Standard name
Human Ortholog
Description m(7)GpppX pyrophosphatase regulator; non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance; DCS2 has a paralog, DCS1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 – 0 0 0 0 0 0
Cytoplasm 0.84 0.97 0.97 0.95 0.96 0.87 0.78 0.73 0.62 0.67 0.54 0.64 0.99 1.0 0.98 0.95 0.9 – 0.81 0.9 0.81 0.95 0.95 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.25 0 0 0.06 0.05 0.25 0.37 0.3 0.41 0.39 0.49 0.35 0 0 0 0.05 0 – 0.1 0 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.1 0.08 0.1 0.14 0.13 0 0 0 0 0.05 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 – 1 1 2 0 0 0
Bud 0 2 1 3 1 4 19 10 10 11 9 12 0 0 0 1 0 – 0 0 1 0 2 2
Bud Neck 0 0 1 0 1 1 2 1 0 1 0 0 0 0 0 0 1 – 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 2 2 3 3 3 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 1 1 1 1 0 5 1 2 3 4 0 0 3 2 2 – 0 0 1 0 0 0
Cytoplasm 112 131 193 264 365 317 481 457 160 264 129 233 228 300 353 35 37 – 119 133 161 40 231 231
Endoplasmic Reticulum 1 0 1 2 0 0 1 1 0 1 1 2 0 1 3 1 0 – 1 0 2 0 0 0
Endosome 0 0 1 2 1 6 1 2 3 1 0 0 2 0 2 1 1 – 2 0 2 0 1 1
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0
Mitochondria 33 3 1 16 18 91 229 187 105 154 118 128 0 0 0 2 0 – 14 3 16 0 0 2
Nucleus 0 2 0 0 1 4 14 15 14 21 7 12 0 1 1 0 0 – 0 0 0 0 1 1
Nuclear Periphery 1 1 0 0 0 0 6 15 6 3 7 11 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 1 1 2 3 6 6 3 4 1 3 0 0 0 0 1 – 1 0 2 0 1 2
Vac/Vac Membrane 0 1 1 3 4 8 25 63 21 40 33 47 0 1 1 1 2 – 3 2 1 0 1 2
Unique Cell Count 133 135 199 277 379 364 620 624 259 395 239 364 230 300 359 37 41 148 148 199 42 243 251
Labelled Cell Count 147 140 202 294 394 435 784 764 325 506 311 457 230 303 363 43 45 148 148 199 42 243 251


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 3.9 4.3 4.1 4.2 3.7 3.7 4.1 4.6 5.0 5.5 5.9 5.5 6.2 5.4 6.3 7.1 – 5.2 6.0 6.3
Std Deviation (1e-4) 0.4 0.9 1.4 2.1 2.2 2.1 1.8 2.2 3.0 3.1 3.7 3.9 1.2 9.0 1.3 1.5 2.9 – 0.5 1.5 1.6
Intensity Change (Log2) – – – -0.07 -0.03 -0.22 -0.2 -0.05 0.11 0.21 0.36 0.47 0.35 0.53 0.34 0.55 0.72 – 0.27 0.48 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6131 0.7309 1.0526 0.9385 2.0845 1.2004 -0.9127 -0.2837 -0.261 -0.5618 -0.6874 -0.3503 -1.7904 -1.3169 -1.7519 -1.1548 -1.7202 -1.1626
Actin 0.0563 0.001 0.0303 0.0003 0.0493 0.0131 0.0436 0.0002 0.024 0.0037 0.0002 0.0096 0.017 0.0003 0.0052 0.0027 0.0014 0.0102
Bud 0.0081 0.0021 0.0018 0.009 0.0078 0.0019 0.0011 0.0006 0.002 0.0008 0.0001 0.0003 0.0004 0.0039 0.0026 0.0115 0.0001 0.0066
Bud Neck 0.0136 0.0004 0.0097 0.0003 0.0042 0.0141 0.0013 0.0001 0.0008 0.0002 0.0001 0.001 0.0008 0.0001 0.0018 0.0037 0.0001 0.0009
Bud Periphery 0.0157 0.0054 0.0028 0.0129 0.0193 0.0034 0.0014 0.0019 0.0014 0.0005 0 0.0004 0.0004 0.0084 0.0022 0.013 0.0001 0.0138
Bud Site 0.0175 0.0077 0.0112 0.0035 0.0082 0.0013 0.0018 0.0011 0.0125 0.0002 0 0.0006 0.0056 0.0051 0.0101 0.0159 0.0001 0.0021
Cell Periphery 0.0022 0.0002 0.0005 0.0005 0.0005 0.0002 0.0003 0.0002 0.0003 0.0001 0 0.0001 0.0002 0.0002 0.0001 0.0002 0 0.0002
Cytoplasm 0.1442 0.1563 0.1437 0.3081 0.1232 0.2439 0.3928 0.5775 0.5414 0.6351 0.6779 0.6751 0.1718 0.3027 0.233 0.3578 0.2764 0.4118
Cytoplasmic Foci 0.0272 0.0038 0.0131 0.0072 0.0498 0.0164 0.0447 0.0057 0.0397 0.006 0.0039 0.0347 0.0202 0.007 0.0108 0.0299 0.0241 0.0146
Eisosomes 0.0006 0.0002 0.0004 0.0001 0.0013 0.0004 0.0002 0 0.0001 0 0 0.0001 0.0008 0.0001 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0089 0.0005 0.0026 0.0013 0.0044 0.0027 0.0055 0.0027 0.0043 0.006 0.0019 0.0022 0.0017 0.0037 0.0012 0.0018 0.0008 0.0049
Endosome 0.0292 0.0004 0.0072 0.0015 0.0217 0.0121 0.0306 0.0032 0.0344 0.006 0.0012 0.0124 0.0147 0.0022 0.0033 0.0106 0.0072 0.018
Golgi 0.0108 0.0001 0.0049 0.0002 0.0064 0.0036 0.0077 0.0002 0.0116 0.0005 0.0001 0.0113 0.0082 0.0001 0.0027 0.008 0.01 0.0114
Lipid Particles 0.007 0.0001 0.0034 0.0004 0.0135 0.0045 0.0283 0.0002 0.0059 0.0007 0 0.0102 0.0168 0.0023 0.0025 0.0028 0.0021 0.0059
Mitochondria 0.0079 0.0007 0.01 0.0014 0.0087 0.01 0.0037 0.0002 0.0096 0.0007 0.0001 0.004 0.0083 0.0002 0.001 0.0014 0.0027 0.0099
None 0.6023 0.8172 0.736 0.6425 0.5333 0.6452 0.4004 0.4012 0.2882 0.3325 0.3123 0.2293 0.7069 0.659 0.7169 0.5306 0.6165 0.4729
Nuclear Periphery 0.0126 0.0002 0.0082 0.0007 0.0268 0.0057 0.0054 0.0008 0.0027 0.0013 0.0004 0.0015 0.0014 0.0011 0.0004 0.001 0.0003 0.0077
Nucleolus 0.0014 0.0002 0.001 0.0008 0.0039 0.0006 0.002 0.0001 0.0007 0 0 0.0001 0.0009 0.0002 0.0002 0.0003 0 0.0004
Nucleus 0.0151 0.001 0.0039 0.0025 0.0298 0.0042 0.0018 0.001 0.0021 0.0012 0.001 0.001 0.0012 0.0009 0.0014 0.0019 0.0003 0.0013
Peroxisomes 0.0065 0.0012 0.0031 0.0004 0.0606 0.0035 0.0184 0.0001 0.0092 0.0001 0 0.0024 0.0122 0.0002 0.0016 0.0029 0.0561 0.0019
Punctate Nuclear 0.0066 0.0006 0.0045 0.0009 0.0226 0.0072 0.0053 0.0005 0.0023 0.0003 0.0002 0.002 0.0073 0.001 0.0021 0.0011 0.0003 0.0027
Vacuole 0.0046 0.0005 0.001 0.0049 0.0026 0.0041 0.0028 0.0023 0.0054 0.0036 0.0005 0.001 0.0025 0.0012 0.0006 0.0022 0.0011 0.0021
Vacuole Periphery 0.0018 0 0.0006 0.0006 0.0022 0.0017 0.0009 0.0002 0.0014 0.0005 0.0001 0.0005 0.0005 0.0001 0.0001 0.0004 0.0002 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.5975 4.644 6.2028 5.6473 3.3677 9.3457 6.1041 7.9229 9.4293 6.0762
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
541 336 1881 1304 961 456 1953 597 1502 792 3834 1901

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 650.01 696.98 681.22 755.54 681.17 645.12 713.01 795.11 669.95 667.12 697.41 767.97
Standard Deviation 66.43 80.93 112.37 201.40 87.00 227.26 108.74 122.85 81.58 182.13 111.68 181.39
Intensity Change Log 2 — 0.100655 0.067659 0.217046 — -0.078447 0.065907 0.223140 — 0.011787 0.066763 0.220167

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000085 0.000333 0.000272 0.000470 0.000058 0.000206 0.000104 0.002806 0.000068 0.000260 0.000186 0.001203
Bud Neck 0.018022 0.026774 0.007474 0.009462 0.002172 0.027712 0.005644 0.007892 0.007881 0.027314 0.006542 0.008969
Bud Site 0.002550 0.016021 0.017405 0.029483 0.001287 0.008726 0.010234 0.053260 0.001742 0.011821 0.013752 0.036950
Cell Periphery 0.000144 0.000130 0.000200 0.000228 0.000154 0.000167 0.000103 0.000201 0.000150 0.000151 0.000151 0.000220
Cytoplasm 0.414525* 0.370185* 0.413666* 0.403540* 0.645854* 0.425149* 0.521771* 0.351100* 0.562532* 0.401831* 0.468734* 0.387071*
Cytoplasmic Foci 0.271068* 0.222284* 0.013527 0.031352 0.102608 0.262785* 0.016833 0.018466 0.163285 0.245603* 0.015211 0.027305
Eisosomes 0.000172 0.000113 0.000032 0.000072 0.000054 0.000157 0.000028 0.000046 0.000096 0.000139 0.000030 0.000064
Endoplasmic Reticulum 0.000389 0.000690 0.001765 0.002102 0.000579 0.000472 0.000773 0.002057 0.000511 0.000565 0.001260 0.002088
Endosome 0.013019 0.030493 0.002612 0.008991 0.003781 0.021039 0.001796 0.005569 0.007109 0.025050 0.002196 0.007916
Golgi 0.001401 0.002577 0.000926 0.002064 0.000282 0.004368 0.000447 0.004353 0.000685 0.003608 0.000682 0.002783
Lipid Particles 0.008568 0.006124 0.001130 0.002341 0.002306 0.008347 0.000735 0.001131 0.004562 0.007404 0.000929 0.001961
Mitochondria 0.004761 0.007575 0.002079 0.010488 0.000361 0.005583 0.000738 0.007744 0.001946 0.006428 0.001396 0.009626
Mitotic Spindle 0.000393 0.012415 0.011331 0.030636 0.001097 0.004376 0.009724 0.036291 0.000844 0.007787 0.010513 0.032412
None 0.001087 0.002801 0.006866 0.004301 0.006722 0.003197 0.004188 0.014436 0.004692 0.003029 0.005502 0.007484
Nuclear Periphery 0.000555 0.001103 0.001545 0.001852 0.000328 0.000374 0.000920 0.001083 0.000410 0.000683 0.001226 0.001611
Nuclear Periphery Foci 0.000189 0.000556 0.000548 0.000962 0.000790 0.000276 0.000402 0.001076 0.000573 0.000395 0.000474 0.000998
Nucleolus 0.000879 0.003167 0.000789 0.001015 0.000676 0.001502 0.000336 0.000404 0.000749 0.002208 0.000558 0.000823
Nucleus 0.200654* 0.172121* 0.475821* 0.325436* 0.205635* 0.144260* 0.379574* 0.393745* 0.203841* 0.156080* 0.426794* 0.346888*
Peroxisomes 0.008247 0.026417 0.001275 0.002435 0.000527 0.015904 0.000210 0.002452 0.003308 0.020364 0.000732 0.002441
Vacuole 0.052607 0.096239 0.038708 0.127461 0.024429 0.063770 0.045072 0.088338 0.034578 0.077545 0.041950 0.115174
Vacuole Periphery 0.000685 0.001881 0.002030 0.005308 0.000299 0.001631 0.000369 0.007554 0.000438 0.001737 0.001184 0.006013

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.83 -3.94 -4.35 -1.24 -1.79 -3.28 -3.26 -2.30 -1.98 -2.18 -5.09 -4.93 -3.86 -2.58 -2.98
Bud Neck -1.89 3.89 3.41 4.70 -0.81 -6.76 -4.18 -4.52 5.72 0.04 -6.80 1.10 0.11 7.02 -1.19
Bud Site -3.88 -6.49 -7.01 -0.82 -1.26 -4.00 -5.66 -5.21 -3.17 -3.32 -5.56 -8.80 -9.03 -3.21 -3.61
Cell Periphery 0.61 -1.66 -1.01 -1.50 0.80 -0.08 2.59 1.01 0.96 -2.98 0.21 0.88 0.02 -0.23 -1.19
Cytoplasm 2.63 2.22 5.60 1.50 4.49 14.72 17.20 28.39 8.41 16.13 13.62 15.70 26.48 6.59 14.51
Cytoplasmic Foci 3.66 27.78 26.69 17.85 -5.45 -14.59 20.82 21.33 24.63 2.85 -9.06 31.31 30.04 29.84 -3.52
Eisosomes 3.94 11.41 8.43 5.77 -11.07 -9.59 7.78 -1.18 9.18 -9.34 -5.15 12.82 5.81 10.33 -14.76
Endoplasmic Reticulum -3.31 -14.72 -17.12 -12.14 -4.13 1.16 -6.24 -11.46 -11.94 -9.07 -1.17 -14.36 -19.56 -18.21 -10.13
Endosome -3.78 5.89 3.99 5.88 -3.74 -7.82 4.05 1.48 8.32 -2.17 -7.76 6.91 2.93 9.16 -4.57
Golgi -1.41 1.12 0.64 2.03 -0.45 -2.66 -0.14 -1.57 1.74 -1.45 -3.08 0.62 -1.00 2.45 -1.34
Lipid Particles 1.91 7.72 6.63 4.14 -4.71 -6.21 8.32 5.36 7.41 -4.71 -3.66 9.66 6.93 8.01 -6.37
Mitochondria -0.79 1.40 -0.19 0.75 -2.47 -5.60 -3.16 -2.03 1.76 -1.74 -3.41 0.89 -2.04 1.20 -3.20
Mitotic Spindle -2.44 -6.06 -8.06 -2.64 -4.48 -1.33 -5.41 -5.25 -3.93 -2.92 -2.73 -7.89 -9.44 -5.13 -5.44
None -1.49 -9.67 -4.96 -1.14 2.88 2.31 2.15 -1.06 -2.99 -2.87 1.68 -0.37 -0.82 -2.75 -0.76
Nuclear Periphery -1.14 -13.28 -16.18 -4.83 -8.55 -1.04 -19.43 -15.70 -15.36 -6.89 -1.34 -22.80 -21.52 -10.25 -11.42
Nuclear Periphery Foci -2.25 -7.07 -8.62 -2.20 -5.06 4.00 1.91 -2.17 -7.83 -5.00 0.98 0.28 -5.31 -5.84 -6.75
Nucleolus -3.44 1.18 -0.03 3.40 -0.96 -3.00 3.31 3.05 4.65 -0.80 -4.45 2.69 0.32 4.50 -1.85
Nucleus 1.87 -26.41 -12.58 -12.21 14.28 5.78 -22.03 -15.98 -18.59 -3.06 5.47 -34.84 -20.23 -22.24 8.44
Peroxisomes -3.69 4.44 4.32 5.53 -0.39 -6.99 3.88 -1.00 6.76 -2.08 -7.17 4.44 3.39 8.32 -1.63
Vacuole -5.99 -0.53 -20.31 -9.68 -21.03 -8.94 -14.16 -15.66 -10.61 -10.47 -10.46 -11.53 -28.29 -16.45 -22.68
Vacuole Periphery -2.53 -1.54 -2.76 -0.91 -1.09 -4.47 0.15 -1.72 -0.60 -1.73 -5.01 -1.63 -3.45 -1.19 -2.38
Standard name
Human Ortholog
Description m(7)GpppX pyrophosphatase regulator; non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance; DCS2 has a paralog, DCS1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (44%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dcs2

Dcs2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dcs2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available