Standard name
Human Ortholog
Description Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0.05 0.05 0 0 0
Bud 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 – – – 0.07 0.08 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.1 0.13 0.09 0.1 0 0
Bud Site 0.06 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.83 0.74 0.88 0.89 0.87 0.79 0.7 0.75 0.6 0.68 0.53 0.57 0.99 0.99 0.99 – – – 0.59 0.5 0.54 0.67 0.76 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.26 0.18 0 0 0.14 0.33 0.51 0.4 0.55 0.54 0.7 0.63 0 0 0 – – – 0.11 0.08 0.17 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0.07 0 0.08 0.07 0 0 0 0.05 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 8 2 4 1 0 0 0 1 0 0 1 3 1 1 – – – 3 5 21 9 11 10
Bud 2 0 10 7 8 11 23 11 13 20 15 15 1 1 0 – – – 7 9 12 10 9 17
Bud Neck 1 0 10 1 8 2 5 3 4 5 1 2 2 1 2 – – – 11 14 36 31 13 4
Bud Site 7 9 18 9 3 4 3 6 3 6 5 1 0 0 1 – – – – – – – – –
Cell Periphery 2 0 4 1 2 4 3 0 1 1 0 1 5 1 1 – – – 1 0 4 4 7 7
Cytoplasm 92 61 361 199 285 336 340 299 249 330 192 277 357 461 501 – – – 67 55 217 203 315 389
Endoplasmic Reticulum 0 1 0 0 2 1 0 0 0 0 0 2 0 0 0 – – – 0 2 6 0 2 0
Endosome 0 0 5 0 2 1 1 2 0 0 0 0 1 0 1 – – – 1 4 8 3 2 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – – – 0 0 2 0 0 2
Mitochondria 29 15 4 8 46 143 248 158 227 263 257 304 0 0 0 – – – 12 8 67 15 13 21
Nucleus 0 1 1 0 2 1 0 0 1 2 0 2 1 1 0 – – – 0 0 0 2 5 2
Nuclear Periphery 0 1 0 1 0 0 1 0 0 0 0 0 0 0 0 – – – 0 0 0 0 1 2
Nucleolus 0 1 1 0 1 0 2 1 2 1 0 2 0 0 0 – – – 0 0 1 1 1 0
Peroxisomes 3 5 6 6 4 5 0 0 0 2 0 0 0 0 1 – – – 0 0 1 0 0 1
SpindlePole 8 2 31 15 11 12 18 18 16 14 11 13 2 2 4 – – – 2 2 16 7 9 12
Vac/Vac Membrane 0 1 1 1 0 2 2 0 2 1 6 5 0 0 0 – – – 1 2 3 6 6 6
Unique Cell Count 111 82 409 224 329 427 487 398 414 487 365 484 362 464 508 114 111 407 305 413 495
Labelled Cell Count 145 105 454 252 375 522 646 498 519 645 487 625 372 468 513 114 111 407 305 413 495


Bud Neck

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.0 3.6 3.3 3.5 3.0 2.7 2.8 2.6 2.4 2.2 2.3 4.8 5.1 4.7 – – – 4.9 5.2 5.3
Std Deviation (1e-4) 0.5 0.8 1.4 1.3 1.5 1.6 1.1 1.3 1.0 0.9 0.6 0.7 0.9 1.6 1.1 – – – 1.0 1.2 1.5
Intensity Change (Log2) – – – -0.12 -0.04 -0.29 -0.43 -0.35 -0.47 -0.57 -0.69 -0.66 0.4 0.49 0.38 – – – 0.43 0.51 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0847 1.0958 1.0375 0.8169 0.4025 1.6031 0.2429 -0.7101 -0.9877 -0.5905 -0.1771 -0.0608 -0.8982 -1.2721 -1.4335 -1.3442 -1.4675 -0.8027
Actin 0.0129 0.0003 0.0233 0.0002 0.0002 0.0201 0.0716 0.0077 0.0167 0.0108 0.0005 0.0128 0.0317 0.0102 0.0007 0.0006 0.0165 0.0062
Bud 0.0025 0.014 0.0026 0.0001 0.0002 0.0003 0.0022 0.0024 0.0002 0.0004 0.0004 0.0019 0.002 0.0069 0.0049 0.0123 0.02 0.0018
Bud Neck 0.0683 0.0007 0.0004 0.0001 0 0.2504 0.0576 0.0013 0.0119 0.0001 0.0001 0.3986 0.0457 0.0025 0.0011 0.0005 0.037 0.3404
Bud Periphery 0.0107 0.0203 0.0106 0.0001 0.0004 0.0008 0.005 0.0053 0.0002 0.0003 0.0005 0.0009 0.005 0.0047 0.0041 0.0036 0.0097 0.001
Bud Site 0.0327 0.0384 0.0224 0 0 0.0012 0.0991 0.0747 0.0229 0.0003 0.0012 0.0033 0.0316 0.0455 0.0109 0.0214 0.0029 0.0032
Cell Periphery 0.0017 0.0009 0.0004 0 0.0001 0.0004 0.003 0.0008 0.0002 0 0.0002 0.0003 0.0013 0.001 0.0003 0.0003 0.0001 0.0003
Cytoplasm 0.0578 0.0353 0.0125 0.0143 0.0316 0.0539 0.0732 0.0834 0.0616 0.056 0.1602 0.0803 0.0559 0.0672 0.0348 0.018 0.041 0.0679
Cytoplasmic Foci 0.0084 0.0017 0.0036 0.0019 0.0048 0.0066 0.036 0.0177 0.005 0.011 0.0047 0.0272 0.0424 0.0049 0.0127 0.0272 0.0146 0.0134
Eisosomes 0.0009 0.0003 0.0003 0.0001 0.0001 0.0003 0.0015 0.0004 0.0001 0.0001 0 0.0001 0.0011 0.0002 0.0002 0.0001 0.0002 0.0003
Endoplasmic Reticulum 0.0034 0.0003 0.0009 0.0001 0.0007 0.001 0.0063 0.0008 0.0005 0.0024 0.0034 0.0011 0.0038 0.0004 0.0007 0.0004 0.0006 0.0003
Endosome 0.0157 0.0003 0.006 0.0001 0.0011 0.0029 0.036 0.0034 0.003 0.0226 0.0063 0.014 0.0103 0.0008 0.0006 0.0024 0.0046 0.0008
Golgi 0.0016 0.0001 0.0038 0 0.0001 0.0019 0.0205 0.002 0.0031 0.0111 0.0005 0.0076 0.0075 0.0002 0.0001 0.0007 0.0027 0.0009
Lipid Particles 0.0138 0.0001 0.0004 0.0001 0.0003 0.0012 0.0299 0.0039 0.0009 0.009 0.0003 0.0073 0.0086 0.0001 0.0004 0.0032 0.005 0.0034
Mitochondria 0.0051 0.0007 0.0034 0.0001 0.0003 0.0004 0.0162 0.0018 0.0008 0.0018 0.0009 0.0083 0.0017 0.0003 0.0019 0.0006 0.0026 0.0009
None 0.7395 0.8839 0.9062 0.9816 0.9579 0.6499 0.5028 0.7811 0.8705 0.8675 0.8132 0.4173 0.727 0.8495 0.907 0.8985 0.8026 0.5455
Nuclear Periphery 0.0088 0.0002 0.0002 0.0001 0.0004 0.0038 0.0168 0.0006 0.0001 0.0017 0.0015 0.0015 0.0035 0.0002 0.0009 0.0003 0.0021 0.0004
Nucleolus 0.0029 0.0004 0.0002 0.0001 0.0001 0.0008 0.0009 0.0006 0.0001 0.0001 0.0002 0.0005 0.0011 0.0005 0.0017 0.0006 0.0007 0.001
Nucleus 0.0027 0.0007 0.0002 0.0001 0.0003 0.0005 0.0033 0.0008 0.0002 0.0004 0.0028 0.0021 0.0018 0.0015 0.0036 0.0028 0.0015 0.0016
Peroxisomes 0.0018 0.0002 0.0014 0.0001 0.0001 0.0006 0.0073 0.0076 0.0007 0.002 0.0002 0.0107 0.0105 0.001 0.0004 0.0045 0.031 0.0064
Punctate Nuclear 0.0059 0.0004 0.0006 0.0008 0.0002 0.0027 0.0048 0.0028 0.0004 0.0015 0.0005 0.0033 0.0052 0.0015 0.0124 0.0016 0.0038 0.0037
Vacuole 0.002 0.0007 0.0004 0.0001 0.001 0.0002 0.0037 0.0007 0.0005 0.0005 0.0021 0.0006 0.0017 0.0009 0.0007 0.0005 0.0006 0.0004
Vacuole Periphery 0.001 0.0001 0.0002 0 0.0001 0.0001 0.0025 0.0001 0.0001 0.0003 0.0004 0.0004 0.0006 0.0001 0.0002 0.0001 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.9736 0.7629 22.0769 82.7626 35.4057 2.5587 2.5068 3.9044 42.3922 57.1199
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –
Standard name
Human Ortholog
Description Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress
Localization
Cell Percentages cytoplasm (10%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dse3

Dse3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dse3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available