Standard name | |
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Human Ortholog | |
Description | Subunit of UAF (upstream activation factor) complex; UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; targeting factor for the UAF that facilitates activation of many rDNA genes; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 2 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 2 | 0 | 1 | 3 | 3 | 7 | 6 | – | 9 | 12 | 22 | 15 | 0 | 0 | 1 | 2 | 0 | 0 | 3 | 4 | 0 | 1 | 2 | 1 |
Bud Neck | 7 | 0 | 8 | 16 | 42 | 18 | 24 | – | 27 | 29 | 19 | 26 | 8 | 7 | 11 | 0 | 1 | 3 | 2 | 2 | 1 | 3 | 1 | 6 |
Bud Site | 4 | 1 | 0 | 6 | 0 | 8 | 7 | – | 7 | 27 | 27 | 25 | 1 | 1 | 0 | 3 | 0 | 3 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 7 | 3 | 14 | 28 | 16 | 39 | – | 12 | 29 | 19 | 22 | 16 | 46 | 54 | 70 | 39 | 43 | 0 | 3 | 0 | 2 | 3 | 5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 6 | 3 | 4 | 0 | 0 | 0 | 0 | 2 | 0 |
Endosome | 35 | 35 | 1 | 8 | 14 | 0 | 10 | – | 3 | 3 | 5 | 14 | 37 | 57 | 45 | 23 | 20 | 17 | 36 | 21 | 15 | 18 | 29 | 27 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 2 | 6 | 1 |
Mitochondria | 41 | 40 | 3 | 3 | 4 | 27 | 21 | – | 48 | 72 | 85 | 81 | 4 | 5 | 3 | 19 | 12 | 15 | 5 | 2 | 0 | 1 | 2 | 3 |
Nucleus | 29 | 37 | 15 | 28 | 24 | 10 | 32 | – | 15 | 25 | 26 | 17 | 25 | 24 | 18 | 22 | 16 | 21 | 7 | 18 | 4 | 2 | 3 | 2 |
Nuclear Periphery | 2 | 1 | 2 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 4 | 0 | 1 | 3 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 93 | 54 | 16 | 20 | 22 | 13 | 19 | – | 33 | 46 | 35 | 55 | 72 | 95 | 61 | 34 | 29 | 43 | 60 | 91 | 45 | 38 | 30 | 21 |
Peroxisomes | 4 | 6 | 0 | 4 | 0 | 0 | 1 | – | 2 | 2 | 0 | 8 | 2 | 10 | 11 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 |
SpindlePole | 66 | 81 | 82 | 118 | 222 | 60 | 180 | – | 136 | 193 | 176 | 236 | 105 | 164 | 108 | 53 | 51 | 50 | 87 | 71 | 60 | 87 | 134 | 112 |
Vac/Vac Membrane | 2 | 5 | 1 | 1 | 2 | 1 | 1 | – | 2 | 1 | 2 | 1 | 1 | 1 | 0 | 7 | 7 | 11 | 4 | 10 | 1 | 3 | 4 | 5 |
Unique Cell Count | 189 | 184 | 103 | 152 | 290 | 116 | 261 | 210 | 293 | 280 | 347 | 181 | 277 | 217 | 160 | 115 | 136 | 222 | 238 | 138 | 172 | 226 | 195 | |
Labelled Cell Count | 289 | 267 | 132 | 223 | 363 | 160 | 340 | 295 | 439 | 417 | 500 | 276 | 410 | 313 | 243 | 179 | 212 | 222 | 238 | 138 | 172 | 226 | 195 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.5 | 5.0 | 4.9 | 5.0 | 5.6 | 3.7 | 4.7 | – | 4.1 | 4.0 | 3.8 | 4.0 | 5.2 | 5.4 | 5.4 | 7.1 | 6.8 | 7.0 | 4.9 | 5.1 | 5.1 |
Std Deviation (1e-4) | 1.0 | 0.9 | 0.9 | 1.5 | 1.4 | 1.3 | 1.8 | – | 1.3 | 1.2 | 1.0 | 1.1 | 0.9 | 1.6 | 1.7 | 2.1 | 1.8 | 1.8 | 1.7 | 1.6 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.19 | -0.41 | -0.06 | – | -0.27 | -0.29 | -0.36 | -0.29 | 0.1 | 0.14 | 0.15 | 0.54 | 0.48 | 0.53 | -0.0 | 0.06 | 0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.7 | 1.8 | 0 | 0 | – | 0 | 0 | 0 | 0 | -1.2 | 0 | -1.0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.0 | 2.2 | 2.9 | 3.2 | – | 0 | 2.2 | 1.4 | 1.3 | 1.9 | 3.5 | 4.8 | 7.2 | 5.8 | 5.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 4.6 | 4.7 | 4.7 | 3.7 | 4.1 | 3.3 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 2.2 | 2.4 |
Nucleus | 0.8 | -1.8 | -1.4 | -0.6 | – | -2.1 | -1.7 | -1.5 | -3.4 | -0.2 | -1.7 | -1.7 | -0.2 | -0.1 | 0.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.5 | -2.3 | 0 | 0 | – | 0 | 0 | 0 | 0 | 4.2 | 3.6 | 2.5 | 1.2 | 1.8 | 2.9 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -0.4 | -0.6 | 0 | 0 | – | 0 | 0 | 0 | 0 | -3.7 | -3.7 | -5.1 | -7.4 | -5.3 | -6.6 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.8113 | -0.5327 | -0.3854 | -0.9445 | -1.0488 | -0.6889 | 1.4785 | 1.1263 | 0.8426 | 1.2412 | 0.4554 | 0.9538 | 1.8625 | 2.1981 | 1.8041 | 1.9589 | 1.6934 | 1.7755 |
Actin | 0.0368 | 0.0003 | 0.0124 | 0.004 | 0.0049 | 0.0009 | 0.0253 | 0.0027 | 0.0142 | 0.0212 | 0.0018 | 0.0009 | 0.0079 | 0.0001 | 0.0018 | 0.0001 | 0.0007 | 0.0002 |
Bud | 0.0003 | 0.0003 | 0.0002 | 0.0005 | 0.0004 | 0.0001 | 0.0022 | 0.0006 | 0.0005 | 0.0008 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Bud Neck | 0.0039 | 0.0076 | 0.0077 | 0.0045 | 0.0083 | 0.0041 | 0.0063 | 0.0076 | 0.0096 | 0.0212 | 0.0855 | 0.0145 | 0.0094 | 0.004 | 0.0064 | 0.0215 | 0.0054 | 0.0086 |
Bud Periphery | 0.0003 | 0.0006 | 0.0003 | 0.0007 | 0.0006 | 0.0001 | 0.0054 | 0.0008 | 0.0007 | 0.001 | 0.001 | 0.0005 | 0.0006 | 0.0002 | 0.0002 | 0.0004 | 0.0006 | 0.0003 |
Bud Site | 0.0023 | 0.0051 | 0.0025 | 0.0017 | 0.0154 | 0.0005 | 0.0037 | 0.0151 | 0.0037 | 0.0113 | 0.0051 | 0.0021 | 0.0057 | 0.0015 | 0.0078 | 0.0024 | 0.0006 | 0.0008 |
Cell Periphery | 0.0003 | 0.0008 | 0.0005 | 0.0003 | 0.0003 | 0.0001 | 0.0006 | 0.0006 | 0.0006 | 0.001 | 0.0003 | 0.0003 | 0.0007 | 0.0001 | 0.0005 | 0.0008 | 0.0002 | 0.0003 |
Cytoplasm | 0.0028 | 0.0039 | 0.0028 | 0.0332 | 0.0008 | 0.0014 | 0.01 | 0.0029 | 0.0084 | 0.0127 | 0.0099 | 0.0014 | 0.0039 | 0.0002 | 0.0008 | 0.0012 | 0.0003 | 0.001 |
Cytoplasmic Foci | 0.0945 | 0.0579 | 0.05 | 0.0371 | 0.0537 | 0.0366 | 0.0496 | 0.0372 | 0.0375 | 0.0342 | 0.0189 | 0.0221 | 0.0535 | 0.0153 | 0.0268 | 0.0424 | 0.0278 | 0.0233 |
Eisosomes | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0009 | 0.0007 | 0.0011 | 0.0013 | 0.0003 | 0.0001 | 0.0016 | 0.0021 | 0.0009 | 0.006 | 0.0023 | 0.0006 | 0.0008 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0054 |
Endosome | 0.0223 | 0.0197 | 0.0245 | 0.0361 | 0.0096 | 0.0052 | 0.0549 | 0.0196 | 0.0184 | 0.0328 | 0.0172 | 0.0184 | 0.0162 | 0.0017 | 0.0106 | 0.0077 | 0.0055 | 0.0041 |
Golgi | 0.0106 | 0.0052 | 0.02 | 0.0135 | 0.0073 | 0.0037 | 0.0333 | 0.0091 | 0.0109 | 0.0298 | 0.0091 | 0.0052 | 0.007 | 0.0014 | 0.0033 | 0.0025 | 0.0051 | 0.0025 |
Lipid Particles | 0.2063 | 0.1714 | 0.1615 | 0.0843 | 0.1665 | 0.1145 | 0.1139 | 0.081 | 0.0888 | 0.1021 | 0.0598 | 0.0686 | 0.1564 | 0.0383 | 0.089 | 0.0847 | 0.1661 | 0.0717 |
Mitochondria | 0.0016 | 0.0013 | 0.0194 | 0.0582 | 0.0034 | 0.0002 | 0.0389 | 0.0054 | 0.0031 | 0.0073 | 0.027 | 0.0007 | 0.0055 | 0.0002 | 0.0004 | 0.0012 | 0.0094 | 0.0003 |
None | 0.0042 | 0.0133 | 0.0258 | 0.0232 | 0.0034 | 0.0009 | 0.038 | 0.0132 | 0.0024 | 0.0008 | 0.0046 | 0.0005 | 0.0164 | 0.0021 | 0.0015 | 0.0027 | 0.0009 | 0.0148 |
Nuclear Periphery | 0.003 | 0.0035 | 0.013 | 0.0031 | 0.0022 | 0.004 | 0.0052 | 0.0022 | 0.0078 | 0.0036 | 0.0129 | 0.0063 | 0.0053 | 0.0019 | 0.0017 | 0.0004 | 0.0042 | 0.0025 |
Nucleolus | 0.2123 | 0.3674 | 0.3702 | 0.4699 | 0.0894 | 0.2048 | 0.2101 | 0.4809 | 0.5185 | 0.489 | 0.2583 | 0.3255 | 0.2358 | 0.4321 | 0.4809 | 0.4515 | 0.2416 | 0.2111 |
Nucleus | 0.0188 | 0.0353 | 0.0256 | 0.0358 | 0.0075 | 0.028 | 0.0505 | 0.0752 | 0.073 | 0.0399 | 0.0738 | 0.0283 | 0.0365 | 0.034 | 0.0443 | 0.0295 | 0.027 | 0.0209 |
Peroxisomes | 0.0289 | 0.0074 | 0.0149 | 0.0056 | 0.0434 | 0.0099 | 0.0191 | 0.0071 | 0.0044 | 0.0048 | 0.0042 | 0.0065 | 0.0201 | 0.0027 | 0.0082 | 0.017 | 0.0472 | 0.0058 |
Punctate Nuclear | 0.3468 | 0.2868 | 0.2431 | 0.1778 | 0.5802 | 0.5838 | 0.3134 | 0.2247 | 0.1858 | 0.1677 | 0.4017 | 0.4937 | 0.4136 | 0.4632 | 0.3104 | 0.3286 | 0.455 | 0.6245 |
Vacuole | 0.0025 | 0.0103 | 0.0033 | 0.0054 | 0.0018 | 0.0008 | 0.0135 | 0.0109 | 0.0096 | 0.0117 | 0.0042 | 0.0031 | 0.0035 | 0.0007 | 0.0045 | 0.0046 | 0.0013 | 0.0016 |
Vacuole Periphery | 0.0005 | 0.0009 | 0.001 | 0.0037 | 0.0005 | 0.0002 | 0.0041 | 0.001 | 0.0013 | 0.0012 | 0.0017 | 0.0005 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0006 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.6214 | 4.3073 | 2.1308 | 9.3071 | 10.412 | 3.2105 | 9.4361 | 6.3687 | 10.4847 | 9.3928 |
Translational Efficiency | 1.7034 | 2.3437 | 3.8698 | 1.0842 | 0.8862 | 3.1214 | 1.4302 | 1.5247 | 0.773 | 1.0627 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2058 | 949 | 1992 | 1622 | 1896 | 2180 | 157 | 1837 | 3954 | 3129 | 2149 | 3459 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 610.03 | 763.11 | 848.24 | 873.96 | 679.15 | 761.74 | 973.97 | 886.17 | 643.17 | 762.16 | 857.43 | 880.44 |
Standard Deviation | 77.19 | 99.19 | 102.24 | 119.84 | 81.69 | 98.89 | 111.67 | 117.79 | 86.57 | 98.98 | 108.04 | 118.91 |
Intensity Change Log 2 | — | 0.323011 | 0.475592 | 0.518687 | — | 0.165568 | 0.520147 | 0.383853 | — | 0.242214 | 0.499236 | 0.449228 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000209 | 0.000711 | 0.000404 | 0.000495 | 0.000179 | 0.000335 | 0.001118 | 0.000661 | 0.000195 | 0.000449 | 0.000456 | 0.000583 |
Bud Neck | 0.008495 | 0.015077 | 0.012819 | 0.026892 | 0.006081 | 0.018911 | 0.011676 | 0.027290 | 0.007337 | 0.017748 | 0.012736 | 0.027103 |
Bud Site | 0.002064 | 0.006775 | 0.002885 | 0.017390 | 0.001266 | 0.006218 | 0.003684 | 0.019057 | 0.001681 | 0.006387 | 0.002944 | 0.018275 |
Cell Periphery | 0.000304 | 0.000153 | 0.000055 | 0.000130 | 0.000121 | 0.000216 | 0.000092 | 0.000475 | 0.000217 | 0.000197 | 0.000058 | 0.000313 |
Cytoplasm | 0.035197 | 0.030336 | 0.007077 | 0.040778 | 0.031800 | 0.069782 | 0.003191 | 0.036338 | 0.033568 | 0.057819 | 0.006793 | 0.038420 |
Cytoplasmic Foci | 0.135295 | 0.066101 | 0.001281 | 0.003097 | 0.057845 | 0.054509 | 0.001713 | 0.007300 | 0.098157 | 0.058025 | 0.001313 | 0.005329 |
Eisosomes | 0.000166 | 0.000133 | 0.000056 | 0.000066 | 0.000128 | 0.000116 | 0.000167 | 0.000066 | 0.000148 | 0.000121 | 0.000064 | 0.000066 |
Endoplasmic Reticulum | 0.002038 | 0.003087 | 0.001986 | 0.002701 | 0.001111 | 0.001516 | 0.006294 | 0.002449 | 0.001593 | 0.001992 | 0.002301 | 0.002567 |
Endosome | 0.016092 | 0.033398 | 0.002685 | 0.009632 | 0.018431 | 0.026939 | 0.004277 | 0.013736 | 0.017214 | 0.028898 | 0.002802 | 0.011811 |
Golgi | 0.023973 | 0.014775 | 0.001123 | 0.002842 | 0.006449 | 0.009404 | 0.001612 | 0.004674 | 0.015570 | 0.011033 | 0.001158 | 0.003815 |
Lipid Particles | 0.143979* | 0.022258 | 0.000692 | 0.001788 | 0.044094 | 0.017631 | 0.002671 | 0.001322 | 0.096083 | 0.019034 | 0.000837 | 0.001541 |
Mitochondria | 0.035730 | 0.013725 | 0.001560 | 0.004171 | 0.007028 | 0.018740 | 0.001038 | 0.004715 | 0.021967 | 0.017219 | 0.001522 | 0.004460 |
Mitotic Spindle | 0.056894 | 0.346075* | 0.245007* | 0.483668* | 0.061113 | 0.104845* | 0.148277* | 0.459866* | 0.058917 | 0.178008* | 0.237941* | 0.471027* |
None | 0.029451 | 0.009655 | 0.002804 | 0.003575 | 0.014175 | 0.026876 | 0.002665 | 0.005094 | 0.022125 | 0.021653 | 0.002794 | 0.004382 |
Nuclear Periphery | 0.004330 | 0.002386 | 0.001797 | 0.005719 | 0.002741 | 0.002352 | 0.002918 | 0.002517 | 0.003568 | 0.002362 | 0.001879 | 0.004019 |
Nuclear Periphery Foci | 0.074890 | 0.095210 | 0.037543 | 0.040134 | 0.103084 | 0.033957 | 0.065689 | 0.021524 | 0.088409 | 0.052535 | 0.039600 | 0.030251 |
Nucleolus | 0.338435* | 0.222716* | 0.489466* | 0.236975* | 0.537451* | 0.417304* | 0.456903* | 0.263017* | 0.433866* | 0.358287* | 0.487087* | 0.250805* |
Nucleus | 0.067491 | 0.080674 | 0.186185* | 0.097692 | 0.091349 | 0.163442* | 0.268318* | 0.102612 | 0.078931 | 0.138339* | 0.192186* | 0.100305 |
Peroxisomes | 0.021485 | 0.024483 | 0.001908 | 0.002926 | 0.013126 | 0.016571 | 0.009000 | 0.004233 | 0.017477 | 0.018971 | 0.002426 | 0.003620 |
Vacuole | 0.002146 | 0.009032 | 0.001801 | 0.015577 | 0.001647 | 0.008159 | 0.008163 | 0.017775 | 0.001907 | 0.008424 | 0.002266 | 0.016744 |
Vacuole Periphery | 0.001336 | 0.003241 | 0.000863 | 0.003753 | 0.000782 | 0.002176 | 0.000534 | 0.005280 | 0.001070 | 0.002499 | 0.000839 | 0.004564 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -9.54 | -9.48 | -10.38 | 1.92 | -2.53 | -3.28 | -7.98 | -7.15 | -3.90 | 5.19 | -6.93 | -13.61 | -11.12 | -3.37 | -0.75 |
Bud Neck | -5.99 | -10.21 | -18.42 | -11.36 | -11.44 | -14.08 | -5.28 | -21.21 | -10.62 | -6.92 | -14.47 | -12.97 | -27.91 | -15.19 | -14.95 |
Bud Site | -6.96 | -6.99 | -12.84 | -8.54 | -10.36 | -6.52 | -4.40 | -13.36 | -9.62 | -3.80 | -8.58 | -9.29 | -18.46 | -12.92 | -13.83 |
Cell Periphery | 5.21 | 9.97 | 7.57 | 4.46 | -2.76 | -6.27 | -2.28 | -0.70 | 0.06 | -0.32 | 1.19 | 10.92 | 0.99 | 0.69 | -1.35 |
Cytoplasm | 0.72 | 11.50 | -1.67 | -2.01 | -10.05 | -8.95 | 7.85 | -1.81 | 6.97 | -9.04 | -7.70 | 13.11 | -2.33 | 5.19 | -13.18 |
Cytoplasmic Foci | 13.05 | 32.58 | 31.72 | 14.48 | -6.47 | 1.28 | 19.65 | 19.04 | 20.14 | -2.70 | 11.88 | 37.89 | 36.05 | 25.11 | -8.66 |
Eisosomes | 4.58 | 31.65 | 23.69 | 12.72 | -3.64 | 4.06 | -4.24 | 17.05 | 12.87 | 7.26 | 8.17 | 25.90 | 29.07 | 18.03 | 0.06 |
Endoplasmic Reticulum | -3.88 | -1.93 | -4.16 | -0.42 | -4.72 | -3.56 | -8.83 | -11.55 | -8.51 | 6.66 | -2.75 | -6.96 | -8.05 | -8.44 | -1.21 |
Endosome | -6.75 | 13.28 | 3.19 | 8.40 | -12.31 | -4.31 | 4.72 | 2.51 | 7.21 | -3.20 | -7.64 | 15.63 | 3.88 | 10.87 | -16.03 |
Golgi | 4.63 | 15.77 | 14.85 | 10.51 | -1.05 | -2.41 | 8.08 | 6.35 | 7.44 | -0.26 | 4.74 | 18.07 | 15.87 | 12.35 | -2.49 |
Lipid Particles | 23.56* | 30.17* | 29.95 | 9.96 | -5.95 | 8.21 | 14.54 | 16.26 | 12.30 | 3.69 | 24.55 | 32.83 | 32.58 | 15.74 | -4.87 |
Mitochondria | 9.17 | 18.29 | 17.55 | 6.55 | -3.55 | -6.66 | 7.06 | 3.87 | 9.23 | -4.64 | 3.48 | 18.92 | 17.23 | 11.27 | -4.95 |
Mitotic Spindle | -23.35* | -27.32* | -40.31* | -8.69 | -15.05 | -8.00 | -5.44 | -38.36* | -31.66* | -9.57 | -20.87 | -28.02 | -55.72 | -32.09* | -18.00 |
None | 10.19 | 16.34 | 15.93 | 6.44 | -2.06 | -5.22 | 9.32 | 8.60 | 12.99 | -2.11 | 1.58 | 19.16 | 17.79 | 14.52 | -3.77 |
Nuclear Periphery | 3.94 | 4.43 | -1.50 | -4.99 | -5.38 | 0.97 | -0.80 | -2.89 | -3.93 | -0.47 | 3.98 | 4.13 | -2.28 | -6.24 | -6.38 |
Nuclear Periphery Foci | -2.38 | 10.36 | 12.72 | 11.01 | 3.07 | 13.93* | 1.77 | 18.39 | 5.59 | 5.62 | 10.27 | 14.48 | 22.31 | 10.86 | 6.53 |
Nucleolus | 9.56 | -12.52 | 11.01 | 0.32 | 23.44* | 9.87 | 6.75 | 25.01* | 15.43 | 4.53 | 7.75 | -2.64 | 24.34 | 15.10 | 24.38* |
Nucleus | -3.63 | -20.19 | -8.26 | -3.68 | 10.23 | -13.11 | -8.04 | -6.19 | 6.47 | 6.38 | -14.68 | -21.13 | -10.29 | 4.73 | 12.39 |
Peroxisomes | -1.78 | 23.24 | 20.30 | 12.77 | -4.36 | -2.64 | 2.69 | 12.62 | 12.76 | 4.45 | -1.52 | 26.82 | 23.51 | 18.03 | -4.32 |
Vacuole | -4.83 | -2.78 | -11.88 | -9.47 | -11.41 | -7.00 | -3.65 | -11.41 | -8.54 | 0.73 | -8.25 | -4.94 | -16.32 | -12.89 | -13.40 |
Vacuole Periphery | -2.28 | 2.05 | -0.84 | 1.34 | -1.80 | -4.72 | -1.09 | -3.17 | -0.90 | -2.93 | -4.44 | 1.08 | -2.87 | 0.26 | -3.31 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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