Standard name
Human Ortholog
Description ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0.09 0 – – 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 – 0 0.07 0.14 0 0.13 0.05 0.05 0.05 0.09 0 – – 0.37 0.17 0.12 0.19 0 0 0 0 0 0.11
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 – – 0.05 0 0 0.06 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0 0 0.05 0 0 0.07 – – 0 0 0 0 0 0 0 0 0 0
Nucleus 0.75 – 0.9 0.78 0.81 0.83 0.65 0.72 0.59 0.69 0.65 0.56 – – 0.53 0.71 0.71 0.59 0.75 0.91 0.81 0.72 0.83 0.71
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 – 0 0.15 0.06 0.13 0.09 0.07 0.2 0.18 0.17 0.33 – – 0 0 0.06 0.05 0.1 0 0.06 0 0.07 0.09
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.19 – 0.06 0 0 0.05 0.15 0.21 0.17 0.11 0 0.13 – – 0.05 0.08 0.1 0.17 0.05 0 0.06 0.28 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 1 0 0 0 0 0 0 1 0 – – 0 1 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 2 1 0 2 0 0 – – 0 0 0 0 0 0 3 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 1 1 0 2 0 – – 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 1 0 1 0 0 0 – – 0 1 0 0 0 0 0 0 0 0
Cytoplasm 2 – 2 2 5 3 22 9 4 6 2 2 – – 7 45 39 31 1 0 0 0 0 3
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 – – 0 5 9 4 0 0 1 0 0 0
Endosome 0 – 0 0 0 0 0 0 1 0 0 0 – – 0 1 4 1 1 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 1 0 0 0 0 – – 1 3 5 10 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 1 1 0 4 3 1 4 – – 0 8 2 2 0 0 0 0 0 0
Nucleus 12 – 47 21 29 64 106 118 44 90 15 30 – – 10 186 232 96 30 13 69 1 20 24
Nuclear Periphery 0 – 0 0 0 0 0 1 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Nucleolus 2 – 2 4 2 10 14 11 15 23 4 18 – – 0 5 21 8 3 0 5 0 1 3
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 3 – 3 0 1 4 24 34 13 15 1 7 – – 1 22 32 27 1 0 5 0 1 1
Unique Cell Count 16 52 27 36 77 163 164 75 131 23 54 19 261 325 162 40 15 85 2 25 35
Labelled Cell Count 19 54 28 37 82 170 176 83 139 26 61 19 277 344 179 40 15 85 2 25 35


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.3 – 27.4 25.4 26.4 23.1 23.4 24.1 21.7 21.8 21.9 19.1 – – 26.1 33.5 32.9 33.8 – – –
Std Deviation (1e-4) 10.3 – 7.8 6.1 7.5 5.7 7.2 7.2 6.4 6.7 7.6 6.6 – – 11.9 15.1 14.0 15.8 – – –
Intensity Change (Log2) – – – -0.11 -0.05 -0.24 -0.23 -0.18 -0.34 -0.33 -0.32 -0.52 – – -0.07 0.29 0.27 0.3 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Cytoplasm 0 0 0 1.9 0 0 0 0 0 – – 0 2.5 1.8 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Nucleus -1.5 -1.3 -1.2 -3.5 -2.7 -3.9 -3.0 -2.7 -4.0 – – -3.5 -2.9 -2.9 -4.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Nucleolus 0 0 0 0 0 2.6 2.4 0 3.9 – – 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Vacuole 0 0 0 1.7 2.5 1.9 1.2 0 0 – – 0 0 0 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – – – – –
Bud Periphery – – – – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – – – – –
Cytoplasmic Foci – – – – – – – – – – – – – – – – – –
Eisosomes – – – – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – – – – –
Lipid Particles – – – – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – – – – –
None – – – – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – – – – –
Punctate Nuclear – – – – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – – – – –
Vacuole Periphery – – – – – – – – – – – – – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 91.9504 86.3267 97.4798 115.8793 91.4547 88.1228 102.4648 122.1771 125.8127 96.8333
Translational Efficiency 2.257 1.9746 2.0216 1.7808 1.8324 2.4987 1.9521 1.559 1.5909 1.7786

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization –
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
33 123 1588 747 1079 97 1214 180 1112 220 2802 927

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1354.09 2333.90 2229.75 2215.34 1838.27 2120.12 2448.45 2356.11 1823.90 2239.64 2324.50 2242.67
Standard Deviation 623.90 558.97 528.92 604.15 461.29 443.88 554.97 673.87 474.11 522.33 551.12 620.81
Intensity Change Log 2 — 0.785419 0.719558 0.710205 — 0.205797 0.413520 0.358058 — 0.480485 0.551330 0.518028

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000940 0.000640 0.000599 0.003866 0.000634 0.001554 0.000743 0.007169 0.000643 0.001043 0.000661 0.004508
Bud Neck 0.004746 0.004810 0.017594 0.030999 0.004064 0.014190 0.018679 0.027072 0.004084 0.008946 0.018064 0.030237
Bud Site 0.002487 0.000549 0.001441 0.007926 0.000237 0.002565 0.002030 0.014555 0.000303 0.001438 0.001697 0.009213
Cell Periphery 0.000690 0.000043 0.000116 0.001201 0.000090 0.000213 0.000184 0.001940 0.000108 0.000118 0.000145 0.001344
Cytoplasm 0.171273* 0.000441 0.001029 0.004959 0.000349 0.000480 0.002540 0.004388 0.005422 0.000458 0.001684 0.004848
Cytoplasmic Foci 0.015733 0.003253 0.001058 0.003875 0.000089 0.000254 0.002054 0.006443 0.000553 0.001931 0.001489 0.004374
Eisosomes 0.000059 0.000043 0.000046 0.000039 0.000053 0.000051 0.000046 0.000334 0.000053 0.000047 0.000046 0.000096
Endoplasmic Reticulum 0.026166 0.000498 0.000762 0.001354 0.000762 0.002599 0.000668 0.002664 0.001516 0.001424 0.000721 0.001609
Endosome 0.006345 0.000565 0.001494 0.008434 0.000018 0.000627 0.001222 0.005833 0.000206 0.000592 0.001377 0.007929
Golgi 0.001759 0.001935 0.000543 0.009095 0.000032 0.000302 0.001449 0.006565 0.000083 0.001215 0.000935 0.008604
Lipid Particles 0.008036 0.000118 0.000105 0.000394 0.000008 0.000426 0.000187 0.004587 0.000247 0.000254 0.000141 0.001208
Mitochondria 0.005332 0.001395 0.002353 0.008313 0.001424 0.002248 0.003821 0.004829 0.001540 0.001771 0.002989 0.007636
Mitotic Spindle 0.000591 0.000998 0.002818 0.031248 0.000094 0.052299 0.002284 0.014539 0.000109 0.023617 0.002587 0.028004
None 0.005747 0.000616 0.001426 0.006054 0.002416 0.001093 0.002131 0.003376 0.002515 0.000827 0.001732 0.005534
Nuclear Periphery 0.008251 0.000534 0.000319 0.004709 0.000144 0.001249 0.000734 0.002129 0.000384 0.000849 0.000499 0.004208
Nuclear Periphery Foci 0.028881 0.000317 0.000184 0.002389 0.000029 0.002832 0.000439 0.003489 0.000885 0.001426 0.000294 0.002603
Nucleolus 0.037718 0.015264 0.008879 0.028820 0.023434 0.022397 0.013185 0.020085 0.023858 0.018409 0.010745 0.027124
Nucleus 0.537921* 0.965742* 0.957740* 0.831099* 0.964611* 0.889771* 0.945290* 0.859469* 0.951949* 0.932246* 0.952346* 0.836608*
Peroxisomes 0.003382 0.001134 0.000418 0.001276 0.000896 0.001355 0.000569 0.001273 0.000970 0.001232 0.000484 0.001276
Vacuole 0.121153 0.000867 0.000660 0.008740 0.000402 0.002931 0.001106 0.008462 0.003985 0.001777 0.000853 0.008686
Vacuole Periphery 0.012791 0.000236 0.000416 0.005207 0.000214 0.000564 0.000639 0.000797 0.000587 0.000381 0.000513 0.004351

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.54 1.45 -1.48 -1.72 -1.68 -2.21 -4.35 -1.43 -0.87 -1.36 -2.14 -3.33 -2.18 -1.58 -2.09
Bud Neck -0.10 -8.58 -11.61 -12.99 -6.65 -3.34 -16.51 -8.13 -3.71 -3.29 -3.46 -21.33 -15.62 -10.47 -7.36
Bud Site 2.80 1.05 -3.55 -5.14 -4.31 -2.16 -5.97 -2.99 -1.82 -2.40 -2.25 -8.30 -6.18 -4.27 -4.84
Cell Periphery 1.72 1.51 -0.33 -1.92 -1.74 -1.39 -2.17 -1.32 -1.14 -1.19 -0.36 -1.31 -2.14 -2.08 -2.03
Cytoplasm 3.43 3.41 3.33 -6.03 -4.78 -1.11 -3.08 -4.73 -4.34 -1.46 2.86 2.00 0.15 -6.95 -4.25
Cytoplasmic Foci 1.81 2.54 2.14 0.09 -2.41 -2.01 -3.85 -1.86 -1.72 -0.95 -0.81 -2.40 -3.37 -0.58 -2.29
Eisosomes 1.04 0.69 1.28 0.56 2.19 -0.21 0.61 -0.97 -0.96 -0.99 0.82 0.75 -0.57 -0.75 -0.71
Endoplasmic Reticulum 3.78 3.74 3.67 -2.94 -1.99 -1.61 0.83 -1.55 0.80 -1.65 -0.40 3.11 0.28 0.52 -2.70
Endosome 3.03 2.75 0.62 -2.96 -2.61 -2.02 -3.05 -1.82 -1.33 -1.27 -1.35 -2.55 -3.86 -3.26 -2.98
Golgi -0.15 1.63 -1.83 -1.23 -2.64 -2.45 -1.95 -1.20 -1.05 -0.81 -1.12 -2.42 -3.08 -1.89 -2.63
Lipid Particles 2.01 2.02 1.96 -1.37 -2.30 -1.85 -2.40 -1.38 -1.07 -1.29 -0.46 0.88 -1.35 -1.09 -1.69
Mitochondria 1.26 0.86 -0.99 -6.56 -5.36 -2.43 -3.76 -7.43 -4.73 -2.44 -1.52 -4.86 -7.51 -6.89 -5.26
Mitotic Spindle -0.77 -2.28 -6.58 -6.22 -6.10 -2.95 -2.58 -3.08 1.49 -2.80 -2.96 -3.85 -7.35 -0.40 -6.73
None 2.25 1.77 -0.03 -3.96 -3.12 1.29 0.40 -1.61 -4.55 -3.32 1.73 0.73 -1.98 -4.41 -3.44
Nuclear Periphery 2.15 2.39 0.69 -4.30 -7.03 -2.05 -3.38 -3.47 -1.84 -2.68 -1.44 -1.66 -7.61 -4.94 -7.25
Nuclear Periphery Foci 2.39 2.42 2.25 -2.93 -4.53 -1.31 -2.50 -1.62 0.11 -1.41 -0.81 1.52 -2.30 -0.27 -3.83
Nucleolus 1.16 1.40 0.05 -3.68 -6.93 -0.27 2.63 -0.85 -0.32 -2.04 0.85 4.33 -2.91 -2.82 -6.83
Nucleus -6.68 -6.54 -4.53 9.95 12.22 3.41 6.14 6.36 0.65 4.85 2.11 1.64 12.92 5.52 12.81
Peroxisomes 0.84 1.10 0.78 -0.34 -3.67 -1.55 1.72 -1.47 0.81 -2.17 -1.23 3.20 -1.61 0.34 -3.88
Vacuole 4.05 4.05 3.87 -3.87 -4.35 -1.83 -2.96 -2.10 -0.69 -1.69 1.49 2.78 -1.23 -2.87 -4.48
Vacuole Periphery 2.76 2.74 1.98 -2.82 -2.72 -1.84 -1.50 -3.25 0.05 -0.43 0.74 0.74 -2.53 -2.69 -2.69
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Rpt4

Rpt4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpt4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available