Standard name
Human Ortholog
Description RNA polymerase subunit ABC14.5; common to RNA polymerases I, II, and III
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0 – 0 0 0 0 0 0.06 0.11 0.11 0.12 0.08 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0 0 0 – 0.05 0.08 0.08 0.11 0.1 0 0 0 0 0.06 0 0 0 0 0 0 0
Nucleus 0.88 0.83 0.75 0.84 0.75 0.76 – 0.68 0.64 0.58 0.58 0.69 0.79 0.79 0.71 0.73 0.74 0.76 0.83 0.75 0.61 0.54 0.52 0.45
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.45 0.44 0.55 0.46 0.4 0.53 – 0.63 0.68 0.71 0.7 0.65 0.51 0.39 0.33 0.25 0.28 0.26 0.12 0.2 0.29 0.35 0.39 0.4
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 0 – 1 0 1 1 1 0 0 1 0 0 0 2 1 2 1 1 3
Bud Neck 0 1 0 0 1 0 – 1 0 3 1 1 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 1 0 0 1 0 2 – 8 4 7 8 9 0 0 1 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 7 11 10 12 6 – 11 5 8 2 2 17 28 36 27 11 16 0 1 1 5 2 2
Endoplasmic Reticulum 0 0 2 0 0 0 – 0 0 0 0 0 0 1 0 2 2 1 0 0 0 0 0 0
Endosome 0 0 0 0 4 1 – 0 0 0 0 0 2 3 8 3 2 2 1 0 6 0 1 4
Golgi 1 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 1 1 1 1 0 2 3
Mitochondria 5 4 17 6 7 8 – 20 20 27 33 36 0 1 0 7 9 4 0 1 2 0 1 2
Nucleus 219 178 212 177 166 210 – 286 162 208 177 251 230 206 223 161 104 145 210 167 182 88 126 130
Nuclear Periphery 0 0 0 0 0 2 – 3 4 2 0 4 0 0 1 0 1 0 0 0 1 0 0 1
Nucleolus 113 95 155 97 88 146 – 266 171 254 213 237 149 102 105 55 39 50 30 44 87 57 93 116
Peroxisomes 1 1 0 1 1 0 – 0 0 0 0 0 1 3 3 0 0 1 0 0 0 0 0 1
SpindlePole 1 0 0 0 0 1 – 0 0 1 0 0 2 2 10 0 0 1 0 0 2 0 1 3
Vac/Vac Membrane 0 3 0 0 2 1 – 1 1 0 1 1 1 0 4 3 3 5 5 4 5 7 8 19
Unique Cell Count 250 214 281 211 222 275 422 252 357 305 364 292 261 315 222 141 192 254 224 298 165 241 292
Labelled Cell Count 342 289 397 292 281 377 597 367 511 436 542 402 347 392 258 171 227 254 224 298 165 241 292


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.3 22.3 12.3 15.6 14.8 12.3 – 11.3 10.5 10.8 10.5 10.6 17.4 18.6 16.9 17.4 17.6 16.4 20.6 19.7 19.6
Std Deviation (1e-4) 4.5 7.6 4.2 4.9 5.3 3.4 – 3.4 3.7 3.1 3.0 3.5 6.0 5.8 6.1 6.4 6.0 5.8 11.4 6.1 5.4
Intensity Change (Log2) – – – 0.34 0.27 0.0 – -0.12 -0.22 -0.18 -0.23 -0.22 0.5 0.6 0.46 0.5 0.52 0.42 0.74 0.68 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 0.8 -1.2 – -1.0 -1.3 -1.2 -2.7 -3.0 1.1 3.1 3.4 3.5 1.7 2.0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.7 -1.5 -1.8 – -0.8 0.9 0.7 2.1 1.8 -4.3 -3.7 -4.4 -1.5 0.1 -2.1
Nucleus 2.3 -0.2 0.3 – -2.2 -2.8 -4.5 -4.5 -1.8 0.9 1.0 -1.3 -0.7 -0.4 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.0 -3.5 -0.5 – 2.1 3.0 4.2 3.7 2.6 -1.0 -3.7 -5.4 -6.9 -5.3 -6.3
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.1808 13.6742 12.497 11.6484 10.8139 12.1314 11.1813 11.0375 10.5618 10.372 8.7697 10.3675 14.9136 14.9744 15.0087 14.9088 13.5699 13.7176
Actin 0.0276 0 0.0126 0.0324 0.0013 0.0007 0.0055 0 0.0073 0 0.0128 0.0002 0.0059 0 0.0128 0 0 0.0002
Bud 0.0012 0 0.0003 0.0001 0.0004 0.0001 0.0009 0 0.0001 0 0.0003 0.0001 0.0003 0 0.0001 0 0 0
Bud Neck 0.0014 0 0.0001 0.0001 0.0002 0.0003 0.0004 0 0.0041 0 0.0004 0.0004 0.0003 0 0.0002 0 0.0001 0.0002
Bud Periphery 0.0015 0 0.0003 0.0002 0.0005 0.0001 0.0014 0 0.0002 0 0.0012 0.0001 0.0005 0 0.0002 0 0.0001 0
Bud Site 0.0043 0 0.0021 0.0003 0.0013 0.0002 0.0028 0 0.0016 0 0.001 0.0003 0.0006 0 0.0002 0 0 0
Cell Periphery 0.0004 0 0.0001 0 0.0004 0.0001 0.0004 0 0.0001 0 0.0013 0.0001 0.0004 0 0.0001 0 0.0001 0
Cytoplasm 0.0039 0.0001 0.0021 0.0001 0.0005 0.0001 0.0047 0 0.0002 0 0.002 0.0002 0.0012 0 0.0001 0 0 0.0001
Cytoplasmic Foci 0.0116 0 0.0079 0.0027 0.0271 0.0016 0.0174 0 0.0078 0 0.0142 0.0016 0.0003 0 0.0003 0 0 0.0001
Eisosomes 0.0003 0 0.0001 0.0002 0.0001 0 0.0001 0 0 0 0.0001 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0009 0 0.0011 0.0001 0.0002 0.0002 0.0027 0 0.0001 0 0.0007 0.0001 0.0018 0 0.0003 0 0 0.0002
Endosome 0.0131 0 0.012 0.0019 0.0043 0.0058 0.0227 0 0.0033 0 0.0114 0.0036 0.0026 0 0.0012 0 0 0.0015
Golgi 0.0072 0 0.0026 0.0045 0.0025 0.0021 0.0064 0 0.0025 0 0.0096 0.0004 0.0009 0 0.0007 0 0 0.0001
Lipid Particles 0.0107 0 0.0117 0.0032 0.0242 0.0043 0.0216 0 0.0096 0 0.0349 0.0015 0.0009 0 0.0003 0 0 0.0001
Mitochondria 0.0058 0.0002 0.0126 0.0081 0.012 0.0131 0.0096 0.0002 0.0016 0 0.0299 0.0015 0.0018 0.0001 0.0007 0 0.0009 0.0003
None 0.0049 0 0.001 0.0002 0.0012 0.0001 0.0029 0 0.0003 0 0.004 0.0002 0.0005 0 0.0004 0 0 0.0001
Nuclear Periphery 0.0151 0.0004 0.0038 0.0004 0.0018 0.0002 0.0115 0.0025 0.0009 0.0002 0.0024 0.0008 0.0068 0.0002 0.0007 0 0.0002 0.002
Nucleolus 0.1245 0.0473 0.0503 0.0694 0.4483 0.2422 0.1076 0.0638 0.0457 0.0496 0.4002 0.2523 0.1233 0.0649 0.0401 0.0497 0.3604 0.2551
Nucleus 0.7408 0.9516 0.8725 0.8629 0.4057 0.7259 0.7612 0.9323 0.901 0.9501 0.4378 0.7328 0.8474 0.9345 0.9409 0.9501 0.6377 0.7392
Peroxisomes 0.0077 0 0.0035 0.0115 0.0113 0.0017 0.0043 0 0.0099 0 0.01 0.0002 0.0001 0 0.0003 0 0 0
Punctate Nuclear 0.0126 0.0001 0.002 0.0014 0.0524 0.0004 0.0095 0.0001 0.0027 0.0001 0.0045 0.0009 0.0007 0.0002 0.0003 0 0.0003 0.0004
Vacuole 0.0027 0.0001 0.0006 0.0001 0.0016 0.0004 0.0037 0.0001 0.0004 0 0.0093 0.0019 0.0023 0 0.0001 0 0.0001 0.0002
Vacuole Periphery 0.0018 0.0001 0.0009 0.0003 0.0027 0.0004 0.0026 0.0008 0.0004 0 0.0121 0.0007 0.0013 0 0.0001 0 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 183.9007 219.1947 154.3534 170.8383 191.3971 117.5313 158.8708 152.6364 135.8458 160.4239
Translational Efficiency 1.3882 1.2286 1.6738 1.3091 1.212 2.0386 1.5167 1.284 1.5676 1.5217

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1624 1820 2138 1467 842 1911 1314 1375 2466 3731 3452 2842

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1313.90 1500.37 1651.35 1379.48 959.05 1487.74 1629.73 1586.22 1192.74 1493.90 1643.12 1479.50
Standard Deviation 272.62 352.84 323.33 305.16 236.60 316.81 362.35 358.98 310.44 334.93 338.88 347.98
Intensity Change Log 2 — 0.191463 0.329790 0.070269 — 0.633444 0.764955 0.725915 — 0.394667 0.529605 0.383807

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000525 0.000578 0.000706 0.001078 0.000664 0.000784 0.000706 0.001006 0.000573 0.000684 0.000706 0.001043
Bud Neck 0.002370 0.002913 0.007196 0.012244 0.002296 0.003671 0.005187 0.014183 0.002345 0.003301 0.006431 0.013182
Bud Site 0.000269 0.000358 0.000666 0.002119 0.000361 0.000845 0.000561 0.003477 0.000301 0.000607 0.000626 0.002776
Cell Periphery 0.000027 0.000036 0.000020 0.000076 0.000118 0.000084 0.000020 0.000180 0.000058 0.000060 0.000020 0.000126
Cytoplasm 0.000100 0.000081 0.000034 0.000668 0.000779 0.000118 0.000042 0.000780 0.000332 0.000100 0.000037 0.000722
Cytoplasmic Foci 0.000394 0.000206 0.000105 0.001375 0.000166 0.000492 0.000744 0.002099 0.000316 0.000352 0.000348 0.001725
Eisosomes 0.000196 0.000178 0.000189 0.000094 0.000096 0.000216 0.000191 0.000099 0.000162 0.000197 0.000190 0.000096
Endoplasmic Reticulum 0.000400 0.000348 0.000227 0.000601 0.000608 0.000426 0.000180 0.000425 0.000471 0.000388 0.000209 0.000516
Endosome 0.000014 0.000021 0.000006 0.001418 0.000034 0.000198 0.000396 0.001771 0.000021 0.000112 0.000154 0.001589
Golgi 0.000038 0.000087 0.000046 0.000794 0.000086 0.000146 0.000516 0.000657 0.000054 0.000117 0.000225 0.000728
Lipid Particles 0.000021 0.000131 0.000003 0.000329 0.000015 0.000271 0.000008 0.000237 0.000019 0.000203 0.000005 0.000285
Mitochondria 0.000858 0.000951 0.001387 0.003581 0.001067 0.001294 0.001972 0.003942 0.000929 0.001126 0.001610 0.003755
Mitotic Spindle 0.001037 0.001380 0.000172 0.004939 0.000370 0.007779 0.000236 0.010477 0.000809 0.004657 0.000196 0.007618
None 0.000621 0.000490 0.000541 0.002366 0.005789 0.000721 0.000560 0.001308 0.002386 0.000608 0.000548 0.001854
Nuclear Periphery 0.000026 0.000026 0.000028 0.000250 0.000033 0.000092 0.000039 0.000424 0.000029 0.000060 0.000032 0.000334
Nuclear Periphery Foci 0.000088 0.000101 0.000005 0.000440 0.000373 0.000228 0.000029 0.000374 0.000186 0.000166 0.000014 0.000409
Nucleolus 0.124823 0.117462 0.112732 0.137303 0.125833 0.152135 0.139736 0.107196 0.125168 0.135221 0.123011 0.122737
Nucleus 0.861739* 0.868104* 0.867580* 0.819695* 0.853747* 0.822507* 0.837353* 0.843306* 0.859011* 0.844749* 0.856074* 0.831118*
Peroxisomes 0.006024 0.006165 0.008034 0.007830 0.007221 0.007114 0.010393 0.004537 0.006433 0.006651 0.008932 0.006237
Vacuole 0.000192 0.000157 0.000104 0.001692 0.000190 0.000519 0.000357 0.002253 0.000191 0.000342 0.000200 0.001963
Vacuole Periphery 0.000236 0.000229 0.000221 0.001108 0.000154 0.000361 0.000776 0.001270 0.000208 0.000297 0.000432 0.001186

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.92 -7.09 -12.15 -8.28 -9.04 -0.82 -1.25 -3.88 -1.88 -3.39 -1.35 -6.11 -8.65 -4.51 -6.56
Bud Neck -3.44 -22.04 -13.72 -12.92 -7.28 -6.05 -12.00 -12.71 -11.33 -9.86 -6.98 -24.85 -18.42 -16.81 -11.92
Bud Site -1.68 -13.23 -4.85 -4.57 -3.97 -2.45 -1.78 -5.87 -4.99 -5.61 -3.23 -8.24 -7.68 -6.70 -6.83
Cell Periphery -0.42 0.78 -4.04 -2.23 -5.87 0.79 2.15 -1.04 -2.39 -4.55 0.05 2.28 -2.85 -3.05 -6.09
Cytoplasm -0.10 0.43 -3.65 -3.66 -3.85 2.02 2.17 0.13 -3.39 -3.67 1.88 2.20 -2.26 -4.97 -5.28
Cytoplasmic Foci 0.61 0.97 -1.18 -2.03 -2.35 -0.55 -0.86 -3.14 -2.93 -2.17 0.15 0.28 -2.79 -3.49 -3.49
Eisosomes 1.21 0.33 7.62 10.14 13.45 -7.91 -12.46 -0.47 7.71 12.01 -2.90 -2.93 7.12 11.55 17.43
Endoplasmic Reticulum 0.69 3.32 -2.95 -2.76 -9.83 2.48 7.27 1.61 -1.28 -9.16 1.43 6.17 -1.64 -3.11 -13.16
Endosome -0.97 1.70 -2.95 -2.93 -2.97 -2.04 -1.34 -3.22 -2.78 -2.25 -2.20 -1.29 -4.37 -3.99 -3.80
Golgi -0.94 0.10 -2.22 -2.06 -2.23 -1.16 -1.19 -3.20 -2.78 -0.36 -1.75 -1.19 -3.49 -3.11 -2.11
Lipid Particles -0.78 1.15 -1.91 -0.91 -2.29 -1.67 0.95 -3.10 0.10 -3.34 -1.75 1.32 -3.08 -0.57 -3.63
Mitochondria -1.08 -8.44 -4.23 -4.08 -3.34 -1.34 -2.69 -6.09 -5.53 -3.61 -2.14 -5.19 -7.12 -6.56 -5.16
Mitotic Spindle -0.70 1.50 -3.87 -3.18 -4.80 -4.42 0.42 -5.62 -1.33 -5.70 -3.97 1.55 -6.61 -2.70 -7.43
None 1.10 0.64 -3.55 -3.84 -3.74 3.09 3.20 2.66 -3.08 -6.12 3.12 3.25 0.49 -4.65 -5.02
Nuclear Periphery -0.03 -0.48 -7.38 -7.34 -7.36 -2.81 -0.70 -6.91 -5.70 -6.74 -2.70 -0.87 -9.72 -8.49 -9.57
Nuclear Periphery Foci -0.03 1.65 -1.70 -1.67 -2.35 1.07 1.84 0.29 -1.27 -2.74 0.71 2.34 -1.20 -2.07 -3.55
Nucleolus 1.40 2.01 -2.27 -3.49 -4.04 -4.24 -2.26 2.04 6.62 4.35 -2.53 0.11 -0.16 2.21 -0.26
Nucleus -1.07 -0.67 6.81 7.64 7.44 4.36 2.27 2.55 -1.35 0.43 3.26 1.05 6.77 4.05 5.94
Peroxisomes -0.13 -3.38 -2.60 -2.53 0.73 0.01 -4.27 4.46 4.72 8.32 -0.49 -5.33 0.38 0.91 5.85
Vacuole 0.65 1.78 -2.83 -2.91 -3.01 -1.00 -0.17 -3.33 -3.03 -3.30 -1.12 0.48 -4.44 -4.16 -4.54
Vacuole Periphery 0.33 0.95 -2.21 -2.23 -2.25 -4.26 -1.41 -3.91 -3.07 -0.85 -3.34 -1.34 -4.05 -3.61 -2.42
Standard name
Human Ortholog
Description RNA polymerase subunit ABC14.5; common to RNA polymerases I, II, and III
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Rpb8

Rpb8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpb8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available