ORF
Human Ortholog
Description Phosphatase with a broad substrate specificity; has some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.99 0.98 0.95 0.89 0.86 0.75 – 0.69 0.66 0.65 0.53 0.99 1.0 0.99 0.89 0.96 0.97 0.92 0.94 0.97 0.95 0.94 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.06 0 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0.05 – 0.06 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0.1 0.11 0.24 – 0.2 0.28 0.21 0.32 0 0 0 0.09 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 2 – 1 1 9 5 0 0 0 0 0 0 2 0 0 0 0 0
Bud 0 0 0 0 1 1 0 – 0 0 3 0 0 0 0 0 0 0 1 0 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 1 1 2 1 3 – 4 9 4 3 2 1 1 1 1 1 0 0 0 0 0 0
Cytoplasm 169 235 184 259 293 194 337 – 212 245 253 215 193 315 377 49 74 66 176 219 183 161 267 213
Endoplasmic Reticulum 1 1 1 6 10 3 16 – 10 13 17 15 2 2 2 2 0 2 0 0 0 1 3 5
Endosome 0 0 0 0 0 0 0 – 0 0 2 2 0 0 0 0 0 0 0 1 0 0 0 0
Golgi 1 1 0 0 0 1 0 – 1 5 2 4 0 0 1 0 0 0 0 0 0 0 1 0
Mitochondria 1 0 0 0 0 3 2 – 17 7 19 33 0 0 0 0 1 0 0 1 0 1 0 0
Nucleus 4 1 3 5 6 0 22 – 17 13 19 21 0 0 1 2 0 0 3 6 2 1 2 6
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 6 0 2 14 32 25 106 – 60 104 83 130 0 0 1 5 4 2 2 2 1 1 1 5
Unique Cell Count 178 237 188 272 328 226 450 306 372 387 403 194 316 380 55 77 68 192 235 189 170 283 238
Labelled Cell Count 183 238 191 285 344 229 488 322 398 411 429 197 318 383 60 80 71 192 235 189 170 283 238


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 22.5 23.6 20.7 21.3 21.3 13.5 15.2 – 12.1 11.8 11.5 11.1 24.5 24.7 23.9 22.3 23.9 23.9 24.2 23.5 23.9
Std Deviation (1e-4) 6.6 4.5 3.3 3.5 4.1 3.3 2.7 – 2.1 2.0 2.0 1.9 4.4 4.3 4.1 5.8 6.3 6.0 6.3 5.4 5.6
Intensity Change (Log2) – – – 0.04 0.04 -0.62 -0.45 – -0.77 -0.81 -0.85 -0.9 0.24 0.26 0.2 0.11 0.21 0.2 0.22 0.18 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.5 -3.5 -4.3 -6.8 – -7.7 -8.5 -8.6 -10.8 1.4 2.0 1.4 -2.9 -0.8 -0.4
Endoplasmic Reticulum 0 0 0 2.2 – 0 0 2.5 2.2 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 3.3 0 3.1 4.0 0 0 0 0 0 0
Nucleus 0 0 0 2.0 – 2.2 1.3 1.9 2.1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 2.3 3.8 4.1 6.9 – 6.0 7.7 6.5 8.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.1492 13.6615 12.4695 11.2676 9.7552 12.034 17.1288 21.6199 19.6091 18.9843 17.1285 20.4837 18.5035 23.1701 20.5692 20.6269 19.3347 20.0835
Actin 0.0068 0.0003 0.0002 0.0821 0.0135 0.0001 0.0009 0.0001 0.0078 0.0109 0.0247 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0003
Bud 0.0002 0.0003 0.0001 0.0001 0.0011 0.0001 0.0001 0.0001 0.0003 0.0005 0.0005 0.0001 0.0001 0.0001 0 0 0.0001 0.0001
Bud Neck 0.0008 0.0001 0.0002 0.005 0.001 0.0008 0.0002 0.0001 0.0003 0.0019 0.0046 0.0006 0.0002 0.0001 0.0002 0.0002 0.0003 0.0006
Bud Periphery 0.0002 0.0001 0 0.0001 0.0021 0 0.0001 0 0.0002 0.0005 0.0006 0 0 0 0 0 0 0
Bud Site 0.0009 0.0001 0.0001 0.0001 0.001 0 0.0002 0 0.0017 0.0002 0.0017 0 0.0001 0.0001 0 0 0 0
Cell Periphery 0.0001 0 0 0.0001 0.0001 0 0 0 0 0.0001 0.0001 0 0 0 0 0 0 0
Cytoplasm 0.8948 0.981 0.9588 0.8442 0.8288 0.9254 0.9118 0.9865 0.937 0.9552 0.8947 0.963 0.9482 0.9897 0.9672 0.977 0.941 0.9357
Cytoplasmic Foci 0.0071 0.0005 0.0014 0.0008 0.0235 0.0016 0.0167 0.0004 0.0105 0.0009 0.0055 0.0003 0.002 0.0002 0.0005 0.0003 0.0006 0.0006
Eisosomes 0.0001 0 0 0.0002 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0026 0.0009 0.0015 0.003 0.0026 0.0023 0.0017 0.0003 0.0012 0.0052 0.0018 0.0005 0.001 0.0001 0.0004 0.0001 0.0005 0.0007
Endosome 0.0103 0.0004 0.0027 0.0012 0.0245 0.0058 0.0192 0.0001 0.0098 0.0019 0.0073 0.0001 0.0002 0 0.0001 0.0001 0.0002 0.0002
Golgi 0.0022 0.0001 0.0001 0.0014 0.0139 0.0001 0.0035 0 0.0049 0.0007 0.0129 0 0 0 0 0 0 0
Lipid Particles 0.01 0 0.0002 0.0002 0.0095 0 0.0041 0 0.0048 0 0.001 0 0.0002 0 0 0 0 0
Mitochondria 0.0031 0.001 0.0001 0.0002 0.0241 0.0002 0.0011 0 0.005 0.0006 0.003 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0002
None 0.0027 0.0004 0.0007 0.0004 0.0043 0.0005 0.0013 0.0005 0.0017 0.0003 0.0004 0.0003 0.001 0.0003 0.0004 0.0002 0.0005 0.0004
Nuclear Periphery 0.0068 0.0019 0.0043 0.0047 0.0056 0.0059 0.0131 0.0007 0.0017 0.0016 0.0115 0.0022 0.0044 0.0005 0.002 0.0009 0.0034 0.0028
Nucleolus 0.0009 0 0 0 0.0002 0 0.0002 0 0.0001 0 0.0002 0 0 0 0 0 0 0
Nucleus 0.0419 0.0123 0.028 0.0547 0.0246 0.0537 0.0195 0.011 0.008 0.0188 0.0201 0.0323 0.0345 0.0086 0.0284 0.0209 0.052 0.0576
Peroxisomes 0.0029 0 0 0.0002 0.0115 0 0.002 0 0.0025 0 0.002 0 0 0 0 0 0 0
Punctate Nuclear 0.0027 0.0002 0.0005 0.0007 0.0036 0.0006 0.0026 0.0001 0.0016 0.0001 0.0052 0.0002 0.0073 0.0001 0.0002 0.0001 0.0009 0.0004
Vacuole 0.0021 0.0004 0.0008 0.0003 0.0025 0.0023 0.0008 0.0001 0.0005 0.0004 0.0015 0.0002 0.0002 0 0.0001 0.0001 0.0002 0.0002
Vacuole Periphery 0.0006 0.0001 0.0003 0.0001 0.0019 0.0004 0.0011 0 0.0004 0.0001 0.0005 0 0.0001 0 0 0 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.3781 37.0069 32.7416 36.2623 33.03 35.0093 34.5394 26.2257 29.4073 41.3092
Translational Efficiency 2.3674 2.5558 2.3293 1.8631 2.4466 2.3786 2.2444 2.3009 2.2131 2.2265

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2266 857 166 1574 956 2411 173 1577 3222 3268 339 3151

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1667.07 1809.61 2342.77 2110.38 1510.60 1858.51 2351.49 2124.78 1620.64 1845.69 2347.22 2117.59
Standard Deviation 281.88 326.02 374.61 385.59 288.92 309.13 364.38 459.12 292.84 314.38 369.45 424.05
Intensity Change Log 2 — 0.118364 0.490901 0.340188 — 0.299025 0.638454 0.492192 — 0.207072 0.562928 0.414447

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000106 0.000198 0.000796 0.000970 0.000383 0.000128 0.000897 0.000622 0.000188 0.000146 0.000847 0.000796
Bud Neck 0.000392 0.000544 0.003470 0.004865 0.000452 0.000542 0.004964 0.002142 0.000409 0.000542 0.004233 0.003502
Bud Site 0.001229 0.001666 0.003605 0.023058 0.001679 0.002188 0.007845 0.007117 0.001362 0.002051 0.005769 0.015080
Cell Periphery 0.000045 0.000129 0.000738 0.000186 0.000132 0.000032 0.001036 0.000137 0.000071 0.000057 0.000890 0.000161
Cytoplasm 0.793654* 0.733617* 0.372949* 0.630273* 0.766066* 0.764557* 0.526013* 0.740757* 0.785468* 0.756444* 0.451061* 0.685568*
Cytoplasmic Foci 0.005408 0.007544 0.016061 0.012840 0.007256 0.004163 0.026757 0.004541 0.005956 0.005049 0.021519 0.008687
Eisosomes 0.000001 0.000001 0.000006 0.000007 0.000003 0.000001 0.000009 0.000004 0.000002 0.000001 0.000008 0.000006
Endoplasmic Reticulum 0.000189 0.000126 0.000571 0.000390 0.000708 0.000070 0.000339 0.000590 0.000343 0.000085 0.000453 0.000490
Endosome 0.000087 0.000266 0.000554 0.010469 0.000401 0.000212 0.000631 0.000922 0.000180 0.000226 0.000593 0.005691
Golgi 0.000186 0.000537 0.001218 0.009922 0.000899 0.000375 0.000792 0.000733 0.000397 0.000417 0.001001 0.005323
Lipid Particles 0.000154 0.000278 0.001487 0.002324 0.000384 0.000116 0.002748 0.000599 0.000222 0.000159 0.002131 0.001461
Mitochondria 0.000109 0.000107 0.000424 0.001327 0.000802 0.000221 0.000189 0.000230 0.000315 0.000191 0.000304 0.000778
Mitotic Spindle 0.002253 0.002051 0.001561 0.081649 0.000587 0.004505 0.001699 0.008477 0.001758 0.003861 0.001632 0.045028
None 0.010057 0.020032 0.006065 0.009453 0.008077 0.017610 0.010002 0.015630 0.009470 0.018245 0.008074 0.012544
Nuclear Periphery 0.000077 0.000064 0.000573 0.001976 0.000100 0.000065 0.000566 0.000150 0.000084 0.000064 0.000570 0.001062
Nuclear Periphery Foci 0.001159 0.001802 0.002388 0.007149 0.003911 0.000591 0.007964 0.002334 0.001975 0.000908 0.005234 0.004739
Nucleolus 0.000105 0.000096 0.000139 0.000485 0.000130 0.000119 0.000180 0.000131 0.000113 0.000113 0.000160 0.000308
Nucleus 0.179928* 0.225129* 0.566955* 0.152511* 0.198241* 0.200798* 0.353926* 0.202978* 0.185361* 0.207179* 0.458241* 0.177769*
Peroxisomes 0.000025 0.000025 0.000058 0.000562 0.000048 0.000146 0.000083 0.000074 0.000032 0.000115 0.000071 0.000317
Vacuole 0.004501 0.005613 0.020204 0.040078 0.008788 0.003444 0.053239 0.009615 0.005773 0.004012 0.037063 0.024832
Vacuole Periphery 0.000335 0.000176 0.000177 0.009505 0.000952 0.000117 0.000121 0.002217 0.000518 0.000133 0.000149 0.005858

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.04 -7.68 -3.37 -1.32 4.88 4.14 -6.12 0.09 -2.31 5.00 0.90 -10.72 -2.79 -3.13 7.00
Bud Neck -3.97 -7.65 -8.32 -6.61 3.90 -0.52 -10.59 -5.97 -5.49 8.45 -2.38 -12.90 -10.25 -8.94 8.70
Bud Site -2.01 -5.73 -6.14 -5.22 -1.14 -0.31 -7.58 -4.07 -3.58 4.87 -1.36 -9.28 -7.44 -6.04 3.20
Cell Periphery -6.38 -10.09 -12.25 -0.48 8.77 8.54 -10.51 -4.32 -12.48 9.83 -0.23 -14.82 -13.19 -11.82 13.17
Cytoplasm 10.43 28.79 23.64 9.84 -17.74 0.83 20.82 7.98 9.36 -17.49 9.08 35.37 24.25 15.99 -24.49
Cytoplasmic Foci -3.94 -7.09 -5.77 0.77 5.69 4.00 -8.43 -1.86 -6.41 7.98 0.25 -11.12 -6.03 -5.46 9.71
Eisosomes -4.50 -6.78 -13.80 -7.52 4.28 3.81 -9.85 -0.96 -4.34 9.17 1.10 -12.11 -7.91 -8.84 9.35
Endoplasmic Reticulum 0.90 -2.51 1.31 0.16 2.74 4.64 1.73 2.76 -0.85 1.91 5.48 -2.04 2.21 -1.09 3.36
Endosome -2.26 -5.03 -3.72 -3.23 -2.10 1.90 -3.82 0.48 -0.95 3.73 -0.69 -7.53 -3.66 -3.45 -0.71
Golgi -1.83 -4.64 -2.87 -2.05 -0.47 1.42 -0.08 1.51 0.18 4.21 0.05 -4.32 -2.01 -2.05 0.61
Lipid Particles -4.34 -8.17 -9.66 -7.19 2.94 2.92 -8.50 -5.78 -8.25 6.84 -0.57 -11.56 -11.83 -11.65 7.21
Mitochondria 0.14 -8.93 -3.02 -3.00 2.48 1.19 1.07 1.33 0.92 3.59 0.84 -0.84 0.39 -1.08 3.42
Mitotic Spindle 0.59 -0.56 -6.12 -6.29 -5.74 -3.69 -3.81 -3.86 -0.68 -2.17 -2.00 -1.35 -6.74 -5.22 -6.11
None -5.10 7.49 5.74 7.32 -2.69 -6.89 1.29 -2.29 7.26 -5.11 -9.73 5.83 1.28 11.84 -5.70
Nuclear Periphery -1.80 -9.26 -6.53 -6.29 2.45 2.01 -14.18 -8.66 -10.38 11.74 -0.79 -15.51 -7.96 -7.90 7.19
Nuclear Periphery Foci -3.33 -6.10 -3.83 -1.14 1.62 5.76 -4.10 3.34 -3.50 6.51 4.21 -7.11 -0.96 -4.44 6.36
Nucleolus 0.93 -2.51 -3.65 -4.63 -2.21 0.03 -1.90 0.03 -0.01 4.54 0.05 -3.42 -2.86 -2.66 0.05
Nucleus -7.25 -19.68 1.49 7.84 19.90 -1.27 -7.22 -0.42 1.02 7.25 -6.96 -17.83 -0.48 6.35 17.65
Peroxisomes -0.37 -5.24 -3.56 -3.36 -0.61 -1.84 -3.97 0.13 1.94 5.38 -2.19 -7.58 -3.51 0.73 1.74
Vacuole -5.17 -7.86 -27.82 -23.62 -8.76 3.26 -11.56 -15.66 -17.72 8.05 -2.97 -13.08 -31.76 -30.15 2.46
Vacuole Periphery 0.57 0.35 -2.02 -2.62 -2.52 1.31 1.21 0.40 -1.13 -1.00 1.41 1.14 -1.27 -2.76 -2.54
ORF
Human Ortholog
Description Phosphatase with a broad substrate specificity; has some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

YOR283W

YOR283W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yor283w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available