Standard name
Human Ortholog
Description Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.97 0.98 0.95 0.95 0.96 0.93 0.86 0.85 0.82 0.78 0.99 1.0 1.0 – – 0.98 0.86 0.85 0.6 0.9 0.82 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0.05 0.05 0.05 0.27 0.22 0.31 0.28 0 0 0 – – 0 0.05 0.05 0.16 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 – – 0 4 7 5 1 5 3
Bud 0 0 1 1 2 0 1 1 8 5 4 10 0 0 0 – – 0 0 3 3 1 4 3
Bud Neck 1 2 4 4 1 14 20 11 4 4 2 5 1 2 0 – – 0 1 3 2 0 1 1
Bud Site 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 1 5 2 2 0 0 0 1 3 1 1 1 0 2 1 – – 1 1 4 4 1 2 1
Cytoplasm 348 512 239 165 107 275 300 293 291 268 194 195 282 283 294 – – 218 308 430 155 257 241 318
Endoplasmic Reticulum 1 5 1 0 1 0 1 0 0 0 0 0 0 0 0 – – 12 0 2 4 0 1 0
Endosome 1 0 2 0 0 1 0 1 1 0 0 1 0 0 0 – – 2 5 6 7 1 2 3
Golgi 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 – – 0 0 1 3 0 3 0
Mitochondria 7 9 2 1 7 15 15 16 92 68 73 71 1 0 0 – – 6 17 23 41 8 15 9
Nucleus 1 0 0 0 1 1 1 6 1 1 0 4 1 1 0 – – 0 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 1 0 1 0 0 0 0 0 – – 0 0 1 0 1 0 1
Nucleolus 0 0 1 1 0 0 0 3 1 0 0 1 1 0 0 – – 0 0 0 0 0 0 1
Peroxisomes 0 1 1 0 0 0 1 0 0 0 0 1 0 0 0 – – 0 1 1 0 0 0 0
SpindlePole 1 3 1 1 0 4 6 5 2 3 1 4 0 0 0 – – 1 3 7 2 1 2 2
Vac/Vac Membrane 2 1 1 0 1 0 3 1 1 1 0 3 0 0 0 – – 4 7 2 3 2 2 2
Unique Cell Count 355 529 247 168 113 288 312 316 338 316 236 250 284 283 295 222 359 509 258 285 294 361
Labelled Cell Count 363 541 256 176 120 310 348 339 406 352 275 296 286 288 296 244 359 509 258 285 294 361


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.6 5.0 4.4 3.8 4.1 4.1 4.6 3.3 3.7 3.3 3.5 4.4 4.6 4.4 4.0 4.8 4.9 4.7 4.9 5.0
Std Deviation (1e-4) 0.9 0.7 1.9 2.2 1.7 1.7 1.7 2.3 1.6 1.0 1.0 0.9 1.0 1.0 1.5 1.1 1.1 1.2 1.3 1.4 1.2
Intensity Change (Log2) – – – -0.16 -0.38 -0.27 -0.28 -0.11 -0.57 -0.45 -0.6 -0.53 -0.17 -0.12 -0.17 -0.32 -0.05 -0.02 -0.07 -0.01 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1519 2.1495 2.0001 1.6513 2.2487 2.1789 4.9169 4.063 4.4601 5.2725 4.3954 4.6286 1.585 1.6185 1.6766 2.0083 1.4147 1.9379
Actin 0.0384 0.0001 0.016 0.0001 0.0249 0.0035 0.0483 0.0053 0.0217 0.0003 0.0039 0.0008 0.0224 0.0017 0.012 0.0157 0.0001 0.0087
Bud 0.0007 0.0001 0.0041 0 0.0002 0.0008 0.0055 0.0197 0.0078 0.0022 0.01 0.002 0.0023 0.0003 0.0014 0.0001 0.0005 0.0027
Bud Neck 0.0013 0 0.007 0 0.0002 0.0003 0.0009 0.0023 0.002 0.0001 0.0017 0.0031 0.0064 0.0001 0.0007 0.0001 0 0.0007
Bud Periphery 0.0024 0.0002 0.0043 0 0.0004 0.0035 0.0171 0.0235 0.0144 0.0046 0.0545 0.004 0.0083 0.0005 0.0033 0.0001 0.0011 0.0036
Bud Site 0.0037 0.0003 0.0169 0 0.0008 0.0001 0.0043 0.0242 0.0103 0.0001 0.0011 0.0013 0.0062 0.0024 0.0037 0.0004 0 0.0003
Cell Periphery 0.0011 0.0001 0.0003 0 0.0001 0.0002 0.0006 0.0006 0.0013 0.0002 0.0022 0.0002 0.0008 0.0003 0.0002 0 0 0.0001
Cytoplasm 0.0225 0.031 0.0232 0.029 0.0378 0.0367 0.0454 0.0259 0.0499 0.0312 0.0566 0.0516 0.0215 0.0262 0.0182 0.0132 0.028 0.0258
Cytoplasmic Foci 0.0076 0.0027 0.0064 0.0011 0.0166 0.0034 0.0129 0.002 0.0092 0.0075 0.0039 0.0074 0.0154 0.0041 0.0062 0.0089 0.003 0.0018
Eisosomes 0.0008 0.0003 0.0004 0.0001 0.0007 0.0002 0.0004 0.0002 0.0006 0.0001 0.0001 0.0001 0.0006 0.0004 0.0002 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.002 0.0003 0.001 0.0001 0.0011 0.0002 0.0059 0.0006 0.0042 0.0008 0.0024 0.0007 0.0021 0.0003 0.001 0.0002 0.0002 0.0003
Endosome 0.0081 0.0003 0.0036 0 0.0056 0.0003 0.0157 0.0002 0.0067 0.0014 0.0009 0.0007 0.0063 0.0002 0.0011 0.0027 0.0003 0.0005
Golgi 0.0031 0 0.0019 0 0.0036 0.0001 0.0076 0.0001 0.0028 0.0001 0.0002 0.0001 0.004 0.0002 0.001 0.004 0 0.0007
Lipid Particles 0.0144 0.0001 0.0026 0.0001 0.0185 0.0002 0.0037 0.0001 0.0013 0.0001 0.0006 0.0004 0.0047 0.0003 0.0039 0.0076 0.0001 0.0019
Mitochondria 0.0024 0.0002 0.002 0.0001 0.003 0.0005 0.004 0.0006 0.0016 0.0004 0.0011 0.0003 0.0031 0.0002 0.0012 0.0007 0.0004 0.0004
None 0.8674 0.9625 0.8845 0.9687 0.8384 0.9486 0.7886 0.8881 0.8439 0.9463 0.8448 0.9149 0.8629 0.9539 0.9291 0.9144 0.964 0.9459
Nuclear Periphery 0.0051 0.0001 0.0036 0.0001 0.0027 0.0002 0.0174 0.0007 0.0029 0.0013 0.0056 0.0013 0.0095 0.0002 0.0019 0.0007 0.0003 0.0004
Nucleolus 0.0014 0.0002 0.0013 0.0001 0.0007 0.0001 0.0005 0.0006 0.002 0.0002 0.0005 0.0006 0.0009 0.0002 0.0007 0.0001 0.0002 0.0004
Nucleus 0.003 0.0006 0.0058 0.0001 0.0007 0.0003 0.0037 0.0024 0.0047 0.0014 0.0022 0.0041 0.0038 0.0007 0.0024 0.0001 0.0004 0.0016
Peroxisomes 0.0082 0.0001 0.0086 0.0001 0.0298 0.0004 0.009 0.0002 0.0017 0.0001 0.0006 0.0003 0.0121 0.0067 0.0083 0.0274 0.0003 0.0019
Punctate Nuclear 0.0047 0.0004 0.0054 0.0002 0.0135 0.0003 0.003 0.0021 0.0093 0.0005 0.0046 0.0052 0.0028 0.0009 0.0025 0.0033 0.0007 0.0017
Vacuole 0.0013 0.0003 0.0006 0.0001 0.0005 0.0002 0.0029 0.0005 0.0014 0.0009 0.0017 0.0007 0.0026 0.0002 0.0009 0.0001 0.0002 0.0003
Vacuole Periphery 0.0004 0 0.0004 0 0.0003 0 0.0027 0.0001 0.0004 0.0003 0.0007 0.0002 0.0011 0 0.0004 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1538 1463 1767 1580 1749 2176 2823 1402 3287 3639 4590 2982

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 583.14 680.59 751.14 775.34 601.39 774.23 747.72 782.48 592.85 736.58 749.04 778.70
Standard Deviation 85.01 72.78 85.45 108.89 63.10 98.13 91.32 107.44 74.72 99.98 89.13 108.27
Intensity Change Log 2 — 0.222944 0.365240 0.410987 — 0.364461 0.314197 0.379753 — 0.296526 0.339551 0.395214

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000082 0.000564 0.000179 0.000899 0.000060 0.000529 0.000294 0.000999 0.000071 0.000543 0.000249 0.000946
Bud Neck 0.013519 0.044074 0.005637 0.010334 0.016583 0.059229 0.010335 0.011802 0.015149 0.053137 0.008527 0.011024
Bud Site 0.002447 0.018446 0.011990 0.036789 0.002574 0.025494 0.015528 0.036353 0.002515 0.022661 0.014166 0.036584
Cell Periphery 0.000144 0.000155 0.000130 0.000336 0.000108 0.000301 0.000137 0.000268 0.000125 0.000242 0.000134 0.000304
Cytoplasm 0.431776* 0.365945* 0.338741* 0.381929* 0.460855* 0.370840* 0.408831* 0.367681* 0.447249* 0.368872* 0.381849* 0.375230*
Cytoplasmic Foci 0.235555* 0.292947* 0.006586 0.012482 0.215414* 0.214126* 0.006133 0.011644 0.224838* 0.245815* 0.006307 0.012088
Eisosomes 0.000179 0.000160 0.000021 0.000049 0.000152 0.000213 0.000021 0.000044 0.000165 0.000192 0.000021 0.000047
Endoplasmic Reticulum 0.000918 0.001072 0.001173 0.002272 0.000659 0.001015 0.001646 0.003471 0.000780 0.001037 0.001464 0.002836
Endosome 0.015861 0.020836 0.000886 0.007882 0.006461 0.022226 0.000928 0.004280 0.010859 0.021667 0.000912 0.006188
Golgi 0.001918 0.007642 0.000668 0.003028 0.001167 0.006929 0.000078 0.002748 0.001518 0.007216 0.000306 0.002897
Lipid Particles 0.021950 0.008933 0.000515 0.001211 0.009885 0.010499 0.000513 0.001140 0.015530 0.009870 0.000513 0.001177
Mitochondria 0.007407 0.015019 0.000687 0.004290 0.003018 0.019894 0.001215 0.004629 0.005072 0.017934 0.001012 0.004449
Mitotic Spindle 0.000840 0.003540 0.007262 0.072470 0.000592 0.009659 0.008727 0.014362 0.000708 0.007199 0.008163 0.045150
None 0.009007 0.004663 0.005685 0.006422 0.003217 0.005431 0.004851 0.006983 0.005926 0.005122 0.005172 0.006686
Nuclear Periphery 0.000989 0.000397 0.001700 0.002682 0.000544 0.000589 0.001864 0.002112 0.000752 0.000512 0.001800 0.002414
Nuclear Periphery Foci 0.000613 0.000315 0.000142 0.001492 0.000269 0.000285 0.000206 0.001076 0.000430 0.000297 0.000182 0.001297
Nucleolus 0.003582 0.002576 0.000439 0.000900 0.001282 0.002955 0.000496 0.001199 0.002358 0.002803 0.000474 0.001041
Nucleus 0.204985* 0.129598 0.598657* 0.367614* 0.235638* 0.166496* 0.506490* 0.471352* 0.221295* 0.151662* 0.541971* 0.416387*
Peroxisomes 0.008194 0.024694 0.000129 0.000922 0.003714 0.017881 0.000186 0.000932 0.005810 0.020620 0.000164 0.000926
Vacuole 0.038561 0.056130 0.018551 0.082550 0.037168 0.062432 0.031268 0.055976 0.037820 0.059898 0.026373 0.070056
Vacuole Periphery 0.001470 0.002293 0.000221 0.003448 0.000639 0.002977 0.000254 0.000951 0.001028 0.002702 0.000241 0.002274

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.80 -2.45 -5.59 0.12 -4.00 -2.39 -3.06 -3.57 -0.50 -2.31 -3.67 -3.71 -5.81 -0.34 -3.79
Bud Neck -10.93 5.36 3.13 13.41 -3.15 -14.41 4.03 3.59 17.57 -0.27 -18.21 6.02 4.87 22.25 -1.39
Bud Site -9.96 -4.62 -7.98 -0.79 -3.59 -12.14 -7.33 -7.60 0.65 -3.57 -15.48 -8.64 -11.00 0.37 -4.97
Cell Periphery -1.05 1.72 -1.62 -1.19 -2.34 -10.05 -1.82 -7.85 4.20 -6.16 -9.59 0.29 -5.00 2.69 -4.92
Cytoplasm 6.65 12.84 13.60 6.51 0.72 10.72 11.80 16.76 7.15 6.99 12.21 16.73 21.65 9.87 6.43
Cytoplasmic Foci -7.00 42.51 42.02 46.21 -2.89 0.21 42.78 41.43 44.31 -4.27 -4.00 60.28 58.99 63.07 -5.39
Eisosomes 1.57 17.43 12.80 12.25 -15.43 -6.31 22.98 16.31 19.27 -15.20 -3.57 27.52 19.86 22.63 -21.80
Endoplasmic Reticulum -0.84 -4.37 -13.58 -11.93 -11.06 -2.50 -8.02 -14.22 -12.37 -8.11 -2.24 -8.83 -19.80 -17.26 -11.75
Endosome -2.88 12.32 9.32 13.13 -5.32 -13.42 11.23 5.48 16.89 -4.58 -10.50 15.78 10.63 21.20 -7.04
Golgi -6.50 2.92 2.34 7.17 -0.85 -8.54 10.83 -0.26 6.65 -2.38 -10.60 6.39 1.19 9.94 -2.55
Lipid Particles 7.80 14.16 13.60 10.57 -11.86 -0.62 15.19 13.88 13.15 -9.84 6.09 19.06 18.02 16.81 -15.73
Mitochondria -5.25 7.80 5.63 10.54 -3.72 -10.84 6.60 0.26 10.20 -2.50 -11.53 9.48 4.61 14.11 -3.92
Mitotic Spindle -2.42 -4.97 -9.70 -8.72 -6.51 -6.03 -6.11 -5.86 -0.70 -1.49 -6.45 -7.83 -11.20 -7.49 -6.74
None 3.44 3.26 3.68 -0.42 0.11 -3.29 -1.90 -3.61 -0.17 -2.18 1.08 2.10 0.97 -0.26 -1.52
Nuclear Periphery 5.16 -9.08 -11.96 -15.86 -7.53 -0.32 -18.83 -16.47 -13.51 -4.66 2.53 -19.05 -19.24 -19.84 -8.43
Nuclear Periphery Foci 2.28 2.96 -1.82 -5.44 -6.25 -0.22 0.14 -4.90 -4.88 -5.25 1.90 2.71 -4.66 -7.22 -8.04
Nucleolus 1.26 4.75 4.36 5.00 -2.97 -4.81 4.49 1.49 5.68 -1.86 -1.08 5.86 4.66 7.95 -2.83
Nucleus 11.22 -46.38 -23.32 -33.00 19.28 10.87 -41.69 -28.42 -36.97 4.11 14.94 -61.73 -35.87 -48.04 16.17
Peroxisomes -9.48 8.94 8.57 16.50 -2.60 -11.14 13.39 10.71 13.95 -2.57 -14.02 12.68 11.77 21.19 -3.59
Vacuole -6.24 5.08 -23.30 -19.65 -25.13 -8.86 -3.38 -14.81 -9.61 -13.07 -10.86 -0.22 -27.24 -21.09 -26.87
Vacuole Periphery -2.83 5.83 0.75 3.09 -3.36 -8.31 4.16 0.63 8.04 -1.99 -8.00 7.09 0.72 7.42 -3.88
Standard name
Human Ortholog
Description Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Rgs2

Rgs2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rgs2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available