | 13 | 20 | 6 | 13 | 5 | 11 | 0 | 3 | 7 | 12 | 10 | 18 | 0 | 0 | 2 | 0 | 0 | 0 |
Vac/Vac Membrane | 3 | 8 | 30 | 15 | 103 | 81 | 120 | 159 | 77 | 104 | 53 | 94 | 0 | 0 | 0 | 62 | 55 | 60 | 25 | 64 | 112 | 20 | 60 | 68 |
Unique Cell Count | 26 | 156 | 311 | 319 | 386 | 488 | 689 | 750 | 490 | 674 | 422 | 584 | 44 | 135 | 165 | 169 | 150 | 150 | 87 | 210 | 465 | 94 | 208 | 196 |
Labelled Cell Count | 34 | 197 | 396 | 404 | 448 | 577 | 806 | 877 | 577 | 785 | 494 | 712 | 54 | 162 | 187 | 207 | 190 | 197 | 87 | 210 | 465 | 94 | 208 | 196 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.9 | 5.7 | 8.3 | 7.7 | 8.4 | 7.5 | 7.7 | 7.9 | 6.6 | 7.4 | 7.3 | 7.3 | 6.8 | 7.4 | 6.4 | 8.4 | 10.4 | 11.6 | 10.6 | 10.8 | 10.6 |
Std Deviation (1e-4) | 1.4 | 0.9 | 1.4 | 1.5 | 1.4 | 1.4 | 1.4 | 1.2 | 1.2 | 1.2 | 1.2 | 1.3 | 1.2 | 3.1 | 1.4 | 1.4 | 1.8 | 2.6 | 8.1 | 8.4 | 7.8 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.03 | -0.14 | -0.11 | -0.08 | -0.32 | -0.16 | -0.19 | -0.18 | -0.3 | -0.17 | -0.37 | 0.02 | 0.33 | 0.48 | 0.35 | 0.39 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.3 | -7.2 | -7.8 | -10.3 | -9.3 | -9.2 | -11.1 | -7.8 | -9.2 | -1.0 | -3.4 | -4.7 | -6.7 | -4.3 | -5.3 |
Bud | -0.1 | -4.1 | -3.4 | -3.2 | -2.6 | -1.3 | -2.8 | -2.2 | -2.2 | -0.7 | -2.6 | -2.7 | -4.5 | -4.2 | -3.7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4.5 | 6.1 | 7.3 | 9.2 | 7.1 | 8.3 | 9.4 | 7.8 | 7.9 | 0.9 | 6.6 | 6.7 | -2.1 | -4.7 | -2.4 |
Endoplasmic Reticulum | -5.2 | -5.7 | -5.1 | -9.1 | -8.4 | -7.0 | -7.7 | -4.4 | -7.6 | 2.5 | -0.2 | -1.1 | -4.7 | -4.3 | -3.5 |
Endosome | 2.3 | 4.1 | 5.2 | 5.2 | 5.5 | 3.4 | 4.7 | 2.8 | 5.4 | 0 | 0 | 0 | 7.7 | 7.4 | 5.1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.7 | 8.0 | 6.4 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4.9 | 3.6 | 4.3 | 5.1 | 0 | 0 | 0 | 5.5 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1.6 | 0 | 0 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -0.8 | -3.3 | -4.3 | -5.3 | -4.5 | -5.3 | -5.2 | -5.0 | -5.0 | 0 | -2.6 | -1.9 | -0.7 | -0.9 | 1.0 |
Vacuole | -2.4 | 5.7 | 2.8 | 3.2 | 4.5 | 2.5 | 2.5 | 1.2 | 2.7 | 0 | -3.7 | -4.1 | 7.2 | 7.0 | 7.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.4634 | 6.1182 | 5.5416 | 5.4354 | 5.0954 | 6.0426 | 5.481 | 6.9288 | 6.011 | 6.2296 | 5.5214 | 6.6209 | 3.3537 | 3.9002 | 3.8501 | 3.7583 | 4.0424 | 4.1797 |
Actin | 0.122 | 0.0117 | 0.0826 | 0.0923 | 0.0464 | 0.0259 | 0.0612 | 0.0655 | 0.0699 | 0.093 | 0.1755 | 0.0374 | 0.0823 | 0.0123 | 0.0381 | 0.0479 | 0.0383 | 0.0238 |
Bud | 0.0289 | 0.0119 | 0.1687 | 0.0604 | 0.0301 | 0.0065 | 0.0154 | 0.0167 | 0.1056 | 0.0693 | 0.0468 | 0.0067 | 0.0448 | 0.0215 | 0.188 | 0.0868 | 0.0155 | 0.0035 |
Bud Neck | 0.0305 | 0.0075 | 0.0094 | 0.0132 | 0.0595 | 0.5829 | 0.0716 | 0.0169 | 0.0033 | 0.0059 | 0.0105 | 0.4514 | 0.0218 | 0.009 | 0.0049 | 0.0065 | 0.1525 | 0.4386 |
Bud Periphery | 0.0901 | 0.013 | 0.3865 | 0.3111 | 0.0962 | 0.0174 | 0.0533 | 0.0132 | 0.2092 | 0.2411 | 0.1526 | 0.0233 | 0.101 | 0.0445 | 0.3594 | 0.2387 | 0.1066 | 0.0096 |
Bud Site | 0.0997 | 0.3028 | 0.0498 | 0.0069 | 0.0172 | 0.0105 | 0.144 | 0.3257 | 0.0459 | 0.0105 | 0.0234 | 0.0098 | 0.0779 | 0.2235 | 0.0367 | 0.0245 | 0.0042 | 0.0042 |
Cell Periphery | 0.0404 | 0.0114 | 0.0059 | 0.0085 | 0.0068 | 0.0089 | 0.026 | 0.0073 | 0.0115 | 0.0094 | 0.018 | 0.0111 | 0.0171 | 0.0171 | 0.0055 | 0.0058 | 0.0115 | 0.0069 |
Cytoplasm | 0.298 | 0.3681 | 0.1177 | 0.247 | 0.1969 | 0.1526 | 0.1831 | 0.2757 | 0.1849 | 0.0997 | 0.2058 | 0.2738 | 0.3513 | 0.3139 | 0.1765 | 0.2049 | 0.316 | 0.2614 |
Cytoplasmic Foci | 0.0425 | 0.0556 | 0.0178 | 0.0131 | 0.0397 | 0.0364 | 0.0496 | 0.062 | 0.0496 | 0.0277 | 0.0535 | 0.0367 | 0.0309 | 0.0458 | 0.0178 | 0.0205 | 0.0592 | 0.0515 |
Eisosomes | 0.0014 | 0.0003 | 0.0002 | 0.0002 | 0.0011 | 0.0001 | 0.0035 | 0.0004 | 0.0005 | 0.0003 | 0.0007 | 0.0003 | 0.0007 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.026 | 0.0076 | 0.0039 | 0.0077 | 0.0108 | 0.0053 | 0.0141 | 0.0122 | 0.0069 | 0.0138 | 0.0071 | 0.0068 | 0.0229 | 0.0117 | 0.0045 | 0.0223 | 0.0052 | 0.015 |
Endosome | 0.0466 | 0.0987 | 0.0529 | 0.098 | 0.1262 | 0.0557 | 0.0954 | 0.1317 | 0.1612 | 0.1758 | 0.203 | 0.0692 | 0.0889 | 0.1213 | 0.0537 | 0.1311 | 0.149 | 0.0885 |
Golgi | 0.0281 | 0.0177 | 0.0203 | 0.0206 | 0.0894 | 0.054 | 0.0411 | 0.0427 | 0.0395 | 0.0498 | 0.042 | 0.0349 | 0.0276 | 0.0265 | 0.0088 | 0.0225 | 0.0253 | 0.0271 |
Lipid Particles | 0.0165 | 0.0059 | 0.0024 | 0.0034 | 0.0604 | 0.0058 | 0.0424 | 0.0026 | 0.0043 | 0.0352 | 0.0164 | 0.002 | 0.0137 | 0.0076 | 0.0017 | 0.0019 | 0.0285 | 0.0051 |
Mitochondria | 0.0115 | 0.0014 | 0.0077 | 0.0046 | 0.0412 | 0.0072 | 0.0588 | 0.0038 | 0.0271 | 0.017 | 0.0154 | 0.0045 | 0.0034 | 0.0027 | 0.0032 | 0.0067 | 0.0071 | 0.0042 |
None | 0.0499 | 0.0341 | 0.0044 | 0.0129 | 0.0572 | 0.0017 | 0.0677 | 0.0012 | 0.0238 | 0.0348 | 0.002 | 0.0043 | 0.0443 | 0.033 | 0.0109 | 0.0014 | 0.036 | 0.0129 |
Nuclear Periphery | 0.0076 | 0.0013 | 0.0015 | 0.0019 | 0.0039 | 0.0068 | 0.0107 | 0.0008 | 0.0019 | 0.0023 | 0.0012 | 0.0006 | 0.0115 | 0.0029 | 0.0018 | 0.0034 | 0.0008 | 0.0047 |
Nucleolus | 0.0009 | 0.0008 | 0.0005 | 0.0002 | 0.0011 | 0.0003 | 0.0016 | 0.0003 | 0.0008 | 0.001 | 0.0002 | 0.0002 | 0.0006 | 0.0014 | 0.0004 | 0.0005 | 0.0003 | 0.0002 |
Nucleus | 0.0027 | 0.0025 | 0.0014 | 0.0013 | 0.0014 | 0.0013 | 0.003 | 0.001 | 0.0017 | 0.0016 | 0.0007 | 0.0009 | 0.0051 | 0.0032 | 0.0024 | 0.0024 | 0.0013 | 0.0009 |
Peroxisomes | 0.0074 | 0.0015 | 0.0033 | 0.0003 | 0.0322 | 0.0018 | 0.0236 | 0.0026 | 0.0027 | 0.0047 | 0.0031 | 0.0013 | 0.0016 | 0.0017 | 0.0008 | 0.0013 | 0.0066 | 0.002 |
Punctate Nuclear | 0.0184 | 0.001 | 0.0009 | 0.0002 | 0.0081 | 0.0034 | 0.0059 | 0.0005 | 0.0009 | 0.0005 | 0.0007 | 0.0003 | 0.0015 | 0.0011 | 0.0013 | 0.0005 | 0.0006 | 0.0005 |
Vacuole | 0.0287 | 0.0419 | 0.0489 | 0.0763 | 0.0641 | 0.0131 | 0.0218 | 0.0152 | 0.0404 | 0.0927 | 0.0173 | 0.022 | 0.0472 | 0.0923 | 0.0725 | 0.1457 | 0.0308 | 0.0362 |
Vacuole Periphery | 0.0022 | 0.0031 | 0.0133 | 0.02 | 0.0101 | 0.0025 | 0.0061 | 0.0019 | 0.0088 | 0.0139 | 0.0041 | 0.0024 | 0.0039 | 0.007 | 0.0111 | 0.0247 | 0.0047 | 0.0031 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.2962 | 9.2945 | 18.1044 | 15.7045 | 11.7954 | 9.0925 | 20.851 | 22.5961 | 17.7959 | 16.0303 |
Translational Efficiency | 0.8347 | 1.935 | 1.0766 | 1.0672 | 1.0459 | 1.6672 | 0.8008 | 0.9265 | 1.2766 | 1.1218 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2116 | 493 | 1696 | 1376 | 1669 | 2403 | 2099 | 1584 | 3785 | 2896 | 3795 | 2960 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 859.25 | 923.95 | 1109.17 | 1185.13 | 793.24 | 1034.32 | 1105.74 | 1277.14 | 830.14 | 1015.53 | 1107.27 | 1234.37 |
Standard Deviation | 104.13 | 191.33 | 143.79 | 212.47 | 102.34 | 125.61 | 150.56 | 196.34 | 108.42 | 145.06 | 147.58 | 209.09 |
Intensity Change Log 2 | — | 0.104737 | 0.368331 | 0.463895 | — | 0.382853 | 0.479183 | 0.687087 | — | 0.244938 | 0.422607 | 0.575347 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.012642 | 0.034050 | 0.122804* | 0.085927 | 0.016109 | 0.030175 | 0.071717 | 0.070138 | 0.014171 | 0.030834 | 0.094548 | 0.077478 |
Bud Neck | 0.073797 | 0.038937 | 0.057549 | 0.056470 | 0.063125 | 0.070587 | 0.087750 | 0.048528 | 0.069091 | 0.065199 | 0.074253 | 0.052220 |
Bud Site | 0.241781* | 0.315434* | 0.523491* | 0.436752* | 0.189333* | 0.358474* | 0.574108* | 0.502679* | 0.218654* | 0.351147* | 0.551487* | 0.472032* |
Cell Periphery | 0.002598 | 0.010822 | 0.010277 | 0.172000* | 0.002555 | 0.004825 | 0.025262 | 0.122738* | 0.002579 | 0.005846 | 0.018565 | 0.145638* |
Cytoplasm | 0.206901* | 0.157566* | 0.095616 | 0.101122* | 0.213073* | 0.159963* | 0.115603* | 0.095770 | 0.209623* | 0.159555* | 0.106670* | 0.098258 |
Cytoplasmic Foci | 0.139756 | 0.184303* | 0.049378 | 0.015007 | 0.234273* | 0.117403 | 0.026945 | 0.019432 | 0.181433* | 0.128792 | 0.036971 | 0.017375 |
Eisosomes | 0.001215 | 0.000779 | 0.000947 | 0.004468 | 0.002329 | 0.001751 | 0.001089 | 0.002195 | 0.001706 | 0.001586 | 0.001026 | 0.003251 |
Endoplasmic Reticulum | 0.004936 | 0.005279 | 0.019200 | 0.007080 | 0.004959 | 0.003408 | 0.006508 | 0.007029 | 0.004946 | 0.003726 | 0.012180 | 0.007053 |
Endosome | 0.114721 | 0.105298 | 0.028215 | 0.027611 | 0.076247 | 0.079360 | 0.018091 | 0.032718 | 0.097756 | 0.083776 | 0.022616 | 0.030344 |
Golgi | 0.061557 | 0.061180 | 0.021555 | 0.016351 | 0.099386 | 0.083826 | 0.004207 | 0.020881 | 0.078238 | 0.079971 | 0.011960 | 0.018775 |
Lipid Particles | 0.008147 | 0.008927 | 0.015414 | 0.031386 | 0.017127 | 0.005920 | 0.008911 | 0.025283 | 0.012107 | 0.006432 | 0.011817 | 0.028120 |
Mitochondria | 0.019466 | 0.005703 | 0.004039 | 0.003033 | 0.020395 | 0.031852 | 0.001705 | 0.003134 | 0.019875 | 0.027400 | 0.002748 | 0.003087 |
Mitotic Spindle | 0.000276 | 0.000717 | 0.004359 | 0.001940 | 0.001593 | 0.000952 | 0.002485 | 0.003596 | 0.000857 | 0.000912 | 0.003323 | 0.002826 |
None | 0.000068 | 0.002035 | 0.000056 | 0.000189 | 0.000364 | 0.000107 | 0.000166 | 0.000052 | 0.000199 | 0.000435 | 0.000117 | 0.000116 |
Nuclear Periphery | 0.000021 | 0.000158 | 0.000054 | 0.000071 | 0.000041 | 0.000031 | 0.000060 | 0.000080 | 0.000030 | 0.000053 | 0.000057 | 0.000076 |
Nuclear Periphery Foci | 0.000108 | 0.000443 | 0.001046 | 0.000475 | 0.000267 | 0.000193 | 0.000749 | 0.000543 | 0.000178 | 0.000235 | 0.000881 | 0.000511 |
Nucleolus | 0.000163 | 0.000618 | 0.000040 | 0.000054 | 0.000337 | 0.000338 | 0.000046 | 0.000045 | 0.000239 | 0.000386 | 0.000043 | 0.000049 |
Nucleus | 0.001344 | 0.004093 | 0.003459 | 0.003981 | 0.003482 | 0.001158 | 0.003388 | 0.000958 | 0.002286 | 0.001658 | 0.003420 | 0.002363 |
Peroxisomes | 0.012599 | 0.011757 | 0.009842 | 0.005652 | 0.017109 | 0.014508 | 0.006468 | 0.004667 | 0.014588 | 0.014039 | 0.007976 | 0.005125 |
Vacuole | 0.083725 | 0.043058 | 0.030760 | 0.026563 | 0.031360 | 0.025731 | 0.043874 | 0.034530 | 0.060634 | 0.028681 | 0.038014 | 0.030827 |
Vacuole Periphery | 0.014180 | 0.008844 | 0.001898 | 0.003865 | 0.006538 | 0.009438 | 0.000867 | 0.005005 | 0.010810 | 0.009337 | 0.001328 | 0.004475 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.70 | -21.87 | -16.32 | -8.22 | 5.52 | -6.58 | -15.84 | -14.74 | -10.38 | 0.12 | -9.30 | -26.91 | -22.16 | -14.56 | 4.09 |
Bud Neck | 5.96 | 4.04 | 4.06 | -3.00 | 0.14 | -1.57 | -4.58 | 3.40 | 5.54 | 8.88 | 1.05 | -1.08 | 5.60 | 3.98 | 7.02 |
Bud Site | -4.84 | -28.11* | -17.80 | -7.20 | 6.81 | -18.87 | -39.83* | -29.73* | -13.54 | 5.70 | -18.25 | -47.76 | -33.27 | -14.05 | 8.89 |
Cell Periphery | -4.19 | -8.54 | -21.95 | -20.23 | -20.85 | -4.21 | -12.99 | -20.32 | -19.91 | -15.95 | -6.26 | -15.22 | -29.79 | -28.97 | -25.96 |
Cytoplasm | 5.09 | 17.47 | 15.52 | 5.53 | -0.74 | 7.91 | 13.79 | 17.16 | 11.30 | 3.97 | 9.98 | 21.72 | 23.33 | 12.68 | 2.45 |
Cytoplasmic Foci | -4.58 | 20.92 | 33.00 | 18.33 | 12.01 | 20.00 | 39.05 | 41.29 | 28.60 | 3.88 | 12.32 | 42.47 | 51.06 | 34.79 | 11.26 |
Eisosomes | 2.75 | 2.17 | -6.11 | -6.97 | -6.77 | 2.07 | 5.15 | 0.48 | -1.71 | -5.23 | 0.69 | 5.34 | -5.24 | -5.64 | -8.33 |
Endoplasmic Reticulum | -0.31 | -8.64 | -2.48 | -1.49 | 7.11 | 2.18 | -2.03 | -2.20 | -4.48 | -0.42 | 2.34 | -8.46 | -3.31 | -5.42 | 5.72 |
Endosome | 1.24 | 24.60 | 22.06 | 10.72 | 0.27 | -0.85 | 18.94 | 12.40 | 13.85 | -5.55 | 4.29 | 31.23 | 24.95 | 18.47 | -4.01 |
Golgi | 0.07 | 14.25 | 15.16 | 9.23 | 2.08 | 3.29 | 26.28 | 19.26 | 17.54 | -8.37 | -0.53 | 29.95 | 24.03 | 20.75 | -4.47 |
Lipid Particles | -0.51 | -4.97 | -7.02 | -6.08 | -4.29 | 8.51 | 5.13 | -2.99 | -7.66 | -6.05 | 6.88 | -0.17 | -7.61 | -10.40 | -7.26 |
Mitochondria | 11.03 | 13.67 | 14.23 | 2.91 | 1.26 | -5.87 | 14.77 | 12.45 | 17.74 | -2.26 | -5.05 | 20.69 | 18.85 | 18.25 | -0.74 |
Mitotic Spindle | -3.44 | -6.21 | -2.21 | -1.61 | 2.63 | 1.80 | -1.81 | -2.16 | -2.99 | -0.71 | -0.26 | -5.75 | -3.28 | -3.22 | 1.09 |
None | -1.02 | 0.66 | -3.18 | 0.93 | -3.36 | 2.33 | 1.29 | 2.95 | 1.73 | 1.14 | -0.71 | 1.02 | 1.23 | 0.91 | -0.19 |
Nuclear Periphery | -1.90 | -6.36 | -4.91 | 1.12 | -0.87 | 1.12 | -1.93 | -2.01 | -2.46 | -0.35 | -1.68 | -4.23 | -4.20 | -1.45 | -0.66 |
Nuclear Periphery Foci | -3.26 | -9.13 | -3.62 | -0.18 | 4.77 | 0.90 | -6.07 | -3.80 | -3.58 | 2.86 | -0.89 | -10.94 | -5.46 | -3.24 | 5.42 |
Nucleolus | -1.23 | 2.17 | 1.86 | 1.51 | -1.45 | -0.07 | 1.37 | 1.39 | 1.62 | 0.85 | -0.83 | 1.98 | 1.93 | 2.04 | -0.70 |
Nucleus | -1.38 | -5.12 | -4.11 | -0.60 | -0.42 | 4.81 | -0.63 | 6.40 | 0.60 | 7.33 | 1.34 | -4.88 | -1.54 | -2.22 | 2.14 |
Peroxisomes | 0.71 | 3.02 | 9.25 | 5.51 | 5.08 | 2.52 | 10.99 | 13.88 | 12.77 | 2.66 | 0.73 | 10.07 | 16.44 | 13.87 | 5.43 |
Vacuole | 7.64 | 12.85 | 14.26 | 3.22 | 2.41 | 2.73 | -6.22 | -1.61 | -3.96 | 4.32 | 13.44 | 6.20 | 11.04 | -1.78 | 5.00 |
Vacuole Periphery | 4.35 | 19.79 | 11.48 | 3.93 | -2.83 | -4.17 | 14.69 | 1.93 | 4.81 | -5.74 | 2.26 | 24.35 | 10.26 | 6.76 | -6.25 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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