Standard name
Human Ortholog
Description Single-stranded DNA endonuclease (with Rad10p); cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.09 0.07 0.07 0.06 0.05 0.06 0 0.06 0.06 0.12 0.14 0.14 0.1 0.11 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.19 0.54 0.67 0.51 0.75 0.72 0.72 0.81 0.75 0.79 0.84 0.12 0 0.2 0.05 0 0.08 0 0 0 0 0 0
Nucleus 0.92 0.88 0.77 0.74 0.71 0.67 0.7 0.68 0.63 0.67 0.64 0.65 0.83 0.81 0.72 0.76 0.77 0.77 0.88 0.84 0.79 0.88 0.82 0.56
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.12 0.13 0.13 0 0.09 0.16 0.08 0.14 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0
Bud 0 0 1 2 3 2 3 6 3 12 4 12 0 0 0 0 0 0 3 1 0 0 0 2
Bud Neck 0 0 0 0 0 1 1 1 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0
Bud Site 0 0 0 3 5 9 17 23 14 14 9 23 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 4 12 19 21 26 27 28 20 18 20 21 9 17 18 21 21 12 1 0 2 1 0 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 11 7 4 0 0 0 0 0 1
Endosome 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 6 2 3 1 2 0 0 0 3
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 2 2 0 0 0 0 0 0
Mitochondria 0 46 145 149 158 259 341 377 251 303 245 314 9 3 26 11 6 11 0 6 2 0 0 4
Nucleus 146 211 207 164 222 234 332 357 197 272 198 245 62 97 94 156 147 105 148 218 219 61 120 102
Nuclear Periphery 0 1 0 0 2 2 1 4 0 4 6 7 0 1 1 0 0 0 1 0 0 0 0 0
Nucleolus 2 2 0 1 4 1 3 9 4 3 3 8 1 1 2 0 1 0 2 1 0 0 3 9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 2 1 5 9 1 4 0 3 0 1 0 3 1 1 2 1 6 1 0 0
Vac/Vac Membrane 7 13 4 1 8 3 2 3 2 0 3 2 1 5 11 25 25 18 6 23 44 5 20 53
Unique Cell Count 158 240 268 223 311 347 476 527 311 405 310 376 75 120 131 205 191 136 169 261 279 70 148 182
Labelled Cell Count 161 277 371 342 426 539 732 817 492 631 488 635 82 126 152 235 213 156 169 261 279 70 148 182


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 4.7 3.5 3.5 3.6 3.5 3.2 3.3 3.0 3.0 3.1 2.8 4.9 5.1 4.3 6.4 6.8 7.3 4.8 5.5 6.0
Std Deviation (1e-4) 1.0 0.9 0.6 1.1 1.0 1.6 0.9 0.9 1.2 1.0 1.2 1.0 1.0 0.9 0.9 1.5 1.8 2.7 0.7 1.6 1.4
Intensity Change (Log2) – – – -0.01 0.03 -0.01 -0.15 -0.11 -0.25 -0.26 -0.21 -0.35 0.47 0.53 0.28 0.85 0.94 1.05 0.43 0.64 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.8 5.0 4.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0533 1.5909 1.4033 1.0977 1.1303 1.6071 1.5369 2.7322 2.2105 1.2903 1.5582 2.1541 2.9531 3.3826 3.2057 3.5345 2.4563 3.1055
Actin 0.0397 0 0.0069 0.0037 0.0123 0.0098 0.0262 0.0003 0.0054 0 0.0024 0.0001 0.0095 0 0.0009 0.021 0.0005 0.0045
Bud 0.0006 0 0.0001 0.0008 0.0001 0.0001 0.0002 0.0003 0.0001 0 0.0005 0.0001 0.0004 0 0.0002 0.0003 0 0.0003
Bud Neck 0.0139 0.0002 0.0004 0.003 0.0013 0.0017 0.0008 0.0019 0.0004 0.0002 0.0006 0.0018 0.0013 0.0001 0.001 0.0019 0.0005 0.002
Bud Periphery 0.0013 0 0.0001 0.0025 0.0003 0.0004 0.0004 0.0005 0.0001 0 0.0009 0.0001 0.0013 0 0.0003 0.0005 0 0.0007
Bud Site 0.015 0.0001 0.0002 0.0038 0.0003 0.0002 0.0015 0.0069 0.0002 0.0001 0.0007 0.0001 0.0011 0.0001 0.0069 0.0021 0.0001 0.0002
Cell Periphery 0.0009 0 0 0.0004 0.0001 0.0001 0.0002 0.0003 0.0001 0 0.0001 0 0.0002 0 0.0004 0.0003 0 0.0001
Cytoplasm 0.0059 0.0037 0.0004 0.0004 0.0009 0.002 0.0134 0.0037 0.0196 0.0018 0.0037 0.0059 0.003 0.0023 0.006 0.0019 0.0071 0.0009
Cytoplasmic Foci 0.009 0.0001 0.0008 0.003 0.0022 0.0025 0.0035 0.0003 0.0021 0 0.0039 0.0002 0.0016 0 0.0008 0.0059 0.0001 0.0002
Eisosomes 0.0008 0 0 0.0001 0.0003 0.0001 0.0005 0 0.0001 0 0 0 0.0001 0 0 0.0011 0 0.0001
Endoplasmic Reticulum 0.0049 0.0004 0.0003 0.0001 0.0023 0.0007 0.003 0.0002 0.0022 0 0.0021 0.0012 0.0026 0.0004 0.0008 0.0014 0.0004 0.0002
Endosome 0.0233 0.0013 0.0044 0.0039 0.0115 0.0024 0.0045 0.0005 0.0044 0 0.0371 0.0017 0.009 0.0003 0.0013 0.0476 0.0006 0.0025
Golgi 0.0068 0 0.0018 0.0035 0.0077 0.0022 0.0028 0.0002 0.0009 0 0.0098 0 0.0034 0 0.0002 0.0119 0 0.0013
Lipid Particles 0.0268 0 0.0024 0.0031 0.0149 0.0005 0.0025 0.0002 0.0008 0 0.004 0 0.0025 0 0.0003 0.0122 0 0.0016
Mitochondria 0.0076 0.0002 0.0014 0.0101 0.0394 0.0026 0.0026 0.0006 0.0006 0.0002 0.0073 0.0008 0.0059 0.0001 0.0005 0.0055 0.0003 0.0063
None 0.0109 0.0003 0.0001 0.0001 0.0018 0.0004 0.0609 0.0152 0.0408 0.1651 0.0007 0.0179 0.0144 0.0002 0.0046 0.0019 0.0098 0.0191
Nuclear Periphery 0.0467 0.0214 0.0024 0.0009 0.0191 0.0042 0.0272 0.0081 0.0156 0.0028 0.0145 0.0033 0.0108 0.0084 0.0098 0.0028 0.0141 0.001
Nucleolus 0.008 0.0059 0.0049 0.0094 0.0077 0.0122 0.0066 0.0055 0.0049 0.005 0.0027 0.0134 0.0093 0.0022 0.0072 0.0094 0.0062 0.0091
Nucleus 0.7262 0.9539 0.9709 0.9394 0.8694 0.9522 0.8138 0.9521 0.8969 0.7934 0.8994 0.9352 0.9092 0.9832 0.9523 0.8411 0.9505 0.9448
Peroxisomes 0.0095 0 0.0007 0.0058 0.0022 0.0001 0.0061 0.0002 0.0002 0 0.0013 0 0.0006 0 0.0004 0.0213 0 0.0001
Punctate Nuclear 0.0346 0.0109 0.0013 0.0026 0.0042 0.0043 0.0221 0.0019 0.0039 0.0313 0.0051 0.0159 0.0112 0.0018 0.0034 0.003 0.0092 0.0043
Vacuole 0.004 0.0011 0.0003 0.0028 0.0008 0.0008 0.0006 0.0008 0.0006 0.0001 0.0018 0.0017 0.0016 0.0006 0.0025 0.0051 0.0003 0.0004
Vacuole Periphery 0.0038 0.0003 0.0002 0.0006 0.0011 0.0005 0.0007 0.0001 0.0004 0 0.0014 0.0005 0.001 0.0001 0.0002 0.002 0.0001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.6987 11.9452 18.8357 18.8478 18.8409 15.0244 18.4941 18.8508 21.0628 23.0174
Translational Efficiency 0.3666 0.39 0.2835 0.3045 0.2671 0.3672 0.2483 0.252 0.2353 0.1985

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2284 999 1766 1334 1776 1988 73 1344 4060 2987 1839 2678

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 665.01 781.63 1050.84 848.30 664.17 748.50 1009.81 843.99 664.64 759.58 1049.21 846.14
Standard Deviation 72.51 103.48 128.93 114.20 105.04 100.08 115.46 116.19 88.23 102.43 128.67 115.23
Intensity Change Log 2 — 0.233110 0.660095 0.351199 — 0.172450 0.604459 0.345673 — 0.203118 0.632563 0.348440

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000028 0.000258 0.000280 0.001231 0.000096 0.000186 0.000638 0.001366 0.000058 0.000210 0.000294 0.001298
Bud Neck 0.002153 0.003716 0.005392 0.023174 0.002988 0.007540 0.001963 0.010480 0.002518 0.006261 0.005256 0.016803
Bud Site 0.000555 0.001967 0.002219 0.023373 0.000716 0.004054 0.000459 0.015830 0.000626 0.003356 0.002149 0.019588
Cell Periphery 0.000102 0.000106 0.000070 0.000214 0.000148 0.000147 0.000063 0.000161 0.000122 0.000133 0.000069 0.000188
Cytoplasm 0.062199 0.027423 0.027569 0.084574 0.051852 0.027439 0.004719 0.074730 0.057673 0.027434 0.026662 0.079634
Cytoplasmic Foci 0.005569 0.003891 0.000217 0.007653 0.003735 0.005622 0.000530 0.007994 0.004767 0.005043 0.000229 0.007824
Eisosomes 0.000012 0.000023 0.000011 0.000036 0.000019 0.000020 0.000040 0.000034 0.000015 0.000021 0.000012 0.000035
Endoplasmic Reticulum 0.000774 0.003569 0.003703 0.007229 0.001613 0.003439 0.009665 0.006915 0.001141 0.003482 0.003939 0.007071
Endosome 0.000300 0.000511 0.000310 0.008450 0.000450 0.001988 0.000360 0.009624 0.000366 0.001494 0.000312 0.009039
Golgi 0.000134 0.000169 0.000033 0.004825 0.000305 0.001098 0.000044 0.006557 0.000209 0.000788 0.000033 0.005694
Lipid Particles 0.000617 0.001666 0.000107 0.003246 0.001047 0.001163 0.000594 0.003561 0.000805 0.001331 0.000127 0.003404
Mitochondria 0.000832 0.002364 0.000684 0.008928 0.001378 0.005144 0.000614 0.008955 0.001071 0.004214 0.000681 0.008942
Mitotic Spindle 0.000054 0.003233 0.000227 0.066069 0.000554 0.004698 0.000045 0.039516 0.000272 0.004208 0.000220 0.052743
None 0.011020 0.006938 0.002470 0.012065 0.022023 0.004059 0.001157 0.008669 0.015833 0.005022 0.002418 0.010360
Nuclear Periphery 0.000687 0.000830 0.000679 0.003812 0.001212 0.002136 0.000910 0.003039 0.000917 0.001699 0.000688 0.003424
Nuclear Periphery Foci 0.000275 0.000515 0.000241 0.001049 0.000600 0.001258 0.000782 0.001633 0.000417 0.001009 0.000262 0.001342
Nucleolus 0.005175 0.006951 0.000405 0.002784 0.009125 0.005822 0.002947 0.002859 0.006903 0.006200 0.000506 0.002822
Nucleus 0.907402* 0.932817* 0.949925* 0.699790* 0.900430* 0.913984* 0.967043* 0.761408* 0.904352* 0.920283* 0.950605* 0.730714*
Peroxisomes 0.000462 0.001191 0.000110 0.002417 0.000439 0.002172 0.000221 0.001649 0.000452 0.001844 0.000115 0.002032
Vacuole 0.001573 0.001694 0.005292 0.034259 0.001043 0.006625 0.007121 0.027161 0.001341 0.004976 0.005364 0.030697
Vacuole Periphery 0.000076 0.000167 0.000057 0.004823 0.000226 0.001407 0.000087 0.007860 0.000142 0.000992 0.000058 0.006347

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.60 -17.14 -12.37 -8.55 -8.61 -2.98 -3.86 -3.95 -3.55 0.34 -7.11 -14.31 -7.94 -6.57 -5.41
Bud Neck -2.49 -5.43 -11.59 -9.88 -8.92 -5.58 -0.09 -11.96 -5.27 -9.13 -6.40 -5.47 -15.85 -10.32 -10.82
Bud Site -2.18 -2.77 -8.04 -6.63 -6.70 -4.11 -0.98 -5.73 -3.25 -5.42 -4.75 -2.71 -9.79 -6.64 -7.65
Cell Periphery -0.42 3.38 -3.47 -2.02 -6.48 0.05 2.57 0.97 0.53 -2.12 -0.60 5.61 -1.74 -0.32 -8.08
Cytoplasm 8.63 11.63 -1.48 -8.88 -10.99 6.95 11.37 -2.76 -8.95 -12.70 11.83 13.12 -2.84 -12.97 -14.02
Cytoplasmic Foci 1.61 7.38 3.55 1.51 -5.45 -2.25 5.42 0.31 2.32 -4.04 -0.34 9.55 2.98 3.01 -7.05
Eisosomes -4.89 0.48 -18.19 -9.48 -18.88 -0.30 -3.39 -6.10 -10.70 1.28 -2.82 0.57 -15.04 -15.01 -21.93
Endoplasmic Reticulum -11.86 -16.71 -22.51 -14.62 -13.12 -5.91 -5.11 -17.75 -12.06 1.75 -11.10 -15.59 -28.72 -18.40 -14.10
Endosome -2.26 -0.09 -6.58 -6.02 -6.54 -4.08 -1.23 -6.00 -3.10 -4.78 -4.46 0.25 -8.75 -5.00 -8.71
Golgi -0.64 1.30 -2.08 -2.00 -2.29 -1.87 1.28 -3.01 -1.77 -3.45 -2.16 2.00 -3.67 -2.23 -4.14
Lipid Particles -1.47 7.48 -1.98 0.11 -3.07 -0.66 -0.06 -1.38 -1.08 -1.26 -1.99 5.47 -2.44 -0.73 -4.42
Mitochondria -1.48 0.34 -5.90 -1.93 -6.37 -4.34 1.41 -3.36 0.25 -4.22 -4.82 1.46 -6.11 -0.65 -7.06
Mitotic Spindle -2.20 -2.71 -10.26 -8.76 -10.12 -3.63 0.70 -7.71 -6.08 -7.78 -4.40 -1.27 -12.81 -10.58 -12.69
None 2.72 8.57 2.58 -0.52 -6.72 8.28 9.94 6.87 -2.17 -5.85 9.06 12.32 7.14 -2.52 -7.57
Nuclear Periphery -1.46 -2.95 -11.18 -10.70 -10.69 -2.23 -0.46 -7.57 -4.99 -5.59 -3.20 -0.08 -13.63 -9.31 -14.70
Nuclear Periphery Foci -1.57 -0.27 -4.90 -2.75 -4.42 -2.18 -1.13 -2.62 0.18 0.78 -2.70 -0.31 -5.13 -0.69 -4.04
Nucleolus -0.77 6.37 3.05 3.29 -5.73 2.22 4.61 4.79 3.14 -0.34 0.95 9.63 5.54 4.59 -6.45
Nucleus -3.53 -9.06 17.74 18.76 23.77 -1.76 -3.14 11.69 13.24 9.66 -2.99 -11.40 20.92 22.19 29.29
Peroxisomes -2.30 3.16 -0.92 1.66 -3.05 -3.40 0.68 -0.81 2.73 -1.22 -3.97 3.75 -1.18 3.18 -3.53
Vacuole -0.61 -7.37 -16.32 -16.32 -14.12 -6.44 -2.68 -14.72 -12.34 -4.14 -5.91 -8.53 -21.88 -19.82 -17.65
Vacuole Periphery -4.21 1.14 -3.05 -2.84 -3.08 -3.69 1.12 -3.12 -1.77 -3.23 -4.07 2.25 -4.20 -2.41 -4.36
Standard name
Human Ortholog
Description Single-stranded DNA endonuclease (with Rad10p); cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rad1

Rad1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available