Standard name | |
---|---|
Human Ortholog | |
Description | Gamma subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 5 | 13 | 7 | 11 | 25 | 20 | 47 | 77 | 71 | 80 | 96 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 4 | 4 | 1 | 0 | 1 |
Bud | 0 | 6 | 2 | 0 | 0 | 0 | 0 | 2 | 7 | 3 | 18 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 4 | 1 | 3 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 24 | 40 | 46 | 50 | 64 | 65 | 108 | 160 | 123 | 134 | 155 | 2 | 3 | 13 | 6 | 4 | 7 | 0 | 0 | 11 | 0 | 0 | 0 |
Cytoplasm | 164 | 942 | 1098 | 786 | 773 | 789 | 783 | 718 | 814 | 685 | 751 | 626 | 168 | 305 | 274 | 101 | 70 | 70 | 163 | 956 | 925 | 204 | 369 | 303 |
Endoplasmic Reticulum | 4 | 59 | 157 | 67 | 70 | 214 | 191 | 95 | 161 | 160 | 156 | 120 | 41 | 43 | 33 | 9 | 2 | 5 | 1 | 27 | 156 | 52 | 78 | 57 |
Endosome | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 3 | 2 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 1 | 0 |
Golgi | 0 | 2 | 48 | 9 | 33 | 4 | 23 | 52 | 34 | 30 | 12 | 32 | 5 | 7 | 5 | 1 | 1 | 1 | 1 | 2 | 22 | 4 | 7 | 5 |
Mitochondria | 1 | 2 | 8 | 9 | 77 | 289 | 51 | 26 | 242 | 169 | 402 | 247 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 12 | 7 | 4 | 5 |
Nucleus | 1 | 0 | 1 | 0 | 3 | 3 | 7 | 6 | 1 | 2 | 3 | 2 | 0 | 1 | 2 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 2 | 16 | 1 | 1 | 1 | 12 | 3 | 14 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 0 | 2 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | 0 | 4 | 30 | 24 | 114 | 38 | 59 | 111 | 117 | 78 | 56 | 116 | 13 | 26 | 13 | 4 | 2 | 2 | 3 | 16 | 13 | 18 | 24 | 14 |
Unique Cell Count | 169 | 1003 | 1304 | 887 | 1043 | 1258 | 1068 | 1028 | 1317 | 1119 | 1303 | 1107 | 218 | 370 | 324 | 109 | 76 | 76 | 173 | 1028 | 1172 | 297 | 500 | 396 |
Labelled Cell Count | 173 | 1044 | 1398 | 949 | 1137 | 1443 | 1201 | 1166 | 1618 | 1336 | 1619 | 1411 | 232 | 386 | 342 | 122 | 81 | 86 | 173 | 1028 | 1172 | 297 | 500 | 396 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 20.5 | 20.8 | 18.7 | 16.3 | 13.8 | 11.4 | 12.9 | 15.5 | 12.0 | 12.3 | 11.0 | 11.5 | 19.0 | 19.8 | 19.5 | 20.8 | 19.9 | 20.2 | 17.4 | 17.9 | 19.1 |
Std Deviation (1e-4) | 3.0 | 3.4 | 3.3 | 2.6 | 2.6 | 2.6 | 2.9 | 2.5 | 2.6 | 2.4 | 2.3 | 2.3 | 3.6 | 3.2 | 3.7 | 6.3 | 6.2 | 6.3 | 3.3 | 3.5 | 3.8 |
Intensity Change (Log2) | – | – | – | -0.2 | -0.45 | -0.72 | -0.54 | -0.28 | -0.64 | -0.61 | -0.76 | -0.7 | 0.02 | 0.08 | 0.06 | 0.15 | 0.09 | 0.11 | -0.11 | -0.06 | 0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.5 | 0.1 | 2.1 | 1.8 | 5.4 | 6.8 | 7.2 | 7.1 | 9.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.5 | 2.2 | 2.6 | 3.6 | 7.3 | 8.8 | 7.8 | 7.4 | 9.8 | -1.8 | -2.4 | 0.9 | 0 | 0 | 0 |
Cytoplasm | 2.9 | -6.0 | -12.3 | -6.5 | -8.3 | -12.9 | -12.8 | -14.9 | -15.0 | -2.6 | -0.8 | 0.2 | 2.4 | 1.9 | 1.9 |
Endoplasmic Reticulum | -3.4 | -4.3 | 3.6 | 4.0 | -2.2 | 0.1 | 1.6 | -0.1 | -0.9 | 2.7 | -0.2 | -0.9 | -1.2 | -2.5 | -1.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -3.8 | -0.7 | -6.0 | -2.2 | 1.6 | -1.6 | -1.4 | -4.7 | -1.1 | -1.0 | -1.7 | -1.9 | 0 | 0 | 0 |
Mitochondria | 1.1 | 8.7 | 17.7 | 6.5 | 3.8 | 15.5 | 13.7 | 21.2 | 17.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 4.1 | 0 | 0 | 0 | 3.7 | 0 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.6 | 8.7 | 1.1 | 4.1 | 8.5 | 7.3 | 5.6 | 2.9 | 8.4 | 3.0 | 4.5 | 1.7 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.3575 | 17.1217 | 14.5364 | 13.5065 | 14.6242 | 14.6142 | 14.9668 | 19.5213 | 17.9246 | 16.1415 | 16.5365 | 17.6181 | 10.9471 | 17.2819 | 15.7923 | 13.1162 | 14.031 | 13.9975 |
Actin | 0.0042 | 0.0023 | 0.0023 | 0.0019 | 0.0045 | 0.0027 | 0.0048 | 0.0014 | 0.0022 | 0.0031 | 0.0021 | 0.0014 | 0.0201 | 0.0018 | 0.0139 | 0.0962 | 0.0183 | 0.0013 |
Bud | 0.0013 | 0.001 | 0.0019 | 0.0017 | 0.001 | 0.0022 | 0.0014 | 0.0011 | 0.0015 | 0.0022 | 0.0007 | 0.0009 | 0.0019 | 0.0008 | 0.0013 | 0.0026 | 0.0012 | 0.0004 |
Bud Neck | 0.0006 | 0.0005 | 0.0006 | 0.0006 | 0.0006 | 0.0018 | 0.0007 | 0.0006 | 0.0008 | 0.001 | 0.0009 | 0.0013 | 0.0009 | 0.0003 | 0.0006 | 0.0009 | 0.0003 | 0.0008 |
Bud Periphery | 0.0011 | 0.0004 | 0.0008 | 0.0005 | 0.0003 | 0.0007 | 0.001 | 0.0007 | 0.0008 | 0.0012 | 0.0002 | 0.0005 | 0.0016 | 0.0001 | 0.0007 | 0.0008 | 0.0014 | 0.0001 |
Bud Site | 0.0011 | 0.0015 | 0.0013 | 0.0007 | 0.0003 | 0.0003 | 0.0013 | 0.001 | 0.001 | 0.0013 | 0.0003 | 0.0002 | 0.0056 | 0.0018 | 0.001 | 0.002 | 0.0004 | 0.0001 |
Cell Periphery | 0.0012 | 0.0005 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0005 | 0.0004 | 0.0003 | 0.0002 | 0.0004 | 0.0005 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0 |
Cytoplasm | 0.6268 | 0.8193 | 0.7409 | 0.6658 | 0.5703 | 0.6443 | 0.6597 | 0.7984 | 0.6683 | 0.5814 | 0.5527 | 0.6616 | 0.5813 | 0.8573 | 0.7143 | 0.4627 | 0.5716 | 0.6418 |
Cytoplasmic Foci | 0.0293 | 0.0254 | 0.0317 | 0.0361 | 0.0467 | 0.0379 | 0.0375 | 0.0297 | 0.0396 | 0.0491 | 0.0537 | 0.0476 | 0.0407 | 0.0235 | 0.0346 | 0.0452 | 0.0266 | 0.0298 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0623 | 0.0161 | 0.0154 | 0.0197 | 0.0056 | 0.0348 | 0.0264 | 0.0231 | 0.0151 | 0.0129 | 0.0198 | 0.0338 | 0.0644 | 0.0103 | 0.0163 | 0.0036 | 0.0047 | 0.0509 |
Endosome | 0.1557 | 0.0914 | 0.142 | 0.1883 | 0.2791 | 0.1974 | 0.1646 | 0.092 | 0.1684 | 0.2492 | 0.2623 | 0.1667 | 0.154 | 0.0635 | 0.1363 | 0.2764 | 0.2094 | 0.1867 |
Golgi | 0.0321 | 0.0176 | 0.0171 | 0.0138 | 0.065 | 0.0419 | 0.0549 | 0.0263 | 0.056 | 0.0388 | 0.078 | 0.0555 | 0.0428 | 0.0195 | 0.0269 | 0.0359 | 0.0262 | 0.0572 |
Lipid Particles | 0.0013 | 0.0006 | 0.0002 | 0.0178 | 0.0001 | 0.0001 | 0.0006 | 0.0004 | 0.0004 | 0.0003 | 0.0005 | 0.0002 | 0.0021 | 0.0001 | 0.002 | 0.0005 | 0.0005 | 0.0003 |
Mitochondria | 0.016 | 0.0041 | 0.0059 | 0.0022 | 0.0077 | 0.011 | 0.0101 | 0.0071 | 0.0063 | 0.0074 | 0.0057 | 0.0128 | 0.0276 | 0.0017 | 0.0116 | 0.0038 | 0.0951 | 0.0043 |
None | 0.004 | 0.0004 | 0.0006 | 0.0005 | 0.0003 | 0.0005 | 0.0008 | 0.0004 | 0.0005 | 0.0005 | 0.0004 | 0.0005 | 0.0239 | 0.0004 | 0.0008 | 0.0004 | 0.0003 | 0.0005 |
Nuclear Periphery | 0.0095 | 0.0015 | 0.0023 | 0.0044 | 0.0009 | 0.0024 | 0.0022 | 0.0009 | 0.0014 | 0.0017 | 0.0013 | 0.001 | 0.007 | 0.0033 | 0.0035 | 0.0043 | 0.0025 | 0.0027 |
Nucleolus | 0.0004 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 |
Nucleus | 0.0027 | 0.001 | 0.0013 | 0.0019 | 0.0005 | 0.0013 | 0.0011 | 0.0007 | 0.0009 | 0.0011 | 0.0008 | 0.0009 | 0.0019 | 0.0009 | 0.0013 | 0.002 | 0.0007 | 0.001 |
Peroxisomes | 0.0003 | 0.0002 | 0.0002 | 0.0045 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0014 | 0.0001 | 0.0009 | 0.0007 | 0.0002 | 0.0002 |
Punctate Nuclear | 0.0025 | 0.0001 | 0.0002 | 0.0022 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Vacuole | 0.038 | 0.0124 | 0.0262 | 0.0245 | 0.0097 | 0.0126 | 0.0242 | 0.0122 | 0.0273 | 0.0356 | 0.0124 | 0.0094 | 0.0145 | 0.01 | 0.0234 | 0.0434 | 0.0242 | 0.0127 |
Vacuole Periphery | 0.0097 | 0.0038 | 0.0089 | 0.0126 | 0.0071 | 0.0075 | 0.0072 | 0.003 | 0.0089 | 0.0126 | 0.0076 | 0.0048 | 0.0064 | 0.0043 | 0.0098 | 0.0179 | 0.0157 | 0.0092 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 84.4864 | 60.7612 | 80.0618 | 84.407 | 84.4201 | 62.8535 | 68.0385 | 80.9922 | 71.5072 | 81.9654 |
Translational Efficiency | 0.8312 | 0.9127 | 0.7785 | 0.676 | 0.6174 | 0.8924 | 0.7621 | 0.5615 | 0.6704 | 0.6768 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1649 | 1875 | 3150 | 1329 | 155 | 68 | 2209 | 2200 | 1804 | 1943 | 5359 | 3529 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1367.72 | 1079.85 | 1607.35 | 1626.01 | 1467.73 | 1536.82 | 1577.52 | 1397.09 | 1376.31 | 1095.84 | 1595.05 | 1483.30 |
Standard Deviation | 269.99 | 213.84 | 225.99 | 298.73 | 269.44 | 294.00 | 227.90 | 224.58 | 271.40 | 232.82 | 227.26 | 278.12 |
Intensity Change Log 2 | — | -0.340942 | 0.232911 | 0.249563 | — | 0.066362 | 0.104072 | -0.071162 | — | -0.115846 | 0.167657 | 0.092451 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000418 | 0.000381 | 0.000220 | 0.000927 | 0.001255 | 0.001557 | 0.000238 | 0.000262 | 0.000490 | 0.000422 | 0.000228 | 0.000513 |
Bud Neck | 0.000410 | 0.000488 | 0.000481 | 0.001779 | 0.000925 | 0.000411 | 0.000415 | 0.000515 | 0.000454 | 0.000486 | 0.000454 | 0.000991 |
Bud Site | 0.035818 | 0.041369 | 0.030002 | 0.071018 | 0.075328 | 0.078190 | 0.025470 | 0.034932 | 0.039212 | 0.042658 | 0.028134 | 0.048521 |
Cell Periphery | 0.001019 | 0.001121 | 0.001125 | 0.006754 | 0.004218 | 0.003836 | 0.001326 | 0.002905 | 0.001294 | 0.001216 | 0.001208 | 0.004354 |
Cytoplasm | 0.526522* | 0.427784* | 0.677499* | 0.646783* | 0.387708* | 0.232177* | 0.688521* | 0.604127* | 0.514595* | 0.420938* | 0.682042* | 0.620191* |
Cytoplasmic Foci | 0.206348* | 0.224326* | 0.103219 | 0.061254 | 0.231462* | 0.216171* | 0.085298 | 0.118422 | 0.208506* | 0.224040* | 0.095832 | 0.096893 |
Eisosomes | 0.000012 | 0.000014 | 0.000010 | 0.000010 | 0.000016 | 0.000026 | 0.000005 | 0.000007 | 0.000012 | 0.000015 | 0.000008 | 0.000008 |
Endoplasmic Reticulum | 0.005509 | 0.007625 | 0.001127 | 0.002061 | 0.008599 | 0.004779 | 0.000910 | 0.006748 | 0.005775 | 0.007526 | 0.001037 | 0.004983 |
Endosome | 0.016966 | 0.029031 | 0.007586 | 0.011166 | 0.028865 | 0.038991 | 0.007907 | 0.014240 | 0.017988 | 0.029380 | 0.007718 | 0.013082 |
Golgi | 0.108452 | 0.097443 | 0.082124 | 0.072526 | 0.160259* | 0.293900* | 0.079820 | 0.065136 | 0.112903* | 0.104318 | 0.081174 | 0.067919 |
Lipid Particles | 0.002204 | 0.001969 | 0.002488 | 0.011896 | 0.005924 | 0.003058 | 0.003649 | 0.004192 | 0.002524 | 0.002007 | 0.002967 | 0.007093 |
Mitochondria | 0.003730 | 0.004308 | 0.004882 | 0.000817 | 0.001178 | 0.014636 | 0.001821 | 0.000905 | 0.003511 | 0.004670 | 0.003620 | 0.000872 |
Mitotic Spindle | 0.001861 | 0.001503 | 0.001532 | 0.002629 | 0.011139 | 0.014704 | 0.002613 | 0.001332 | 0.002658 | 0.001965 | 0.001978 | 0.001821 |
None | 0.000842 | 0.000590 | 0.000209 | 0.000387 | 0.000197 | 0.000283 | 0.000167 | 0.000163 | 0.000786 | 0.000580 | 0.000191 | 0.000248 |
Nuclear Periphery | 0.000035 | 0.000098 | 0.000017 | 0.000044 | 0.000152 | 0.000169 | 0.000028 | 0.000032 | 0.000045 | 0.000101 | 0.000022 | 0.000036 |
Nuclear Periphery Foci | 0.001039 | 0.001678 | 0.000742 | 0.001595 | 0.002209 | 0.001419 | 0.001051 | 0.001067 | 0.001140 | 0.001669 | 0.000869 | 0.001266 |
Nucleolus | 0.000048 | 0.000086 | 0.000049 | 0.000103 | 0.000180 | 0.000177 | 0.000050 | 0.000053 | 0.000060 | 0.000089 | 0.000049 | 0.000072 |
Nucleus | 0.000723 | 0.000988 | 0.000768 | 0.004101 | 0.006596 | 0.001408 | 0.000951 | 0.000754 | 0.001228 | 0.001003 | 0.000843 | 0.002015 |
Peroxisomes | 0.000415 | 0.000488 | 0.000550 | 0.001168 | 0.000817 | 0.000781 | 0.000369 | 0.000474 | 0.000450 | 0.000498 | 0.000476 | 0.000735 |
Vacuole | 0.030249 | 0.056628 | 0.034038 | 0.064728 | 0.030638 | 0.031886 | 0.049859 | 0.068389 | 0.030283 | 0.055762 | 0.040560 | 0.067010 |
Vacuole Periphery | 0.057380 | 0.102081* | 0.051331 | 0.038253 | 0.042336 | 0.061440 | 0.049532 | 0.075346 | 0.056087 | 0.100658* | 0.050589 | 0.061377 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.36 | 2.35 | -4.16 | -4.77 | -6.92 | -0.46 | 4.20 | 4.14 | 2.16 | -0.48 | 0.67 | 3.28 | -0.47 | -1.39 | -6.38 |
Bud Neck | -1.83 | -1.33 | -10.81 | -10.20 | -9.94 | 3.88 | 4.04 | 3.21 | -1.55 | -1.82 | -0.76 | -0.15 | -8.99 | -8.45 | -8.86 |
Bud Site | -2.47 | 3.36 | -10.69 | -8.71 | -13.20 | -0.17 | 6.57 | 5.30 | 3.37 | -5.38 | -1.51 | 6.64 | -4.58 | -2.72 | -12.80 |
Cell Periphery | -0.46 | -0.85 | -7.13 | -6.81 | -6.98 | 0.23 | 2.47 | 1.10 | 0.70 | -4.86 | 0.34 | 0.58 | -8.23 | -7.75 | -8.72 |
Cytoplasm | 8.46 | -14.94 | -9.70 | -18.34 | 2.96 | 3.39 | -10.77 | -7.74 | -10.19 | 8.77 | 8.26 | -18.13 | -10.66 | -20.90 | 8.98 |
Cytoplasmic Foci | -2.58 | 18.79 | 25.64 | 29.73 | 12.18 | 0.64 | 9.19 | 7.06 | 4.54 | -8.37 | -2.31 | 22.28 | 21.13 | 24.36 | -0.44 |
Eisosomes | -1.72 | 1.10 | 1.19 | 2.65 | -0.15 | -1.53 | 3.28 | 2.74 | 3.42 | -3.06 | -1.79 | 4.06 | 4.75 | 5.35 | -0.07 |
Endoplasmic Reticulum | -2.14 | 6.15 | 4.47 | 7.34 | -2.71 | 1.07 | 2.24 | 0.53 | -1.61 | -9.02 | -1.79 | 6.63 | 0.98 | 3.24 | -9.28 |
Endosome | -9.78 | 11.96 | 6.02 | 15.18 | -4.84 | -1.74 | 6.25 | 4.33 | 5.25 | -9.60 | -9.36 | 13.38 | 5.81 | 15.43 | -11.10 |
Golgi | 2.29 | 5.98 | 7.06 | 5.38 | 2.47 | -4.07 | 5.73 | 6.82 | 7.71 | 3.92 | 1.78 | 7.88 | 11.06 | 9.62 | 5.03 |
Lipid Particles | 0.86 | -1.12 | -8.18 | -8.34 | -7.86 | 2.07 | 1.86 | 1.44 | -1.29 | -0.93 | 1.87 | -1.74 | -8.45 | -9.33 | -7.50 |
Mitochondria | -1.03 | -2.42 | 7.29 | 7.67 | 12.34 | -1.02 | -1.20 | 0.57 | 1.04 | 4.14 | -1.64 | -0.25 | 7.58 | 6.12 | 13.34 |
Mitotic Spindle | 0.70 | 0.92 | -1.38 | -1.85 | -2.07 | -0.25 | 1.73 | 2.01 | 1.04 | 3.76 | 0.88 | 1.21 | 1.43 | 0.23 | 0.55 |
None | 0.60 | 1.64 | 1.09 | 1.08 | -3.09 | -0.67 | 0.82 | 0.91 | 0.97 | 0.26 | 0.54 | 1.69 | 1.50 | 2.21 | -2.47 |
Nuclear Periphery | -3.10 | 1.82 | -1.25 | 2.66 | -6.18 | -0.11 | 1.33 | 1.30 | 1.12 | -0.65 | -2.60 | 1.91 | 0.57 | 3.48 | -5.77 |
Nuclear Periphery Foci | -2.22 | 1.33 | -2.53 | 0.27 | -5.31 | 0.98 | 2.69 | 2.72 | 0.49 | 0.10 | -1.93 | 1.31 | -0.70 | 1.75 | -3.64 |
Nucleolus | -2.35 | -0.20 | -7.39 | -1.21 | -10.32 | 0.02 | 0.84 | 0.82 | 1.10 | -0.99 | -1.44 | 0.70 | -0.93 | 1.07 | -7.93 |
Nucleus | -1.88 | -0.73 | -5.34 | -4.94 | -5.29 | 0.84 | 0.92 | 0.95 | 0.76 | 1.36 | 0.40 | 0.71 | -1.84 | -4.06 | -4.85 |
Peroxisomes | -1.29 | -2.48 | -6.63 | -5.62 | -5.14 | 0.20 | 2.66 | 1.99 | 1.84 | -1.77 | -0.85 | -0.59 | -4.66 | -3.27 | -4.24 |
Vacuole | -9.62 | -1.86 | -10.26 | -2.41 | -9.65 | -0.10 | -3.96 | -7.26 | -3.56 | -5.32 | -9.63 | -5.35 | -14.71 | -4.13 | -11.62 |
Vacuole Periphery | -10.97 | 2.11 | 6.18 | 16.71 | 5.33 | -1.43 | -1.13 | -5.15 | -1.19 | -8.06 | -11.38 | 2.16 | -1.69 | 10.88 | -4.86 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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