Standard name | |
---|---|
Human Ortholog | |
Description | Essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec62p, Sec66p, and Sec72p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 20 | 26 | 29 | 47 | 50 | 48 | 64 | 62 | 50 | 57 | 58 | 54 | 43 | 45 | 26 | – | 2 | 3 | 1 | 5 | 8 | 5 | 13 | 13 |
Bud | 0 | 10 | 1 | 2 | 2 | 1 | 6 | 5 | 3 | 3 | 7 | 4 | 1 | 1 | 2 | – | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 17 | 47 | 29 | 44 | 34 | 48 | 55 | 46 | 60 | 46 | 59 | 82 | 82 | 108 | – | 14 | 22 | 2 | 4 | 15 | 6 | 25 | 34 |
Cytoplasm | 3 | 33 | 12 | 16 | 25 | 28 | 29 | 50 | 37 | 42 | 45 | 46 | 15 | 15 | 46 | – | 21 | 8 | 0 | 7 | 0 | 3 | 4 | 3 |
Endoplasmic Reticulum | 184 | 221 | 304 | 246 | 335 | 386 | 549 | 726 | 355 | 426 | 295 | 376 | 423 | 429 | 483 | – | 1 | 2 | 192 | 256 | 292 | 377 | 515 | 571 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 13 | 2 | 27 | 68 | 100 | 21 | 18 | 22 | 10 | 10 | 5 | 6 | 5 | 5 | 1 | – | 0 | 5 | 1 | 2 | 1 | 2 | 7 | 5 |
Mitochondria | 4 | 14 | 12 | 34 | 30 | 77 | 86 | 89 | 96 | 126 | 138 | 165 | 0 | 0 | 0 | – | 4 | 5 | 0 | 3 | 1 | 6 | 6 | 7 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 3 | 5 | 4 | 9 | 39 | 34 | 28 | 21 | 13 | 22 | 2 | 2 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 4 | 5 | 2 | 2 | 23 | 10 | 25 | 35 | 7 | 20 | 10 | 19 | 0 | 2 | 14 | – | 1 | 2 | 0 | 5 | 0 | 0 | 2 | 5 |
Unique Cell Count | 195 | 276 | 336 | 297 | 439 | 469 | 659 | 868 | 471 | 582 | 435 | 560 | 467 | 472 | 569 | 39 | 41 | 200 | 291 | 324 | 406 | 581 | 649 | |
Labelled Cell Count | 237 | 328 | 438 | 449 | 613 | 615 | 866 | 1084 | 632 | 769 | 619 | 752 | 572 | 582 | 680 | 45 | 48 | 200 | 291 | 324 | 406 | 581 | 649 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 20.3 | 15.9 | 21.3 | 21.2 | 18.9 | 15.5 | 14.8 | 14.6 | 14.0 | 13.1 | 12.8 | 13.1 | 19.4 | 19.4 | 19.6 | – | 14.9 | 18.5 | 23.2 | 23.2 | 23.4 |
Std Deviation (1e-4) | 3.3 | 3.1 | 3.9 | 4.2 | 3.3 | 2.9 | 2.7 | 2.7 | 2.6 | 2.6 | 2.5 | 2.8 | 2.8 | 2.8 | 3.3 | – | 4.1 | 4.1 | 3.7 | 4.0 | 4.1 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.17 | -0.46 | -0.53 | -0.54 | -0.61 | -0.7 | -0.74 | -0.71 | -0.14 | -0.14 | -0.12 | – | -0.52 | -0.2 | 0.12 | 0.12 | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 2.8 | 1.3 | 0.8 | 0.6 | -0.9 | 0.9 | 0.6 | 2.0 | 0.5 | 0.3 | 0.4 | -2.5 | – | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | -1.6 | -1.7 | -3.1 | -3.4 | -4.3 | -1.9 | -1.7 | -1.4 | -1.5 | 1.4 | 1.3 | 1.9 | – | 3.5 | 6.2 |
Cytoplasm | 1.1 | 1.4 | 1.5 | 0.6 | 1.5 | 2.5 | 2.3 | 3.6 | 2.7 | -0.3 | -0.3 | 2.7 | – | 0 | 0 |
Endoplasmic Reticulum | -2.8 | -5.1 | -3.3 | -3.1 | -3.0 | -5.5 | -6.2 | -7.5 | -7.9 | 0 | 0.2 | -2.4 | – | -13.3 | -13.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Golgi | 5.2 | 5.5 | -2.1 | -3.8 | -4.3 | -4.0 | -4.7 | -4.8 | -5.4 | -5.0 | -5.0 | -6.6 | – | 0 | 0 |
Mitochondria | 3.8 | 2.0 | 5.7 | 4.7 | 3.8 | 6.9 | 7.4 | 9.8 | 9.4 | -4.1 | 0 | 0 | – | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1.2 | 3.7 | 2.7 | 3.7 | 2.5 | 2.0 | 2.7 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Vacuole | 0 | 3.6 | 1.8 | 2.9 | 3.1 | 0 | 2.7 | 1.9 | 2.7 | 0 | 0 | 2.1 | – | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 20.419 | 23.0787 | 22.0012 | 19.0344 | 17.223 | 21.8847 | 19.9389 | 24.7852 | 23.6173 | 22.4001 | 20.197 | 21.7537 | 22.219 | 25.2868 | 22.8653 | 22.4332 | 21.4088 | 23.3429 |
Actin | 0.0164 | 0.0006 | 0.0195 | 0.0367 | 0.0665 | 0.004 | 0.0844 | 0.0003 | 0.0163 | 0.0001 | 0.1502 | 0.0027 | 0.014 | 0.0047 | 0.0075 | 0 | 0.0015 | 0.0034 |
Bud | 0.0004 | 0.0001 | 0.0003 | 0.0006 | 0.001 | 0.0001 | 0.0005 | 0 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 |
Bud Neck | 0.0029 | 0.0005 | 0.0008 | 0.0002 | 0.0018 | 0.0016 | 0.002 | 0.0003 | 0.001 | 0.0002 | 0.0018 | 0.0015 | 0.001 | 0.0011 | 0.0011 | 0.0001 | 0.0036 | 0.0013 |
Bud Periphery | 0.0007 | 0.0003 | 0.0011 | 0.0014 | 0.0014 | 0.0001 | 0.0007 | 0.0002 | 0.0005 | 0.001 | 0.001 | 0.0004 | 0.0005 | 0.0005 | 0.0011 | 0 | 0.0006 | 0.0001 |
Bud Site | 0.0117 | 0.0002 | 0.0021 | 0.0006 | 0.0084 | 0.0001 | 0.0016 | 0.0001 | 0.0127 | 0 | 0.001 | 0.0016 | 0.0019 | 0.0059 | 0.0079 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0032 | 0.0147 | 0.0101 | 0.0049 | 0.006 | 0.0009 | 0.0064 | 0.0141 | 0.0128 | 0.0047 | 0.0049 | 0.002 | 0.0079 | 0.0267 | 0.013 | 0.007 | 0.0071 | 0.0037 |
Cytoplasm | 0.0089 | 0.0021 | 0.0037 | 0.0023 | 0.0055 | 0.0014 | 0.0055 | 0.0019 | 0.0072 | 0.0005 | 0.001 | 0.0011 | 0.0073 | 0.0081 | 0.0072 | 0.0008 | 0.0409 | 0.0026 |
Cytoplasmic Foci | 0.0113 | 0.0006 | 0.0025 | 0.004 | 0.0151 | 0.0012 | 0.0094 | 0.0003 | 0.0091 | 0 | 0.0014 | 0.0058 | 0.0112 | 0.0006 | 0.0068 | 0 | 0.0021 | 0.0006 |
Eisosomes | 0.0003 | 0.0007 | 0.0004 | 0.0007 | 0.0007 | 0 | 0.001 | 0.0015 | 0.0002 | 0.0001 | 0.0011 | 0.0001 | 0.0006 | 0.001 | 0.0016 | 0.0001 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.8232 | 0.9435 | 0.9119 | 0.8762 | 0.7317 | 0.9669 | 0.7209 | 0.946 | 0.8927 | 0.9775 | 0.8007 | 0.9642 | 0.8462 | 0.9156 | 0.8879 | 0.9845 | 0.851 | 0.9622 |
Endosome | 0.0248 | 0.0085 | 0.0119 | 0.0116 | 0.0284 | 0.0067 | 0.0353 | 0.0021 | 0.0059 | 0.0007 | 0.007 | 0.0027 | 0.0151 | 0.0009 | 0.0091 | 0.0004 | 0.0044 | 0.0011 |
Golgi | 0.0089 | 0.0025 | 0.0064 | 0.0051 | 0.016 | 0.0074 | 0.0122 | 0.0025 | 0.0067 | 0.0006 | 0.0135 | 0.0054 | 0.0102 | 0.0036 | 0.0048 | 0.0011 | 0.0046 | 0.0043 |
Lipid Particles | 0.0134 | 0.0087 | 0.0059 | 0.0054 | 0.0163 | 0.0023 | 0.0195 | 0.0149 | 0.0057 | 0.0001 | 0.0018 | 0.0026 | 0.0068 | 0.0184 | 0.0149 | 0.0003 | 0.0361 | 0.004 |
Mitochondria | 0.0054 | 0.0005 | 0.0058 | 0.0307 | 0.052 | 0.0007 | 0.0034 | 0.0002 | 0.003 | 0.0005 | 0.006 | 0.0006 | 0.0015 | 0.0002 | 0.0041 | 0 | 0.0069 | 0.0002 |
None | 0.0071 | 0.0001 | 0.0001 | 0.0029 | 0.0012 | 0 | 0.0049 | 0.0001 | 0.0033 | 0 | 0.0003 | 0.0001 | 0.0248 | 0.0001 | 0.0132 | 0 | 0.0003 | 0 |
Nuclear Periphery | 0.0356 | 0.0051 | 0.0077 | 0.006 | 0.0046 | 0.0042 | 0.069 | 0.0079 | 0.0124 | 0.0079 | 0.0046 | 0.0049 | 0.0243 | 0.0093 | 0.0104 | 0.0038 | 0.0206 | 0.0065 |
Nucleolus | 0.0017 | 0.0001 | 0 | 0.0001 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Nucleus | 0.003 | 0.0002 | 0.0003 | 0.0004 | 0.0009 | 0.0002 | 0.0029 | 0.0002 | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.0021 | 0.0005 | 0.0005 | 0.0001 | 0.001 | 0.0002 |
Peroxisomes | 0.0046 | 0 | 0.0006 | 0.0012 | 0.0116 | 0.0003 | 0.0026 | 0 | 0.0036 | 0 | 0.0006 | 0.0016 | 0.0084 | 0 | 0.0011 | 0 | 0.0005 | 0.0077 |
Punctate Nuclear | 0.0032 | 0 | 0.0001 | 0.0013 | 0.0034 | 0 | 0.005 | 0 | 0.0017 | 0 | 0.0002 | 0.0002 | 0.0108 | 0.0001 | 0.0001 | 0 | 0.0005 | 0 |
Vacuole | 0.0091 | 0.0089 | 0.0072 | 0.0059 | 0.017 | 0.0014 | 0.0079 | 0.0068 | 0.0034 | 0.0042 | 0.0014 | 0.0019 | 0.0031 | 0.0025 | 0.0057 | 0.0012 | 0.0043 | 0.0012 |
Vacuole Periphery | 0.0041 | 0.0021 | 0.0017 | 0.002 | 0.01 | 0.0004 | 0.0046 | 0.0005 | 0.0006 | 0.0016 | 0.0009 | 0.0004 | 0.0013 | 0.0003 | 0.0015 | 0.0003 | 0.0134 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 112.7223 | 80.7125 | 84.5104 | 90.696 | 91.2087 | 92.4531 | 74.3487 | 82.6202 | 79.1986 | 109.2559 |
Translational Efficiency | 1.2973 | 1.0563 | 1.0647 | 1.0427 | 1.1223 | 1.2564 | 1.0158 | 0.8601 | 1.078 | 0.9839 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
156 | 594 | 263 | 873 | 1852 | 1174 | 490 | 1436 | 2008 | 1768 | 753 | 2309 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1685.23 | 1765.38 | 2242.64 | 2068.09 | 1586.12 | 1735.72 | 2046.07 | 1882.40 | 1593.82 | 1745.68 | 2114.73 | 1952.61 |
Standard Deviation | 245.22 | 373.74 | 304.71 | 332.16 | 346.05 | 305.76 | 296.52 | 327.41 | 340.32 | 330.46 | 313.73 | 341.31 |
Intensity Change Log 2 | — | 0.067033 | 0.412253 | 0.295353 | — | 0.130032 | 0.367354 | 0.247071 | — | 0.097922 | 0.390658 | 0.272145 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002035 | 0.000995 | 0.000463 | 0.002163 | 0.000919 | 0.001483 | 0.000133 | 0.004111 | 0.001006 | 0.001319 | 0.000248 | 0.003375 |
Bud Neck | 0.003129 | 0.000366 | 0.002956 | 0.000760 | 0.000172 | 0.000357 | 0.001412 | 0.003967 | 0.000402 | 0.000360 | 0.001951 | 0.002754 |
Bud Site | 0.012332 | 0.002698 | 0.002728 | 0.003312 | 0.001939 | 0.003491 | 0.000942 | 0.023958 | 0.002747 | 0.003225 | 0.001566 | 0.016152 |
Cell Periphery | 0.038616 | 0.007857 | 0.022486 | 0.059225 | 0.007779 | 0.008584 | 0.022548 | 0.027690 | 0.010174 | 0.008340 | 0.022527 | 0.039613 |
Cytoplasm | 0.001306 | 0.001675 | 0.000058 | 0.001229 | 0.001637 | 0.001387 | 0.000544 | 0.014959 | 0.001611 | 0.001484 | 0.000374 | 0.009768 |
Cytoplasmic Foci | 0.009883 | 0.001813 | 0.000042 | 0.000665 | 0.004969 | 0.001549 | 0.000322 | 0.010532 | 0.005351 | 0.001638 | 0.000224 | 0.006802 |
Eisosomes | 0.000403 | 0.000741 | 0.000627 | 0.000876 | 0.000174 | 0.000379 | 0.000602 | 0.000496 | 0.000192 | 0.000501 | 0.000611 | 0.000640 |
Endoplasmic Reticulum | 0.793409* | 0.950842* | 0.941614* | 0.857551* | 0.949770* | 0.964223* | 0.948419* | 0.767658* | 0.937622* | 0.959727* | 0.946042* | 0.801645* |
Endosome | 0.013129 | 0.007872 | 0.005044 | 0.004779 | 0.007499 | 0.004413 | 0.003350 | 0.020772 | 0.007936 | 0.005575 | 0.003942 | 0.014725 |
Golgi | 0.024527 | 0.007083 | 0.004454 | 0.011978 | 0.009906 | 0.002856 | 0.004743 | 0.020309 | 0.011042 | 0.004276 | 0.004642 | 0.017159 |
Lipid Particles | 0.053220 | 0.007318 | 0.002763 | 0.031833 | 0.005821 | 0.006189 | 0.007812 | 0.070650 | 0.009504 | 0.006568 | 0.006049 | 0.055974 |
Mitochondria | 0.002232 | 0.000332 | 0.000227 | 0.000283 | 0.000946 | 0.000511 | 0.000104 | 0.001355 | 0.001046 | 0.000451 | 0.000147 | 0.000950 |
Mitotic Spindle | 0.001080 | 0.000665 | 0.000080 | 0.000465 | 0.000098 | 0.000085 | 0.000071 | 0.003073 | 0.000174 | 0.000280 | 0.000074 | 0.002087 |
None | 0.000020 | 0.000452 | 0.000012 | 0.000086 | 0.001159 | 0.000107 | 0.000162 | 0.000730 | 0.001071 | 0.000223 | 0.000110 | 0.000486 |
Nuclear Periphery | 0.001402 | 0.000445 | 0.001949 | 0.004155 | 0.000380 | 0.000250 | 0.000915 | 0.001204 | 0.000459 | 0.000315 | 0.001276 | 0.002320 |
Nuclear Periphery Foci | 0.012314 | 0.001959 | 0.002689 | 0.002016 | 0.001433 | 0.000633 | 0.001383 | 0.008635 | 0.002278 | 0.001079 | 0.001839 | 0.006133 |
Nucleolus | 0.000011 | 0.000010 | 0.000007 | 0.000013 | 0.000009 | 0.000004 | 0.000004 | 0.000028 | 0.000009 | 0.000006 | 0.000005 | 0.000022 |
Nucleus | 0.000304 | 0.000097 | 0.000063 | 0.000116 | 0.000077 | 0.000042 | 0.000036 | 0.000568 | 0.000094 | 0.000060 | 0.000045 | 0.000397 |
Peroxisomes | 0.000604 | 0.000125 | 0.000010 | 0.000266 | 0.000360 | 0.000654 | 0.000105 | 0.001349 | 0.000379 | 0.000476 | 0.000072 | 0.000939 |
Vacuole | 0.023525 | 0.004166 | 0.009814 | 0.014336 | 0.002553 | 0.001536 | 0.005283 | 0.014500 | 0.004183 | 0.002420 | 0.006866 | 0.014438 |
Vacuole Periphery | 0.006516 | 0.002488 | 0.001915 | 0.003894 | 0.002400 | 0.001267 | 0.001110 | 0.003456 | 0.002720 | 0.001677 | 0.001391 | 0.003621 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.17 | 1.97 | -0.08 | -0.97 | -1.48 | -0.81 | 2.27 | -5.11 | -3.30 | -7.41 | -0.56 | 2.32 | -3.79 | -2.98 | -5.66 |
Bud Neck | 3.11 | 0.52 | 2.72 | -2.40 | 1.63 | -2.17 | -1.27 | -8.65 | -8.17 | -2.64 | 0.97 | -1.89 | -7.85 | -8.45 | -1.15 |
Bud Site | 2.46 | 2.33 | 2.30 | -0.67 | -0.34 | -1.50 | 1.68 | -9.48 | -8.26 | -9.78 | -0.22 | 1.60 | -8.44 | -7.98 | -8.92 |
Cell Periphery | 4.52 | 1.89 | -2.66 | -11.47 | -5.52 | -0.61 | -4.24 | -8.90 | -8.40 | -1.28 | 1.43 | -4.15 | -12.75 | -14.03 | -4.85 |
Cytoplasm | -0.04 | 3.08 | 0.48 | 0.46 | -1.62 | 0.34 | 3.40 | -8.18 | -7.75 | -8.82 | 0.18 | 4.90 | -7.61 | -7.05 | -8.83 |
Cytoplasmic Foci | 2.09 | 2.56 | 2.41 | 1.38 | -3.20 | 3.87 | 6.66 | -4.40 | -7.42 | -9.39 | 4.46 | 7.26 | -1.47 | -6.31 | -9.56 |
Eisosomes | -0.67 | -0.79 | -1.64 | -0.25 | -0.71 | -1.63 | -1.98 | -3.44 | -0.76 | 0.47 | -1.67 | -2.52 | -3.91 | -0.65 | -0.14 |
Endoplasmic Reticulum | -6.13 | -5.67 | -2.64 | 9.52 | 7.35 | -3.03 | 0.21 | 20.76 | 21.98 | 18.06 | -4.70 | -1.48 | 19.39 | 23.55 | 18.99 |
Endosome | 1.96 | 3.06 | 3.26 | 3.07 | 0.31 | 4.32 | 5.92 | -10.12 | -12.79 | -13.68 | 3.35 | 5.93 | -7.25 | -10.32 | -12.44 |
Golgi | 1.91 | 2.22 | 1.35 | -3.05 | -5.29 | 5.46 | 3.87 | -4.66 | -9.01 | -7.93 | 4.88 | 4.64 | -3.37 | -9.56 | -9.35 |
Lipid Particles | 4.20 | 4.63 | 1.93 | -6.59 | -8.26 | -0.26 | -0.87 | -15.87 | -15.28 | -13.49 | 2.07 | 1.83 | -15.20 | -16.44 | -15.40 |
Mitochondria | 1.25 | 1.31 | 1.28 | 0.27 | -0.34 | 1.23 | 2.71 | -0.92 | -2.31 | -3.90 | 1.79 | 2.86 | 0.25 | -2.11 | -3.83 |
Mitotic Spindle | 0.97 | 2.32 | 1.60 | 0.47 | -4.86 | 0.31 | 1.24 | -3.81 | -3.82 | -3.84 | -0.58 | 2.52 | -3.87 | -3.60 | -4.09 |
None | -1.35 | 1.30 | -2.88 | 1.17 | -5.26 | 3.44 | 3.16 | 1.16 | -2.98 | -2.56 | 2.81 | 3.36 | 1.88 | -1.57 | -2.71 |
Nuclear Periphery | 2.70 | -1.07 | -5.22 | -9.67 | -4.48 | 3.80 | -5.97 | -8.93 | -10.37 | -2.42 | 2.92 | -6.26 | -11.75 | -12.72 | -5.26 |
Nuclear Periphery Foci | 1.76 | 1.66 | 1.78 | 0.10 | 0.95 | 2.71 | 0.06 | -6.96 | -7.85 | -6.65 | 2.00 | 0.66 | -4.83 | -7.31 | -5.90 |
Nucleolus | 0.83 | 1.10 | 0.71 | -0.41 | -1.57 | 2.23 | 2.57 | -3.11 | -3.90 | -3.94 | 1.31 | 2.16 | -2.93 | -3.67 | -4.28 |
Nucleus | 1.20 | 1.26 | 1.14 | -0.71 | -1.86 | 2.25 | 2.49 | -5.17 | -5.53 | -5.58 | 1.42 | 1.76 | -3.70 | -5.56 | -5.86 |
Peroxisomes | 1.12 | 1.40 | 0.76 | -1.25 | -2.44 | -1.47 | 3.86 | -3.08 | -1.84 | -3.88 | -0.70 | 4.93 | -2.68 | -1.91 | -4.27 |
Vacuole | 3.21 | 2.34 | 1.65 | -5.59 | -2.11 | 1.87 | -2.93 | -9.08 | -9.78 | -6.43 | 2.39 | -2.43 | -9.07 | -11.39 | -6.44 |
Vacuole Periphery | 1.12 | 1.31 | 0.70 | -0.98 | -1.56 | 1.71 | 2.11 | -1.39 | -2.56 | -2.87 | 1.58 | 2.25 | -1.27 | -2.57 | -3.20 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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