Standard name | |
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Human Ortholog | |
Description | Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 3 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 8 | 6 | 3 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 11 | 20 | 10 | 15 | 33 | 61 | 68 | 64 | 80 | 79 | 96 | 8 | 22 | 33 | 26 | 14 | 20 | 0 | 3 | 2 | 15 | 14 | 14 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 1 | 1 | 0 | 2 | 1 | 0 | 1 | 4 | 1 | 2 | 0 | 1 | 0 | 1 | 3 | 2 |
Endosome | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 3 | 1 | 2 | 3 |
Golgi | 0 | 4 | 37 | 4 | 8 | 23 | 52 | 63 | 11 | 17 | 20 | 25 | 3 | 12 | 9 | 8 | 0 | 9 | 0 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 3 | 3 | 2 | 0 | 0 | 0 | 0 | 4 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | 1 |
Nucleus | 18 | 144 | 200 | 76 | 70 | 48 | 97 | 72 | 52 | 55 | 51 | 53 | 14 | 31 | 21 | 23 | 10 | 9 | 55 | 276 | 313 | 99 | 78 | 64 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 3 | 1 |
Nucleolus | 12 | 11 | 9 | 3 | 5 | 4 | 7 | 8 | 11 | 5 | 3 | 8 | 4 | 9 | 8 | 0 | 1 | 3 | 5 | 14 | 48 | 7 | 8 | 14 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 |
Vac/Vac Membrane | 6 | 112 | 99 | 39 | 158 | 99 | 180 | 155 | 113 | 130 | 96 | 143 | 32 | 67 | 51 | 22 | 14 | 40 | 6 | 40 | 54 | 15 | 22 | 43 |
Unique Cell Count | 34 | 265 | 331 | 128 | 238 | 189 | 337 | 327 | 242 | 269 | 222 | 295 | 59 | 131 | 115 | 72 | 35 | 69 | 70 | 350 | 445 | 155 | 143 | 158 |
Labelled Cell Count | 39 | 283 | 366 | 133 | 257 | 210 | 401 | 375 | 259 | 293 | 254 | 331 | 63 | 141 | 125 | 88 | 42 | 87 | 70 | 350 | 445 | 155 | 143 | 158 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 38.6 | 32.8 | 32.7 | 32.1 | 28.6 | 31.8 | 30.3 | 28.5 | 24.7 | 26.7 | 25.4 | 24.7 | 39.0 | 34.3 | 34.2 | 33.3 | 34.2 | 36.0 | 34.1 | 33.0 | 38.2 |
Std Deviation (1e-4) | 11.0 | 8.4 | 8.0 | 6.9 | 7.4 | 7.6 | 6.6 | 6.9 | 6.4 | 7.3 | 7.1 | 7.4 | 12.7 | 11.5 | 12.5 | 11.9 | 15.8 | 17.3 | 13.0 | 14.8 | 15.7 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.19 | -0.04 | -0.11 | -0.2 | -0.41 | -0.29 | -0.36 | -0.4 | 0.26 | 0.07 | 0.06 | 0.03 | 0.07 | 0.14 | 0.06 | 0.01 | 0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.7 | 0.1 | 4.1 | 4.8 | 5.6 | 6.8 | 7.7 | 8.9 | 8.5 | 0 | 3.6 | 6.5 | 7.3 | 0 | 5.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -2.7 | -3.4 | 0.3 | 1.6 | 2.9 | -2.8 | -2.1 | -0.8 | -1.1 | -1.4 | -0.6 | -1.0 | 0 | 0 | 0.4 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -0.2 | -7.3 | -7.7 | -8.2 | -10.0 | -9.3 | -9.9 | -8.7 | -10.8 | -5.2 | -7.1 | -7.8 | -4.4 | -3.6 | -7.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | -0.5 | 0 | -0.5 | -0.2 | 1.2 | -0.7 | 0 | 0 | 0 | 2.1 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.1 | 8.6 | 5.1 | 6.2 | 4.6 | 4.1 | 4.6 | 3.2 | 4.8 | 3.6 | 4.3 | 2.8 | 0.1 | 1.2 | 4.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 34.4128 | 40.5512 | 36.7601 | 36.6684 | 35.6708 | 36.6529 | 37.1501 | 46.1961 | 38.7445 | 38.6972 | 38.1478 | 40.3618 | 30.1117 | 36.3718 | 31.628 | 31.8768 | 24.7309 | 35.5109 |
Actin | 0.0469 | 0.0001 | 0.0154 | 0 | 0.0417 | 0.008 | 0.002 | 0.0002 | 0.0016 | 0.0001 | 0.0212 | 0.0002 | 0.0271 | 0 | 0.0142 | 0.0002 | 0.0209 | 0.0001 |
Bud | 0.0008 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0 | 0.0006 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0004 | 0 |
Bud Neck | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0015 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0004 |
Bud Periphery | 0.0011 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0005 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0011 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0006 | 0.0001 |
Bud Site | 0.001 | 0.0001 | 0.0022 | 0 | 0.0003 | 0.0003 | 0.0009 | 0.0001 | 0.0007 | 0 | 0.0004 | 0.0001 | 0.0028 | 0 | 0.0006 | 0.0002 | 0.0011 | 0 |
Cell Periphery | 0.0007 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0004 | 0 | 0.0001 | 0.0003 | 0.0004 | 0 |
Cytoplasm | 0.0092 | 0.0011 | 0.0027 | 0.0008 | 0.0007 | 0.005 | 0.0204 | 0.0012 | 0.016 | 0.0007 | 0.0012 | 0.0018 | 0.0176 | 0.0005 | 0.0008 | 0.0018 | 0.0057 | 0.0003 |
Cytoplasmic Foci | 0.0125 | 0.0008 | 0.0054 | 0 | 0.005 | 0.0014 | 0.0079 | 0 | 0.0054 | 0 | 0.0025 | 0.0002 | 0.0054 | 0 | 0.0165 | 0.006 | 0.0181 | 0 |
Eisosomes | 0.0005 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.0037 | 0 | 0.0005 | 0 | 0.0001 | 0.0006 | 0.0024 | 0 | 0.0016 | 0 | 0.0011 | 0.0005 | 0.0013 | 0 | 0.0001 | 0.0001 | 0.0019 | 0 |
Endosome | 0.0142 | 0.0001 | 0.0056 | 0 | 0.0018 | 0.003 | 0.006 | 0 | 0.0145 | 0 | 0.0043 | 0.0035 | 0.0142 | 0 | 0.0017 | 0.0005 | 0.027 | 0 |
Golgi | 0.0088 | 0 | 0.0041 | 0 | 0.0049 | 0.0044 | 0.0005 | 0 | 0.0035 | 0 | 0.0016 | 0.0001 | 0.0045 | 0 | 0.0018 | 0.0001 | 0.0187 | 0 |
Lipid Particles | 0.0057 | 0.0002 | 0.0034 | 0 | 0.014 | 0.0031 | 0.0032 | 0 | 0.0073 | 0 | 0.0054 | 0.0002 | 0.0023 | 0 | 0.0026 | 0.0067 | 0.021 | 0 |
Mitochondria | 0.008 | 0.0015 | 0.0022 | 0.0004 | 0.0028 | 0.012 | 0.0047 | 0.0012 | 0.0028 | 0.0008 | 0.0104 | 0.0021 | 0.006 | 0.0004 | 0.002 | 0.0029 | 0.0606 | 0.0003 |
None | 0.0057 | 0.0002 | 0.0038 | 0 | 0.0003 | 0.0007 | 0.0133 | 0 | 0.0014 | 0 | 0.001 | 0.0002 | 0.015 | 0 | 0.0008 | 0.0011 | 0.0116 | 0 |
Nuclear Periphery | 0.0262 | 0.0101 | 0.018 | 0.0191 | 0.028 | 0.0123 | 0.0333 | 0.0312 | 0.0261 | 0.0214 | 0.0538 | 0.0244 | 0.0118 | 0.0118 | 0.0077 | 0.0173 | 0.0195 | 0.0193 |
Nucleolus | 0.0098 | 0.0123 | 0.0088 | 0.002 | 0.011 | 0.0297 | 0.0192 | 0.0064 | 0.0084 | 0.0021 | 0.0417 | 0.0161 | 0.0303 | 0.0057 | 0.024 | 0.0296 | 0.1031 | 0.0118 |
Nucleus | 0.8199 | 0.9711 | 0.9158 | 0.9775 | 0.8683 | 0.91 | 0.8329 | 0.9583 | 0.8924 | 0.9734 | 0.8363 | 0.9455 | 0.8441 | 0.981 | 0.9204 | 0.9255 | 0.659 | 0.9665 |
Peroxisomes | 0.0057 | 0.0001 | 0.0035 | 0 | 0.0194 | 0.0008 | 0.0003 | 0 | 0.0019 | 0 | 0.0013 | 0.0001 | 0.002 | 0 | 0.0026 | 0.0003 | 0.0097 | 0 |
Punctate Nuclear | 0.0106 | 0.0003 | 0.006 | 0.0001 | 0.0005 | 0.001 | 0.0427 | 0.0002 | 0.0101 | 0.0001 | 0.0058 | 0.0014 | 0.0064 | 0.0001 | 0.0024 | 0.0017 | 0.0147 | 0.0001 |
Vacuole | 0.0031 | 0.0007 | 0.0005 | 0.0001 | 0.0003 | 0.0024 | 0.006 | 0.0002 | 0.0035 | 0.0004 | 0.0018 | 0.0013 | 0.004 | 0.0001 | 0.0005 | 0.0022 | 0.0019 | 0.0003 |
Vacuole Periphery | 0.0054 | 0.0011 | 0.0011 | 0.0002 | 0.0004 | 0.0028 | 0.0029 | 0.0006 | 0.002 | 0.0007 | 0.0081 | 0.0017 | 0.0032 | 0.0002 | 0.0007 | 0.0029 | 0.0033 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 171.174 | 113.7689 | 136.7627 | 158.9872 | 123.7554 | 104.3011 | 171.3971 | 168.0875 | 180.1412 | 136.6761 |
Translational Efficiency | 1.3549 | 1.7675 | 1.6123 | 1.3515 | 1.3537 | 2.312 | 1.411 | 1.5069 | 1.3322 | 1.3819 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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