Standard name | |
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Human Ortholog | |
Description | Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 2 | 1 | 4 | 2 | 5 | 6 | 2 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 7 | 3 | 0 | 6 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 16 | 11 | 15 | 2 | 7 | 4 | 4 | 4 | 5 | 2 | 4 | 21 | 15 | 7 | 20 | 15 | 0 | 0 | 1 | 0 | 0 | 1 |
Cytoplasm | 149 | 398 | 281 | 146 | 172 | 163 | 212 | 148 | 177 | 137 | 124 | 132 | 375 | 516 | 489 | 230 | 210 | 211 | 139 | 387 | 282 | 173 | 429 | 421 |
Endoplasmic Reticulum | 3 | 6 | 15 | 3 | 9 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 9 | 17 | 11 | 11 | 9 | 15 | 0 | 3 | 4 | 36 | 49 | 42 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Mitochondria | 0 | 0 | 0 | 0 | 3 | 5 | 18 | 8 | 41 | 73 | 66 | 69 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 2 | 1 | 2 | 3 |
Nucleus | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 3 | 2 | 4 | 3 | 8 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 3 | 1 | 0 | 0 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 3 | 3 | 1 | 7 | 7 | 0 | 5 | 8 | 6 | 0 | 6 | 2 | 2 | 6 | 7 | 6 | 11 | 0 | 5 | 3 | 5 | 9 | 11 |
Unique Cell Count | 151 | 405 | 297 | 150 | 198 | 173 | 232 | 159 | 209 | 193 | 163 | 196 | 384 | 535 | 505 | 243 | 221 | 227 | 147 | 416 | 303 | 226 | 512 | 503 |
Labelled Cell Count | 154 | 412 | 316 | 161 | 210 | 179 | 248 | 172 | 239 | 231 | 201 | 227 | 391 | 558 | 526 | 257 | 247 | 254 | 147 | 416 | 303 | 226 | 512 | 503 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 53.2 | 49.4 | 33.2 | 33.6 | 16.1 | 8.0 | 6.2 | 5.8 | 4.9 | 4.7 | 4.6 | 4.9 | 54.7 | 46.3 | 35.6 | 42.3 | 44.7 | 44.2 | 42.4 | 34.3 | 32.7 |
Std Deviation (1e-4) | 13.8 | 14.2 | 8.4 | 8.3 | 4.6 | 2.7 | 1.7 | 1.2 | 1.4 | 1.2 | 1.1 | 1.5 | 16.3 | 14.1 | 12.2 | 17.1 | 18.1 | 17.4 | 13.9 | 10.9 | 10.7 |
Intensity Change (Log2) | – | – | – | 0.02 | -1.04 | -2.06 | -2.42 | -2.51 | -2.75 | -2.81 | -2.85 | -2.75 | 0.72 | 0.48 | 0.1 | 0.35 | 0.43 | 0.41 | 0.35 | 0.05 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.8 | 1.0 | -2.3 | -1.3 | -1.4 | -2.0 | 0 | 0 | -2.5 | -3.3 | -1.0 | -1.7 | -1.4 | 1.6 | 0.6 |
Cytoplasm | 1.3 | -3.0 | -0.2 | -1.5 | -0.7 | -3.8 | -7.2 | -5.9 | -8.0 | 2.1 | 1.3 | 1.6 | 0 | 0.2 | -0.8 |
Endoplasmic Reticulum | -1.5 | -0.3 | -3.0 | -3.1 | -2.9 | -3.3 | 0 | 0 | -2.8 | -1.9 | -1.3 | -2.2 | -0.3 | -0.5 | 0.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 4.9 | 0 | 8.0 | 0 | 0 | 11.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 54.2569 | 75.1765 | 67.2456 | 53.8748 | 49.4396 | 72.8518 | 54.2584 | 76.1811 | 67.8019 | 63.2645 | 49.2742 | 70.1253 | 49.416 | 76.6626 | 68.6327 | 53.8299 | 72.1491 | 63.9575 |
Actin | 0.0227 | 0.0008 | 0.0005 | 0.0181 | 0.013 | 0.0011 | 0.0147 | 0.0031 | 0.002 | 0.0062 | 0.0024 | 0.0016 | 0.0137 | 0.0015 | 0.0008 | 0.0001 | 0.0099 | 0.0052 |
Bud | 0.0022 | 0.0013 | 0.0014 | 0.0017 | 0.001 | 0.0031 | 0.0023 | 0.0033 | 0.0053 | 0.0008 | 0.0025 | 0.0059 | 0.0019 | 0.0027 | 0.0039 | 0.002 | 0.0005 | 0.0017 |
Bud Neck | 0.0012 | 0.0005 | 0.0008 | 0.0006 | 0.001 | 0.0026 | 0.0043 | 0.0004 | 0.0006 | 0.0005 | 0.0009 | 0.0016 | 0.0013 | 0.0002 | 0.0005 | 0.0004 | 0.0006 | 0.0009 |
Bud Periphery | 0.0038 | 0.0014 | 0.0032 | 0.0015 | 0.0009 | 0.0037 | 0.004 | 0.0013 | 0.0031 | 0.0007 | 0.0028 | 0.0067 | 0.0037 | 0.0017 | 0.0039 | 0.0018 | 0.0023 | 0.0044 |
Bud Site | 0.0037 | 0.0027 | 0.0005 | 0.0007 | 0.0008 | 0.0012 | 0.0036 | 0.0016 | 0.0033 | 0.0003 | 0.0008 | 0.0004 | 0.0051 | 0.0021 | 0.0014 | 0.0001 | 0.0004 | 0.0005 |
Cell Periphery | 0.009 | 0.0114 | 0.0085 | 0.0015 | 0.0021 | 0.0078 | 0.0081 | 0.0065 | 0.0051 | 0.0091 | 0.0045 | 0.0079 | 0.0222 | 0.0203 | 0.0295 | 0.0261 | 0.053 | 0.0272 |
Cytoplasm | 0.6527 | 0.9093 | 0.8248 | 0.7201 | 0.6387 | 0.8043 | 0.7695 | 0.9147 | 0.8793 | 0.7441 | 0.699 | 0.8472 | 0.6535 | 0.8668 | 0.7993 | 0.8418 | 0.7512 | 0.7662 |
Cytoplasmic Foci | 0.0172 | 0.0013 | 0.002 | 0.0119 | 0.0218 | 0.0105 | 0.0115 | 0.0017 | 0.0032 | 0.0065 | 0.0365 | 0.0063 | 0.0165 | 0.0012 | 0.0051 | 0.0016 | 0.0224 | 0.0066 |
Eisosomes | 0.001 | 0 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0006 | 0 | 0 | 0 | 0 | 0.0003 |
Endoplasmic Reticulum | 0.0543 | 0.0157 | 0.0285 | 0.0063 | 0.0061 | 0.0478 | 0.0405 | 0.0196 | 0.0193 | 0.0026 | 0.0198 | 0.0631 | 0.0436 | 0.0065 | 0.0107 | 0.0017 | 0.0023 | 0.0336 |
Endosome | 0.0121 | 0.0033 | 0.0058 | 0.0165 | 0.0197 | 0.0213 | 0.0133 | 0.0019 | 0.0056 | 0.0175 | 0.0409 | 0.0132 | 0.0228 | 0.0015 | 0.0049 | 0.0057 | 0.011 | 0.0132 |
Golgi | 0.0031 | 0.0014 | 0.0004 | 0.0041 | 0.0213 | 0.0039 | 0.0039 | 0.0006 | 0.0013 | 0.0093 | 0.0077 | 0.0065 | 0.0044 | 0.0002 | 0.0011 | 0.0002 | 0.0046 | 0.0044 |
Lipid Particles | 0.0091 | 0.0008 | 0.0012 | 0.0039 | 0.0199 | 0.0014 | 0.0067 | 0.0007 | 0.005 | 0.0086 | 0.0613 | 0.0023 | 0.0086 | 0.0008 | 0.0027 | 0.0001 | 0.0124 | 0.0183 |
Mitochondria | 0.022 | 0.0007 | 0.0087 | 0.049 | 0.0521 | 0.0099 | 0.0081 | 0.0007 | 0.0063 | 0.029 | 0.0244 | 0.0019 | 0.0295 | 0.003 | 0.009 | 0.0016 | 0.0006 | 0.0476 |
None | 0.0059 | 0.0007 | 0.0005 | 0.0049 | 0.0064 | 0.0045 | 0.0044 | 0.007 | 0.0019 | 0.0008 | 0.0175 | 0.0011 | 0.0096 | 0.0003 | 0.0007 | 0.0003 | 0.0007 | 0.0012 |
Nuclear Periphery | 0.0216 | 0.004 | 0.0115 | 0.009 | 0.0061 | 0.0045 | 0.0133 | 0.0032 | 0.0039 | 0.0034 | 0.0095 | 0.0046 | 0.0145 | 0.0015 | 0.0022 | 0.0022 | 0.0015 | 0.0053 |
Nucleolus | 0.0077 | 0.0004 | 0.0009 | 0.0014 | 0.007 | 0.0048 | 0.0053 | 0.0003 | 0.0011 | 0.0002 | 0.0031 | 0.0002 | 0.0059 | 0.0007 | 0.0008 | 0.0003 | 0.0004 | 0.0083 |
Nucleus | 0.0082 | 0.0022 | 0.0048 | 0.0069 | 0.0138 | 0.0072 | 0.0102 | 0.0026 | 0.0028 | 0.0021 | 0.0052 | 0.0022 | 0.008 | 0.0012 | 0.0025 | 0.0026 | 0.0012 | 0.0037 |
Peroxisomes | 0.0051 | 0 | 0.0006 | 0.0017 | 0.0641 | 0.0005 | 0.0039 | 0.0001 | 0.0003 | 0.0113 | 0.0033 | 0.0001 | 0.003 | 0 | 0.0006 | 0 | 0.0164 | 0.0022 |
Punctate Nuclear | 0.0085 | 0.0001 | 0.0001 | 0.0012 | 0.0013 | 0.0003 | 0.0067 | 0.0002 | 0.0002 | 0.0009 | 0.0011 | 0.0006 | 0.0027 | 0 | 0.0003 | 0 | 0.0051 | 0.0009 |
Vacuole | 0.1059 | 0.0373 | 0.0791 | 0.1053 | 0.0812 | 0.0453 | 0.0534 | 0.0281 | 0.0435 | 0.1286 | 0.0418 | 0.0209 | 0.0929 | 0.067 | 0.0981 | 0.0894 | 0.0858 | 0.0352 |
Vacuole Periphery | 0.0232 | 0.0045 | 0.0161 | 0.0332 | 0.0214 | 0.0143 | 0.0118 | 0.0023 | 0.0066 | 0.0174 | 0.0147 | 0.0057 | 0.0359 | 0.0207 | 0.0218 | 0.0218 | 0.0176 | 0.013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 4169.0712 | 4410.0259 | 4628.634 | 3694.311 | 3923.5767 | 3962.9056 | 3875.4257 | 3368.2938 | 3740.0597 | 3181.6917 |
Translational Efficiency | 0.4671 | 0.486 | 0.5008 | 0.5834 | 0.5072 | 0.435 | 0.5001 | 0.5499 | 0.4593 | 0.5035 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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