Standard name
Human Ortholog
Description Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.09 0.12 0.16 0.15 – 0.22 0.34 0.25 0.08 0.07 0.05 0.18 0.09 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0 – 0.06 0.05 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0.1 0 0 0.05 0 0 0 0 0 0
Mitochondria 0 0.06 0.07 0.09 0.05 0.13 0.09 0.1 – 0.23 0.26 0.2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.08 0.14 0.06 0 0 0.06 0 0 – 0 0 0 0.05 0 0.09 0.13 0.14 0.1 0 0.08 0 0.06 0.05 0
Nuclear Periphery 0.12 0 0.18 0.31 0.1 0.12 0.2 0.14 – 0.11 0.07 0.1 0.06 0 0 0.05 0.07 0.05 0.08 0 0.1 0.07 0.06 0.06
Nucleolus 0 0 0.07 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.82 0.81 0.8 0.81 0.84 0.71 0.62 0.68 – 0.55 0.48 0.49 0.84 0.85 0.85 0.75 0.83 0.78 0.81 0.85 0.81 0.8 0.84 0.82
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 2 0 1 1 1 – 4 2 1 1 1 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 0 0 0 0 – 1 3 5 0 0 2 0 0 0 0 1 3 1 2 5
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 2 3 17 6 – 21 13 21 1 3 0 1 1 1 0 0 0 0 0 0
Cytoplasm 3 6 18 17 37 51 94 94 – 136 156 140 22 28 20 20 23 21 0 0 0 0 0 2
Endoplasmic Reticulum 0 0 5 2 6 8 34 22 – 38 25 25 6 8 4 6 9 6 0 0 0 1 1 1
Endosome 1 1 9 4 14 1 6 6 – 0 1 8 0 1 1 2 2 3 0 2 10 5 11 9
Golgi 1 0 5 3 11 1 9 2 – 2 1 2 14 14 38 0 6 9 0 0 0 0 0 3
Mitochondria 5 9 29 31 21 55 53 66 – 139 119 112 1 1 1 4 6 0 3 2 7 5 6 7
Nucleus 9 20 24 5 13 23 19 18 – 15 5 13 14 18 33 14 35 17 5 12 14 25 30 25
Nuclear Periphery 14 4 78 102 40 50 122 90 – 70 31 58 17 15 5 5 18 8 10 2 45 28 35 34
Nucleolus 2 6 31 17 13 5 6 11 – 6 3 7 4 4 8 1 5 2 4 1 2 7 9 11
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 1 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 94 119 341 267 352 293 375 441 – 337 219 273 221 345 313 83 203 137 108 143 362 307 541 490
Unique Cell Count 114 147 428 331 419 410 604 646 609 460 555 264 405 368 111 244 176 134 170 450 385 642 597
Labelled Cell Count 130 165 541 451 509 492 736 758 769 578 666 301 438 425 136 308 204 134 170 450 385 642 597


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.4 16.5 13.8 12.7 11.4 9.4 9.1 9.0 – 7.8 7.8 7.8 21.5 27.0 32.5 11.2 12.4 15.5 18.0 17.1 19.2
Std Deviation (1e-4) 5.1 5.4 4.2 3.6 3.1 3.2 2.6 2.3 – 2.3 2.2 2.0 7.1 8.6 9.2 4.3 4.4 6.1 6.9 6.0 8.0
Intensity Change (Log2) – – – -0.13 -0.28 -0.55 -0.61 -0.62 – -0.83 -0.82 -0.83 0.64 0.97 1.23 -0.31 -0.16 0.17 0.38 0.31 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 3.1 0 – 3.5 3.1 3.7 0 0 0 0 0 0
Cytoplasm 0.6 2.7 4.3 5.8 5.4 – 8.1 11.1 8.9 2.3 1.7 0.8 5.1 2.7 3.5
Endoplasmic Reticulum 0 0.3 0.9 3.7 2.3 – 4.0 3.5 3.0 0 0.9 0 0 2.2 0
Endosome -0.9 1.1 0 -1.5 -1.6 – 0 0 -0.8 0 0 0 0 0 0
Golgi 0 1.6 0 0.4 0 – 0 0 0 3.2 2.2 5.7 0 0 0
Mitochondria 1.3 -1.1 3.2 1.2 1.9 – 6.9 7.6 5.9 -4.0 -5.1 -4.8 -1.2 -2.4 -3.5
Nucleus -2.9 -1.8 0 -1.9 -2.3 – -2.6 -3.8 -2.7 -0.2 -0.8 1.8 2.6 3.8 1.8
Nuclear Periphery 4.0 -3.6 -2.4 0.8 -1.9 – -3.1 -5.2 -3.5 -4.4 -6.7 -7.8 -3.6 -3.9 -4.4
Nucleolus -1.2 -2.7 -4.3 -5.3 -4.6 – -5.3 -5.1 -4.8 -3.3 -4.5 -3.3 -2.5 -2.9 -3.0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0.3 1.6 -2.8 -6.0 -4.1 – -8.1 -9.9 -9.8 1.3 2.1 2.0 -1.1 1.1 -0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.3268 19.6288 18.4953 17.7709 16.511 18.4111 12.8461 15.5847 14.4253 14.4139 12.9856 14.1088 16.9409 18.8994 17.0673 15.0761 14.7576 16.8049
Actin 0 0 0 0 0 0 0.0055 0 0 0 0.0228 0.0014 0 0 0 0 0 0
Bud 0 0 0 0 0.0001 0 0.0001 0.0001 0.0002 0.0002 0.0001 0 0.0001 0 0.0001 0 0 0
Bud Neck 0 0 0 0 0 0 0.0002 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0 0 0 0
Bud Periphery 0.0001 0.0001 0.0001 0 0.0002 0 0.0005 0.0003 0.001 0.0006 0.0003 0.0001 0.0003 0 0.0004 0.0001 0 0.0001
Bud Site 0 0.0003 0 0 0 0 0.0003 0 0.0001 0.0003 0.0001 0 0.0001 0 0.0001 0 0 0
Cell Periphery 0.0011 0.0013 0.0012 0.0002 0.0006 0.0007 0.0019 0.0019 0.0022 0.0027 0.0004 0.0009 0.0018 0.0009 0.0014 0.0008 0.0006 0.0007
Cytoplasm 0.0001 0.0011 0.0001 0.0001 0.0001 0 0.0004 0.0001 0.0001 0 0.0001 0 0.0001 0 0 0 0 0
Cytoplasmic Foci 0.0001 0.0003 0 0 0.0001 0 0.0017 0 0.0003 0.0001 0.0009 0.0006 0.0001 0 0.0001 0 0 0
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0071 0.0018 0.0001 0 0.0001 0 0.0004 0.0003 0.0002 0.0001 0.0004 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Endosome 0.0046 0.0019 0.0035 0.0024 0.0024 0.0017 0.0108 0.0032 0.0121 0.0038 0.0083 0.0048 0.0034 0.0011 0.0043 0.0017 0.0035 0.0025
Golgi 0.0002 0 0.0001 0 0 0 0.0009 0.0001 0.0012 0.0003 0.0025 0.001 0.0002 0 0.0002 0 0 0.0001
Lipid Particles 0.0008 0.0004 0.001 0.0001 0.0001 0.0002 0.0028 0.0007 0.0021 0.0024 0.0021 0.0087 0.0028 0.0004 0.0012 0.0003 0.0004 0.0009
Mitochondria 0.0024 0.0003 0.0055 0.0001 0.0001 0.0002 0.0182 0.0047 0.0235 0.0431 0.0009 0.0046 0.0088 0.0003 0.0153 0.0005 0.0002 0.0021
None 0 0.0002 0 0 0 0 0.004 0 0 0 0.0002 0.0001 0 0 0 0 0 0
Nuclear Periphery 0.0015 0.0051 0.0022 0.0006 0.0011 0.0067 0.0131 0.0053 0.0057 0.0004 0.0042 0.0136 0.0088 0.0057 0.0018 0.0016 0.0055 0.0095
Nucleolus 0.0013 0.001 0.0043 0.0001 0.0006 0.0006 0.0078 0.0006 0.0034 0.0022 0.0003 0.0015 0.0035 0.0014 0.0018 0.0005 0.0002 0.0062
Nucleus 0.0009 0.0068 0.0015 0.0002 0.0008 0.0007 0.004 0.0007 0.0058 0.0009 0.0004 0.0006 0.0049 0.0005 0.0011 0.0006 0.0004 0.001
Peroxisomes 0 0 0 0 0 0 0.0017 0 0.0002 0.0002 0.001 0.0008 0.0001 0 0.0001 0 0 0
Punctate Nuclear 0 0.0004 0 0 0 0 0.0003 0 0 0 0.0004 0.0004 0 0 0 0 0 0
Vacuole 0.3029 0.2921 0.3272 0.2821 0.3414 0.2383 0.2761 0.2799 0.2714 0.2649 0.2459 0.1642 0.2428 0.192 0.2394 0.2612 0.2459 0.162
Vacuole Periphery 0.6767 0.6869 0.6531 0.714 0.6524 0.7506 0.6494 0.7022 0.6703 0.6778 0.7082 0.7964 0.7221 0.7973 0.7323 0.7324 0.7431 0.8148

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.8235 35.8682 27.1703 42.2434 41.1843 39.2661 47.7884 62.2132 58.0287 75.2835
Translational Efficiency 1.2027 0.8228 1.0717 0.7214 0.6362 1.0435 0.9897 0.6244 0.8863 0.7736

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
37 1339 121 1310 1693 1564 256 1720 1730 2903 377 3030

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1495.78 1511.69 2042.31 1485.16 1959.28 1325.57 1669.68 1438.52 1949.37 1411.42 1789.28 1458.68
Standard Deviation 290.95 652.34 366.23 319.88 612.84 248.92 300.20 327.87 611.43 488.13 366.75 325.26
Intensity Change Log 2 — 0.015264 0.449304 -0.010280 — -0.563711 -0.230752 -0.445738 — -0.284212 0.103482 -0.240925

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000459 0.000123 0.000152 0.000133 0.000038 0.000046 0.000274 0.000099 0.000047 0.000081 0.000235 0.000113
Bud Neck 0.007897 0.007619 0.001651 0.001096 0.000096 0.000590 0.008310 0.000607 0.000263 0.003832 0.006173 0.000818
Bud Site 0.004844 0.011940 0.000559 0.003279 0.000380 0.001088 0.006121 0.002743 0.000475 0.006094 0.004336 0.002975
Cell Periphery 0.000150 0.004931 0.000086 0.000747 0.002376 0.000445 0.000235 0.001424 0.002328 0.002514 0.000187 0.001132
Cytoplasm 0.002559 0.020456 0.000171 0.000890 0.000481 0.000403 0.000791 0.000629 0.000526 0.009653 0.000592 0.000742
Cytoplasmic Foci 0.019511 0.002481 0.008566 0.001324 0.000188 0.001353 0.007912 0.001702 0.000602 0.001873 0.008122 0.001538
Eisosomes 0.000029 0.000398 0.000041 0.000017 0.000015 0.000023 0.000090 0.000026 0.000015 0.000196 0.000074 0.000022
Endoplasmic Reticulum 0.000796 0.002503 0.000136 0.000346 0.000257 0.000596 0.000708 0.000466 0.000269 0.001476 0.000524 0.000414
Endosome 0.050772 0.004016 0.001527 0.003981 0.000252 0.001789 0.020187 0.006419 0.001332 0.002816 0.014198 0.005365
Golgi 0.060225 0.002872 0.002444 0.010672 0.001104 0.000572 0.006042 0.025920 0.002368 0.001633 0.004887 0.019328
Lipid Particles 0.000776 0.001969 0.000511 0.000835 0.000567 0.000605 0.002832 0.001509 0.000571 0.001234 0.002087 0.001217
Mitochondria 0.009714 0.003719 0.035963 0.014518 0.001020 0.007694 0.015270 0.022506 0.001206 0.005860 0.021911 0.019053
Mitotic Spindle 0.010687 0.000141 0.000494 0.000460 0.000016 0.000218 0.002075 0.000370 0.000244 0.000182 0.001567 0.000409
None 0.000253 0.013328 0.000760 0.000353 0.000290 0.000562 0.000537 0.000587 0.000289 0.006451 0.000609 0.000486
Nuclear Periphery 0.002193 0.000537 0.015159 0.002087 0.000148 0.002915 0.009285 0.000873 0.000192 0.001818 0.011170 0.001398
Nuclear Periphery Foci 0.002803 0.001033 0.006878 0.001129 0.000162 0.002002 0.006922 0.000811 0.000219 0.001555 0.006908 0.000948
Nucleolus 0.018081 0.007483 0.023641 0.001592 0.000989 0.004919 0.037260 0.000805 0.001354 0.006102 0.032889 0.001145
Nucleus 0.017083 0.016002 0.001663 0.000618 0.000533 0.002855 0.014875 0.000279 0.000886 0.008919 0.010635 0.000426
Peroxisomes 0.001081 0.000348 0.001124 0.000072 0.000030 0.000280 0.004027 0.000111 0.000053 0.000311 0.003095 0.000094
Vacuole 0.403512* 0.195653 0.227725* 0.362981* 0.155735 0.309282* 0.443813* 0.347803* 0.161035 0.256871* 0.374459* 0.354365*
Vacuole Periphery 0.386576* 0.702449* 0.670747* 0.592869* 0.835323* 0.661763* 0.412433* 0.584311* 0.825726* 0.680529* 0.495340* 0.588011*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.14 1.03 1.06 -0.13 0.20 -0.59 -3.95 -2.46 -2.27 2.80 -2.26 -4.29 -1.63 -0.81 2.12
Bud Neck 0.03 1.37 1.49 4.75 1.35 -4.35 -7.09 -7.01 -0.10 6.64 -5.56 -7.23 -4.30 4.71 6.56
Bud Site -2.67 2.55 0.86 4.03 -2.58 -2.00 -2.66 -5.05 -2.98 1.54 -6.27 -2.61 -4.66 3.02 0.88
Cell Periphery -6.55 2.35 -4.57 5.65 -5.13 8.40 9.76 3.01 -4.02 -5.08 -0.46 10.03 4.68 3.74 -6.53
Cytoplasm -4.04 1.25 0.99 7.07 -1.00 0.06 -0.69 -0.67 -0.48 0.37 -7.18 -0.14 -0.53 6.88 -0.31
Cytoplasmic Foci 1.76 0.92 1.89 2.22 1.14 -2.25 -4.69 -7.28 -0.47 3.74 -3.27 -3.21 -3.29 1.02 2.81
Eisosomes -3.39 -0.94 1.17 3.51 2.93 -1.60 -6.03 -2.64 -0.96 5.23 -3.59 -6.38 -1.88 3.45 5.96
Endoplasmic Reticulum -3.40 2.70 1.91 4.82 -2.63 -4.41 -3.35 -3.68 1.54 1.76 -5.69 -2.62 -3.32 5.01 1.15
Endosome 2.04 2.14 2.04 0.05 -3.82 -6.61 -5.01 -12.54 -8.57 3.44 -2.59 -4.62 -6.73 -6.07 3.21
Golgi 2.31 2.33 1.99 -5.35 -5.26 1.92 -4.16 -11.31 -11.64 -8.05 1.09 -2.49 -11.26 -12.67 -8.98
Lipid Particles -2.87 0.81 -0.22 3.68 -2.48 -0.61 -2.19 -6.49 -6.02 1.27 -4.55 -2.15 -6.94 0.11 1.23
Mitochondria 1.20 -2.15 -0.94 -5.06 1.87 -6.20 -6.49 -10.86 -6.64 -2.46 -6.84 -5.26 -12.33 -8.46 0.68
Mitotic Spindle 1.16 1.11 1.12 -2.50 0.17 -1.91 -3.27 -3.09 -0.96 2.67 0.28 -2.80 -0.81 -2.18 2.63
None -5.61 -3.73 -0.91 5.57 3.58 -0.96 -4.22 -4.96 -0.08 -0.75 -5.64 -5.61 -3.78 5.46 2.14
Nuclear Periphery 2.46 -2.09 0.10 -4.08 2.11 -4.86 -4.18 -11.95 3.57 3.85 -5.25 -4.43 -7.28 1.20 3.94
Nuclear Periphery Foci 1.63 -1.61 1.55 -0.36 2.45 -3.13 -3.66 -9.72 2.02 3.31 -4.08 -4.58 -8.38 1.83 4.08
Nucleolus 1.15 -0.52 1.84 4.66 3.23 -5.33 -6.69 1.17 5.67 6.72 -6.45 -7.34 0.78 7.05 7.40
Nucleus 0.07 1.31 1.40 7.03 2.32 -4.23 -4.77 1.05 5.20 4.86 -7.28 -4.66 1.31 8.08 4.94
Peroxisomes 1.97 -0.11 2.75 5.37 2.38 -2.70 -4.77 -3.39 1.81 4.67 -4.56 -5.16 -2.13 3.88 5.09
Vacuole 5.41 4.18 1.16 -20.35 -6.82 -19.79 -20.17 -28.09 -4.76 6.61 -15.15 -16.41 -31.89 -16.62 1.55
Vacuole Periphery -7.48 -5.90 -4.95 10.19 3.00 21.06* 26.84 33.41* 8.75 -10.70 19.87 21.71 35.59 13.44 -6.18
Standard name
Human Ortholog
Description Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication
Localization
Cell Percentages vacuolar membrane (87%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Cot1

Cot1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cot1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available