Standard name | |
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Human Ortholog | |
Description | Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 1 | – | 4 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 3 | 5 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 2 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 1 | 2 | 3 | 17 | 6 | – | 21 | 13 | 21 | 1 | 3 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 6 | 18 | 17 | 37 | 51 | 94 | 94 | – | 136 | 156 | 140 | 22 | 28 | 20 | 20 | 23 | 21 | 0 | 0 | 0 | 0 | 0 | 2 |
Endoplasmic Reticulum | 0 | 0 | 5 | 2 | 6 | 8 | 34 | 22 | – | 38 | 25 | 25 | 6 | 8 | 4 | 6 | 9 | 6 | 0 | 0 | 0 | 1 | 1 | 1 |
Endosome | 1 | 1 | 9 | 4 | 14 | 1 | 6 | 6 | – | 0 | 1 | 8 | 0 | 1 | 1 | 2 | 2 | 3 | 0 | 2 | 10 | 5 | 11 | 9 |
Golgi | 1 | 0 | 5 | 3 | 11 | 1 | 9 | 2 | – | 2 | 1 | 2 | 14 | 14 | 38 | 0 | 6 | 9 | 0 | 0 | 0 | 0 | 0 | 3 |
Mitochondria | 5 | 9 | 29 | 31 | 21 | 55 | 53 | 66 | – | 139 | 119 | 112 | 1 | 1 | 1 | 4 | 6 | 0 | 3 | 2 | 7 | 5 | 6 | 7 |
Nucleus | 9 | 20 | 24 | 5 | 13 | 23 | 19 | 18 | – | 15 | 5 | 13 | 14 | 18 | 33 | 14 | 35 | 17 | 5 | 12 | 14 | 25 | 30 | 25 |
Nuclear Periphery | 14 | 4 | 78 | 102 | 40 | 50 | 122 | 90 | – | 70 | 31 | 58 | 17 | 15 | 5 | 5 | 18 | 8 | 10 | 2 | 45 | 28 | 35 | 34 |
Nucleolus | 2 | 6 | 31 | 17 | 13 | 5 | 6 | 11 | – | 6 | 3 | 7 | 4 | 4 | 8 | 1 | 5 | 2 | 4 | 1 | 2 | 7 | 9 | 11 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 94 | 119 | 341 | 267 | 352 | 293 | 375 | 441 | – | 337 | 219 | 273 | 221 | 345 | 313 | 83 | 203 | 137 | 108 | 143 | 362 | 307 | 541 | 490 |
Unique Cell Count | 114 | 147 | 428 | 331 | 419 | 410 | 604 | 646 | 609 | 460 | 555 | 264 | 405 | 368 | 111 | 244 | 176 | 134 | 170 | 450 | 385 | 642 | 597 | |
Labelled Cell Count | 130 | 165 | 541 | 451 | 509 | 492 | 736 | 758 | 769 | 578 | 666 | 301 | 438 | 425 | 136 | 308 | 204 | 134 | 170 | 450 | 385 | 642 | 597 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.4 | 16.5 | 13.8 | 12.7 | 11.4 | 9.4 | 9.1 | 9.0 | – | 7.8 | 7.8 | 7.8 | 21.5 | 27.0 | 32.5 | 11.2 | 12.4 | 15.5 | 18.0 | 17.1 | 19.2 |
Std Deviation (1e-4) | 5.1 | 5.4 | 4.2 | 3.6 | 3.1 | 3.2 | 2.6 | 2.3 | – | 2.3 | 2.2 | 2.0 | 7.1 | 8.6 | 9.2 | 4.3 | 4.4 | 6.1 | 6.9 | 6.0 | 8.0 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.28 | -0.55 | -0.61 | -0.62 | – | -0.83 | -0.82 | -0.83 | 0.64 | 0.97 | 1.23 | -0.31 | -0.16 | 0.17 | 0.38 | 0.31 | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 3.1 | 0 | – | 3.5 | 3.1 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | 2.7 | 4.3 | 5.8 | 5.4 | – | 8.1 | 11.1 | 8.9 | 2.3 | 1.7 | 0.8 | 5.1 | 2.7 | 3.5 |
Endoplasmic Reticulum | 0 | 0.3 | 0.9 | 3.7 | 2.3 | – | 4.0 | 3.5 | 3.0 | 0 | 0.9 | 0 | 0 | 2.2 | 0 |
Endosome | -0.9 | 1.1 | 0 | -1.5 | -1.6 | – | 0 | 0 | -0.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 1.6 | 0 | 0.4 | 0 | – | 0 | 0 | 0 | 3.2 | 2.2 | 5.7 | 0 | 0 | 0 |
Mitochondria | 1.3 | -1.1 | 3.2 | 1.2 | 1.9 | – | 6.9 | 7.6 | 5.9 | -4.0 | -5.1 | -4.8 | -1.2 | -2.4 | -3.5 |
Nucleus | -2.9 | -1.8 | 0 | -1.9 | -2.3 | – | -2.6 | -3.8 | -2.7 | -0.2 | -0.8 | 1.8 | 2.6 | 3.8 | 1.8 |
Nuclear Periphery | 4.0 | -3.6 | -2.4 | 0.8 | -1.9 | – | -3.1 | -5.2 | -3.5 | -4.4 | -6.7 | -7.8 | -3.6 | -3.9 | -4.4 |
Nucleolus | -1.2 | -2.7 | -4.3 | -5.3 | -4.6 | – | -5.3 | -5.1 | -4.8 | -3.3 | -4.5 | -3.3 | -2.5 | -2.9 | -3.0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.3 | 1.6 | -2.8 | -6.0 | -4.1 | – | -8.1 | -9.9 | -9.8 | 1.3 | 2.1 | 2.0 | -1.1 | 1.1 | -0.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 17.3268 | 19.6288 | 18.4953 | 17.7709 | 16.511 | 18.4111 | 12.8461 | 15.5847 | 14.4253 | 14.4139 | 12.9856 | 14.1088 | 16.9409 | 18.8994 | 17.0673 | 15.0761 | 14.7576 | 16.8049 |
Actin | 0 | 0 | 0 | 0 | 0 | 0 | 0.0055 | 0 | 0 | 0 | 0.0228 | 0.0014 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Bud Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0005 | 0.0003 | 0.001 | 0.0006 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0004 | 0.0001 | 0 | 0.0001 |
Bud Site | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Cell Periphery | 0.0011 | 0.0013 | 0.0012 | 0.0002 | 0.0006 | 0.0007 | 0.0019 | 0.0019 | 0.0022 | 0.0027 | 0.0004 | 0.0009 | 0.0018 | 0.0009 | 0.0014 | 0.0008 | 0.0006 | 0.0007 |
Cytoplasm | 0.0001 | 0.0011 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0.0017 | 0 | 0.0003 | 0.0001 | 0.0009 | 0.0006 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0071 | 0.0018 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Endosome | 0.0046 | 0.0019 | 0.0035 | 0.0024 | 0.0024 | 0.0017 | 0.0108 | 0.0032 | 0.0121 | 0.0038 | 0.0083 | 0.0048 | 0.0034 | 0.0011 | 0.0043 | 0.0017 | 0.0035 | 0.0025 |
Golgi | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0009 | 0.0001 | 0.0012 | 0.0003 | 0.0025 | 0.001 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0.0001 |
Lipid Particles | 0.0008 | 0.0004 | 0.001 | 0.0001 | 0.0001 | 0.0002 | 0.0028 | 0.0007 | 0.0021 | 0.0024 | 0.0021 | 0.0087 | 0.0028 | 0.0004 | 0.0012 | 0.0003 | 0.0004 | 0.0009 |
Mitochondria | 0.0024 | 0.0003 | 0.0055 | 0.0001 | 0.0001 | 0.0002 | 0.0182 | 0.0047 | 0.0235 | 0.0431 | 0.0009 | 0.0046 | 0.0088 | 0.0003 | 0.0153 | 0.0005 | 0.0002 | 0.0021 |
None | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.004 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0015 | 0.0051 | 0.0022 | 0.0006 | 0.0011 | 0.0067 | 0.0131 | 0.0053 | 0.0057 | 0.0004 | 0.0042 | 0.0136 | 0.0088 | 0.0057 | 0.0018 | 0.0016 | 0.0055 | 0.0095 |
Nucleolus | 0.0013 | 0.001 | 0.0043 | 0.0001 | 0.0006 | 0.0006 | 0.0078 | 0.0006 | 0.0034 | 0.0022 | 0.0003 | 0.0015 | 0.0035 | 0.0014 | 0.0018 | 0.0005 | 0.0002 | 0.0062 |
Nucleus | 0.0009 | 0.0068 | 0.0015 | 0.0002 | 0.0008 | 0.0007 | 0.004 | 0.0007 | 0.0058 | 0.0009 | 0.0004 | 0.0006 | 0.0049 | 0.0005 | 0.0011 | 0.0006 | 0.0004 | 0.001 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0017 | 0 | 0.0002 | 0.0002 | 0.001 | 0.0008 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Punctate Nuclear | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0.0004 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.3029 | 0.2921 | 0.3272 | 0.2821 | 0.3414 | 0.2383 | 0.2761 | 0.2799 | 0.2714 | 0.2649 | 0.2459 | 0.1642 | 0.2428 | 0.192 | 0.2394 | 0.2612 | 0.2459 | 0.162 |
Vacuole Periphery | 0.6767 | 0.6869 | 0.6531 | 0.714 | 0.6524 | 0.7506 | 0.6494 | 0.7022 | 0.6703 | 0.6778 | 0.7082 | 0.7964 | 0.7221 | 0.7973 | 0.7323 | 0.7324 | 0.7431 | 0.8148 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.8235 | 35.8682 | 27.1703 | 42.2434 | 41.1843 | 39.2661 | 47.7884 | 62.2132 | 58.0287 | 75.2835 |
Translational Efficiency | 1.2027 | 0.8228 | 1.0717 | 0.7214 | 0.6362 | 1.0435 | 0.9897 | 0.6244 | 0.8863 | 0.7736 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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