Standard name
Human Ortholog
Description Glutamine tRNA synthetase; monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Gln); N-terminal domain proposed to be involved in enzyme-tRNA interactions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.08 0.06 0.07 0.1 – 0.08 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.99 0.94 0.9 0.83 0.79 0.82 – 0.78 0.76 1.0 0.98 0.98 0.98 0.99 0.99 0.95 0.97 0.97 0.81 0.79 0.85
Endoplasmic Reticulum 0 0 0 0 0.06 0.08 0.12 0.14 0.1 – 0.08 0.16 0 0 0 0 0 0.06 0 0 0 0.11 0.17 0.09
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 – 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 1 9 14 16 14 – 6 18 0 0 0 0 0 0 0 0 0 0 0 2
Bud 0 0 0 0 0 0 1 0 1 – 1 0 0 0 0 1 0 0 1 2 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 12 4 8 24 29 34 35 – 17 38 1 1 3 7 6 12 0 0 0 0 0 0
Cytoplasm 101 336 283 159 253 286 389 376 281 – 163 223 81 181 289 373 350 328 105 331 286 75 200 219
Endoplasmic Reticulum 0 0 12 0 17 24 55 67 33 – 16 46 0 3 5 15 10 21 1 1 3 10 42 24
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 1 0 1 1 0
Golgi 0 0 0 0 0 1 1 2 1 – 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1 0 1 0 2 8 4 16 – 20 11 0 0 0 1 0 0 0 0 0 1 0 0
Nucleus 0 0 2 0 2 0 3 5 1 – 1 0 0 0 1 0 0 3 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 0 1 1 0 6 7 2 – 8 4 0 1 0 5 4 3 0 3 1 1 4 7
Unique Cell Count 102 338 293 161 269 318 468 478 342 209 295 81 185 294 381 355 332 112 343 294 94 254 259
Labelled Cell Count 103 342 309 165 282 346 506 511 384 233 342 82 186 299 403 370 367 112 343 294 94 254 259


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 40.9 41.9 29.2 26.4 24.4 19.5 18.4 18.2 16.2 – 13.6 15.2 39.4 38.6 36.8 53.0 54.3 52.8 34.4 36.2 39.3
Std Deviation (1e-4) 8.4 9.3 5.5 6.2 5.4 4.2 3.9 3.5 3.6 – 3.4 3.3 7.4 9.6 8.8 15.5 13.1 14.4 9.0 8.7 10.4
Intensity Change (Log2) – – – -0.15 -0.26 -0.58 -0.67 -0.69 -0.85 – -1.1 -0.94 0.43 0.4 0.33 0.86 0.89 0.85 0.24 0.31 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 3.0 3.2 3.5 – 0 4.3 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery -0.9 -0.7 1.8 1.2 1.7 2.9 – 1.9 3.8 0 -2.3 -2.4 -1.8 -1.9 -0.3
Cytoplasm 1.4 -1.4 -3.2 -5.6 -6.9 -5.7 – -6.5 -7.3 1.7 0.8 1.3 1.0 1.7 1.9
Endoplasmic Reticulum 0 1.2 1.8 3.6 4.4 2.7 – 1.7 4.7 0 -1.5 -1.7 -0.1 -0.9 1.2
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.7 – 5.4 3.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 26.6756 35.2479 30.9508 27.2502 25.0742 35.2975 33.166 45.0687 39.5356 37.2121 33.9701 40.4409 29.9341 40.9059 35.5605 31.4241 33.751 36.8951
Actin 0.0153 0.0003 0.0074 0.0264 0.0297 0.0011 0.0037 0.0002 0.0023 0.0016 0.0062 0.0008 0.0079 0.0004 0.0181 0.0001 0.0023 0.0007
Bud 0.0011 0.0003 0.0015 0.001 0.0008 0.001 0.0009 0.0002 0.0007 0.001 0.0008 0.0004 0.0008 0.0002 0.0007 0.0007 0.0005 0.0003
Bud Neck 0.0027 0.0004 0.0013 0.0006 0.0009 0.0019 0.0007 0.0003 0.0007 0.0009 0.0008 0.0013 0.0007 0.0002 0.0005 0.0009 0.0004 0.0011
Bud Periphery 0.0012 0.0002 0.0026 0.0006 0.001 0.0011 0.0015 0.0001 0.0007 0.0014 0.0005 0.0004 0.0006 0.0001 0.0009 0.0004 0.0004 0.0002
Bud Site 0.0045 0.0006 0.0025 0.0011 0.0046 0.0001 0.0018 0.0002 0.0006 0.0026 0.0127 0.0001 0.0008 0.0002 0.001 0.0002 0.0001 0
Cell Periphery 0.0018 0.0016 0.0018 0.0004 0.0003 0.0008 0.0037 0.0013 0.0014 0.0009 0.0003 0.0006 0.0011 0.0005 0.0004 0.0014 0.0002 0.0003
Cytoplasm 0.741 0.9617 0.827 0.7655 0.7259 0.8813 0.8267 0.9577 0.9001 0.8613 0.848 0.8941 0.7895 0.9761 0.9079 0.8473 0.8615 0.9003
Cytoplasmic Foci 0.0102 0.0015 0.0093 0.0153 0.022 0.0045 0.0121 0.0012 0.0062 0.0078 0.0156 0.0068 0.0133 0.0014 0.0062 0.0026 0.0067 0.0042
Eisosomes 0.0006 0 0.0002 0.0006 0.0004 0 0.0001 0 0.0001 0.0001 0 0 0.0001 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0781 0.0115 0.022 0.012 0.0086 0.0675 0.0416 0.0274 0.015 0.0174 0.0106 0.0542 0.07 0.0116 0.0163 0.02 0.0155 0.0502
Endosome 0.0153 0.0023 0.0104 0.0171 0.0149 0.0127 0.0194 0.0022 0.0119 0.0225 0.0291 0.0131 0.0263 0.0018 0.0103 0.0139 0.0306 0.013
Golgi 0.0028 0.0002 0.0011 0.0067 0.0039 0.0011 0.0061 0.0004 0.0022 0.0028 0.0122 0.0029 0.0035 0.0003 0.0033 0.0002 0.005 0.0024
Lipid Particles 0.0073 0.0001 0.0056 0.02 0.0103 0.0001 0.0052 0.0002 0.0024 0.0051 0.018 0.001 0.0037 0.0001 0.0045 0.0003 0.0014 0.0001
Mitochondria 0.0058 0.0001 0.0104 0.0187 0.0651 0.0011 0.011 0.0003 0.0091 0.0099 0.0058 0.0107 0.0069 0.0002 0.0044 0.0005 0.0333 0.015
None 0.0178 0.0001 0.0009 0.0032 0.0455 0.0003 0.0016 0.0001 0.0007 0.0021 0.0008 0.0004 0.0047 0.0001 0.0019 0.0005 0.0009 0.0003
Nuclear Periphery 0.0177 0.0021 0.0067 0.0136 0.0055 0.0069 0.0105 0.001 0.0027 0.0026 0.0037 0.0017 0.0143 0.0008 0.0031 0.0054 0.0114 0.0019
Nucleolus 0.0016 0 0.0005 0.0007 0.0034 0 0.0012 0 0.0002 0.0003 0.0002 0.0001 0.0012 0 0.0003 0.0001 0.0001 0
Nucleus 0.007 0.0013 0.0035 0.0045 0.0049 0.0019 0.0071 0.0005 0.0012 0.0029 0.0014 0.0009 0.0069 0.0007 0.0014 0.0033 0.0032 0.0009
Peroxisomes 0.0077 0 0.0051 0.0062 0.0152 0 0.0019 0 0.0017 0.0014 0.006 0.0001 0.0063 0 0.0033 0 0.0002 0.0001
Punctate Nuclear 0.005 0 0.0016 0.0012 0.0237 0.0001 0.0028 0 0.0003 0.0014 0.0031 0.0002 0.0061 0 0.0006 0.0001 0.0012 0
Vacuole 0.0457 0.0139 0.0662 0.0592 0.0107 0.0135 0.0347 0.0062 0.033 0.0455 0.0182 0.0084 0.0298 0.0046 0.0123 0.0899 0.0168 0.007
Vacuole Periphery 0.0099 0.0016 0.0125 0.0254 0.0029 0.0032 0.0058 0.0005 0.0068 0.0084 0.0059 0.0016 0.0056 0.0004 0.0023 0.0124 0.0084 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 182.3115 172.9203 177.4111 200.3632 189.5708 142.5009 149.5986 153.8773 150.2345 175.4805
Translational Efficiency 1.6014 1.8489 1.7031 1.4568 1.4848 1.8946 1.5696 1.4447 1.4851 1.5266

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1453 1439 1276 2722 1816 2182 2021 316 3269 3621 3297 3038

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2401.33 2860.05 4219.15 3294.41 2304.12 2765.67 3949.91 3408.52 2347.33 2803.18 4054.11 3306.28
Standard Deviation 392.63 576.78 769.11 586.00 472.40 561.35 611.70 692.79 441.39 569.41 689.56 599.01
Intensity Change Log 2 — 0.252207 0.813119 0.456186 — 0.263413 0.777604 0.564930 — 0.257705 0.795837 0.510459

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000054 0.000131 0.000167 0.000072 0.000034 0.000059 0.000148 0.000152 0.000043 0.000088 0.000155 0.000080
Bud Neck 0.000194 0.000538 0.002561 0.000387 0.000125 0.000170 0.001270 0.002331 0.000156 0.000316 0.001769 0.000590
Bud Site 0.002938 0.003774 0.004143 0.002930 0.001954 0.002661 0.003962 0.004792 0.002392 0.003103 0.004032 0.003124
Cell Periphery 0.001166 0.004235 0.019406 0.007162 0.001712 0.000954 0.004366 0.021489 0.001470 0.002258 0.010187 0.008653
Cytoplasm 0.913712* 0.885290* 0.779451* 0.893524* 0.941134* 0.943558* 0.836902* 0.678329* 0.928946* 0.920402* 0.814667* 0.871141*
Cytoplasmic Foci 0.010268 0.009064 0.006678 0.001983 0.005219 0.006501 0.006205 0.010439 0.007463 0.007519 0.006388 0.002862
Eisosomes 0.000000 0.000002 0.000004 0.000001 0.000000 0.000000 0.000002 0.000003 0.000000 0.000001 0.000003 0.000001
Endoplasmic Reticulum 0.001293 0.001224 0.000472 0.000377 0.000339 0.000324 0.000315 0.000772 0.000763 0.000681 0.000376 0.000418
Endosome 0.000931 0.001409 0.000522 0.000292 0.000417 0.000664 0.000668 0.001353 0.000646 0.000960 0.000611 0.000402
Golgi 0.022629 0.036027 0.020352 0.006334 0.014057 0.012762 0.014437 0.043098 0.017867 0.022007 0.016726 0.010158
Lipid Particles 0.002323 0.007952 0.014670 0.004826 0.002586 0.002169 0.010056 0.022862 0.002469 0.004468 0.011842 0.006702
Mitochondria 0.000069 0.000065 0.000145 0.000175 0.000064 0.000084 0.000127 0.000055 0.000066 0.000076 0.000134 0.000163
Mitotic Spindle 0.000699 0.001410 0.001425 0.000294 0.000354 0.000625 0.001310 0.002421 0.000507 0.000937 0.001354 0.000515
None 0.000939 0.001558 0.001822 0.000662 0.001535 0.001663 0.001281 0.000971 0.001270 0.001621 0.001490 0.000694
Nuclear Periphery 0.000056 0.000156 0.001194 0.000091 0.000045 0.000038 0.000332 0.000513 0.000050 0.000085 0.000666 0.000135
Nuclear Periphery Foci 0.004661 0.010011 0.012823 0.002046 0.003419 0.002767 0.007684 0.016618 0.003971 0.005646 0.009673 0.003561
Nucleolus 0.000022 0.000028 0.000066 0.000078 0.000035 0.000035 0.000060 0.000090 0.000029 0.000032 0.000062 0.000079
Nucleus 0.003151 0.005070 0.024815 0.004724 0.004224 0.003934 0.012489 0.013762 0.003747 0.004385 0.017260 0.005664
Peroxisomes 0.000018 0.000018 0.000013 0.000054 0.000031 0.000029 0.000022 0.000026 0.000025 0.000024 0.000019 0.000051
Vacuole 0.016725 0.019188 0.087511 0.064017 0.011168 0.013381 0.084096 0.149793 0.013638 0.015688 0.085418 0.072939
Vacuole Periphery 0.018149 0.012851 0.021760 0.009972 0.011547 0.007623 0.014268 0.030132 0.014481 0.009701 0.017167 0.012069

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.97 -7.16 -2.33 2.47 5.56 -3.14 -5.74 -6.99 -5.33 0.71 -4.90 -8.23 -4.93 -0.05 5.25
Bud Neck -4.55 -8.25 -3.71 0.99 7.48 -1.82 -10.10 -4.63 -4.56 -2.05 -4.74 -12.39 -6.33 -4.29 8.23
Bud Site -1.49 -2.19 -0.25 1.49 2.21 -2.02 -4.96 -4.40 -3.29 -0.64 -2.28 -4.96 -2.71 -0.44 2.85
Cell Periphery -6.99 -16.16 -11.29 -4.28 10.23 2.70 -6.99 -7.42 -7.76 -6.21 -3.40 -17.15 -12.68 -11.01 2.99
Cytoplasm 4.09 16.57 4.01 -0.92 -14.71 -0.46 19.02 16.18 16.35 8.58 2.35 24.66 13.10 10.93 -11.84
Cytoplasmic Foci 1.11 2.34 10.22 11.79 9.26 -2.10 -3.28 -3.99 -3.08 -2.47 -0.23 -0.22 9.24 11.35 10.79
Eisosomes -2.94 -6.14 -5.14 1.27 5.28 -2.21 -6.57 -6.34 -6.04 -1.90 -3.33 -8.63 -7.84 -2.06 5.70
Endoplasmic Reticulum 0.16 2.42 2.73 4.11 0.74 0.22 0.35 -1.81 -1.84 -1.90 0.47 2.52 2.08 2.32 -0.32
Endosome -2.78 3.45 6.48 7.05 4.64 -2.12 -4.57 -6.70 -4.15 -4.32 -3.18 -0.66 3.72 5.56 4.71
Golgi -4.99 0.96 9.48 13.47 13.14 0.89 -0.65 -6.78 -7.20 -6.71 -3.02 0.61 7.06 10.27 8.75
Lipid Particles -6.92 -12.89 -4.76 3.62 10.40 0.86 -9.62 -8.31 -8.51 -4.52 -4.84 -15.52 -8.41 -4.18 8.15
Mitochondria 0.19 -4.31 -3.35 -3.43 -0.59 -1.69 -2.10 0.64 2.47 2.28 -1.09 -3.40 -3.52 -3.16 -0.53
Mitotic Spindle -2.40 -5.02 2.10 3.45 6.22 -2.21 -6.32 -2.52 -2.19 -1.10 -3.07 -7.83 -0.30 1.91 5.19
None -2.27 -6.33 2.22 3.35 9.39 -0.47 0.71 3.31 2.85 1.06 -1.77 -1.02 4.92 4.93 3.85
Nuclear Periphery -6.48 -12.54 -2.71 2.84 12.05 0.93 -11.17 -8.07 -8.16 -1.93 -4.90 -15.81 -6.14 -3.48 13.18
Nuclear Periphery Foci -6.04 -9.38 5.60 9.66 12.77 1.56 -8.62 -7.73 -8.19 -4.44 -3.95 -12.53 1.04 4.91 13.46
Nucleolus -1.23 -15.49 -3.74 -2.88 -0.50 0.02 -2.50 -4.40 -4.93 -3.22 -0.44 -5.45 -3.48 -3.24 -0.97
Nucleus -2.52 -16.12 -4.50 -0.20 14.72 0.47 -11.33 -5.92 -6.20 -0.48 -1.47 -19.38 -5.54 -4.34 15.83
Peroxisomes 0.06 1.62 -6.15 -6.06 -6.99 0.23 0.56 0.09 -0.19 -0.66 0.15 0.70 -3.30 -4.67 -5.19
Vacuole -1.21 -16.28 -16.54 -15.87 6.06 -1.49 -21.27 -13.59 -13.32 -5.88 -1.68 -26.57 -22.92 -21.72 5.01
Vacuole Periphery 2.56 -1.67 4.63 2.11 7.67 3.09 -1.80 -4.66 -5.89 -3.98 4.16 -2.08 2.33 -2.30 5.09
Standard name
Human Ortholog
Description Glutamine tRNA synthetase; monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Gln); N-terminal domain proposed to be involved in enzyme-tRNA interactions
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Gln4

Gln4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gln4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available