Standard name
Human Ortholog
Description Gamma subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.05 0.06 0.06 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0.05 0.05 0.06 0.11 0.12 0.11 0.1 0.14 0 0 0 0.06 0.05 0.09 0 0 0 0 0 0
Cytoplasm 0.97 0.94 0.84 0.89 0.74 0.63 0.73 0.7 0.62 0.61 0.58 0.57 0.77 0.82 0.85 0.93 0.92 0.92 0.94 0.93 0.79 0.69 0.74 0.77
Endoplasmic Reticulum 0 0.06 0.12 0.08 0.07 0.17 0.18 0.09 0.12 0.14 0.12 0.11 0.19 0.12 0.1 0.08 0 0.07 0 0 0.13 0.18 0.16 0.15
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.07 0.23 0.05 0 0.18 0.15 0.31 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.11 0 0.06 0.11 0.09 0.07 0 0.1 0.06 0.07 0 0 0 0 0 0 0 0.06 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 5 13 7 11 25 20 47 77 71 80 96 2 0 1 0 1 0 0 4 4 1 0 1
Bud 0 6 2 0 0 0 0 2 7 3 18 3 0 0 0 0 0 0 1 3 4 1 3 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 24 40 46 50 64 65 108 160 123 134 155 2 3 13 6 4 7 0 0 11 0 0 0
Cytoplasm 164 942 1098 786 773 789 783 718 814 685 751 626 168 305 274 101 70 70 163 956 925 204 369 303
Endoplasmic Reticulum 4 59 157 67 70 214 191 95 161 160 156 120 41 43 33 9 2 5 1 27 156 52 78 57
Endosome 0 0 1 0 3 0 0 0 3 2 3 0 1 1 0 0 0 0 0 1 4 1 1 0
Golgi 0 2 48 9 33 4 23 52 34 30 12 32 5 7 5 1 1 1 1 2 22 4 7 5
Mitochondria 1 2 8 9 77 289 51 26 242 169 402 247 0 0 1 1 0 1 0 2 12 7 4 5
Nucleus 1 0 1 0 3 3 7 6 1 2 3 2 0 1 2 0 1 0 1 2 0 0 0 0
Nuclear Periphery 0 0 0 1 2 16 1 1 1 12 3 14 0 0 0 0 0 0 0 1 4 0 2 0
Nucleolus 0 0 0 0 0 1 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0
Vac/Vac Membrane 0 4 30 24 114 38 59 111 117 78 56 116 13 26 13 4 2 2 3 16 13 18 24 14
Unique Cell Count 169 1003 1304 887 1043 1258 1068 1028 1317 1119 1303 1107 218 370 324 109 76 76 173 1028 1172 297 500 396
Labelled Cell Count 173 1044 1398 949 1137 1443 1201 1166 1618 1336 1619 1411 232 386 342 122 81 86 173 1028 1172 297 500 396


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.5 20.8 18.7 16.3 13.8 11.4 12.9 15.5 12.0 12.3 11.0 11.5 19.0 19.8 19.5 20.8 19.9 20.2 17.4 17.9 19.1
Std Deviation (1e-4) 3.0 3.4 3.3 2.6 2.6 2.6 2.9 2.5 2.6 2.4 2.3 2.3 3.6 3.2 3.7 6.3 6.2 6.3 3.3 3.5 3.8
Intensity Change (Log2) – – – -0.2 -0.45 -0.72 -0.54 -0.28 -0.64 -0.61 -0.76 -0.7 0.02 0.08 0.06 0.15 0.09 0.11 -0.11 -0.06 0.03

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70001020WT3HU80HU120HU16001020WT3rpd3Δ_1rpd3Δ_2rpd3Δ_301020WT1AF100AF140AF18001020
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.5 0.1 2.1 1.8 5.4 6.8 7.2 7.1 9.0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 3.6 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.5 2.2 2.6 3.6 7.3 8.8 7.8 7.4 9.8 -1.8 -2.4 0.9 0 0 0
Cytoplasm 2.9 -6.0 -12.3 -6.5 -8.3 -12.9 -12.8 -14.9 -15.0 -2.6 -0.8 0.2 2.4 1.9 1.9
Endoplasmic Reticulum -3.4 -4.3 3.6 4.0 -2.2 0.1 1.6 -0.1 -0.9 2.7 -0.2 -0.9 -1.2 -2.5 -1.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -3.8 -0.7 -6.0 -2.2 1.6 -1.6 -1.4 -4.7 -1.1 -1.0 -1.7 -1.9 0 0 0
Mitochondria 1.1 8.7 17.7 6.5 3.8 15.5 13.7 21.2 17.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 4.1 0 0 0 3.7 0 4.1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.6 8.7 1.1 4.1 8.5 7.3 5.6 2.9 8.4 3.0 4.5 1.7 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.3575 17.1217 14.5364 13.5065 14.6242 14.6142 14.9668 19.5213 17.9246 16.1415 16.5365 17.6181 10.9471 17.2819 15.7923 13.1162 14.031 13.9975
Actin 0.0042 0.0023 0.0023 0.0019 0.0045 0.0027 0.0048 0.0014 0.0022 0.0031 0.0021 0.0014 0.0201 0.0018 0.0139 0.0962 0.0183 0.0013
Bud 0.0013 0.001 0.0019 0.0017 0.001 0.0022 0.0014 0.0011 0.0015 0.0022 0.0007 0.0009 0.0019 0.0008 0.0013 0.0026 0.0012 0.0004
Bud Neck 0.0006 0.0005 0.0006 0.0006 0.0006 0.0018 0.0007 0.0006 0.0008 0.001 0.0009 0.0013 0.0009 0.0003 0.0006 0.0009 0.0003 0.0008
Bud Periphery 0.0011 0.0004 0.0008 0.0005 0.0003 0.0007 0.001 0.0007 0.0008 0.0012 0.0002 0.0005 0.0016 0.0001 0.0007 0.0008 0.0014 0.0001
Bud Site 0.0011 0.0015 0.0013 0.0007 0.0003 0.0003 0.0013 0.001 0.001 0.0013 0.0003 0.0002 0.0056 0.0018 0.001 0.002 0.0004 0.0001
Cell Periphery 0.0012 0.0005 0.0004 0.0001 0.0001 0.0001 0.0009 0.0005 0.0004 0.0003 0.0002 0.0004 0.0005 0.0002 0.0004 0.0002 0.0001 0
Cytoplasm 0.6268 0.8193 0.7409 0.6658 0.5703 0.6443 0.6597 0.7984 0.6683 0.5814 0.5527 0.6616 0.5813 0.8573 0.7143 0.4627 0.5716 0.6418
Cytoplasmic Foci 0.0293 0.0254 0.0317 0.0361 0.0467 0.0379 0.0375 0.0297 0.0396 0.0491 0.0537 0.0476 0.0407 0.0235 0.0346 0.0452 0.0266 0.0298
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0 0.0003 0 0.0001 0.0003 0.0003 0
Endoplasmic Reticulum 0.0623 0.0161 0.0154 0.0197 0.0056 0.0348 0.0264 0.0231 0.0151 0.0129 0.0198 0.0338 0.0644 0.0103 0.0163 0.0036 0.0047 0.0509
Endosome 0.1557 0.0914 0.142 0.1883 0.2791 0.1974 0.1646 0.092 0.1684 0.2492 0.2623 0.1667 0.154 0.0635 0.1363 0.2764 0.2094 0.1867
Golgi 0.0321 0.0176 0.0171 0.0138 0.065 0.0419 0.0549 0.0263 0.056 0.0388 0.078 0.0555 0.0428 0.0195 0.0269 0.0359 0.0262 0.0572
Lipid Particles 0.0013 0.0006 0.0002 0.0178 0.0001 0.0001 0.0006 0.0004 0.0004 0.0003 0.0005 0.0002 0.0021 0.0001 0.002 0.0005 0.0005 0.0003
Mitochondria 0.016 0.0041 0.0059 0.0022 0.0077 0.011 0.0101 0.0071 0.0063 0.0074 0.0057 0.0128 0.0276 0.0017 0.0116 0.0038 0.0951 0.0043
None 0.004 0.0004 0.0006 0.0005 0.0003 0.0005 0.0008 0.0004 0.0005 0.0005 0.0004 0.0005 0.0239 0.0004 0.0008 0.0004 0.0003 0.0005
Nuclear Periphery 0.0095 0.0015 0.0023 0.0044 0.0009 0.0024 0.0022 0.0009 0.0014 0.0017 0.0013 0.001 0.007 0.0033 0.0035 0.0043 0.0025 0.0027
Nucleolus 0.0004 0 0.0001 0.0001 0 0 0 0 0 0 0 0 0.0002 0 0 0.0001 0 0
Nucleus 0.0027 0.001 0.0013 0.0019 0.0005 0.0013 0.0011 0.0007 0.0009 0.0011 0.0008 0.0009 0.0019 0.0009 0.0013 0.002 0.0007 0.001
Peroxisomes 0.0003 0.0002 0.0002 0.0045 0.0003 0.0003 0.0002 0.0002 0.0002 0.0003 0.0004 0.0002 0.0014 0.0001 0.0009 0.0007 0.0002 0.0002
Punctate Nuclear 0.0025 0.0001 0.0002 0.0022 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0009 0.0001 0.0002 0.0002 0.0001 0.0001
Vacuole 0.038 0.0124 0.0262 0.0245 0.0097 0.0126 0.0242 0.0122 0.0273 0.0356 0.0124 0.0094 0.0145 0.01 0.0234 0.0434 0.0242 0.0127
Vacuole Periphery 0.0097 0.0038 0.0089 0.0126 0.0071 0.0075 0.0072 0.003 0.0089 0.0126 0.0076 0.0048 0.0064 0.0043 0.0098 0.0179 0.0157 0.0092

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 84.4864 60.7612 80.0618 84.407 84.4201 62.8535 68.0385 80.9922 71.5072 81.9654
Translational Efficiency 0.8312 0.9127 0.7785 0.676 0.6174 0.8924 0.7621 0.5615 0.6704 0.6768

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1649 1875 3150 1329 155 68 2209 2200 1804 1943 5359 3529

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1367.72 1079.85 1607.35 1626.01 1467.73 1536.82 1577.52 1397.09 1376.31 1095.84 1595.05 1483.30
Standard Deviation 269.99 213.84 225.99 298.73 269.44 294.00 227.90 224.58 271.40 232.82 227.26 278.12
Intensity Change Log 2 — -0.340942 0.232911 0.249563 — 0.066362 0.104072 -0.071162 — -0.115846 0.167657 0.092451

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000418 0.000381 0.000220 0.000927 0.001255 0.001557 0.000238 0.000262 0.000490 0.000422 0.000228 0.000513
Bud Neck 0.000410 0.000488 0.000481 0.001779 0.000925 0.000411 0.000415 0.000515 0.000454 0.000486 0.000454 0.000991
Bud Site 0.035818 0.041369 0.030002 0.071018 0.075328 0.078190 0.025470 0.034932 0.039212 0.042658 0.028134 0.048521
Cell Periphery 0.001019 0.001121 0.001125 0.006754 0.004218 0.003836 0.001326 0.002905 0.001294 0.001216 0.001208 0.004354
Cytoplasm 0.526522 0.427784 0.677499 0.646783 0.387708 0.232177 0.688521 0.604127 0.514595 0.420938 0.682042 0.620191
Cytoplasmic Foci 0.206348 0.224326 0.103219 0.061254 0.231462 0.216171 0.085298 0.118422 0.208506 0.224040 0.095832 0.096893
Eisosomes 0.000012 0.000014 0.000010 0.000010 0.000016 0.000026 0.000005 0.000007 0.000012 0.000015 0.000008 0.000008
Endoplasmic Reticulum 0.005509 0.007625 0.001127 0.002061 0.008599 0.004779 0.000910 0.006748 0.005775 0.007526 0.001037 0.004983
Endosome 0.016966 0.029031 0.007586 0.011166 0.028865 0.038991 0.007907 0.014240 0.017988 0.029380 0.007718 0.013082
Golgi 0.108452 0.097443 0.082124 0.072526 0.160259 0.293900 0.079820 0.065136 0.112903 0.104318 0.081174 0.067919
Lipid Particles 0.002204 0.001969 0.002488 0.011896 0.005924 0.003058 0.003649 0.004192 0.002524 0.002007 0.002967 0.007093
Mitochondria 0.003730 0.004308 0.004882 0.000817 0.001178 0.014636 0.001821 0.000905 0.003511 0.004670 0.003620 0.000872
Mitotic Spindle 0.001861 0.001503 0.001532 0.002629 0.011139 0.014704 0.002613 0.001332 0.002658 0.001965 0.001978 0.001821
None 0.000842 0.000590 0.000209 0.000387 0.000197 0.000283 0.000167 0.000163 0.000786 0.000580 0.000191 0.000248
Nuclear Periphery 0.000035 0.000098 0.000017 0.000044 0.000152 0.000169 0.000028 0.000032 0.000045 0.000101 0.000022 0.000036
Nuclear Periphery Foci 0.001039 0.001678 0.000742 0.001595 0.002209 0.001419 0.001051 0.001067 0.001140 0.001669 0.000869 0.001266
Nucleolus 0.000048 0.000086 0.000049 0.000103 0.000180 0.000177 0.000050 0.000053 0.000060 0.000089 0.000049 0.000072
Nucleus 0.000723 0.000988 0.000768 0.004101 0.006596 0.001408 0.000951 0.000754 0.001228 0.001003 0.000843 0.002015
Peroxisomes 0.000415 0.000488 0.000550 0.001168 0.000817 0.000781 0.000369 0.000474 0.000450 0.000498 0.000476 0.000735
Vacuole 0.030249 0.056628 0.034038 0.064728 0.030638 0.031886 0.049859 0.068389 0.030283 0.055762 0.040560 0.067010
Vacuole Periphery 0.057380 0.102081 0.051331 0.038253 0.042336 0.061440 0.049532 0.075346 0.056087 0.100658 0.050589 0.061377

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.36 2.35 -4.16 -4.77 -6.92 -0.46 4.20 4.14 2.16 -0.48 0.67 3.28 -0.47 -1.39 -6.38
Bud Neck -1.83 -1.33 -10.81 -10.20 -9.94 3.88 4.04 3.21 -1.55 -1.82 -0.76 -0.15 -8.99 -8.45 -8.86
Bud Site -2.47 3.36 -10.69 -8.71 -13.20 -0.17 6.57 5.30 3.37 -5.38 -1.51 6.64 -4.58 -2.72 -12.80
Cell Periphery -0.46 -0.85 -7.13 -6.81 -6.98 0.23 2.47 1.10 0.70 -4.86 0.34 0.58 -8.23 -7.75 -8.72
Cytoplasm 8.46 -14.94 -9.70 -18.34 2.96 3.39 -10.77 -7.74 -10.19 8.77 8.26 -18.13 -10.66 -20.90 8.98
Cytoplasmic Foci -2.58 18.79 25.64 29.73 12.18 0.64 9.19 7.06 4.54 -8.37 -2.31 22.28 21.13 24.36 -0.44
Eisosomes -1.72 1.10 1.19 2.65 -0.15 -1.53 3.28 2.74 3.42 -3.06 -1.79 4.06 4.75 5.35 -0.07
Endoplasmic Reticulum -2.14 6.15 4.47 7.34 -2.71 1.07 2.24 0.53 -1.61 -9.02 -1.79 6.63 0.98 3.24 -9.28
Endosome -9.78 11.96 6.02 15.18 -4.84 -1.74 6.25 4.33 5.25 -9.60 -9.36 13.38 5.81 15.43 -11.10
Golgi 2.29 5.98 7.06 5.38 2.47 -4.07 5.73 6.82 7.71 3.92 1.78 7.88 11.06 9.62 5.03
Lipid Particles 0.86 -1.12 -8.18 -8.34 -7.86 2.07 1.86 1.44 -1.29 -0.93 1.87 -1.74 -8.45 -9.33 -7.50
Mitochondria -1.03 -2.42 7.29 7.67 12.34 -1.02 -1.20 0.57 1.04 4.14 -1.64 -0.25 7.58 6.12 13.34
Mitotic Spindle 0.70 0.92 -1.38 -1.85 -2.07 -0.25 1.73 2.01 1.04 3.76 0.88 1.21 1.43 0.23 0.55
None 0.60 1.64 1.09 1.08 -3.09 -0.67 0.82 0.91 0.97 0.26 0.54 1.69 1.50 2.21 -2.47
Nuclear Periphery -3.10 1.82 -1.25 2.66 -6.18 -0.11 1.33 1.30 1.12 -0.65 -2.60 1.91 0.57 3.48 -5.77
Nuclear Periphery Foci -2.22 1.33 -2.53 0.27 -5.31 0.98 2.69 2.72 0.49 0.10 -1.93 1.31 -0.70 1.75 -3.64
Nucleolus -2.35 -0.20 -7.39 -1.21 -10.32 0.02 0.84 0.82 1.10 -0.99 -1.44 0.70 -0.93 1.07 -7.93
Nucleus -1.88 -0.73 -5.34 -4.94 -5.29 0.84 0.92 0.95 0.76 1.36 0.40 0.71 -1.84 -4.06 -4.85
Peroxisomes -1.29 -2.48 -6.63 -5.62 -5.14 0.20 2.66 1.99 1.84 -1.77 -0.85 -0.59 -4.66 -3.27 -4.24
Vacuole -9.62 -1.86 -10.26 -2.41 -9.65 -0.10 -3.96 -7.26 -3.56 -5.32 -9.63 -5.35 -14.71 -4.13 -11.62
Vacuole Periphery -10.97 2.11 6.18 16.71 5.33 -1.43 -1.13 -5.15 -1.19 -8.06 -11.38 2.16 -1.69 10.88 -4.86
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Gamma subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
Localization
Cell Percentages cytoplasm (91%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Gcd1

Gcd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gcd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available