Standard name | |
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Human Ortholog | |
Description | Component of RQC, which mediates nascent chain degradation; RQC (ribosome quality control complex) is a ribosome-bound complex required for degradation of polypeptides arising from stalled translation; recruits alanine- and threonine-charged tRNA to the A site and directs the elongation of nascent chains independently of mRNA or 40S subunits; monitors translation stress and signals this to Hsf1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 5 | 1 | 5 | 1 | 6 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 0 | 1 | 3 | 1 | 3 | 9 | 7 | 5 | 11 | 5 | 11 | 0 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 3 | 3 | 8 | 14 | 14 | 24 | 19 | 20 | 24 | 15 | 22 | 12 | 9 | 21 | 28 | 15 | 28 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 174 | 126 | 146 | 274 | 463 | 306 | 515 | 435 | 252 | 403 | 178 | 278 | 322 | 560 | 587 | 373 | 205 | 441 | 172 | 121 | 151 | 229 | 407 | 403 |
Endoplasmic Reticulum | 2 | 2 | 2 | 2 | 10 | 11 | 14 | 30 | 20 | 32 | 15 | 25 | 2 | 9 | 15 | 20 | 8 | 20 | 1 | 1 | 4 | 7 | 16 | 31 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 0 | 2 | 4 | 6 | 21 | 50 | 133 | 162 | 144 | 236 | 136 | 210 | 0 | 0 | 7 | 3 | 3 | 7 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 1 | 2 | 0 | 0 | 2 | 0 | 2 | 5 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 0 | 1 | 0 | 0 | 17 | 59 | 2 | 7 | 4 | 9 | 0 | 1 | 0 | 23 | 1 | 21 | 0 | 3 | 5 | 2 | 6 | 4 |
Unique Cell Count | 178 | 130 | 148 | 280 | 479 | 337 | 623 | 620 | 366 | 603 | 285 | 446 | 329 | 571 | 607 | 407 | 216 | 490 | 176 | 130 | 163 | 243 | 441 | 451 |
Labelled Cell Count | 183 | 134 | 156 | 294 | 509 | 386 | 720 | 723 | 446 | 723 | 355 | 562 | 339 | 582 | 639 | 453 | 234 | 524 | 176 | 130 | 163 | 243 | 441 | 451 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.2 | 12.2 | 8.4 | 8.5 | 8.2 | 7.0 | 6.5 | 6.3 | 5.5 | 5.3 | 5.1 | 4.9 | 14.1 | 13.2 | 11.5 | 16.0 | 16.2 | 17.7 | 13.6 | 12.6 | 14.5 |
Std Deviation (1e-4) | 1.9 | 1.9 | 1.7 | 1.1 | 1.2 | 0.9 | 1.0 | 1.0 | 0.9 | 0.8 | 0.8 | 0.8 | 1.9 | 1.8 | 1.6 | 3.1 | 3.3 | 4.0 | 2.0 | 2.1 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.02 | -0.04 | -0.27 | -0.37 | -0.43 | -0.63 | -0.66 | -0.71 | -0.77 | 0.74 | 0.65 | 0.45 | 0.93 | 0.95 | 1.07 | 0.69 | 0.59 | 0.78 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 1.2 | 1.1 | 0 | 1.7 | 1.1 | 1.6 | 1.5 | 0 | 0 | 0 | 2.2 | 2.1 | 1.8 |
Cytoplasm | -0.6 | -1.3 | -3.2 | -5.0 | -7.3 | -7.3 | -7.8 | -8.2 | -8.5 | -0.6 | -0.5 | -1.3 | -3.0 | -1.9 | -3.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1.9 | 2.1 | 2.1 | 2.0 | 2.2 | 0 | 0 | 0 | 1.9 | 0 | 1.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0.9 | 3.9 | 5.3 | 6.2 | 8.3 | 8.5 | 9.5 | 9.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.4574 | 11.7366 | 10.1166 | 9.307 | 9.883 | 10.3133 | 10.4483 | 15.1396 | 13.7452 | 13.3305 | 10.2525 | 13.6922 | 5.7251 | 8.4584 | 7.6081 | 7.4787 | 7.4164 | 7.5148 |
Actin | 0.0905 | 0.001 | 0.0233 | 0.002 | 0.003 | 0.0053 | 0.0222 | 0.0067 | 0.0079 | 0.0111 | 0.0081 | 0.0011 | 0.0104 | 0.0028 | 0.0039 | 0.0012 | 0.0004 | 0.0019 |
Bud | 0.0026 | 0.0006 | 0.0009 | 0.0027 | 0.0006 | 0.0004 | 0.0007 | 0.0027 | 0.0005 | 0.001 | 0.0071 | 0.0002 | 0.0005 | 0.0004 | 0.0006 | 0.0006 | 0.0002 | 0.0003 |
Bud Neck | 0.0084 | 0.0006 | 0.0015 | 0.0019 | 0.0012 | 0.0056 | 0.0036 | 0.0006 | 0.0009 | 0.0007 | 0.0011 | 0.001 | 0.0008 | 0.0003 | 0.0004 | 0.0006 | 0.0004 | 0.0012 |
Bud Periphery | 0.0021 | 0.0005 | 0.0008 | 0.0031 | 0.0005 | 0.0007 | 0.0009 | 0.0024 | 0.0002 | 0.001 | 0.0164 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0003 |
Bud Site | 0.0099 | 0.0015 | 0.0015 | 0.0011 | 0.0002 | 0.0003 | 0.0033 | 0.0096 | 0.0043 | 0.0015 | 0.0017 | 0.0001 | 0.0018 | 0.0009 | 0.0023 | 0.0005 | 0.0002 | 0.0002 |
Cell Periphery | 0.0015 | 0.0015 | 0.0014 | 0.0006 | 0.0006 | 0.0011 | 0.0007 | 0.0006 | 0.0005 | 0.0003 | 0.0005 | 0 | 0.0009 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0002 |
Cytoplasm | 0.6103 | 0.9229 | 0.8042 | 0.8441 | 0.8076 | 0.7961 | 0.754 | 0.9371 | 0.8697 | 0.8209 | 0.722 | 0.9169 | 0.7285 | 0.9124 | 0.8783 | 0.7938 | 0.8803 | 0.8872 |
Cytoplasmic Foci | 0.0238 | 0.0038 | 0.012 | 0.0188 | 0.0091 | 0.0065 | 0.0165 | 0.0056 | 0.018 | 0.0134 | 0.0122 | 0.0087 | 0.0434 | 0.0146 | 0.0212 | 0.0264 | 0.0201 | 0.0098 |
Eisosomes | 0.0012 | 0 | 0.0011 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0704 | 0.0378 | 0.0533 | 0.0134 | 0.0719 | 0.0981 | 0.0577 | 0.008 | 0.0149 | 0.0069 | 0.009 | 0.0291 | 0.1221 | 0.0118 | 0.0275 | 0.0167 | 0.0299 | 0.0448 |
Endosome | 0.0584 | 0.0131 | 0.0391 | 0.0491 | 0.049 | 0.0285 | 0.049 | 0.0078 | 0.0372 | 0.048 | 0.0737 | 0.0254 | 0.0446 | 0.0255 | 0.0339 | 0.1222 | 0.0565 | 0.0259 |
Golgi | 0.0152 | 0.002 | 0.008 | 0.0043 | 0.0021 | 0.009 | 0.0194 | 0.0035 | 0.0114 | 0.0176 | 0.0237 | 0.0053 | 0.0112 | 0.0131 | 0.0053 | 0.01 | 0.0024 | 0.0046 |
Lipid Particles | 0.0092 | 0.0001 | 0.0052 | 0.0002 | 0.0004 | 0.0051 | 0.0049 | 0.0004 | 0.004 | 0.009 | 0.0277 | 0.0003 | 0.0041 | 0.0062 | 0.0033 | 0.0003 | 0.0001 | 0.0008 |
Mitochondria | 0.0097 | 0.0011 | 0.0081 | 0.0177 | 0.0021 | 0.0208 | 0.0136 | 0.0038 | 0.0042 | 0.0127 | 0.0636 | 0.0021 | 0.0017 | 0.003 | 0.0032 | 0.0018 | 0.0004 | 0.0137 |
None | 0.0119 | 0.0004 | 0.0024 | 0.0011 | 0.0014 | 0.0007 | 0.0018 | 0.0007 | 0.0016 | 0.0006 | 0.0007 | 0.0005 | 0.0064 | 0.0006 | 0.0044 | 0.0009 | 0.0007 | 0.0014 |
Nuclear Periphery | 0.0123 | 0.0017 | 0.0075 | 0.0039 | 0.0186 | 0.0043 | 0.0164 | 0.0008 | 0.0031 | 0.0024 | 0.0027 | 0.0015 | 0.0081 | 0.0011 | 0.0019 | 0.0016 | 0.001 | 0.0012 |
Nucleolus | 0.0029 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0006 | 0.0002 | 0.0005 | 0.0003 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 |
Nucleus | 0.0086 | 0.0012 | 0.0047 | 0.0035 | 0.0031 | 0.0017 | 0.0157 | 0.0015 | 0.003 | 0.0019 | 0.0018 | 0.0014 | 0.0023 | 0.0009 | 0.001 | 0.0009 | 0.0007 | 0.0008 |
Peroxisomes | 0.0167 | 0 | 0.0017 | 0.0006 | 0.0001 | 0.0008 | 0.002 | 0.0005 | 0.0031 | 0.0019 | 0.0025 | 0.0004 | 0.0016 | 0.0027 | 0.0032 | 0.0002 | 0.0001 | 0.0009 |
Punctate Nuclear | 0.0079 | 0 | 0.0016 | 0.0004 | 0.0007 | 0.0006 | 0.0011 | 0.0001 | 0.0006 | 0.0003 | 0.0004 | 0.0001 | 0.0036 | 0.0003 | 0.0007 | 0.0002 | 0.0001 | 0.0001 |
Vacuole | 0.0214 | 0.0088 | 0.018 | 0.0248 | 0.0189 | 0.0103 | 0.0134 | 0.0059 | 0.0128 | 0.0401 | 0.0198 | 0.0043 | 0.0056 | 0.0025 | 0.0069 | 0.0163 | 0.0053 | 0.0033 |
Vacuole Periphery | 0.005 | 0.0013 | 0.0033 | 0.0064 | 0.0087 | 0.0039 | 0.0028 | 0.0011 | 0.002 | 0.0082 | 0.005 | 0.0015 | 0.0015 | 0.0006 | 0.0012 | 0.0054 | 0.0011 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 65.3122 | 58.8598 | 70.6808 | 43.0141 | 60.8136 | 82.4353 | 48.7943 | 50.641 | 59.8574 | 47.728 |
Translational Efficiency | 1.0654 | 0.878 | 0.623 | 1.1912 | 0.8392 | 0.6786 | 0.8271 | 0.732 | 0.626 | 1.0292 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1581 | 523 | 319 | 1222 | 1573 | 1115 | 485 | 1108 | 3154 | 1638 | 804 | 2330 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1028.02 | 1142.92 | 1791.41 | 1616.70 | 1116.26 | 1258.82 | 1576.75 | 1575.36 | 1072.03 | 1221.81 | 1661.92 | 1597.04 |
Standard Deviation | 183.38 | 226.24 | 221.72 | 253.25 | 197.76 | 197.19 | 315.45 | 271.48 | 195.73 | 213.85 | 300.93 | 262.89 |
Intensity Change Log 2 | — | 0.152856 | 0.801227 | 0.653184 | — | 0.173399 | 0.498281 | 0.497008 | — | 0.163587 | 0.651467 | 0.573994 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000132 | 0.000351 | 0.001169 | 0.000809 | 0.000152 | 0.000350 | 0.000992 | 0.000637 | 0.000142 | 0.000350 | 0.001062 | 0.000727 |
Bud Neck | 0.000321 | 0.000746 | 0.005462 | 0.001808 | 0.000185 | 0.000459 | 0.003023 | 0.001376 | 0.000253 | 0.000550 | 0.003991 | 0.001603 |
Bud Site | 0.013626 | 0.029845 | 0.024920 | 0.021643 | 0.009959 | 0.020894 | 0.038952 | 0.018864 | 0.011797 | 0.023752 | 0.033385 | 0.020321 |
Cell Periphery | 0.000182 | 0.000455 | 0.003590 | 0.000968 | 0.000269 | 0.000256 | 0.001213 | 0.005243 | 0.000226 | 0.000320 | 0.002156 | 0.003001 |
Cytoplasm | 0.833223* | 0.822107* | 0.725891* | 0.746191* | 0.888774* | 0.833272* | 0.544673* | 0.876914* | 0.860928* | 0.829707* | 0.616574* | 0.808355* |
Cytoplasmic Foci | 0.092970 | 0.073092 | 0.046366 | 0.030297 | 0.059861 | 0.095499 | 0.050940 | 0.011886 | 0.076457 | 0.088345 | 0.049125 | 0.021542 |
Eisosomes | 0.000004 | 0.000006 | 0.000008 | 0.000007 | 0.000002 | 0.000006 | 0.000009 | 0.000006 | 0.000003 | 0.000006 | 0.000009 | 0.000007 |
Endoplasmic Reticulum | 0.005886 | 0.003483 | 0.003591 | 0.001225 | 0.003551 | 0.002386 | 0.005980 | 0.000649 | 0.004722 | 0.002736 | 0.005032 | 0.000951 |
Endosome | 0.004408 | 0.008046 | 0.011957 | 0.010522 | 0.002344 | 0.004927 | 0.017349 | 0.001982 | 0.003379 | 0.005923 | 0.015209 | 0.006461 |
Golgi | 0.009786 | 0.010596 | 0.017856 | 0.013381 | 0.009902 | 0.014439 | 0.014309 | 0.006468 | 0.009844 | 0.013212 | 0.015716 | 0.010093 |
Lipid Particles | 0.000871 | 0.002193 | 0.018408 | 0.007932 | 0.000606 | 0.000786 | 0.016209 | 0.011283 | 0.000738 | 0.001235 | 0.017082 | 0.009525 |
Mitochondria | 0.000478 | 0.000114 | 0.000033 | 0.001701 | 0.000385 | 0.000601 | 0.000533 | 0.001314 | 0.000431 | 0.000445 | 0.000335 | 0.001517 |
Mitotic Spindle | 0.000530 | 0.000763 | 0.006147 | 0.004813 | 0.000446 | 0.004082 | 0.002957 | 0.001679 | 0.000488 | 0.003022 | 0.004223 | 0.003323 |
None | 0.001777 | 0.002362 | 0.000743 | 0.000221 | 0.001196 | 0.000612 | 0.000666 | 0.000282 | 0.001487 | 0.001170 | 0.000697 | 0.000250 |
Nuclear Periphery | 0.000275 | 0.000132 | 0.000220 | 0.000094 | 0.000034 | 0.000020 | 0.000346 | 0.000030 | 0.000155 | 0.000056 | 0.000296 | 0.000064 |
Nuclear Periphery Foci | 0.000878 | 0.001469 | 0.015410 | 0.001567 | 0.000992 | 0.000339 | 0.007087 | 0.002171 | 0.000935 | 0.000700 | 0.010389 | 0.001854 |
Nucleolus | 0.000108 | 0.000615 | 0.000047 | 0.000208 | 0.000052 | 0.000078 | 0.000156 | 0.000090 | 0.000080 | 0.000249 | 0.000113 | 0.000152 |
Nucleus | 0.004906 | 0.006243 | 0.010644 | 0.003740 | 0.002704 | 0.001907 | 0.005228 | 0.003467 | 0.003808 | 0.003292 | 0.007377 | 0.003610 |
Peroxisomes | 0.000198 | 0.000802 | 0.000266 | 0.000856 | 0.000120 | 0.000459 | 0.000546 | 0.000403 | 0.000159 | 0.000568 | 0.000435 | 0.000640 |
Vacuole | 0.015991 | 0.029239 | 0.102523 | 0.142713 | 0.008542 | 0.012804 | 0.273550* | 0.052446 | 0.012276 | 0.018051 | 0.205692* | 0.099788 |
Vacuole Periphery | 0.013449 | 0.007345 | 0.004750 | 0.009303 | 0.009925 | 0.005825 | 0.015280 | 0.002811 | 0.011691 | 0.006310 | 0.011102 | 0.006216 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.79 | -7.12 | -3.51 | -2.24 | 1.91 | -3.64 | -4.11 | -5.62 | -3.05 | 1.76 | -5.34 | -6.68 | -5.49 | -3.33 | 2.33 |
Bud Neck | -4.54 | -9.58 | -13.84 | -8.37 | 6.51 | -6.85 | -7.51 | -12.35 | -9.07 | 4.70 | -7.18 | -11.75 | -18.51 | -13.43 | 7.51 |
Bud Site | -6.69 | -4.43 | -4.31 | 3.05 | 1.36 | -7.70 | -6.74 | -7.12 | 1.06 | 4.47 | -9.52 | -7.74 | -7.58 | 2.28 | 4.49 |
Cell Periphery | -4.15 | -8.94 | -9.25 | -4.42 | 6.92 | 0.28 | -4.79 | -7.31 | -7.33 | -5.72 | -2.63 | -9.85 | -8.34 | -8.02 | -2.10 |
Cytoplasm | 1.06 | 7.46 | 10.31 | 6.86 | -1.32 | 7.21 | 23.43 | 2.47 | -4.77 | -21.93 | 4.93 | 22.03 | 9.96 | 3.90 | -16.05 |
Cytoplasmic Foci | 3.41 | 9.32 | 16.47 | 8.76 | 5.14 | -7.33 | 2.27 | 16.93 | 19.97 | 10.32 | -3.07 | 8.16 | 22.79 | 20.28 | 10.44 |
Eisosomes | -1.52 | -4.33 | -2.67 | -0.59 | 1.35 | -4.61 | -6.71 | -6.00 | -0.03 | 3.41 | -3.80 | -7.59 | -5.05 | -0.68 | 3.28 |
Endoplasmic Reticulum | 2.12 | 2.19 | 5.94 | 2.64 | 2.84 | 1.16 | -1.90 | 4.60 | 1.96 | 5.50 | 2.53 | 0.04 | 7.47 | 2.78 | 6.05 |
Endosome | -3.00 | -6.16 | -4.22 | -0.91 | 1.64 | -4.93 | -10.22 | 0.86 | 4.85 | 10.24 | -4.59 | -11.58 | -3.82 | -0.03 | 7.85 |
Golgi | -0.54 | -4.63 | -1.05 | -0.44 | 2.50 | -3.20 | -1.30 | 2.96 | 4.74 | 2.66 | -3.13 | -3.20 | 0.68 | 2.83 | 3.10 |
Lipid Particles | -1.91 | -10.37 | -9.59 | -5.99 | 5.90 | -2.01 | -8.20 | -11.24 | -11.04 | 2.66 | -2.05 | -12.21 | -14.78 | -13.34 | 5.53 |
Mitochondria | 4.16 | 5.30 | -1.87 | -2.60 | -2.76 | -1.15 | -0.27 | -0.96 | -0.70 | -0.83 | -0.11 | 0.74 | -1.82 | -1.75 | -1.98 |
Mitotic Spindle | -0.89 | -6.73 | -3.42 | -3.21 | 1.21 | -3.01 | -3.81 | -1.61 | 1.75 | 1.36 | -3.05 | -7.18 | -3.78 | -0.12 | 1.28 |
None | -0.35 | 2.45 | 4.30 | 1.26 | 1.89 | 1.81 | 2.39 | 5.93 | 1.14 | 1.69 | 0.50 | 3.39 | 6.27 | 1.60 | 2.55 |
Nuclear Periphery | 0.74 | 0.22 | 1.03 | 0.45 | 4.20 | 1.11 | -3.09 | 0.28 | -1.08 | 3.13 | 1.08 | -1.35 | 1.03 | -0.27 | 3.81 |
Nuclear Periphery Foci | -1.91 | -7.65 | -3.45 | -0.73 | 7.20 | 4.25 | -5.81 | -5.79 | -11.35 | 4.65 | 1.46 | -9.51 | -6.30 | -8.29 | 8.46 |
Nucleolus | -1.17 | 3.26 | -3.23 | 0.97 | -5.88 | -1.06 | -5.10 | -3.44 | -0.45 | 3.16 | -1.22 | -2.46 | -4.22 | 0.76 | -1.72 |
Nucleus | -0.66 | -4.16 | 0.41 | 0.80 | 4.51 | 3.28 | -3.94 | -3.17 | -5.89 | 2.59 | 0.62 | -5.30 | -1.20 | -1.25 | 4.93 |
Peroxisomes | -2.72 | -1.10 | -6.54 | 0.12 | -4.75 | -6.05 | -6.50 | -6.62 | 0.84 | 2.10 | -4.97 | -5.82 | -8.58 | -0.35 | -2.30 |
Vacuole | -3.69 | -9.05 | -24.05 | -19.07 | -4.02 | -3.55 | -21.51 | -16.35 | -14.61 | 17.36 | -4.00 | -21.90 | -27.44 | -24.67 | 10.65 |
Vacuole Periphery | 3.24 | 6.50 | 3.61 | -0.51 | -3.16 | 3.34 | -1.93 | 6.98 | 3.24 | 6.17 | 5.18 | 1.24 | 6.76 | 0.77 | 3.73 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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