Standard name | |
---|---|
Human Ortholog | |
Description | Ty1 enhancer activator involved in Ty enhancer-mediated transcription; required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 2 | 1 | 3 | 3 | 3 | 7 | 6 | 8 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 5 |
Bud Neck | 0 | 0 | 1 | 0 | 3 | 0 | 5 | 7 | 5 | 4 | 0 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 |
Bud Site | 0 | 1 | 0 | 2 | 6 | 12 | 10 | 3 | 16 | 11 | 17 | 8 | 1 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 2 | 7 | 13 | 14 | 19 | 27 | 30 | 23 | 26 | 29 | 17 | 20 | 27 | 43 | 14 | 18 | 13 | 0 | 0 | 0 | 2 | 1 | 1 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 4 | 0 | 0 | 0 | 1 | 2 | 1 |
Endosome | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 4 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 4 | 0 | 5 | 2 | 0 | 3 | 1 | 3 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 3 | 3 | 0 | 1 | 2 | 2 | 0 |
Mitochondria | 16 | 27 | 11 | 25 | 19 | 137 | 198 | 101 | 260 | 331 | 326 | 179 | 32 | 9 | 17 | 4 | 5 | 7 | 2 | 0 | 4 | 1 | 4 | 6 |
Nucleus | 356 | 211 | 363 | 267 | 336 | 384 | 379 | 408 | 274 | 294 | 276 | 257 | 349 | 344 | 337 | 161 | 142 | 97 | 350 | 222 | 360 | 224 | 250 | 214 |
Nuclear Periphery | 0 | 0 | 1 | 1 | 3 | 1 | 0 | 0 | 2 | 0 | 2 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Nucleolus | 31 | 16 | 6 | 7 | 6 | 7 | 5 | 8 | 17 | 5 | 13 | 9 | 11 | 20 | 26 | 2 | 3 | 2 | 11 | 3 | 9 | 19 | 37 | 32 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 9 | 3 | 13 | 12 | 22 | 36 | 18 | 15 | 23 | 25 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 3 | 3 |
Vac/Vac Membrane | 3 | 0 | 2 | 3 | 2 | 2 | 0 | 7 | 7 | 3 | 3 | 6 | 7 | 3 | 12 | 12 | 13 | 10 | 9 | 5 | 14 | 16 | 40 | 54 |
Unique Cell Count | 373 | 219 | 381 | 291 | 365 | 460 | 485 | 517 | 413 | 474 | 458 | 375 | 381 | 382 | 404 | 194 | 177 | 127 | 384 | 237 | 399 | 275 | 357 | 338 |
Labelled Cell Count | 412 | 257 | 402 | 323 | 404 | 578 | 651 | 607 | 630 | 696 | 698 | 517 | 423 | 406 | 436 | 201 | 185 | 141 | 384 | 237 | 399 | 275 | 357 | 338 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.6 | 6.0 | 6.2 | 5.5 | 5.7 | 4.4 | 4.2 | 4.5 | 3.7 | 3.6 | 3.4 | 4.0 | 6.3 | 6.2 | 6.0 | 9.2 | 9.1 | 9.3 | 6.2 | 6.4 | 6.8 |
Std Deviation (1e-4) | 1.5 | 1.1 | 0.9 | 1.1 | 1.0 | 1.1 | 0.9 | 0.9 | 1.1 | 0.9 | 0.8 | 0.8 | 1.5 | 1.7 | 1.8 | 1.9 | 1.9 | 2.0 | 1.6 | 1.8 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.15 | -0.1 | -0.49 | -0.55 | -0.45 | -0.74 | -0.76 | -0.86 | -0.63 | 0.02 | 0.02 | -0.03 | 0.57 | 0.56 | 0.59 | 0.01 | 0.05 | 0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.0 | 1.6 | 1.9 | 2.8 | 3.0 | 2.8 | 2.8 | 3.2 | 2.1 | 2.5 | 3.5 | 5.0 | 3.3 | 4.4 | 4.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.3 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | -0.5 | 1.0 | -0.6 | 0 | 0 |
Nucleus | -1.9 | -1.8 | -5.4 | -7.1 | -7.0 | -10.2 | -11.5 | -11.9 | -9.6 | -2.0 | -2.8 | -5.3 | -4.9 | -5.6 | -6.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.8 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.2 | 2.8 | 3.4 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -1.3 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.3751 | 1.6736 | 1.5519 | 1.4938 | 1.1807 | 1.5273 | 2.8281 | 2.8347 | 2.2739 | 2.1646 | 2.3798 | 2.4214 | 1.6936 | 1.8334 | 1.6655 | 1.605 | 1.2994 | 1.5106 |
Actin | 0.0337 | 0 | 0.0172 | 0.0375 | 0.0222 | 0.0137 | 0.0363 | 0 | 0.007 | 0.0316 | 0.0057 | 0.0056 | 0.0221 | 0 | 0.0169 | 0.0042 | 0.0236 | 0.0006 |
Bud | 0.0008 | 0 | 0.0006 | 0.0006 | 0.0003 | 0.0002 | 0.0012 | 0 | 0.0004 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0 | 0.0002 | 0.0003 | 0.0003 | 0.0001 |
Bud Neck | 0.0022 | 0 | 0.0025 | 0.0032 | 0.0006 | 0.0008 | 0.0042 | 0.0001 | 0.0017 | 0.0009 | 0.0005 | 0.0011 | 0.0014 | 0 | 0.0001 | 0.0042 | 0.001 | 0.0005 |
Bud Periphery | 0.0014 | 0 | 0.0012 | 0.0014 | 0.0004 | 0.0005 | 0.0035 | 0 | 0.0007 | 0.0005 | 0.0006 | 0.0004 | 0.0005 | 0 | 0.0006 | 0.0005 | 0.0008 | 0.0001 |
Bud Site | 0.0047 | 0 | 0.0154 | 0.0033 | 0.001 | 0.0003 | 0.0046 | 0 | 0.0023 | 0.0035 | 0.0012 | 0.0034 | 0.0023 | 0 | 0.0005 | 0.0019 | 0.0007 | 0.0001 |
Cell Periphery | 0.0005 | 0 | 0.0012 | 0.001 | 0 | 0.0001 | 0.0015 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0003 | 0 |
Cytoplasm | 0.005 | 0 | 0.0079 | 0.0017 | 0.0004 | 0.0002 | 0.0069 | 0 | 0.0011 | 0.0003 | 0.0018 | 0.0005 | 0.0065 | 0 | 0.0001 | 0.0016 | 0.0024 | 0 |
Cytoplasmic Foci | 0.0274 | 0 | 0.0069 | 0.0113 | 0.0103 | 0.0018 | 0.0161 | 0 | 0.0046 | 0.0142 | 0.0152 | 0.007 | 0.0093 | 0 | 0.0001 | 0.0034 | 0.0077 | 0.0012 |
Eisosomes | 0.0003 | 0 | 0.0003 | 0.0006 | 0 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0002 | 0.0002 | 0.0006 | 0 |
Endoplasmic Reticulum | 0.0071 | 0 | 0.0031 | 0.002 | 0.0002 | 0.0005 | 0.0083 | 0 | 0.0006 | 0.0003 | 0.0024 | 0.0004 | 0.0028 | 0 | 0.0003 | 0.0013 | 0.004 | 0 |
Endosome | 0.0415 | 0 | 0.0095 | 0.0138 | 0.005 | 0.0034 | 0.0403 | 0 | 0.0052 | 0.0079 | 0.0358 | 0.0053 | 0.0084 | 0 | 0.0004 | 0.017 | 0.0183 | 0.0019 |
Golgi | 0.0098 | 0 | 0.0047 | 0.0078 | 0.0079 | 0.0066 | 0.0136 | 0 | 0.0052 | 0.0146 | 0.0168 | 0.0044 | 0.0028 | 0 | 0.0004 | 0.0025 | 0.0073 | 0.0033 |
Lipid Particles | 0.0176 | 0 | 0.0095 | 0.0101 | 0.0179 | 0.004 | 0.0237 | 0 | 0.0137 | 0.021 | 0.0171 | 0.009 | 0.0096 | 0 | 0.0001 | 0.0054 | 0.0165 | 0.0008 |
Mitochondria | 0.0124 | 0.0001 | 0.0072 | 0.0666 | 0.0015 | 0.0076 | 0.0227 | 0.0001 | 0.0164 | 0.0103 | 0.0122 | 0.0055 | 0.0031 | 0.0001 | 0.0012 | 0.001 | 0.0089 | 0.0019 |
None | 0.0082 | 0 | 0.0055 | 0.0016 | 0.0001 | 0.0002 | 0.0044 | 0 | 0.0002 | 0.0003 | 0.001 | 0.0002 | 0.014 | 0 | 0.0002 | 0.0051 | 0.0081 | 0 |
Nuclear Periphery | 0.0287 | 0.0004 | 0.006 | 0.0035 | 0.0014 | 0.0132 | 0.0645 | 0.0003 | 0.0074 | 0.0013 | 0.0271 | 0.0009 | 0.0072 | 0.0004 | 0.0006 | 0.0023 | 0.0385 | 0.0002 |
Nucleolus | 0.0145 | 0.023 | 0.0208 | 0.008 | 0.0528 | 0.0391 | 0.0231 | 0.0179 | 0.0193 | 0.0114 | 0.0619 | 0.0371 | 0.0118 | 0.0119 | 0.0098 | 0.0071 | 0.0381 | 0.0467 |
Nucleus | 0.7484 | 0.9761 | 0.8514 | 0.8009 | 0.8533 | 0.9031 | 0.6828 | 0.9813 | 0.9036 | 0.8215 | 0.7858 | 0.9091 | 0.886 | 0.9874 | 0.9676 | 0.934 | 0.7439 | 0.9404 |
Peroxisomes | 0.0105 | 0 | 0.0175 | 0.0086 | 0.0231 | 0.0012 | 0.0156 | 0 | 0.0055 | 0.0512 | 0.0013 | 0.0045 | 0.0031 | 0 | 0.0002 | 0.0011 | 0.0155 | 0.0013 |
Punctate Nuclear | 0.0147 | 0.0003 | 0.0034 | 0.013 | 0.0013 | 0.0016 | 0.0109 | 0.0002 | 0.0033 | 0.0076 | 0.0057 | 0.0038 | 0.0058 | 0.0001 | 0.0003 | 0.003 | 0.0576 | 0.0006 |
Vacuole | 0.0054 | 0 | 0.0067 | 0.0026 | 0.0002 | 0.0006 | 0.0102 | 0 | 0.001 | 0.0003 | 0.0046 | 0.0009 | 0.0013 | 0 | 0.0001 | 0.0028 | 0.0024 | 0.0001 |
Vacuole Periphery | 0.005 | 0 | 0.0015 | 0.001 | 0.0001 | 0.0011 | 0.0053 | 0 | 0.0005 | 0.0003 | 0.0026 | 0.0004 | 0.0007 | 0 | 0.0002 | 0.0007 | 0.0035 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.7605 | 13.8439 | 13.804 | 15.6305 | 21.9612 | 10.1573 | 14.8724 | 14.7615 | 14.1779 | 17.29 |
Translational Efficiency | 0.5514 | 0.3982 | 0.5837 | 0.3927 | 0.3718 | 0.6955 | 0.4087 | 0.3722 | 0.3952 | 0.4318 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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