Standard name
Human Ortholog
Description Component of RQC, which mediates nascent chain degradation; RQC (ribosome quality control complex) is a ribosome-bound complex required for degradation of polypeptides arising from stalled translation; recruits alanine- and threonine-charged tRNA to the A site and directs the elongation of nascent chains independently of mRNA or 40S subunits; monitors translation stress and signals this to Hsf1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0 0 0 0.07 0.07 0.06 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.99 0.98 0.97 0.91 0.83 0.7 0.69 0.67 0.62 0.62 0.98 0.98 0.97 0.92 0.95 0.9 0.98 0.94 0.93 0.94 0.92 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.05 0.05 0.05 0.05 0.06 0 0 0 0.05 0 0 0 0 0 0 0 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.15 0.21 0.26 0.39 0.39 0.48 0.47 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 5 5 1 5 1 6 0 1 3 0 0 0 0 0 0 0 1 1
Bud 0 0 1 3 1 3 9 7 5 11 5 11 0 1 3 0 0 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – – – – – –
Cell Periphery 6 3 3 8 14 14 24 19 20 24 15 22 12 9 21 28 15 28 0 0 0 0 0 0
Cytoplasm 174 126 146 274 463 306 515 435 252 403 178 278 322 560 587 373 205 441 172 121 151 229 407 403
Endoplasmic Reticulum 2 2 2 2 10 11 14 30 20 32 15 25 2 9 15 20 8 20 1 1 4 7 16 31
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 1
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 1 1 0 1 2 1 0 0 0 0 0 1
Mitochondria 0 2 4 6 21 50 133 162 144 236 136 210 0 0 7 3 3 7 0 0 0 0 0 1
Nucleus 0 0 0 0 0 0 3 4 1 2 0 0 2 0 2 5 0 2 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 1 1 3 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 1 0 0 17 59 2 7 4 9 0 1 0 23 1 21 0 3 5 2 6 4
Unique Cell Count 178 130 148 280 479 337 623 620 366 603 285 446 329 571 607 407 216 490 176 130 163 243 441 451
Labelled Cell Count 183 134 156 294 509 386 720 723 446 723 355 562 339 582 639 453 234 524 176 130 163 243 441 451


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.2 12.2 8.4 8.5 8.2 7.0 6.5 6.3 5.5 5.3 5.1 4.9 14.1 13.2 11.5 16.0 16.2 17.7 13.6 12.6 14.5
Std Deviation (1e-4) 1.9 1.9 1.7 1.1 1.2 0.9 1.0 1.0 0.9 0.8 0.8 0.8 1.9 1.8 1.6 3.1 3.3 4.0 2.0 2.1 2.2
Intensity Change (Log2) – – – 0.02 -0.04 -0.27 -0.37 -0.43 -0.63 -0.66 -0.71 -0.77 0.74 0.65 0.45 0.93 0.95 1.07 0.69 0.59 0.78

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70005101520WT3HU80HU120HU16005101520WT3rpd3Δ_1rpd3Δ_2rpd3Δ_305101520WT1AF100AF140AF18005101520
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.2 1.1 0 1.7 1.1 1.6 1.5 0 0 0 2.2 2.1 1.8
Cytoplasm -0.6 -1.3 -3.2 -5.0 -7.3 -7.3 -7.8 -8.2 -8.5 -0.6 -0.5 -1.3 -3.0 -1.9 -3.4
Endoplasmic Reticulum 0 0 0 0 1.9 2.1 2.1 2.0 2.2 0 0 0 1.9 0 1.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.9 3.9 5.3 6.2 8.3 8.5 9.5 9.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.9 0 0 0 0 0 0 0 3.0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.4574 11.7366 10.1166 9.307 9.883 10.3133 10.4483 15.1396 13.7452 13.3305 10.2525 13.6922 5.7251 8.4584 7.6081 7.4787 7.4164 7.5148
Actin 0.0905 0.001 0.0233 0.002 0.003 0.0053 0.0222 0.0067 0.0079 0.0111 0.0081 0.0011 0.0104 0.0028 0.0039 0.0012 0.0004 0.0019
Bud 0.0026 0.0006 0.0009 0.0027 0.0006 0.0004 0.0007 0.0027 0.0005 0.001 0.0071 0.0002 0.0005 0.0004 0.0006 0.0006 0.0002 0.0003
Bud Neck 0.0084 0.0006 0.0015 0.0019 0.0012 0.0056 0.0036 0.0006 0.0009 0.0007 0.0011 0.001 0.0008 0.0003 0.0004 0.0006 0.0004 0.0012
Bud Periphery 0.0021 0.0005 0.0008 0.0031 0.0005 0.0007 0.0009 0.0024 0.0002 0.001 0.0164 0.0001 0.0004 0.0002 0.0002 0.0002 0 0.0003
Bud Site 0.0099 0.0015 0.0015 0.0011 0.0002 0.0003 0.0033 0.0096 0.0043 0.0015 0.0017 0.0001 0.0018 0.0009 0.0023 0.0005 0.0002 0.0002
Cell Periphery 0.0015 0.0015 0.0014 0.0006 0.0006 0.0011 0.0007 0.0006 0.0005 0.0003 0.0005 0 0.0009 0.0002 0.0005 0.0001 0.0001 0.0002
Cytoplasm 0.6103 0.9229 0.8042 0.8441 0.8076 0.7961 0.754 0.9371 0.8697 0.8209 0.722 0.9169 0.7285 0.9124 0.8783 0.7938 0.8803 0.8872
Cytoplasmic Foci 0.0238 0.0038 0.012 0.0188 0.0091 0.0065 0.0165 0.0056 0.018 0.0134 0.0122 0.0087 0.0434 0.0146 0.0212 0.0264 0.0201 0.0098
Eisosomes 0.0012 0 0.0011 0 0 0.0001 0.0001 0.0001 0 0.0001 0.0001 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0704 0.0378 0.0533 0.0134 0.0719 0.0981 0.0577 0.008 0.0149 0.0069 0.009 0.0291 0.1221 0.0118 0.0275 0.0167 0.0299 0.0448
Endosome 0.0584 0.0131 0.0391 0.0491 0.049 0.0285 0.049 0.0078 0.0372 0.048 0.0737 0.0254 0.0446 0.0255 0.0339 0.1222 0.0565 0.0259
Golgi 0.0152 0.002 0.008 0.0043 0.0021 0.009 0.0194 0.0035 0.0114 0.0176 0.0237 0.0053 0.0112 0.0131 0.0053 0.01 0.0024 0.0046
Lipid Particles 0.0092 0.0001 0.0052 0.0002 0.0004 0.0051 0.0049 0.0004 0.004 0.009 0.0277 0.0003 0.0041 0.0062 0.0033 0.0003 0.0001 0.0008
Mitochondria 0.0097 0.0011 0.0081 0.0177 0.0021 0.0208 0.0136 0.0038 0.0042 0.0127 0.0636 0.0021 0.0017 0.003 0.0032 0.0018 0.0004 0.0137
None 0.0119 0.0004 0.0024 0.0011 0.0014 0.0007 0.0018 0.0007 0.0016 0.0006 0.0007 0.0005 0.0064 0.0006 0.0044 0.0009 0.0007 0.0014
Nuclear Periphery 0.0123 0.0017 0.0075 0.0039 0.0186 0.0043 0.0164 0.0008 0.0031 0.0024 0.0027 0.0015 0.0081 0.0011 0.0019 0.0016 0.001 0.0012
Nucleolus 0.0029 0 0.0003 0.0001 0.0001 0.0001 0.0003 0.0006 0.0002 0.0005 0.0003 0 0.0003 0 0.0001 0 0 0
Nucleus 0.0086 0.0012 0.0047 0.0035 0.0031 0.0017 0.0157 0.0015 0.003 0.0019 0.0018 0.0014 0.0023 0.0009 0.001 0.0009 0.0007 0.0008
Peroxisomes 0.0167 0 0.0017 0.0006 0.0001 0.0008 0.002 0.0005 0.0031 0.0019 0.0025 0.0004 0.0016 0.0027 0.0032 0.0002 0.0001 0.0009
Punctate Nuclear 0.0079 0 0.0016 0.0004 0.0007 0.0006 0.0011 0.0001 0.0006 0.0003 0.0004 0.0001 0.0036 0.0003 0.0007 0.0002 0.0001 0.0001
Vacuole 0.0214 0.0088 0.018 0.0248 0.0189 0.0103 0.0134 0.0059 0.0128 0.0401 0.0198 0.0043 0.0056 0.0025 0.0069 0.0163 0.0053 0.0033
Vacuole Periphery 0.005 0.0013 0.0033 0.0064 0.0087 0.0039 0.0028 0.0011 0.002 0.0082 0.005 0.0015 0.0015 0.0006 0.0012 0.0054 0.0011 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 65.3122 58.8598 70.6808 43.0141 60.8136 82.4353 48.7943 50.641 59.8574 47.728
Translational Efficiency 1.0654 0.878 0.623 1.1912 0.8392 0.6786 0.8271 0.732 0.626 1.0292

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1581 523 319 1222 1573 1115 485 1108 3154 1638 804 2330

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1028.02 1142.92 1791.41 1616.70 1116.26 1258.82 1576.75 1575.36 1072.03 1221.81 1661.92 1597.04
Standard Deviation 183.38 226.24 221.72 253.25 197.76 197.19 315.45 271.48 195.73 213.85 300.93 262.89
Intensity Change Log 2 — 0.152856 0.801227 0.653184 — 0.173399 0.498281 0.497008 — 0.163587 0.651467 0.573994

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000132 0.000351 0.001169 0.000809 0.000152 0.000350 0.000992 0.000637 0.000142 0.000350 0.001062 0.000727
Bud Neck 0.000321 0.000746 0.005462 0.001808 0.000185 0.000459 0.003023 0.001376 0.000253 0.000550 0.003991 0.001603
Bud Site 0.013626 0.029845 0.024920 0.021643 0.009959 0.020894 0.038952 0.018864 0.011797 0.023752 0.033385 0.020321
Cell Periphery 0.000182 0.000455 0.003590 0.000968 0.000269 0.000256 0.001213 0.005243 0.000226 0.000320 0.002156 0.003001
Cytoplasm 0.833223 0.822107 0.725891 0.746191 0.888774 0.833272 0.544673 0.876914 0.860928 0.829707 0.616574 0.808355
Cytoplasmic Foci 0.092970 0.073092 0.046366 0.030297 0.059861 0.095499 0.050940 0.011886 0.076457 0.088345 0.049125 0.021542
Eisosomes 0.000004 0.000006 0.000008 0.000007 0.000002 0.000006 0.000009 0.000006 0.000003 0.000006 0.000009 0.000007
Endoplasmic Reticulum 0.005886 0.003483 0.003591 0.001225 0.003551 0.002386 0.005980 0.000649 0.004722 0.002736 0.005032 0.000951
Endosome 0.004408 0.008046 0.011957 0.010522 0.002344 0.004927 0.017349 0.001982 0.003379 0.005923 0.015209 0.006461
Golgi 0.009786 0.010596 0.017856 0.013381 0.009902 0.014439 0.014309 0.006468 0.009844 0.013212 0.015716 0.010093
Lipid Particles 0.000871 0.002193 0.018408 0.007932 0.000606 0.000786 0.016209 0.011283 0.000738 0.001235 0.017082 0.009525
Mitochondria 0.000478 0.000114 0.000033 0.001701 0.000385 0.000601 0.000533 0.001314 0.000431 0.000445 0.000335 0.001517
Mitotic Spindle 0.000530 0.000763 0.006147 0.004813 0.000446 0.004082 0.002957 0.001679 0.000488 0.003022 0.004223 0.003323
None 0.001777 0.002362 0.000743 0.000221 0.001196 0.000612 0.000666 0.000282 0.001487 0.001170 0.000697 0.000250
Nuclear Periphery 0.000275 0.000132 0.000220 0.000094 0.000034 0.000020 0.000346 0.000030 0.000155 0.000056 0.000296 0.000064
Nuclear Periphery Foci 0.000878 0.001469 0.015410 0.001567 0.000992 0.000339 0.007087 0.002171 0.000935 0.000700 0.010389 0.001854
Nucleolus 0.000108 0.000615 0.000047 0.000208 0.000052 0.000078 0.000156 0.000090 0.000080 0.000249 0.000113 0.000152
Nucleus 0.004906 0.006243 0.010644 0.003740 0.002704 0.001907 0.005228 0.003467 0.003808 0.003292 0.007377 0.003610
Peroxisomes 0.000198 0.000802 0.000266 0.000856 0.000120 0.000459 0.000546 0.000403 0.000159 0.000568 0.000435 0.000640
Vacuole 0.015991 0.029239 0.102523 0.142713 0.008542 0.012804 0.273550 0.052446 0.012276 0.018051 0.205692 0.099788
Vacuole Periphery 0.013449 0.007345 0.004750 0.009303 0.009925 0.005825 0.015280 0.002811 0.011691 0.006310 0.011102 0.006216

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.79 -7.12 -3.51 -2.24 1.91 -3.64 -4.11 -5.62 -3.05 1.76 -5.34 -6.68 -5.49 -3.33 2.33
Bud Neck -4.54 -9.58 -13.84 -8.37 6.51 -6.85 -7.51 -12.35 -9.07 4.70 -7.18 -11.75 -18.51 -13.43 7.51
Bud Site -6.69 -4.43 -4.31 3.05 1.36 -7.70 -6.74 -7.12 1.06 4.47 -9.52 -7.74 -7.58 2.28 4.49
Cell Periphery -4.15 -8.94 -9.25 -4.42 6.92 0.28 -4.79 -7.31 -7.33 -5.72 -2.63 -9.85 -8.34 -8.02 -2.10
Cytoplasm 1.06 7.46 10.31 6.86 -1.32 7.21 23.43 2.47 -4.77 -21.93 4.93 22.03 9.96 3.90 -16.05
Cytoplasmic Foci 3.41 9.32 16.47 8.76 5.14 -7.33 2.27 16.93 19.97 10.32 -3.07 8.16 22.79 20.28 10.44
Eisosomes -1.52 -4.33 -2.67 -0.59 1.35 -4.61 -6.71 -6.00 -0.03 3.41 -3.80 -7.59 -5.05 -0.68 3.28
Endoplasmic Reticulum 2.12 2.19 5.94 2.64 2.84 1.16 -1.90 4.60 1.96 5.50 2.53 0.04 7.47 2.78 6.05
Endosome -3.00 -6.16 -4.22 -0.91 1.64 -4.93 -10.22 0.86 4.85 10.24 -4.59 -11.58 -3.82 -0.03 7.85
Golgi -0.54 -4.63 -1.05 -0.44 2.50 -3.20 -1.30 2.96 4.74 2.66 -3.13 -3.20 0.68 2.83 3.10
Lipid Particles -1.91 -10.37 -9.59 -5.99 5.90 -2.01 -8.20 -11.24 -11.04 2.66 -2.05 -12.21 -14.78 -13.34 5.53
Mitochondria 4.16 5.30 -1.87 -2.60 -2.76 -1.15 -0.27 -0.96 -0.70 -0.83 -0.11 0.74 -1.82 -1.75 -1.98
Mitotic Spindle -0.89 -6.73 -3.42 -3.21 1.21 -3.01 -3.81 -1.61 1.75 1.36 -3.05 -7.18 -3.78 -0.12 1.28
None -0.35 2.45 4.30 1.26 1.89 1.81 2.39 5.93 1.14 1.69 0.50 3.39 6.27 1.60 2.55
Nuclear Periphery 0.74 0.22 1.03 0.45 4.20 1.11 -3.09 0.28 -1.08 3.13 1.08 -1.35 1.03 -0.27 3.81
Nuclear Periphery Foci -1.91 -7.65 -3.45 -0.73 7.20 4.25 -5.81 -5.79 -11.35 4.65 1.46 -9.51 -6.30 -8.29 8.46
Nucleolus -1.17 3.26 -3.23 0.97 -5.88 -1.06 -5.10 -3.44 -0.45 3.16 -1.22 -2.46 -4.22 0.76 -1.72
Nucleus -0.66 -4.16 0.41 0.80 4.51 3.28 -3.94 -3.17 -5.89 2.59 0.62 -5.30 -1.20 -1.25 4.93
Peroxisomes -2.72 -1.10 -6.54 0.12 -4.75 -6.05 -6.50 -6.62 0.84 2.10 -4.97 -5.82 -8.58 -0.35 -2.30
Vacuole -3.69 -9.05 -24.05 -19.07 -4.02 -3.55 -21.51 -16.35 -14.61 17.36 -4.00 -21.90 -27.44 -24.67 10.65
Vacuole Periphery 3.24 6.50 3.61 -0.51 -3.16 3.34 -1.93 6.98 3.24 6.17 5.18 1.24 6.76 0.77 3.73
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Component of RQC, which mediates nascent chain degradation; RQC (ribosome quality control complex) is a ribosome-bound complex required for degradation of polypeptides arising from stalled translation; recruits alanine- and threonine-charged tRNA to the A site and directs the elongation of nascent chains independently of mRNA or 40S subunits; monitors translation stress and signals this to Hsf1p
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Rqc2

Rqc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rqc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available