Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 1 | 1 | 0 | 1 | – | 4 | 5 | 8 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 7 | 3 | 1 | 3 | 4 |
Bud Neck | 0 | 0 | 0 | 2 | 0 | 1 | 2 | – | 1 | 4 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 5 | 4 | – | 5 | 6 | 13 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 20 | 22 | 20 | 14 | 23 | 38 | – | 34 | 26 | 35 | 46 | 29 | 42 | 63 | 15 | 31 | 18 | 1 | 7 | 6 | 7 | 7 | 11 |
Endoplasmic Reticulum | 1 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 13 | 3 | 0 | 0 | 1 | 0 | 0 | 0 |
Endosome | 14 | 28 | 15 | 6 | 6 | 15 | 7 | – | 4 | 5 | 4 | 12 | 13 | 26 | 42 | 17 | 49 | 25 | 14 | 23 | 6 | 0 | 3 | 8 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 4 | 2 | 0 | 1 | 2 | 0 | 1 | 0 |
Mitochondria | 24 | 7 | 0 | 4 | 5 | 38 | 42 | – | 105 | 101 | 177 | 102 | 2 | 2 | 0 | 9 | 10 | 8 | 7 | 4 | 3 | 2 | 8 | 9 |
Nucleus | 225 | 219 | 103 | 49 | 43 | 75 | 75 | – | 117 | 83 | 98 | 80 | 103 | 129 | 98 | 83 | 105 | 84 | 194 | 206 | 98 | 109 | 145 | 180 |
Nuclear Periphery | 3 | 2 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 0 | 0 | 0 |
Nucleolus | 31 | 50 | 12 | 16 | 18 | 31 | 21 | – | 17 | 14 | 11 | 10 | 18 | 36 | 43 | 25 | 27 | 21 | 15 | 19 | 16 | 1 | 6 | 13 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 3 | 0 | – | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 11 | 37 | 14 | 31 | 38 | 49 | 34 | – | 23 | 16 | 14 | 25 | 21 | 50 | 53 | 31 | 34 | 35 | 31 | 65 | 26 | 2 | 5 | 14 |
Vac/Vac Membrane | 16 | 43 | 9 | 5 | 11 | 11 | 10 | – | 13 | 20 | 22 | 25 | 7 | 9 | 11 | 19 | 24 | 27 | 23 | 29 | 24 | 17 | 33 | 58 |
Unique Cell Count | 278 | 334 | 159 | 110 | 112 | 195 | 188 | 250 | 217 | 269 | 240 | 167 | 242 | 250 | 169 | 226 | 173 | 300 | 376 | 205 | 147 | 222 | 310 | |
Labelled Cell Count | 332 | 408 | 177 | 136 | 136 | 251 | 235 | 323 | 281 | 385 | 315 | 195 | 298 | 314 | 206 | 300 | 226 | 300 | 376 | 205 | 147 | 222 | 310 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 6.0 | 6.8 | 6.6 | 6.2 | 5.3 | 5.8 | – | 4.4 | 4.6 | 4.1 | 4.7 | 6.2 | 6.2 | 6.1 | 6.9 | 7.2 | 6.6 | 5.4 | 6.1 | 6.1 |
Std Deviation (1e-4) | 0.8 | 0.9 | 2.1 | 2.8 | 2.2 | 2.0 | 2.9 | – | 1.2 | 1.3 | 1.4 | 1.8 | 1.3 | 1.3 | 1.5 | 1.2 | 1.9 | 1.4 | 1.3 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.13 | -0.37 | -0.22 | – | -0.63 | -0.56 | -0.72 | -0.54 | -0.13 | -0.14 | -0.15 | 0.02 | 0.08 | -0.05 | -0.32 | -0.16 | -0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.0 | -0.3 | -0.6 | 1.6 | – | -0.1 | -0.5 | -0.2 | 1.4 | 0.9 | 0.9 | 2.8 | -1.4 | 0 | -1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 |
Endosome | -1.2 | -1.2 | -0.6 | -2.2 | – | 0 | 0 | 0 | 0 | -0.5 | 0.4 | 2.1 | 0.2 | 3.2 | 1.4 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.9 | 6.4 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -3.3 | -4.3 | -4.9 | -4.6 | – | -3.6 | -5.1 | -5.7 | -6.2 | -0.6 | -2.3 | -5.0 | -2.9 | -3.6 | -3.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.8 | 2.2 | 2.4 | 1.1 | – | 0 | 0 | 0 | 0 | 1.0 | 2.2 | 2.8 | 2.1 | 1.4 | 1.4 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 4.2 | 5.2 | 4.0 | 2.5 | – | 0 | 0 | 0 | 0 | 1.1 | 3.2 | 3.3 | 2.5 | 1.8 | 2.9 |
Vacuole | -0.4 | 1.3 | 0 | -0.1 | – | -0.2 | 1.3 | 1.0 | 1.7 | -0.6 | -0.9 | -0.6 | 1.8 | 1.7 | 2.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.1943 | 0.2273 | -0.4724 | -0.2205 | -0.5838 | -0.2107 | -0.0466 | 0.3747 | 0.3338 | -0.096 | -0.1574 | 0.3368 | 0.4746 | 0.8379 | 0.7415 | 0.8533 | 0.3398 | 0.7438 |
Actin | 0.0204 | 0.0006 | 0.0015 | 0.0051 | 0.0155 | 0.0018 | 0.0061 | 0.0004 | 0.0006 | 0.0114 | 0.0031 | 0.0015 | 0.0005 | 0 | 0.0002 | 0 | 0.0019 | 0.0001 |
Bud | 0.0005 | 0.0001 | 0.001 | 0.0006 | 0.0007 | 0.0001 | 0.0001 | 0.0029 | 0.0001 | 0.0004 | 0.0005 | 0.0001 | 0.0013 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Bud Neck | 0.007 | 0.0018 | 0.0027 | 0.0008 | 0.0024 | 0.0067 | 0.0039 | 0.0009 | 0.0038 | 0.007 | 0.001 | 0.0033 | 0.0004 | 0.0013 | 0.0009 | 0.0009 | 0.0007 | 0.0026 |
Bud Periphery | 0.0007 | 0.0001 | 0.0013 | 0.0015 | 0.0007 | 0.0003 | 0.0001 | 0.0006 | 0.0001 | 0.0005 | 0.0013 | 0.0003 | 0.0003 | 0 | 0 | 0 | 0.0002 | 0 |
Bud Site | 0.0105 | 0.0012 | 0.0084 | 0.0009 | 0.0025 | 0.0003 | 0.0005 | 0.0018 | 0.0063 | 0.0043 | 0.0012 | 0.0002 | 0.0002 | 0.0007 | 0.0004 | 0.0005 | 0.0001 | 0.0001 |
Cell Periphery | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0223 | 0.027 | 0.0193 | 0.0372 | 0.0364 | 0.0286 | 0.0185 | 0.02 | 0.0102 | 0.0075 | 0.0086 | 0.0121 | 0.009 | 0.0122 | 0.0082 | 0.008 | 0.0012 | 0.0079 |
Cytoplasmic Foci | 0.0504 | 0.0112 | 0.0189 | 0.0035 | 0.0151 | 0.0033 | 0.0143 | 0.0029 | 0.0075 | 0.0164 | 0.0068 | 0.0031 | 0.0033 | 0.0012 | 0.0027 | 0.0063 | 0.0013 | 0.0005 |
Eisosomes | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0021 | 0.0018 | 0.0013 | 0.0017 | 0.0019 | 0.0022 | 0.0013 | 0.0009 | 0.0003 | 0.0016 | 0.0009 | 0.0021 | 0.0006 | 0.0004 | 0.0003 | 0.0004 | 0.0005 | 0.0002 |
Endosome | 0.0345 | 0.0076 | 0.0145 | 0.0109 | 0.0168 | 0.01 | 0.011 | 0.0027 | 0.0027 | 0.0197 | 0.0174 | 0.009 | 0.0023 | 0.0016 | 0.0011 | 0.0193 | 0.008 | 0.0005 |
Golgi | 0.0097 | 0.0008 | 0.0049 | 0.0033 | 0.0197 | 0.0022 | 0.0058 | 0.0002 | 0.0008 | 0.0062 | 0.0081 | 0.0031 | 0.0002 | 0.0001 | 0.0002 | 0.0006 | 0.0051 | 0 |
Lipid Particles | 0.0467 | 0.0198 | 0.0055 | 0.0033 | 0.0289 | 0.0042 | 0.0128 | 0.0016 | 0.0025 | 0.0168 | 0.015 | 0.0062 | 0.0015 | 0.0025 | 0.0015 | 0.0011 | 0.0083 | 0.0002 |
Mitochondria | 0.0173 | 0.0004 | 0.003 | 0.0841 | 0.0193 | 0.0079 | 0.0045 | 0.0006 | 0.0005 | 0.0016 | 0.0506 | 0.0025 | 0.0007 | 0.0003 | 0.0001 | 0.0004 | 0.0033 | 0.0001 |
None | 0.028 | 0.1055 | 0.031 | 0.0331 | 0.0154 | 0.0264 | 0.0309 | 0.0363 | 0.0489 | 0.0425 | 0.0628 | 0.0545 | 0.0121 | 0.0046 | 0.0144 | 0.0019 | 0.0003 | 0.0035 |
Nuclear Periphery | 0.0203 | 0.0311 | 0.02 | 0.0323 | 0.0197 | 0.0278 | 0.025 | 0.0282 | 0.0233 | 0.0141 | 0.02 | 0.0213 | 0.0109 | 0.0155 | 0.023 | 0.0111 | 0.0041 | 0.0044 |
Nucleolus | 0.0166 | 0.0556 | 0.0368 | 0.0117 | 0.0207 | 0.0383 | 0.0321 | 0.0497 | 0.0281 | 0.0104 | 0.0096 | 0.0389 | 0.0265 | 0.0616 | 0.0386 | 0.0356 | 0.0285 | 0.0561 |
Nucleus | 0.4076 | 0.401 | 0.3864 | 0.5934 | 0.7042 | 0.6809 | 0.6042 | 0.5217 | 0.4461 | 0.4572 | 0.5751 | 0.6462 | 0.5424 | 0.5778 | 0.4006 | 0.717 | 0.7378 | 0.7072 |
Peroxisomes | 0.0185 | 0.0008 | 0.0013 | 0.007 | 0.0121 | 0.0006 | 0.0096 | 0.0002 | 0.0016 | 0.0096 | 0.0043 | 0.0011 | 0.0015 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0 |
Punctate Nuclear | 0.2766 | 0.331 | 0.4392 | 0.1653 | 0.0651 | 0.1559 | 0.2172 | 0.3271 | 0.4153 | 0.3706 | 0.2086 | 0.1929 | 0.3854 | 0.3179 | 0.5071 | 0.1939 | 0.197 | 0.2162 |
Vacuole | 0.0083 | 0.0016 | 0.0025 | 0.0023 | 0.0018 | 0.0016 | 0.0011 | 0.0009 | 0.0007 | 0.001 | 0.0024 | 0.0009 | 0.0007 | 0.0019 | 0.0003 | 0.0022 | 0.0004 | 0.0002 |
Vacuole Periphery | 0.0014 | 0.0006 | 0.0005 | 0.0019 | 0.0011 | 0.0009 | 0.0005 | 0.0004 | 0.0005 | 0.0005 | 0.0021 | 0.0005 | 0.0003 | 0.0004 | 0.0002 | 0.0006 | 0.0004 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.0935 | 6.71 | 7.4687 | 7.996 | 4.5185 | 3.2151 | 7.5114 | 7.8499 | 7.5122 | 7.7533 |
Translational Efficiency | 0.5329 | 0.4684 | 0.4435 | 0.4639 | 0.6482 | 0.7789 | 0.4834 | 0.4091 | 0.3937 | 0.4464 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2014 | 1181 | 213 | 1618 | 1901 | 1460 | 2115 | 525 | 3915 | 2641 | 2328 | 2143 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 670.10 | 754.25 | 916.50 | 902.09 | 686.47 | 749.92 | 902.14 | 919.30 | 678.05 | 751.86 | 903.45 | 906.31 |
Standard Deviation | 75.45 | 110.08 | 111.36 | 127.03 | 82.48 | 101.19 | 108.69 | 124.24 | 79.37 | 105.28 | 109.01 | 126.57 |
Intensity Change Log 2 | — | 0.170666 | 0.451758 | 0.428895 | — | 0.127540 | 0.394155 | 0.421339 | — | 0.149004 | 0.422896 | 0.425076 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000199 | 0.000796 | 0.001771 | 0.001899 | 0.000255 | 0.000445 | 0.000533 | 0.003186 | 0.000226 | 0.000602 | 0.000646 | 0.002214 |
Bud Neck | 0.005400 | 0.005952 | 0.007968 | 0.028480 | 0.005517 | 0.007732 | 0.011809 | 0.021421 | 0.005457 | 0.006936 | 0.011458 | 0.026751 |
Bud Site | 0.001995 | 0.002645 | 0.002486 | 0.027050 | 0.001677 | 0.004014 | 0.002929 | 0.021639 | 0.001841 | 0.003402 | 0.002888 | 0.025724 |
Cell Periphery | 0.000128 | 0.000150 | 0.000109 | 0.000228 | 0.000116 | 0.000120 | 0.000118 | 0.000202 | 0.000122 | 0.000133 | 0.000118 | 0.000222 |
Cytoplasm | 0.083631 | 0.055214 | 0.018472 | 0.065255 | 0.079080 | 0.056406 | 0.034541 | 0.024139 | 0.081421 | 0.055873 | 0.033070 | 0.055183 |
Cytoplasmic Foci | 0.013239 | 0.007257 | 0.000665 | 0.010645 | 0.012165 | 0.011349 | 0.001669 | 0.012696 | 0.012717 | 0.009519 | 0.001577 | 0.011148 |
Eisosomes | 0.000031 | 0.000040 | 0.000045 | 0.000039 | 0.000032 | 0.000037 | 0.000027 | 0.000040 | 0.000031 | 0.000039 | 0.000028 | 0.000039 |
Endoplasmic Reticulum | 0.000975 | 0.005007 | 0.009652 | 0.006572 | 0.001817 | 0.003791 | 0.004005 | 0.005077 | 0.001384 | 0.004335 | 0.004522 | 0.006206 |
Endosome | 0.001342 | 0.004262 | 0.000746 | 0.010488 | 0.001158 | 0.003007 | 0.001144 | 0.018799 | 0.001253 | 0.003568 | 0.001107 | 0.012524 |
Golgi | 0.000643 | 0.001923 | 0.000251 | 0.009274 | 0.000645 | 0.001395 | 0.000300 | 0.014940 | 0.000644 | 0.001631 | 0.000296 | 0.010662 |
Lipid Particles | 0.002668 | 0.002593 | 0.000680 | 0.002653 | 0.002378 | 0.003226 | 0.000602 | 0.003588 | 0.002527 | 0.002943 | 0.000610 | 0.002882 |
Mitochondria | 0.002693 | 0.006097 | 0.001245 | 0.011698 | 0.004534 | 0.007927 | 0.002334 | 0.023343 | 0.003587 | 0.007109 | 0.002234 | 0.014551 |
Mitotic Spindle | 0.003167 | 0.018397 | 0.064510 | 0.301038* | 0.005845 | 0.019163 | 0.087918 | 0.405421* | 0.004467 | 0.018821 | 0.085776 | 0.326610* |
None | 0.026571 | 0.017447 | 0.003442 | 0.010246 | 0.020916 | 0.010161 | 0.010053 | 0.013070 | 0.023825 | 0.013419 | 0.009448 | 0.010938 |
Nuclear Periphery | 0.001375 | 0.002738 | 0.001363 | 0.009011 | 0.001874 | 0.003265 | 0.002071 | 0.005926 | 0.001617 | 0.003030 | 0.002006 | 0.008255 |
Nuclear Periphery Foci | 0.009854 | 0.015536 | 0.017504 | 0.018950 | 0.013982 | 0.006917 | 0.011021 | 0.028332 | 0.011859 | 0.010771 | 0.011614 | 0.021248 |
Nucleolus | 0.071736 | 0.044536 | 0.024357 | 0.012084 | 0.067681 | 0.040645 | 0.023894 | 0.007224 | 0.069767 | 0.042385 | 0.023936 | 0.010894 |
Nucleus | 0.771015* | 0.800319* | 0.838395* | 0.432005* | 0.776753* | 0.809213* | 0.800180* | 0.369553* | 0.773801* | 0.805236* | 0.803677* | 0.416705* |
Peroxisomes | 0.001974 | 0.002031 | 0.001004 | 0.003534 | 0.002066 | 0.003817 | 0.000624 | 0.001255 | 0.002019 | 0.003018 | 0.000659 | 0.002975 |
Vacuole | 0.001113 | 0.003966 | 0.005115 | 0.028980 | 0.001289 | 0.006051 | 0.003871 | 0.017579 | 0.001199 | 0.005118 | 0.003984 | 0.026187 |
Vacuole Periphery | 0.000250 | 0.003093 | 0.000221 | 0.009871 | 0.000220 | 0.001320 | 0.000358 | 0.002573 | 0.000236 | 0.002113 | 0.000345 | 0.008083 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -7.19 | -9.87 | -7.45 | -3.51 | 1.65 | -4.19 | -14.04 | -5.56 | -4.83 | -3.54 | -8.25 | -16.64 | -9.18 | -6.26 | -4.15 |
Bud Neck | -1.54 | -4.44 | -14.41 | -13.80 | -3.69 | -3.10 | -12.03 | -8.97 | -7.66 | -3.06 | -3.39 | -13.28 | -16.94 | -15.36 | -6.88 |
Bud Site | -1.93 | -3.63 | -11.22 | -10.45 | -6.30 | -2.58 | -7.70 | -6.92 | -5.53 | -4.50 | -3.17 | -8.58 | -13.23 | -11.35 | -9.08 |
Cell Periphery | -1.24 | -0.16 | -2.06 | -0.81 | -1.50 | 0.14 | 0.65 | -2.70 | -2.55 | -3.19 | -0.78 | 0.73 | -3.39 | -2.64 | -4.82 |
Cytoplasm | 4.63 | 8.34 | 6.42 | 1.17 | -4.61 | 3.90 | 13.11 | 12.25 | 8.30 | 0.43 | 6.02 | 17.90 | 10.28 | 4.01 | -7.14 |
Cytoplasmic Foci | 7.54 | 12.30 | 10.93 | 3.56 | -4.05 | 2.14 | 10.70 | 6.48 | 4.56 | -2.23 | 7.13 | 15.85 | 13.38 | 6.79 | -4.11 |
Eisosomes | -1.54 | -2.49 | -4.32 | -2.03 | 0.44 | -1.00 | -2.22 | -4.22 | -3.43 | -2.92 | -1.74 | -2.69 | -6.11 | -3.99 | -3.17 |
Endoplasmic Reticulum | -13.22 | -7.03 | -16.27 | -7.01 | 0.90 | -6.02 | -15.18 | -11.42 | -7.59 | -6.33 | -12.74 | -18.58 | -19.07 | -10.22 | -7.49 |
Endosome | -3.27 | 1.42 | -6.16 | -0.81 | -7.03 | -4.01 | -0.53 | -4.31 | -3.06 | -4.25 | -4.89 | -0.52 | -7.57 | -2.96 | -7.46 |
Golgi | -1.74 | 2.60 | -2.56 | -1.19 | -3.25 | -1.02 | 1.60 | -1.99 | -1.65 | -2.18 | -1.92 | 2.43 | -3.26 | -2.12 | -3.76 |
Lipid Particles | 2.09 | 8.04 | 4.17 | 2.46 | -2.15 | -1.45 | 8.63 | 1.64 | 2.66 | -3.53 | 0.14 | 12.93 | 4.32 | 4.01 | -3.98 |
Mitochondria | -2.50 | 2.25 | -5.12 | -0.70 | -8.62 | -2.36 | 2.01 | -1.42 | -0.22 | -2.16 | -3.53 | 2.07 | -3.76 | -0.17 | -5.67 |
Mitotic Spindle | -6.14 | -5.46 | -29.16* | -24.45* | -8.21 | -3.93 | -20.89* | -19.39 | -18.40 | -10.92 | -7.22 | -22.09 | -34.81 | -31.33* | -14.98 |
None | 6.88 | 17.02 | 13.14 | 5.64 | -5.65 | 7.33 | 9.05 | 6.35 | 0.91 | 0.04 | 10.42 | 15.71 | 14.57 | 4.57 | -0.45 |
Nuclear Periphery | -3.71 | -1.68 | -9.72 | -7.29 | -8.65 | -5.21 | -6.31 | -6.92 | -4.91 | -5.20 | -6.41 | -8.65 | -11.13 | -8.46 | -8.70 |
Nuclear Periphery Foci | -1.66 | 0.63 | 1.67 | 3.02 | 0.13 | 4.49 | 2.82 | -0.18 | -3.26 | -2.09 | 1.85 | 2.46 | 1.98 | 0.08 | -0.45 |
Nucleolus | 9.20 | 6.07 | 15.97 | 5.71 | 2.39 | 6.41 | 3.88 | 14.45 | 7.90 | 12.01 | 11.07 | 7.11 | 20.61 | 8.72 | 11.61 |
Nucleus | -5.56 | -2.08 | 16.51 | 20.31 | 9.87 | -5.11 | 4.10 | 14.34 | 17.22 | 11.79 | -7.75 | 2.63 | 22.72 | 28.08 | 17.70 |
Peroxisomes | 0.07 | 1.57 | 1.85 | 1.53 | 0.23 | -1.96 | 2.82 | 2.82 | 4.17 | 0.08 | -1.69 | 4.39 | 2.47 | 3.58 | -1.13 |
Vacuole | -5.25 | -4.04 | -13.70 | -12.28 | -8.66 | -5.65 | -7.12 | -8.56 | -6.93 | -7.20 | -7.39 | -8.31 | -16.04 | -13.51 | -13.07 |
Vacuole Periphery | -3.08 | -1.24 | -3.73 | -0.34 | -3.63 | -3.57 | -1.30 | -3.00 | 0.68 | -2.65 | -4.24 | -1.19 | -4.04 | -0.97 | -3.91 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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