Standard name
Human Ortholog
Description Subunit of UAF (upstream activation factor) complex; UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; targeting factor for the UAF that facilitates activation of many rDNA genes; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.06 0 – 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.08 0.11 0.14 0.16 0.09 – 0.13 0.1 0.07 0.07 0 0 0.05 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.07 0 – 0 0.09 0.1 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.09 0.1 0.14 0.15 – 0.06 0.1 0.07 0.06 0.09 0.17 0.25 0.44 0.34 0.32 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.19 0.19 0 0.05 0.05 0 0 – 0 0 0 0 0.2 0.21 0.21 0.14 0.17 0.12 0.16 0.09 0.11 0.11 0.13 0.14
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.22 0.22 0 0 0 0.23 0.08 – 0.23 0.25 0.3 0.23 0 0 0 0.12 0.1 0.11 0 0 0 0 0 0
Nucleus 0.15 0.2 0.15 0.18 0.08 0.09 0.12 – 0.07 0.09 0.09 0.05 0.14 0.09 0.08 0.14 0.14 0.15 0 0.08 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.49 0.29 0.16 0.13 0.08 0.11 0.07 – 0.16 0.16 0.12 0.16 0.4 0.34 0.28 0.21 0.25 0.32 0.27 0.38 0.33 0.22 0.13 0.11
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
SpindlePole 0.35 0.44 0.8 0.78 0.77 0.52 0.69 – 0.65 0.66 0.63 0.68 0.58 0.59 0.5 0.33 0.44 0.37 0.4 0.3 0.44 0.51 0.6 0.58
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 2 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 2 0 1 3 3 7 6 – 9 12 22 15 0 0 1 2 0 0 3 4 0 1 2 1
Bud Neck 7 0 8 16 42 18 24 – 27 29 19 26 8 7 11 0 1 3 2 2 1 3 1 6
Bud Site 4 1 0 6 0 8 7 – 7 27 27 25 1 1 0 3 0 3 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 7 3 14 28 16 39 – 12 29 19 22 16 46 54 70 39 43 0 3 0 2 3 5
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 6 3 4 0 0 0 0 2 0
Endosome 35 35 1 8 14 0 10 – 3 3 5 14 37 57 45 23 20 17 36 21 15 18 29 27
Golgi 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 4 0 0 2 6 1
Mitochondria 41 40 3 3 4 27 21 – 48 72 85 81 4 5 3 19 12 15 5 2 0 1 2 3
Nucleus 29 37 15 28 24 10 32 – 15 25 26 17 25 24 18 22 16 21 7 18 4 2 3 2
Nuclear Periphery 2 1 2 0 0 0 0 – 1 0 0 0 4 0 1 3 1 0 3 0 0 0 0 0
Nucleolus 93 54 16 20 22 13 19 – 33 46 35 55 72 95 61 34 29 43 60 91 45 38 30 21
Peroxisomes 4 6 0 4 0 0 1 – 2 2 0 8 2 10 11 1 0 2 0 1 1 0 0 0
SpindlePole 66 81 82 118 222 60 180 – 136 193 176 236 105 164 108 53 51 50 87 71 60 87 134 112
Vac/Vac Membrane 2 5 1 1 2 1 1 – 2 1 2 1 1 1 0 7 7 11 4 10 1 3 4 5
Unique Cell Count 189 184 103 152 290 116 261 210 293 280 347 181 277 217 160 115 136 222 238 138 172 226 195
Labelled Cell Count 289 267 132 223 363 160 340 295 439 417 500 276 410 313 243 179 212 222 238 138 172 226 195


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.0 4.9 5.0 5.6 3.7 4.7 – 4.1 4.0 3.8 4.0 5.2 5.4 5.4 7.1 6.8 7.0 4.9 5.1 5.1
Std Deviation (1e-4) 1.0 0.9 0.9 1.5 1.4 1.3 1.8 – 1.3 1.2 1.0 1.1 0.9 1.6 1.7 2.1 1.8 1.8 1.7 1.6 1.6
Intensity Change (Log2) – – – 0.03 0.19 -0.41 -0.06 – -0.27 -0.29 -0.36 -0.29 0.1 0.14 0.15 0.54 0.48 0.53 -0.0 0.06 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0.7 1.8 0 0 – 0 0 0 0 -1.2 0 -1.0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.0 2.2 2.9 3.2 – 0 2.2 1.4 1.3 1.9 3.5 4.8 7.2 5.8 5.6
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 4.6 4.7 4.7 3.7 4.1 3.3
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 2.6 2.2 2.4
Nucleus 0.8 -1.8 -1.4 -0.6 – -2.1 -1.7 -1.5 -3.4 -0.2 -1.7 -1.7 -0.2 -0.1 0.2
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.5 -2.3 0 0 – 0 0 0 0 4.2 3.6 2.5 1.2 1.8 2.9
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole -0.4 -0.6 0 0 – 0 0 0 0 -3.7 -3.7 -5.1 -7.4 -5.3 -6.6
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.8113 -0.5327 -0.3854 -0.9445 -1.0488 -0.6889 1.4785 1.1263 0.8426 1.2412 0.4554 0.9538 1.8625 2.1981 1.8041 1.9589 1.6934 1.7755
Actin 0.0368 0.0003 0.0124 0.004 0.0049 0.0009 0.0253 0.0027 0.0142 0.0212 0.0018 0.0009 0.0079 0.0001 0.0018 0.0001 0.0007 0.0002
Bud 0.0003 0.0003 0.0002 0.0005 0.0004 0.0001 0.0022 0.0006 0.0005 0.0008 0.0005 0.0002 0.0003 0.0001 0.0001 0.0002 0.0001 0.0001
Bud Neck 0.0039 0.0076 0.0077 0.0045 0.0083 0.0041 0.0063 0.0076 0.0096 0.0212 0.0855 0.0145 0.0094 0.004 0.0064 0.0215 0.0054 0.0086
Bud Periphery 0.0003 0.0006 0.0003 0.0007 0.0006 0.0001 0.0054 0.0008 0.0007 0.001 0.001 0.0005 0.0006 0.0002 0.0002 0.0004 0.0006 0.0003
Bud Site 0.0023 0.0051 0.0025 0.0017 0.0154 0.0005 0.0037 0.0151 0.0037 0.0113 0.0051 0.0021 0.0057 0.0015 0.0078 0.0024 0.0006 0.0008
Cell Periphery 0.0003 0.0008 0.0005 0.0003 0.0003 0.0001 0.0006 0.0006 0.0006 0.001 0.0003 0.0003 0.0007 0.0001 0.0005 0.0008 0.0002 0.0003
Cytoplasm 0.0028 0.0039 0.0028 0.0332 0.0008 0.0014 0.01 0.0029 0.0084 0.0127 0.0099 0.0014 0.0039 0.0002 0.0008 0.0012 0.0003 0.001
Cytoplasmic Foci 0.0945 0.0579 0.05 0.0371 0.0537 0.0366 0.0496 0.0372 0.0375 0.0342 0.0189 0.0221 0.0535 0.0153 0.0268 0.0424 0.0278 0.0233
Eisosomes 0.0002 0.0002 0.0003 0.0001 0.0002 0 0.0003 0.0001 0.0001 0.0001 0.0001 0 0.0004 0 0.0001 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0009 0.0007 0.0011 0.0013 0.0003 0.0001 0.0016 0.0021 0.0009 0.006 0.0023 0.0006 0.0008 0.0001 0.0002 0.0002 0.0001 0.0054
Endosome 0.0223 0.0197 0.0245 0.0361 0.0096 0.0052 0.0549 0.0196 0.0184 0.0328 0.0172 0.0184 0.0162 0.0017 0.0106 0.0077 0.0055 0.0041
Golgi 0.0106 0.0052 0.02 0.0135 0.0073 0.0037 0.0333 0.0091 0.0109 0.0298 0.0091 0.0052 0.007 0.0014 0.0033 0.0025 0.0051 0.0025
Lipid Particles 0.2063 0.1714 0.1615 0.0843 0.1665 0.1145 0.1139 0.081 0.0888 0.1021 0.0598 0.0686 0.1564 0.0383 0.089 0.0847 0.1661 0.0717
Mitochondria 0.0016 0.0013 0.0194 0.0582 0.0034 0.0002 0.0389 0.0054 0.0031 0.0073 0.027 0.0007 0.0055 0.0002 0.0004 0.0012 0.0094 0.0003
None 0.0042 0.0133 0.0258 0.0232 0.0034 0.0009 0.038 0.0132 0.0024 0.0008 0.0046 0.0005 0.0164 0.0021 0.0015 0.0027 0.0009 0.0148
Nuclear Periphery 0.003 0.0035 0.013 0.0031 0.0022 0.004 0.0052 0.0022 0.0078 0.0036 0.0129 0.0063 0.0053 0.0019 0.0017 0.0004 0.0042 0.0025
Nucleolus 0.2123 0.3674 0.3702 0.4699 0.0894 0.2048 0.2101 0.4809 0.5185 0.489 0.2583 0.3255 0.2358 0.4321 0.4809 0.4515 0.2416 0.2111
Nucleus 0.0188 0.0353 0.0256 0.0358 0.0075 0.028 0.0505 0.0752 0.073 0.0399 0.0738 0.0283 0.0365 0.034 0.0443 0.0295 0.027 0.0209
Peroxisomes 0.0289 0.0074 0.0149 0.0056 0.0434 0.0099 0.0191 0.0071 0.0044 0.0048 0.0042 0.0065 0.0201 0.0027 0.0082 0.017 0.0472 0.0058
Punctate Nuclear 0.3468 0.2868 0.2431 0.1778 0.5802 0.5838 0.3134 0.2247 0.1858 0.1677 0.4017 0.4937 0.4136 0.4632 0.3104 0.3286 0.455 0.6245
Vacuole 0.0025 0.0103 0.0033 0.0054 0.0018 0.0008 0.0135 0.0109 0.0096 0.0117 0.0042 0.0031 0.0035 0.0007 0.0045 0.0046 0.0013 0.0016
Vacuole Periphery 0.0005 0.0009 0.001 0.0037 0.0005 0.0002 0.0041 0.001 0.0013 0.0012 0.0017 0.0005 0.0004 0.0001 0.0002 0.0003 0.0006 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.6214 4.3073 2.1308 9.3071 10.412 3.2105 9.4361 6.3687 10.4847 9.3928
Translational Efficiency 1.7034 2.3437 3.8698 1.0842 0.8862 3.1214 1.4302 1.5247 0.773 1.0627

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of UAF (upstream activation factor) complex; UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; targeting factor for the UAF that facilitates activation of many rDNA genes; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication
Localization
Cell Percentages nucleolus (53%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-1

Uaf30

Uaf30


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Uaf30-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available