Standard name | |
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Human Ortholog | |
Description | Exomer-dependent cargo protein; induces appearance of [PIN+] prion when overproduced; prion-like domain serves as a retention signal in the trans-Golgi network; predicted to be palmitoylated |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 12 | 37 | 108 | 95 | 46 | 58 | 59 | 80 | 43 | 47 | 46 | 57 | 12 | 25 | 24 | 12 | 23 | 17 | 16 | 28 | 93 | 10 | 29 | 17 |
Bud | 3 | 26 | 39 | 39 | 16 | 28 | 45 | 56 | 47 | 48 | 32 | 47 | 4 | 6 | 8 | 1 | 1 | 3 | 9 | 23 | 41 | 11 | 22 | 11 |
Bud Neck | 0 | 0 | 7 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 16 | 54 | 13 | 24 | 10 |
Bud Site | 0 | 3 | 5 | 17 | 0 | 6 | 6 | 0 | 4 | 4 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 6 | 6 | 3 | 3 | 6 | 7 | 6 | 5 | 9 | 5 | 1 | 8 | 5 | 3 | 3 | 4 | 0 | 0 | 3 | 0 | 1 | 0 |
Cytoplasm | 6 | 59 | 98 | 157 | 211 | 283 | 434 | 416 | 301 | 430 | 256 | 345 | 17 | 88 | 105 | 38 | 17 | 31 | 3 | 15 | 17 | 11 | 24 | 31 |
Endoplasmic Reticulum | 9 | 28 | 62 | 19 | 22 | 38 | 20 | 30 | 22 | 31 | 37 | 24 | 16 | 26 | 26 | 7 | 7 | 11 | 13 | 19 | 57 | 7 | 30 | 32 |
Endosome | 0 | 0 | 4 | 14 | 31 | 54 | 75 | 87 | 31 | 64 | 22 | 68 | 1 | 2 | 7 | 37 | 31 | 18 | 4 | 12 | 27 | 6 | 5 | 6 |
Golgi | 0 | 1 | 2 | 1 | 2 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 15 | 32 | 22 | 4 | 4 | 12 | 2 | 0 | 2 |
Mitochondria | 1 | 33 | 0 | 6 | 0 | 2 | 5 | 6 | 36 | 28 | 24 | 47 | 3 | 0 | 2 | 16 | 8 | 7 | 4 | 18 | 26 | 5 | 5 | 7 |
Nucleus | 0 | 1 | 3 | 0 | 0 | 5 | 5 | 4 | 3 | 3 | 5 | 5 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 4 | 1 | 1 | 3 | 4 | 0 | 0 | 1 | 3 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 4 | 10 | 1 | 6 | 14 | 4 | 0 | 5 | 1 | 2 | 0 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 28 | 23 | 12 | 11 | 13 | 20 | 6 | 13 | 5 | 11 | 0 | 3 | 7 | 12 | 10 | 18 | 0 | 0 | 2 | 0 | 0 | 0 |
Vac/Vac Membrane | 3 | 8 | 30 | 15 | 103 | 81 | 120 | 159 | 77 | 104 | 53 | 94 | 0 | 0 | 0 | 62 | 55 | 60 | 25 | 64 | 112 | 20 | 60 | 68 |
Unique Cell Count | 26 | 156 | 311 | 319 | 386 | 488 | 689 | 750 | 490 | 674 | 422 | 584 | 44 | 135 | 165 | 169 | 150 | 150 | 87 | 210 | 465 | 94 | 208 | 196 |
Labelled Cell Count | 34 | 197 | 396 | 404 | 448 | 577 | 806 | 877 | 577 | 785 | 494 | 712 | 54 | 162 | 187 | 207 | 190 | 197 | 87 | 210 | 465 | 94 | 208 | 196 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.9 | 5.7 | 8.3 | 7.7 | 8.4 | 7.5 | 7.7 | 7.9 | 6.6 | 7.4 | 7.3 | 7.3 | 6.8 | 7.4 | 6.4 | 8.4 | 10.4 | 11.6 | 10.6 | 10.8 | 10.6 |
Std Deviation (1e-4) | 1.4 | 0.9 | 1.4 | 1.5 | 1.4 | 1.4 | 1.4 | 1.2 | 1.2 | 1.2 | 1.2 | 1.3 | 1.2 | 3.1 | 1.4 | 1.4 | 1.8 | 2.6 | 8.1 | 8.4 | 7.8 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.03 | -0.14 | -0.11 | -0.08 | -0.32 | -0.16 | -0.19 | -0.18 | -0.3 | -0.17 | -0.37 | 0.02 | 0.33 | 0.48 | 0.35 | 0.39 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.3 | -7.2 | -7.8 | -10.3 | -9.3 | -9.2 | -11.1 | -7.8 | -9.2 | -1.0 | -3.4 | -4.7 | -6.7 | -4.3 | -5.3 |
Bud | -0.1 | -4.1 | -3.4 | -3.2 | -2.6 | -1.3 | -2.8 | -2.2 | -2.2 | -0.7 | -2.6 | -2.7 | -4.5 | -4.2 | -3.7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4.5 | 6.1 | 7.3 | 9.2 | 7.1 | 8.3 | 9.4 | 7.8 | 7.9 | 0.9 | 6.6 | 6.7 | -2.1 | -4.7 | -2.4 |
Endoplasmic Reticulum | -5.2 | -5.7 | -5.1 | -9.1 | -8.4 | -7.0 | -7.7 | -4.4 | -7.6 | 2.5 | -0.2 | -1.1 | -4.7 | -4.3 | -3.5 |
Endosome | 2.3 | 4.1 | 5.2 | 5.2 | 5.5 | 3.4 | 4.7 | 2.8 | 5.4 | 0 | 0 | 0 | 7.7 | 7.4 | 5.1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.7 | 8.0 | 6.4 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4.9 | 3.6 | 4.3 | 5.1 | 0 | 0 | 0 | 5.5 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1.6 | 0 | 0 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -0.8 | -3.3 | -4.3 | -5.3 | -4.5 | -5.3 | -5.2 | -5.0 | -5.0 | 0 | -2.6 | -1.9 | -0.7 | -0.9 | 1.0 |
Vacuole | -2.4 | 5.7 | 2.8 | 3.2 | 4.5 | 2.5 | 2.5 | 1.2 | 2.7 | 0 | -3.7 | -4.1 | 7.2 | 7.0 | 7.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.4634 | 6.1182 | 5.5416 | 5.4354 | 5.0954 | 6.0426 | 5.481 | 6.9288 | 6.011 | 6.2296 | 5.5214 | 6.6209 | 3.3537 | 3.9002 | 3.8501 | 3.7583 | 4.0424 | 4.1797 |
Actin | 0.122 | 0.0117 | 0.0826 | 0.0923 | 0.0464 | 0.0259 | 0.0612 | 0.0655 | 0.0699 | 0.093 | 0.1755 | 0.0374 | 0.0823 | 0.0123 | 0.0381 | 0.0479 | 0.0383 | 0.0238 |
Bud | 0.0289 | 0.0119 | 0.1687 | 0.0604 | 0.0301 | 0.0065 | 0.0154 | 0.0167 | 0.1056 | 0.0693 | 0.0468 | 0.0067 | 0.0448 | 0.0215 | 0.188 | 0.0868 | 0.0155 | 0.0035 |
Bud Neck | 0.0305 | 0.0075 | 0.0094 | 0.0132 | 0.0595 | 0.5829 | 0.0716 | 0.0169 | 0.0033 | 0.0059 | 0.0105 | 0.4514 | 0.0218 | 0.009 | 0.0049 | 0.0065 | 0.1525 | 0.4386 |
Bud Periphery | 0.0901 | 0.013 | 0.3865 | 0.3111 | 0.0962 | 0.0174 | 0.0533 | 0.0132 | 0.2092 | 0.2411 | 0.1526 | 0.0233 | 0.101 | 0.0445 | 0.3594 | 0.2387 | 0.1066 | 0.0096 |
Bud Site | 0.0997 | 0.3028 | 0.0498 | 0.0069 | 0.0172 | 0.0105 | 0.144 | 0.3257 | 0.0459 | 0.0105 | 0.0234 | 0.0098 | 0.0779 | 0.2235 | 0.0367 | 0.0245 | 0.0042 | 0.0042 |
Cell Periphery | 0.0404 | 0.0114 | 0.0059 | 0.0085 | 0.0068 | 0.0089 | 0.026 | 0.0073 | 0.0115 | 0.0094 | 0.018 | 0.0111 | 0.0171 | 0.0171 | 0.0055 | 0.0058 | 0.0115 | 0.0069 |
Cytoplasm | 0.298 | 0.3681 | 0.1177 | 0.247 | 0.1969 | 0.1526 | 0.1831 | 0.2757 | 0.1849 | 0.0997 | 0.2058 | 0.2738 | 0.3513 | 0.3139 | 0.1765 | 0.2049 | 0.316 | 0.2614 |
Cytoplasmic Foci | 0.0425 | 0.0556 | 0.0178 | 0.0131 | 0.0397 | 0.0364 | 0.0496 | 0.062 | 0.0496 | 0.0277 | 0.0535 | 0.0367 | 0.0309 | 0.0458 | 0.0178 | 0.0205 | 0.0592 | 0.0515 |
Eisosomes | 0.0014 | 0.0003 | 0.0002 | 0.0002 | 0.0011 | 0.0001 | 0.0035 | 0.0004 | 0.0005 | 0.0003 | 0.0007 | 0.0003 | 0.0007 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.026 | 0.0076 | 0.0039 | 0.0077 | 0.0108 | 0.0053 | 0.0141 | 0.0122 | 0.0069 | 0.0138 | 0.0071 | 0.0068 | 0.0229 | 0.0117 | 0.0045 | 0.0223 | 0.0052 | 0.015 |
Endosome | 0.0466 | 0.0987 | 0.0529 | 0.098 | 0.1262 | 0.0557 | 0.0954 | 0.1317 | 0.1612 | 0.1758 | 0.203 | 0.0692 | 0.0889 | 0.1213 | 0.0537 | 0.1311 | 0.149 | 0.0885 |
Golgi | 0.0281 | 0.0177 | 0.0203 | 0.0206 | 0.0894 | 0.054 | 0.0411 | 0.0427 | 0.0395 | 0.0498 | 0.042 | 0.0349 | 0.0276 | 0.0265 | 0.0088 | 0.0225 | 0.0253 | 0.0271 |
Lipid Particles | 0.0165 | 0.0059 | 0.0024 | 0.0034 | 0.0604 | 0.0058 | 0.0424 | 0.0026 | 0.0043 | 0.0352 | 0.0164 | 0.002 | 0.0137 | 0.0076 | 0.0017 | 0.0019 | 0.0285 | 0.0051 |
Mitochondria | 0.0115 | 0.0014 | 0.0077 | 0.0046 | 0.0412 | 0.0072 | 0.0588 | 0.0038 | 0.0271 | 0.017 | 0.0154 | 0.0045 | 0.0034 | 0.0027 | 0.0032 | 0.0067 | 0.0071 | 0.0042 |
None | 0.0499 | 0.0341 | 0.0044 | 0.0129 | 0.0572 | 0.0017 | 0.0677 | 0.0012 | 0.0238 | 0.0348 | 0.002 | 0.0043 | 0.0443 | 0.033 | 0.0109 | 0.0014 | 0.036 | 0.0129 |
Nuclear Periphery | 0.0076 | 0.0013 | 0.0015 | 0.0019 | 0.0039 | 0.0068 | 0.0107 | 0.0008 | 0.0019 | 0.0023 | 0.0012 | 0.0006 | 0.0115 | 0.0029 | 0.0018 | 0.0034 | 0.0008 | 0.0047 |
Nucleolus | 0.0009 | 0.0008 | 0.0005 | 0.0002 | 0.0011 | 0.0003 | 0.0016 | 0.0003 | 0.0008 | 0.001 | 0.0002 | 0.0002 | 0.0006 | 0.0014 | 0.0004 | 0.0005 | 0.0003 | 0.0002 |
Nucleus | 0.0027 | 0.0025 | 0.0014 | 0.0013 | 0.0014 | 0.0013 | 0.003 | 0.001 | 0.0017 | 0.0016 | 0.0007 | 0.0009 | 0.0051 | 0.0032 | 0.0024 | 0.0024 | 0.0013 | 0.0009 |
Peroxisomes | 0.0074 | 0.0015 | 0.0033 | 0.0003 | 0.0322 | 0.0018 | 0.0236 | 0.0026 | 0.0027 | 0.0047 | 0.0031 | 0.0013 | 0.0016 | 0.0017 | 0.0008 | 0.0013 | 0.0066 | 0.002 |
Punctate Nuclear | 0.0184 | 0.001 | 0.0009 | 0.0002 | 0.0081 | 0.0034 | 0.0059 | 0.0005 | 0.0009 | 0.0005 | 0.0007 | 0.0003 | 0.0015 | 0.0011 | 0.0013 | 0.0005 | 0.0006 | 0.0005 |
Vacuole | 0.0287 | 0.0419 | 0.0489 | 0.0763 | 0.0641 | 0.0131 | 0.0218 | 0.0152 | 0.0404 | 0.0927 | 0.0173 | 0.022 | 0.0472 | 0.0923 | 0.0725 | 0.1457 | 0.0308 | 0.0362 |
Vacuole Periphery | 0.0022 | 0.0031 | 0.0133 | 0.02 | 0.0101 | 0.0025 | 0.0061 | 0.0019 | 0.0088 | 0.0139 | 0.0041 | 0.0024 | 0.0039 | 0.007 | 0.0111 | 0.0247 | 0.0047 | 0.0031 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.2962 | 9.2945 | 18.1044 | 15.7045 | 11.7954 | 9.0925 | 20.851 | 22.5961 | 17.7959 | 16.0303 |
Translational Efficiency | 0.8347 | 1.935 | 1.0766 | 1.0672 | 1.0459 | 1.6672 | 0.8008 | 0.9265 | 1.2766 | 1.1218 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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