ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.96 0.97 1.0 0.93 0.92 0.88 0.85 0.74 0.69 0.65 0.62 1.0 0.99 0.99 0.98 0.98 0.99 0.91 0.92 0.9 0.91 0.94 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.1 0.13 0.14 0.4 0.45 0.51 0.49 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.08 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 2 3 1 2 0 1
Bud 0 2 0 0 0 1 0 1 1 3 2 1 0 0 0 0 0 0 1 2 0 0 3 4
Bud Neck 0 1 2 0 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 2
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 2 0 0 3 0 2 0 1 0 0 1 2 0 1 9 4 0 0 0 0 0 0
Cytoplasm 267 451 100 118 78 119 141 189 70 68 48 39 290 394 624 231 274 267 256 445 109 230 312 317
Endoplasmic Reticulum 2 3 1 0 0 0 0 0 0 0 0 1 0 2 2 12 14 13 0 0 1 2 1 4
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 2 1 1 0 0 2 4 0 0 1 4
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 1 1 0 1 3
Mitochondria 0 13 0 0 4 13 21 32 38 44 38 31 0 0 0 4 5 3 2 11 3 1 1 1
Nucleus 1 2 2 2 5 3 4 1 0 2 1 2 0 0 1 1 3 1 1 1 0 0 1 1
Nuclear Periphery 1 0 0 0 0 0 2 0 1 0 0 0 0 0 0 1 0 0 0 1 0 2 1 1
Nucleolus 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 3 2 0 1 1 1
Vac/Vac Membrane 2 3 0 0 0 0 6 18 4 2 3 4 3 3 3 3 8 6 5 5 0 1 0 3
Unique Cell Count 271 468 103 118 84 129 161 223 94 98 74 63 291 396 629 236 281 270 281 487 122 252 332 359
Labelled Cell Count 274 477 107 120 88 139 178 244 115 120 94 79 294 403 631 255 315 295 281 487 122 252 332 359


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 4.7 6.5 5.7 4.7 5.3 5.1 5.5 4.5 4.6 4.8 5.9 6.4 6.7 6.0 5.6 6.8 6.8 6.9 7.3 7.9
Std Deviation (1e-4) 1.0 0.8 1.8 0.7 0.9 1.1 0.9 1.1 1.2 1.2 1.9 5.3 1.1 1.1 1.0 1.3 1.7 1.5 1.8 1.4 1.8
Intensity Change (Log2) – – – -0.2 -0.46 -0.3 -0.34 -0.25 -0.52 -0.49 -0.45 -0.15 -0.03 0.04 -0.11 -0.21 0.06 0.06 0.08 0.17 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5957 4.1509 3.9208 3.3381 3.7436 4.3518 5.9561 6.5677 6.7101 5.8942 5.7244 6.3316 3.5492 3.4494 3.4236 3.0337 2.6562 3.3532
Actin 0.0339 0.0008 0.0222 0.0068 0.0053 0.0142 0.0676 0.0007 0.0265 0.0075 0.0137 0.002 0.0289 0.0055 0.0232 0.0282 0.0811 0.0113
Bud 0.0002 0.0094 0.0004 0.0012 0.0003 0.0058 0.0052 0.0003 0.0034 0.0149 0.0049 0.0002 0.0032 0.0002 0.0024 0.0002 0.0018 0.0007
Bud Neck 0.0003 0.0001 0.0007 0.0026 0.001 0.0036 0.0013 0.0001 0.001 0.0006 0.0014 0.0006 0.0064 0.0002 0.0005 0.0073 0.004 0.0009
Bud Periphery 0.0003 0.0029 0.0004 0.0016 0.0004 0.0027 0.0145 0.0004 0.0055 0.0412 0.0067 0.0002 0.007 0.0001 0.0036 0.0002 0.0023 0.0014
Bud Site 0.002 0.0044 0.0023 0.0362 0.0007 0.0009 0.0051 0.0029 0.0024 0.0038 0.0151 0.0001 0.0175 0.0018 0.002 0.0013 0.0041 0.0002
Cell Periphery 0.0002 0.0004 0.0003 0.0005 0.0002 0.0001 0.0004 0.0003 0.0016 0.0004 0.0006 0.0001 0.0006 0.0002 0.0002 0.0001 0.0002 0.0001
Cytoplasm 0.4535 0.7594 0.694 0.7195 0.4646 0.6114 0.4176 0.7948 0.6651 0.6776 0.7003 0.729 0.2786 0.6737 0.5901 0.4691 0.5163 0.6863
Cytoplasmic Foci 0.0266 0.0031 0.0096 0.0087 0.0231 0.0297 0.0259 0.0026 0.0134 0.0097 0.0142 0.011 0.0361 0.005 0.0174 0.0282 0.041 0.0057
Eisosomes 0.0008 0 0.0003 0.0002 0.0001 0.0001 0.0005 0.0002 0.0022 0.0001 0.0002 0.0001 0.0004 0.0001 0.0003 0.0002 0.0003 0
Endoplasmic Reticulum 0.0025 0.0015 0.0031 0.0023 0.0012 0.0036 0.0045 0.0019 0.0072 0.0024 0.0188 0.0031 0.0139 0.0017 0.0029 0.002 0.0024 0.0012
Endosome 0.0139 0.001 0.012 0.0028 0.0904 0.0195 0.0487 0.0015 0.0108 0.0165 0.031 0.0034 0.0445 0.0018 0.0131 0.0038 0.013 0.0013
Golgi 0.0081 0.0001 0.0081 0.0011 0.0756 0.0105 0.0283 0.0006 0.0087 0.002 0.0199 0.0008 0.0191 0.0034 0.0051 0.0106 0.0199 0.0006
Lipid Particles 0.022 0 0.0036 0.0028 0.014 0.0105 0.0176 0.0002 0.0012 0.0243 0.0053 0.0006 0.0203 0.0002 0.0022 0.0135 0.0182 0.0002
Mitochondria 0.0093 0.0002 0.0141 0.0028 0.0437 0.0065 0.0176 0.0253 0.0051 0.0039 0.0387 0.0044 0.0309 0.0011 0.0036 0.0018 0.0058 0.0002
None 0.3823 0.2127 0.2199 0.1973 0.2583 0.2532 0.3139 0.1342 0.223 0.1754 0.1049 0.2343 0.4094 0.3013 0.3076 0.3574 0.2186 0.286
Nuclear Periphery 0.003 0.0005 0.0008 0.001 0.0004 0.0054 0.0034 0.0005 0.01 0.0013 0.0053 0.001 0.0289 0.0005 0.0039 0.0003 0.0007 0.0003
Nucleolus 0.0016 0.0001 0.0001 0.0005 0.0002 0.0002 0.0017 0.0215 0.0004 0.002 0.0013 0.0001 0.0034 0.0001 0.0003 0.0001 0.0002 0
Nucleus 0.003 0.0022 0.0014 0.0044 0.0008 0.0032 0.0022 0.0101 0.005 0.0057 0.0031 0.0018 0.0129 0.0015 0.0058 0.0005 0.0008 0.0017
Peroxisomes 0.0184 0.0001 0.0042 0.0051 0.0062 0.014 0.0081 0.0002 0.0024 0.0042 0.0042 0.0057 0.0145 0.0006 0.0104 0.0729 0.0661 0.0007
Punctate Nuclear 0.0158 0.0005 0.0005 0.0014 0.0003 0.0025 0.0106 0.0009 0.0027 0.0015 0.0009 0.0008 0.0133 0.0004 0.0015 0.0018 0.0023 0.0006
Vacuole 0.0016 0.0006 0.0015 0.0012 0.0081 0.0016 0.0035 0.0007 0.0016 0.0044 0.0066 0.0005 0.0059 0.0006 0.0026 0.0003 0.0006 0.0003
Vacuole Periphery 0.0005 0.0001 0.0004 0.0002 0.0052 0.0008 0.0017 0.0002 0.0009 0.0006 0.0027 0.0003 0.0046 0.0001 0.0015 0.0001 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.3333 8.6524 10.4333 9.2935 14.1179 15.7196 9.842 11.0712 8.6686 14.9872
Translational Efficiency 1.4349 1.5576 0.5024 0.5496 0.9442 1.255 0.9349 0.6534 0.7605 1.0358

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2419 1629 2405 84 1837 2529 171 1812 4256 4158 2576 1896

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 750.10 866.96 902.94 965.43 733.12 909.56 962.15 935.15 742.77 892.87 906.87 936.49
Standard Deviation 107.74 111.43 114.10 132.78 97.59 114.56 118.26 136.16 103.82 115.23 115.33 136.16
Intensity Change Log 2 — 0.208882 0.267547 0.364089 — 0.311119 0.392212 0.351148 — 0.260321 0.330513 0.357707

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000054 0.000337 0.000146 0.000523 0.000117 0.000588 0.000667 0.001009 0.000081 0.000490 0.000181 0.000988
Bud Neck 0.006045 0.010440 0.005518 0.001896 0.003590 0.016888 0.004959 0.007171 0.004986 0.014362 0.005481 0.006937
Bud Site 0.002140 0.014381 0.011623 0.009052 0.003042 0.016513 0.008576 0.041393 0.002529 0.015677 0.011420 0.039960
Cell Periphery 0.000095 0.000100 0.000081 0.000263 0.000088 0.000196 0.000301 0.000118 0.000092 0.000158 0.000096 0.000125
Cytoplasm 0.634716* 0.639675* 0.524239* 0.432941* 0.684303* 0.641857* 0.428023* 0.639108* 0.656119* 0.641002* 0.517852* 0.629974*
Cytoplasmic Foci 0.121150 0.136487 0.004683 0.031148 0.112140 0.103920 0.020414 0.013990 0.117261 0.116679 0.005727 0.014750
Eisosomes 0.000123 0.000043 0.000014 0.000100 0.000046 0.000049 0.000093 0.000020 0.000090 0.000047 0.000019 0.000024
Endoplasmic Reticulum 0.000647 0.001387 0.001326 0.005118 0.000483 0.000807 0.007180 0.001099 0.000576 0.001034 0.001715 0.001277
Endosome 0.004595 0.009508 0.000855 0.006827 0.004595 0.008153 0.006500 0.002684 0.004595 0.008684 0.001229 0.002868
Golgi 0.000913 0.002353 0.000061 0.000127 0.001195 0.001359 0.000160 0.000987 0.001035 0.001748 0.000067 0.000949
Lipid Particles 0.003074 0.001946 0.000425 0.003539 0.002369 0.001632 0.003375 0.000477 0.002770 0.001755 0.000620 0.000613
Mitochondria 0.001371 0.002425 0.000974 0.000132 0.001834 0.002771 0.000385 0.001313 0.001570 0.002635 0.000934 0.001260
Mitotic Spindle 0.001590 0.006239 0.004625 0.001248 0.002120 0.007902 0.001822 0.048268 0.001819 0.007251 0.004439 0.046185
None 0.002613 0.003302 0.006567 0.001235 0.005164 0.003225 0.005061 0.008430 0.003714 0.003255 0.006467 0.008112
Nuclear Periphery 0.000275 0.000292 0.001323 0.001234 0.000239 0.000259 0.001688 0.000751 0.000259 0.000272 0.001347 0.000772
Nuclear Periphery Foci 0.000303 0.001012 0.000466 0.009093 0.000539 0.000190 0.007359 0.000874 0.000405 0.000512 0.000923 0.001239
Nucleolus 0.000501 0.000628 0.000288 0.000256 0.000901 0.000722 0.000764 0.000440 0.000674 0.000686 0.000320 0.000432
Nucleus 0.169331* 0.100091 0.405118* 0.142487* 0.143093* 0.120674 0.288410* 0.157127* 0.158006* 0.112610 0.397370* 0.156478*
Peroxisomes 0.001240 0.003094 0.000150 0.000350 0.001419 0.003412 0.000455 0.000829 0.001317 0.003288 0.000170 0.000808
Vacuole 0.048782 0.064236 0.031363 0.352263* 0.032228 0.067572 0.213505* 0.072484 0.041637 0.066265 0.043454 0.084879
Vacuole Periphery 0.000445 0.002022 0.000157 0.000167 0.000494 0.001312 0.000305 0.001427 0.000466 0.001590 0.000167 0.001371

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.52 -7.61 -5.38 -2.27 -4.15 -1.29 -5.99 -3.17 0.07 0.47 -1.84 -6.97 -3.68 -0.28 -2.62
Bud Neck -4.48 1.18 5.93 7.96 3.50 -11.80 -0.66 -3.15 8.95 -0.93 -11.81 -0.23 -0.96 9.23 -0.58
Bud Site -9.48 -5.50 -2.22 0.69 -0.64 -9.28 -2.63 -7.40 -0.82 -3.65 -13.14 -5.44 -7.97 -1.16 -4.31
Cell Periphery -0.66 3.05 -3.56 -3.39 -4.16 -1.10 -5.19 0.83 1.16 5.33 -1.11 0.90 0.60 1.17 -0.17
Cytoplasm -0.32 26.50 10.39 10.35 3.86 5.75 16.79 19.34 14.17 -8.90 3.13 34.13 19.75 16.66 -9.20
Cytoplasmic Foci -2.97 40.93 27.22 24.84 -5.32 1.91 25.49 29.06 31.59 2.30 0.30 50.85 46.35 42.84 -8.62
Eisosomes 8.98 12.25 2.62 -5.53 -8.58 -1.03 -6.24 7.95 7.81 8.69 8.20 12.97 11.91 7.04 -2.63
Endoplasmic Reticulum -3.05 -13.29 -5.88 -4.61 -3.77 -1.45 -8.17 -7.78 -1.97 7.19 -2.77 -16.91 -8.09 -2.08 7.40
Endosome -6.08 15.17 1.35 5.88 -4.65 -5.37 -1.49 7.97 12.02 7.19 -8.24 15.00 12.14 15.31 -1.21
Golgi -4.03 5.82 5.36 7.06 -5.14 -0.42 3.08 2.44 5.07 -1.91 -2.94 5.79 3.73 7.54 -2.84
Lipid Particles 4.22 13.41 1.32 -1.64 -5.95 2.92 -1.36 7.58 4.78 6.40 5.54 14.42 13.35 6.47 -3.35
Mitochondria -1.75 1.39 4.01 4.56 5.89 -1.40 2.63 1.73 5.18 -3.41 -2.51 2.15 2.41 5.83 0.48
Mitotic Spindle -2.82 -3.99 -1.24 1.57 1.44 -3.92 0.34 -8.62 -6.21 -8.81 -5.03 -3.86 -8.94 -6.56 -7.65
None -1.07 -7.62 4.30 3.44 11.30 2.98 1.02 -3.19 -7.04 -3.88 1.16 -4.88 -6.76 -7.27 -2.33
Nuclear Periphery -0.77 -32.68 -8.22 -7.94 3.22 -0.77 -12.77 -23.48 -23.42 3.04 -0.84 -34.85 -24.88 -24.16 4.61
Nuclear Periphery Foci -7.95 -6.78 -6.62 -5.84 -6.23 4.81 -7.95 -3.79 -11.79 7.46 -1.85 -7.80 -9.18 -8.05 -1.47
Nucleolus -1.10 2.11 3.85 7.58 2.92 0.66 1.24 1.81 3.64 0.90 -0.03 2.74 2.06 4.22 -1.40
Nucleus 15.87 -45.36 1.90 -2.56 19.69 4.88 -7.21 -13.91 -18.86 3.10 14.27 -48.93 -12.28 -21.64 32.40
Peroxisomes -4.21 4.31 3.95 8.13 -2.01 -3.82 3.31 3.26 7.38 -0.05 -5.61 5.75 4.65 10.08 -2.39
Vacuole -6.09 -4.79 -13.51 -12.90 -13.03 -13.23 -12.86 -26.47 -18.76 6.23 -12.95 -11.78 -27.02 -21.56 -17.85
Vacuole Periphery -4.01 6.07 6.69 4.89 0.86 -4.52 3.11 0.14 3.63 -1.44 -5.99 7.34 0.08 4.74 -2.02
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
Localization
Cell Percentages cytoplasm (80%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YOR238W

YOR238W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yor238w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available