ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0.08 0.09 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0.05 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0 0 0
Cytoplasm 0.38 0.54 0.5 0.69 0.66 0.8 – 0.75 0.83 0.82 0.85 0.87 0.72 0.71 0.8 0.25 0.36 0.3 0.06 0.06 0.12 0.07 0.09 0.09
Endoplasmic Reticulum 0.09 0 0.05 0 0.05 0.05 – 0 0 0 0 0 0.14 0.19 0 0 0.06 0 0 0 0 0 0.07 0.07
Endosome 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.15 0.09 0 0.05 – 0.05 0.12 0.09 0.14 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.09 0.05 0.1 0 0 0 – 0 0 0 0 0 0 0 0 0.17 0.2 0.17 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.5 0.48 0.15 0.22 0.32 0.16 – 0.18 0.13 0.11 0.09 0.08 0.14 0 0 0.63 0.47 0.53 0.8 0.81 0.76 0.71 0.69 0.7
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 0 0 0 1 7 – 5 2 3 0 2 2 36 49 0 3 1 3 1 0 1 7 8
Bud 0 1 1 4 4 5 – 21 17 19 10 22 2 12 19 0 0 0 17 5 2 4 16 15
Bud Neck 0 0 0 0 1 0 – 1 0 1 0 1 0 0 2 0 0 0 2 1 0 1 4 9
Bud Site 0 0 0 0 0 0 – 0 1 0 3 2 0 3 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 3 2 – 1 3 3 1 3 2 26 30 0 0 0 0 0 0 0 1 2
Cytoplasm 104 117 10 161 279 298 – 639 427 511 368 450 135 324 411 21 38 21 19 13 8 4 34 37
Endoplasmic Reticulum 24 4 1 4 19 20 – 18 2 6 3 0 26 89 23 3 6 3 5 1 0 1 27 28
Endosome 4 11 0 4 5 0 – 1 1 1 0 0 3 0 4 2 1 3 9 10 2 3 2 6
Golgi 2 0 0 0 0 0 – 0 0 0 0 0 0 1 0 1 1 2 4 1 0 0 2 2
Mitochondria 10 4 3 21 15 18 – 46 62 58 59 65 2 3 1 1 2 3 3 3 0 0 13 9
Nucleus 26 10 2 10 11 3 – 17 3 11 5 5 0 0 0 14 21 12 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 1 – 5 5 6 6 6 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 – 1 3 1 4 3 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 – 0 0 1 1 0 0 0 5 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 – 6 4 3 3 3 1 5 21 1 0 1 1 0 0 0 2 2
Vac/Vac Membrane 139 103 3 52 133 58 – 153 69 70 38 40 26 14 8 52 49 37 265 189 53 50 252 292
Unique Cell Count 276 215 20 234 421 371 847 514 624 433 520 187 458 516 83 105 70 333 233 70 70 366 419
Labelled Cell Count 314 251 20 256 473 413 914 599 694 501 602 199 513 573 95 121 84 333 233 70 70 366 419


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 6.5 4.6 4.5 5.7 4.9 – 5.0 4.4 4.4 4.5 4.0 6.5 6.3 5.8 9.3 8.7 9.3 5.9 6.8 6.5
Std Deviation (1e-4) 1.3 2.2 0.8 1.3 1.4 1.3 – 1.5 1.6 1.3 7.1 1.1 1.7 1.3 1.4 2.6 2.2 2.6 1.2 1.8 1.6
Intensity Change (Log2) – – – -0.06 0.3 0.09 – 0.1 -0.08 -0.07 -0.03 -0.22 0.48 0.45 0.33 1.0 0.9 1.0 0.35 0.55 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.7 1.5 3.2 – 2.6 3.8 3.6 4.1 4.5 2.1 2.0 3.2 -2.2 -1.2 -1.7
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.6 0 – 0 0 0 0 0 0 0 0 3.8 2.6 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.85 1.6601 1.7233 1.5794 1.6513 1.6833 0.5504 2.2807 2.2141 1.4615 1.6369 1.9725 1.6294 3.589 3.748 3.0298 2.5923 3.1013
Actin 0.072 0 0.0185 0.0175 0.0354 0.002 0.0323 0.0008 0.0094 0.0016 0.0001 0.0078 0.0504 0.0001 0.002 0.0051 0.04 0.0116
Bud 0.0007 0.0003 0.0008 0.0004 0.0006 0.0008 0.0012 0.0008 0.0012 0.0005 0.0011 0.0005 0.0009 0.0008 0.0005 0.0006 0.0003 0.0004
Bud Neck 0.0025 0.0014 0.0065 0.0011 0.0031 0.0053 0.0013 0.003 0.0027 0.0011 0.0019 0.0031 0.0038 0.0028 0.0018 0.0014 0.0008 0.0017
Bud Periphery 0.0016 0.0008 0.0018 0.0008 0.0013 0.0029 0.0026 0.0019 0.002 0.0009 0.0025 0.0017 0.0016 0.0014 0.001 0.0008 0.0005 0.0012
Bud Site 0.0025 0.0019 0.0065 0.0024 0.0016 0.0012 0.0056 0.0052 0.0052 0.0008 0.001 0.0011 0.0031 0.0027 0.0068 0.0041 0.0008 0.0008
Cell Periphery 0.0028 0.003 0.0021 0.0027 0.0021 0.0033 0.0048 0.0053 0.0036 0.0016 0.0043 0.0081 0.001 0.0023 0.0011 0.0009 0.0004 0.0021
Cytoplasm 0.0337 0.0226 0.0236 0.0041 0.0055 0.0289 0.0454 0.0434 0.023 0.0608 0.0258 0.0242 0.0765 0.0797 0.0226 0.0041 0.0067 0.0155
Cytoplasmic Foci 0.0335 0.0168 0.0226 0.0129 0.0086 0.0144 0.0496 0.0265 0.0403 0.0266 0.0782 0.0212 0.038 0.015 0.0234 0.0299 0.0136 0.0083
Eisosomes 0.0005 0 0.0001 0.0001 0.0002 0 0.0001 0 0.0001 0 0 0.0001 0.0003 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0123 0.01 0.0057 0.0015 0.0054 0.0054 0.0135 0.0215 0.0141 0.0042 0.0072 0.0071 0.0202 0.0167 0.0083 0.004 0.002 0.009
Endosome 0.2542 0.161 0.2608 0.1417 0.2064 0.1914 0.3591 0.2236 0.3805 0.3306 0.3136 0.1916 0.3309 0.1779 0.3128 0.3943 0.2577 0.1416
Golgi 0.0113 0.0038 0.0101 0.0053 0.0405 0.0052 0.0248 0.0082 0.0329 0.0303 0.0223 0.0104 0.0152 0.0053 0.012 0.0285 0.0129 0.0168
Lipid Particles 0.0234 0.0056 0.0119 0.0174 0.0204 0.0078 0.0344 0.013 0.0252 0.0041 0.0077 0.0093 0.0375 0.0073 0.0183 0.0103 0.0081 0.0068
Mitochondria 0.007 0.0017 0.0214 0.0056 0.011 0.0156 0.014 0.0049 0.0181 0.0048 0.0071 0.0106 0.0152 0.002 0.03 0.01 0.0014 0.003
None 0.0966 0.016 0.0024 0.0041 0.001 0.0102 0.0205 0.0237 0.0185 0.0018 0.0017 0.0007 0.0518 0.019 0.0023 0.0005 0.0006 0.0002
Nuclear Periphery 0.016 0.0081 0.0051 0.0031 0.0341 0.0139 0.009 0.0095 0.0049 0.0024 0.0022 0.0032 0.0158 0.0116 0.007 0.0035 0.0038 0.0066
Nucleolus 0.0017 0.0022 0.0032 0.0025 0.002 0.0031 0.0017 0.0016 0.0012 0.0005 0.0005 0.0095 0.0016 0.0016 0.0018 0.0009 0.0006 0.001
Nucleus 0.0053 0.0041 0.0054 0.0023 0.0082 0.0159 0.0028 0.0035 0.009 0.0019 0.0012 0.0063 0.0041 0.0043 0.0054 0.0014 0.0013 0.0128
Peroxisomes 0.0194 0.0005 0.0028 0.0561 0.0028 0.0012 0.0079 0.0006 0.0024 0.0008 0.0022 0.0022 0.0041 0.0004 0.0045 0.0061 0.0034 0.006
Punctate Nuclear 0.006 0.0004 0.0026 0.001 0.0027 0.0055 0.0066 0.0003 0.0009 0.0002 0.0006 0.0005 0.0036 0.0003 0.0081 0.0005 0.0027 0.0004
Vacuole 0.3318 0.6528 0.4876 0.6537 0.5567 0.5931 0.3376 0.5669 0.3576 0.4689 0.4674 0.6285 0.2736 0.5887 0.4302 0.3885 0.555 0.6568
Vacuole Periphery 0.0652 0.0867 0.0984 0.0637 0.0503 0.0729 0.0251 0.0359 0.0472 0.0555 0.0511 0.0523 0.0507 0.0602 0.1002 0.1043 0.0876 0.0973

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 234.8831 143.2361 79.7155 98.4768 247.8783 134.6646 245.2166 167.7262 78.2747 294.6677
Translational Efficiency 1.307 1.5221 0.9427 0.2972 1.1465 1.3534 0.761 0.5631 0.7504 0.6164

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
148 1412 1418 100 227 1113 758 993 375 2525 2176 1093

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 697.25 761.15 923.00 899.26 845.11 823.86 943.41 838.87 786.75 788.79 930.11 844.40
Standard Deviation 106.00 113.20 115.41 121.23 104.46 106.33 137.65 121.50 127.53 114.54 123.99 122.72
Intensity Change Log 2 — 0.126505 0.404655 0.367062 — -0.036740 0.158746 -0.010692 — 0.039352 0.275126 0.172396

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.009779 0.001033 0.175260* 0.046320 0.004325 0.000691 0.220833* 0.063235 0.006478 0.000882 0.191135* 0.061687
Bud Neck 0.145828 0.037247 0.036890 0.013283 0.155641 0.047548 0.045073 0.024506 0.151768 0.041788 0.039741 0.023480
Bud Site 0.086655 0.044628 0.079524 0.067896 0.054503 0.029076 0.100433* 0.082478 0.067192 0.037773 0.086808 0.081144
Cell Periphery 0.068323 0.002101 0.022847 0.007281 0.192698* 0.003739 0.096222 0.004980 0.143611* 0.002823 0.048407 0.005190
Cytoplasm 0.136377* 0.109266* 0.253449* 0.221501* 0.084941 0.083885 0.167019* 0.337521* 0.105241* 0.098078 0.223342* 0.326906*
Cytoplasmic Foci 0.032602 0.053542 0.066498 0.113356 0.007811 0.031142 0.029343 0.118932 0.017596 0.043669 0.053555 0.118422
Eisosomes 0.007958 0.000139 0.033187 0.007896 0.003966 0.000264 0.061484 0.008073 0.005541 0.000194 0.043044 0.008056
Endoplasmic Reticulum 0.007004 0.004551 0.059638 0.027233 0.018198 0.006186 0.055749 0.015082 0.013780 0.005272 0.058284 0.016194
Endosome 0.044099 0.099531 0.017726 0.012324 0.016443 0.066700 0.021842 0.019100 0.027358 0.085059 0.019160 0.018480
Golgi 0.005747 0.006687 0.016332 0.003856 0.003183 0.004913 0.018434 0.010944 0.004195 0.005905 0.017064 0.010295
Lipid Particles 0.012579 0.004481 0.052571 0.026420 0.002198 0.003362 0.085407 0.033816 0.006295 0.003988 0.064009 0.033139
Mitochondria 0.002663 0.005792 0.001960 0.000086 0.000680 0.004309 0.003113 0.003432 0.001463 0.005138 0.002362 0.003125
Mitotic Spindle 0.000052 0.000802 0.000639 0.001130 0.000047 0.000787 0.000258 0.003728 0.000049 0.000795 0.000507 0.003490
None 0.000090 0.000475 0.000236 0.000422 0.000006 0.000107 0.000095 0.000505 0.000039 0.000313 0.000187 0.000497
Nuclear Periphery 0.000134 0.000146 0.000165 0.000398 0.000059 0.000189 0.000067 0.000142 0.000089 0.000165 0.000131 0.000165
Nuclear Periphery Foci 0.000247 0.000286 0.003352 0.008352 0.000469 0.000529 0.001155 0.003202 0.000381 0.000393 0.002587 0.003673
Nucleolus 0.000543 0.000473 0.000117 0.000145 0.000068 0.000278 0.000053 0.000139 0.000255 0.000387 0.000094 0.000139
Nucleus 0.008502 0.007780 0.048608 0.033718 0.001675 0.009247 0.004237 0.042315 0.004370 0.008426 0.033152 0.041529
Peroxisomes 0.007349 0.004533 0.020076 0.009084 0.001001 0.002007 0.045922 0.020673 0.003506 0.003420 0.029079 0.019612
Vacuole 0.420599* 0.604282* 0.110595 0.399134* 0.450366* 0.698526* 0.042513 0.206688* 0.438618* 0.645824* 0.086879 0.224295*
Vacuole Periphery 0.002871 0.012224 0.000329 0.000164 0.001723 0.006516 0.000746 0.000512 0.002176 0.009708 0.000474 0.000480

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.28 -20.86 -2.31 -3.60 13.16 3.06 -23.68 -10.84 -12.00 16.55* 3.33 -32.06 -10.24 -12.41 20.13*
Bud Neck 6.37 6.43 7.84 8.15 8.04 7.32 7.51 9.21 7.63 7.14 9.87 10.11 11.80 9.10 8.37
Bud Site 2.94 0.55 1.67 -1.14 1.90 2.66 -4.30 -1.78 -8.57 3.99 3.62 -2.24 -0.47 -7.39 2.87
Cell Periphery 5.49 3.80 5.23 -2.05 8.54 12.59 6.06 12.56 -0.69 15.89 13.14 8.75 12.99 -2.54 18.03
Cytoplasm 1.62 -7.00 -2.55 -4.33 2.37 0.05 -6.86 -18.39 -24.96 -14.65 0.68 -11.90 -17.76 -24.84 -9.79
Cytoplasmic Foci -2.85 -4.67 -4.91 -3.73 -2.66 -6.73 -6.30 -19.93 -15.65 -15.96 -7.07 -9.76 -17.64 -14.26 -12.24
Eisosomes 3.31 -7.82 0.92 -2.72 9.88 4.07 -14.98 -2.09 -6.68 14.20 4.93 -17.07 -0.75 -7.15 17.29
Endoplasmic Reticulum 1.63 -14.87 -2.94 -3.65 7.25 3.87 -6.82 1.49 -3.62 8.62 4.36 -13.64 -0.03 -5.26 14.43
Endosome -7.18 3.98 5.11 21.87 4.38 -11.66 -1.64 -0.18 13.36 1.95 -13.96 2.54 3.18 22.66 1.47
Golgi -0.48 -5.05 1.70 2.89 8.59 -1.22 -8.05 -4.13 -2.83 4.80 -1.50 -9.76 -3.30 -2.15 6.12
Lipid Particles 1.81 -7.35 -1.00 -2.67 4.95 -1.47 -16.20 -9.78 -9.16 9.54 1.24 -17.94 -7.16 -9.37 9.37
Mitochondria -2.94 0.95 4.80 6.23 3.10 -3.61 -5.10 -1.64 0.89 0.18 -5.14 -1.80 -0.93 1.85 -0.22
Mitotic Spindle -1.63 -1.62 -1.29 -1.21 -1.18 -1.61 -2.62 -3.59 -2.89 -3.41 -2.28 -1.82 -3.70 -3.13 -3.08
None -1.92 -2.38 -1.38 0.40 -0.63 -3.03 -7.99 -3.58 -2.92 -3.05 -2.48 -4.65 -3.52 -1.46 -2.54
Nuclear Periphery -0.34 -1.63 -4.90 -4.78 -4.53 -3.13 -1.25 -10.96 -0.24 -11.04 -2.96 -3.83 -8.21 -2.54 -5.83
Nuclear Periphery Foci -0.97 -11.23 -6.72 -6.68 -3.69 -0.45 -5.13 -5.17 -4.72 -3.89 -0.33 -11.74 -6.45 -6.31 -2.11
Nucleolus 0.15 1.22 1.08 2.63 -1.19 -3.34 1.63 -3.52 1.38 -3.99 -0.94 1.15 0.48 2.75 -2.86
Nucleus 0.17 -12.92 -5.74 -6.21 1.87 -7.38 -4.79 -16.45 -12.85 -15.32 -4.04 -15.85 -15.69 -14.39 -4.00
Peroxisomes 1.06 -4.45 0.11 -0.84 4.31 -3.36 -14.91 -11.37 -10.69 8.16 0.03 -15.35 -7.98 -9.61 5.98
Vacuole -6.13 10.47 -1.19 4.53 -12.17 -10.60 18.63 8.70 35.82* -22.23 -11.26 19.79 8.84 35.80 -19.92
Vacuole Periphery -12.01 5.44 5.81 19.51 4.24 -8.40 3.41 4.79 11.67 1.80 -15.61 6.93 6.90 21.83 0.57
ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
Localization
Cell Percentages vacuole (36%), cell periphery (7%), mixed (44%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YOR248W

YOR248W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yor248w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available