Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial matrix cochaperone; nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; acts as oxidative sensor to regulate mitochondrial Ssc1p; in presence of oxidative stress, dimeric Mge1p becomes a monomer and unable to regulate Ssc1p function; homolog of E. coli GrpE and human Mge1 (GRPEL1), which also responds to oxidative stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 1 | 1 | 1 | 4 | 3 | 5 | 2 | 8 | 8 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 2 | 3 | 2 | 7 | 4 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 14 | 4 | 5 | 11 | 1 | 17 | 16 | 10 | 14 | 30 | 27 | 9 | 6 | 7 | 5 | 4 | 2 | 0 | 0 | 0 | 1 | 2 | 1 |
Cytoplasm | 182 | 256 | 76 | 77 | 181 | 79 | 174 | 224 | 119 | 127 | 245 | 246 | 184 | 261 | 368 | 194 | 124 | 183 | 198 | 252 | 95 | 194 | 290 | 339 |
Endoplasmic Reticulum | 3 | 4 | 3 | 1 | 9 | 2 | 7 | 4 | 7 | 1 | 17 | 13 | 4 | 2 | 2 | 9 | 2 | 5 | 3 | 2 | 0 | 6 | 8 | 9 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 2 | 0 | 0 | 0 | 0 | 26 | 16 | 39 | 41 | 54 | 103 | 111 | 3 | 0 | 1 | 4 | 2 | 4 | 3 | 0 | 0 | 2 | 6 | 1 |
Nucleus | 1 | 1 | 0 | 1 | 2 | 0 | 3 | 1 | 0 | 1 | 4 | 5 | 0 | 0 | 0 | 3 | 2 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 0 | 0 | 4 | 4 | 22 | 24 | 11 | 9 | 21 | 43 | 0 | 0 | 0 | 33 | 20 | 13 | 0 | 2 | 0 | 2 | 1 | 0 |
Unique Cell Count | 187 | 261 | 79 | 79 | 194 | 99 | 214 | 283 | 170 | 174 | 363 | 389 | 188 | 263 | 371 | 223 | 141 | 197 | 218 | 262 | 101 | 212 | 319 | 363 |
Labelled Cell Count | 193 | 277 | 83 | 85 | 209 | 115 | 246 | 313 | 197 | 212 | 437 | 460 | 200 | 269 | 378 | 255 | 155 | 215 | 218 | 262 | 101 | 212 | 319 | 363 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.6 | 9.5 | 8.8 | 8.4 | 9.5 | 5.8 | 8.2 | 7.8 | 7.2 | 7.3 | 7.3 | 7.4 | 9.4 | 10.0 | 10.0 | 12.4 | 12.0 | 12.5 | 8.1 | 8.6 | 9.6 |
Std Deviation (1e-4) | 1.8 | 1.3 | 1.3 | 1.2 | 1.6 | 1.2 | 1.8 | 1.6 | 1.6 | 1.6 | 1.4 | 1.5 | 1.4 | 1.5 | 1.7 | 4.1 | 3.8 | 3.6 | 1.3 | 1.5 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.11 | -0.6 | -0.1 | -0.17 | -0.29 | -0.27 | -0.28 | -0.26 | 0.09 | 0.18 | 0.19 | 0.49 | 0.44 | 0.51 | -0.12 | -0.03 | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0.8 | 0 | 0 | 0.9 | 1.0 | 0.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.5 | -0.9 | -3.2 | -3.2 | -3.6 | -4.7 | -4.3 | -5.2 | -5.8 | 0.8 | 2.0 | 2.1 | -2.3 | -2.0 | -1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.9 | 0 | 3.5 | 4.8 | 5.6 | 5.4 | 5.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 3.0 | 2.7 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 3.6 | 3.5 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.3682 | 3.5797 | 3.1111 | 2.9959 | 2.5889 | 2.98 | 4.1658 | 6.1213 | 5.8385 | 5.2309 | 5.4614 | 5.5903 | 5.9216 | 7.6581 | 7.0095 | 6.8446 | 6.3888 | 6.7624 |
Actin | 0.0143 | 0.0105 | 0.016 | 0.0038 | 0.0135 | 0.0018 | 0.0225 | 0.0004 | 0.0042 | 0.0032 | 0.009 | 0.0026 | 0.0012 | 0.0002 | 0.0043 | 0.0003 | 0.0018 | 0.0011 |
Bud | 0.0006 | 0.0007 | 0.0051 | 0.0005 | 0.0005 | 0.0002 | 0.0009 | 0.0021 | 0.0046 | 0.0011 | 0.0012 | 0.0004 | 0.0001 | 0.0003 | 0.0006 | 0.0005 | 0.0002 | 0.0001 |
Bud Neck | 0.0018 | 0.0003 | 0.0012 | 0.0004 | 0.0012 | 0.0013 | 0.0032 | 0.0007 | 0.0005 | 0.0005 | 0.0003 | 0.0021 | 0.0002 | 0.0001 | 0.0005 | 0.0003 | 0.0005 | 0.0015 |
Bud Periphery | 0.0006 | 0.0004 | 0.0055 | 0.0003 | 0.0002 | 0.0001 | 0.001 | 0.0005 | 0.0014 | 0.0011 | 0.0013 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 |
Bud Site | 0.0024 | 0.0015 | 0.0044 | 0.0009 | 0.0006 | 0.0001 | 0.003 | 0.0161 | 0.0032 | 0.0005 | 0.0004 | 0.0004 | 0.0003 | 0.0009 | 0.001 | 0.0005 | 0.0002 | 0.0001 |
Cell Periphery | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.7956 | 0.9039 | 0.8386 | 0.8741 | 0.7726 | 0.8953 | 0.7124 | 0.9394 | 0.9339 | 0.864 | 0.9157 | 0.9497 | 0.9078 | 0.9693 | 0.9359 | 0.8957 | 0.9177 | 0.9581 |
Cytoplasmic Foci | 0.0406 | 0.0163 | 0.0295 | 0.032 | 0.0425 | 0.0205 | 0.0304 | 0.0205 | 0.0119 | 0.0172 | 0.0079 | 0.0112 | 0.0133 | 0.0108 | 0.0166 | 0.0238 | 0.0227 | 0.0081 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0111 | 0.0044 | 0.0048 | 0.0049 | 0.0042 | 0.0114 | 0.0243 | 0.001 | 0.0019 | 0.0028 | 0.0073 | 0.0032 | 0.0216 | 0.0017 | 0.004 | 0.0037 | 0.0065 | 0.0068 |
Endosome | 0.0508 | 0.0186 | 0.0365 | 0.0465 | 0.0693 | 0.0303 | 0.0538 | 0.0103 | 0.0137 | 0.0548 | 0.0326 | 0.0122 | 0.0135 | 0.0046 | 0.0192 | 0.0591 | 0.0279 | 0.0137 |
Golgi | 0.0087 | 0.0054 | 0.0085 | 0.0107 | 0.0075 | 0.0035 | 0.014 | 0.0008 | 0.005 | 0.0086 | 0.0078 | 0.004 | 0.0027 | 0.0017 | 0.0033 | 0.0023 | 0.0028 | 0.0012 |
Lipid Particles | 0.0038 | 0.0004 | 0.007 | 0.0026 | 0.0227 | 0.0005 | 0.0151 | 0.0002 | 0.0006 | 0.0055 | 0.0005 | 0.0013 | 0.0016 | 0.0001 | 0.0002 | 0.0002 | 0.0013 | 0.0006 |
Mitochondria | 0.011 | 0.003 | 0.0061 | 0.003 | 0.0021 | 0.0014 | 0.0197 | 0.0001 | 0.0092 | 0.02 | 0.0064 | 0.0015 | 0.0006 | 0.0002 | 0.0005 | 0.0003 | 0.0004 | 0.0005 |
None | 0.0353 | 0.0276 | 0.0168 | 0.0059 | 0.0218 | 0.0274 | 0.0258 | 0.0048 | 0.0042 | 0.0036 | 0.0025 | 0.0035 | 0.0303 | 0.0073 | 0.0091 | 0.0038 | 0.0125 | 0.005 |
Nuclear Periphery | 0.004 | 0.0006 | 0.0018 | 0.0016 | 0.001 | 0.0007 | 0.0208 | 0.0003 | 0.0005 | 0.0013 | 0.0028 | 0.0006 | 0.0011 | 0.0002 | 0.0005 | 0.001 | 0.0008 | 0.0006 |
Nucleolus | 0.0008 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0023 | 0.0008 | 0.0009 | 0.0012 | 0.0009 | 0.0008 | 0.0163 | 0.0009 | 0.0009 | 0.0016 | 0.0009 | 0.0008 | 0.0009 | 0.0005 | 0.0007 | 0.0011 | 0.001 | 0.0007 |
Peroxisomes | 0.0082 | 0.0017 | 0.0107 | 0.0042 | 0.0267 | 0.001 | 0.0176 | 0.0002 | 0.0012 | 0.0014 | 0.0002 | 0.0024 | 0.0014 | 0.0006 | 0.0004 | 0.0001 | 0.0003 | 0.0001 |
Punctate Nuclear | 0.002 | 0.0003 | 0.0012 | 0.0009 | 0.0038 | 0.0002 | 0.0027 | 0.0003 | 0.0003 | 0.0005 | 0.0002 | 0.001 | 0.0007 | 0.0001 | 0.0002 | 0.0007 | 0.0004 | 0.0003 |
Vacuole | 0.0045 | 0.0029 | 0.004 | 0.005 | 0.007 | 0.0026 | 0.0091 | 0.0013 | 0.002 | 0.0094 | 0.0018 | 0.0019 | 0.0021 | 0.0012 | 0.0022 | 0.0051 | 0.0024 | 0.0011 |
Vacuole Periphery | 0.0012 | 0.0006 | 0.0011 | 0.0015 | 0.0016 | 0.0007 | 0.006 | 0.0002 | 0.0005 | 0.0027 | 0.0011 | 0.0007 | 0.0003 | 0.0001 | 0.0004 | 0.0012 | 0.0004 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 140.5355 | 122.0407 | 117.1946 | 120.8477 | 122.8619 | 121.9975 | 124.847 | 123.4555 | 131.9542 | 138.0746 |
Translational Efficiency | 1.8166 | 1.8993 | 1.9138 | 1.693 | 1.8964 | 2.3176 | 2.0007 | 1.9156 | 2.0127 | 1.9799 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1910 | 657 | 179 | 1215 | 1035 | 2677 | 924 | 1381 | 2945 | 3334 | 1103 | 2596 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 789.62 | 950.78 | 1318.87 | 1067.88 | 888.19 | 977.90 | 1118.09 | 1098.00 | 824.26 | 972.56 | 1150.67 | 1083.90 |
Standard Deviation | 114.91 | 146.28 | 186.23 | 161.29 | 105.74 | 126.43 | 158.58 | 158.57 | 121.28 | 131.03 | 179.38 | 160.56 |
Intensity Change Log 2 | — | 0.267953 | 0.740072 | 0.435519 | — | 0.138819 | 0.332096 | 0.305938 | — | 0.201034 | 0.538503 | 0.368374 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000341 | 0.000772 | 0.001977 | 0.002356 | 0.000347 | 0.000692 | 0.000524 | 0.000352 | 0.000343 | 0.000707 | 0.000760 | 0.001290 |
Bud Neck | 0.006824 | 0.003217 | 0.003843 | 0.003625 | 0.003623 | 0.004746 | 0.001675 | 0.002960 | 0.005699 | 0.004445 | 0.002027 | 0.003271 |
Bud Site | 0.020646 | 0.039936 | 0.031454 | 0.055014 | 0.020114 | 0.032032 | 0.022192 | 0.033041 | 0.020459 | 0.033590 | 0.023695 | 0.043325 |
Cell Periphery | 0.000118 | 0.000131 | 0.000290 | 0.000202 | 0.000191 | 0.000110 | 0.000131 | 0.000071 | 0.000144 | 0.000114 | 0.000156 | 0.000132 |
Cytoplasm | 0.667388* | 0.733880* | 0.607493* | 0.747288* | 0.685459* | 0.760551* | 0.831158* | 0.793432* | 0.673739* | 0.755295* | 0.794860* | 0.771835* |
Cytoplasmic Foci | 0.167085 | 0.139594 | 0.089642 | 0.059542 | 0.207553* | 0.115828 | 0.056529 | 0.056776 | 0.181307* | 0.120512 | 0.061903 | 0.058071 |
Eisosomes | 0.000056 | 0.000027 | 0.000029 | 0.000021 | 0.000067 | 0.000035 | 0.000010 | 0.000010 | 0.000060 | 0.000034 | 0.000013 | 0.000015 |
Endoplasmic Reticulum | 0.002486 | 0.000829 | 0.001655 | 0.000506 | 0.001730 | 0.000448 | 0.000205 | 0.000160 | 0.002220 | 0.000523 | 0.000440 | 0.000322 |
Endosome | 0.012595 | 0.011232 | 0.012971 | 0.007595 | 0.009591 | 0.007201 | 0.001978 | 0.003165 | 0.011540 | 0.007995 | 0.003762 | 0.005239 |
Golgi | 0.010319 | 0.004207 | 0.000990 | 0.007394 | 0.009986 | 0.011248 | 0.000355 | 0.000506 | 0.010202 | 0.009861 | 0.000458 | 0.003730 |
Lipid Particles | 0.002240 | 0.000543 | 0.003423 | 0.000751 | 0.001721 | 0.000700 | 0.000524 | 0.000352 | 0.002058 | 0.000669 | 0.000994 | 0.000539 |
Mitochondria | 0.002501 | 0.001265 | 0.000088 | 0.002273 | 0.001200 | 0.002097 | 0.000057 | 0.000064 | 0.002044 | 0.001933 | 0.000062 | 0.001098 |
Mitotic Spindle | 0.000526 | 0.000530 | 0.008480 | 0.011135 | 0.001503 | 0.005375 | 0.004293 | 0.006390 | 0.000870 | 0.004420 | 0.004972 | 0.008611 |
None | 0.001416 | 0.000401 | 0.001326 | 0.000583 | 0.000577 | 0.001747 | 0.002529 | 0.001759 | 0.001121 | 0.001481 | 0.002334 | 0.001209 |
Nuclear Periphery | 0.000151 | 0.000027 | 0.000495 | 0.000055 | 0.000065 | 0.000030 | 0.000062 | 0.000041 | 0.000120 | 0.000029 | 0.000133 | 0.000047 |
Nuclear Periphery Foci | 0.000304 | 0.000751 | 0.010231 | 0.001189 | 0.000534 | 0.000143 | 0.003338 | 0.001066 | 0.000385 | 0.000263 | 0.004457 | 0.001124 |
Nucleolus | 0.000329 | 0.000102 | 0.000118 | 0.000087 | 0.000110 | 0.000192 | 0.000096 | 0.000124 | 0.000252 | 0.000174 | 0.000099 | 0.000107 |
Nucleus | 0.023995 | 0.009441 | 0.053532 | 0.011013 | 0.017517 | 0.008690 | 0.021942 | 0.012292 | 0.021718 | 0.008838 | 0.027069 | 0.011693 |
Peroxisomes | 0.002107 | 0.002234 | 0.001559 | 0.003034 | 0.001198 | 0.002221 | 0.000985 | 0.001328 | 0.001787 | 0.002223 | 0.001079 | 0.002126 |
Vacuole | 0.073611 | 0.048773 | 0.169882 | 0.084021 | 0.034328 | 0.042389 | 0.051209 | 0.085836 | 0.059806 | 0.043647 | 0.070468 | 0.084987 |
Vacuole Periphery | 0.004961 | 0.002109 | 0.000522 | 0.002315 | 0.002585 | 0.003524 | 0.000209 | 0.000273 | 0.004126 | 0.003245 | 0.000260 | 0.001229 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.71 | -2.35 | -3.98 | -2.16 | -0.55 | -1.72 | -2.74 | 0.84 | 1.96 | 3.12 | -2.14 | -3.51 | -3.70 | -1.09 | -0.85 |
Bud Neck | 6.17 | 4.44 | 6.96 | 0.46 | 2.23 | -2.57 | 5.34 | 2.99 | 5.54 | -2.31 | 3.11 | 10.22 | 8.20 | 5.05 | -2.04 |
Bud Site | -5.89 | -0.77 | -5.35 | 0.56 | -2.56 | -6.13 | -0.33 | -2.28 | 2.21 | -1.78 | -8.51 | -0.49 | -5.43 | 0.96 | -3.98 |
Cell Periphery | -0.98 | -6.48 | -1.43 | -0.75 | 4.37 | 6.03 | 1.93 | 9.53 | 7.01 | 2.80 | 4.19 | -0.74 | 3.78 | 1.13 | 2.44 |
Cytoplasm | -5.82 | 5.07 | -3.38 | 2.70 | -6.52 | -9.12 | -11.54 | -8.04 | 0.00 | 3.90 | -13.64 | -10.54 | -8.83 | 3.74 | 3.23 |
Cytoplasmic Foci | 3.97 | 12.36 | 24.39 | 13.51 | 2.31 | 14.67 | 24.69 | 25.29 | 17.69 | 0.27 | 14.88 | 30.56 | 34.78 | 22.56 | 1.00 |
Eisosomes | 4.13 | 6.58 | 9.33 | 1.00 | 2.26 | 4.42 | 8.50 | 8.76 | 7.55 | 1.73 | 6.07 | 13.63 | 13.48 | 6.26 | -0.85 |
Endoplasmic Reticulum | 2.95 | 2.03 | 4.22 | 1.55 | 4.34 | 4.51 | 5.35 | 5.68 | 4.35 | 3.16 | 5.01 | 5.37 | 5.88 | 2.81 | 2.50 |
Endosome | 0.94 | 2.20 | 7.75 | 4.05 | 3.08 | 2.47 | 8.61 | 7.32 | 9.61 | -3.04 | 5.44 | 14.80 | 12.06 | 7.52 | -1.08 |
Golgi | 8.13 | 16.42 | 5.27 | 0.35 | -2.58 | -1.01 | 9.74 | 9.61 | 13.77 | -0.79 | 0.41 | 19.30 | 11.28 | 9.57 | -2.94 |
Lipid Particles | 7.96 | -2.65 | 4.87 | -3.20 | 5.27 | 7.19 | 7.19 | 9.93 | 5.10 | 6.24 | 9.43 | 5.24 | 8.77 | -0.31 | 4.36 |
Mitochondria | 1.62 | 9.44 | 1.64 | 0.10 | -1.28 | -1.73 | 2.91 | 2.92 | 6.02 | 0.18 | 0.30 | 9.21 | 3.46 | 2.84 | -1.32 |
Mitotic Spindle | -0.09 | -3.43 | -4.48 | -4.47 | 0.98 | -3.09 | -2.16 | -2.50 | -0.42 | -0.57 | -4.26 | -4.04 | -5.11 | -2.02 | -0.66 |
None | 4.03 | 0.62 | 2.82 | -3.35 | 2.55 | -5.16 | -2.68 | -2.72 | 0.65 | 0.99 | -1.32 | -1.64 | 0.15 | 1.44 | 1.70 |
Nuclear Periphery | 3.04 | -7.87 | 0.93 | -9.12 | 9.78 | 2.31 | -3.21 | -0.70 | -7.05 | 3.87 | 3.43 | -3.49 | 1.01 | -10.74 | 8.00 |
Nuclear Periphery Foci | -2.50 | -8.93 | -10.86 | -3.68 | 7.87 | 5.44 | -11.77 | -5.12 | -8.98 | 8.24 | 2.38 | -14.83 | -10.80 | -12.63 | 11.12 |
Nucleolus | 3.48 | 3.06 | 3.05 | -0.47 | 0.55 | -2.85 | 0.85 | -1.44 | 2.26 | -2.77 | 1.81 | 3.50 | 2.98 | 1.92 | -1.14 |
Nucleus | 8.29 | -6.80 | 3.55 | -6.06 | 7.93 | 7.20 | -7.19 | 0.89 | -8.75 | 8.38 | 12.00 | -7.85 | 4.00 | -11.53 | 10.83 |
Peroxisomes | -0.29 | 3.00 | -0.15 | 0.09 | -2.36 | -5.70 | 1.86 | 1.06 | 6.12 | -1.02 | -2.43 | 4.83 | 1.04 | 2.84 | -2.95 |
Vacuole | 4.67 | -9.10 | -13.85 | -15.83 | 3.74 | -3.38 | -10.11 | -18.71 | -17.61 | -8.10 | 7.06 | -8.45 | -20.21 | -25.54 | -7.05 |
Vacuole Periphery | 4.90 | 9.73 | 6.06 | 1.66 | -1.84 | -1.98 | 7.25 | 7.11 | 9.63 | -1.07 | 2.04 | 12.02 | 9.13 | 7.48 | -2.31 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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