Standard name
Human Ortholog
Description Zinc-finger DNA-binding transcription factor; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; involved in transcriptional regulation of the methionine biosynthetic genes; feedforward loop controlling expression of MET32 and the lack of such a loop for MET31 may account for the differential actions of Met31p and Met32p; MET31 has a paralog, MET32, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.37 0.3 0.46 0.5 0.66 0.61 0.7 0.69 0.67 0.72 0.5 0.59 0.38 0.36 0.43 0.15 0.14 0.16 0.28 0.21 0.18 0.23 0.24 0.2
Endoplasmic Reticulum 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0.09 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.39 0.12 0 0.09 0.05 0.41 0.17 0.25 0.34 0.32 0.58 0.41 0.06 0 0 0.05 0.08 0.06 0.1 0 0.14 0.06 0.05 0.05
Nucleus 0.18 0.48 0.3 0.26 0.16 0.09 0.08 0.05 0 0 0.05 0.07 0.49 0.51 0.4 0.73 0.68 0.72 0.23 0.39 0.28 0.36 0.34 0.33
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0.08
Vac/Vac Membrane 0.18 0.2 0.23 0.12 0.15 0 0.1 0 0 0 0.06 0 0.09 0.11 0.14 0.11 0.13 0.1 0.24 0.25 0.18 0.19 0.2 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0
Bud 0 0 0 1 0 2 2 3 1 2 2 1 0 0 0 1 0 0 0 3 2 8 12 18
Bud Neck 0 0 2 0 2 2 2 9 1 3 0 0 0 0 1 0 0 0 1 0 0 1 3 7
Bud Site 0 0 0 0 0 5 0 3 0 0 0 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 14 48 28 89 132 117 229 353 91 141 79 98 151 194 314 54 35 42 28 45 17 92 150 130
Endoplasmic Reticulum 3 0 1 0 0 0 0 0 0 0 0 0 2 0 1 21 12 11 0 0 1 1 3 2
Endosome 0 0 1 16 11 2 7 10 2 1 1 2 9 24 30 8 10 6 2 3 2 5 10 14
Golgi 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 3 0 1 1 1 0 0 1
Mitochondria 15 20 0 17 9 79 54 128 46 63 91 68 23 13 18 19 21 16 10 8 13 22 32 35
Nucleus 7 78 18 46 32 18 25 25 6 5 8 12 195 273 293 254 169 187 23 82 27 146 213 220
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1 1
Nucleolus 0 0 0 0 0 0 0 4 1 0 2 2 0 0 0 0 1 0 0 1 0 12 14 15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 4 5 1 11 8 1 8 5 4 4 2 2 1 2 1 3 9 5 27 42 49
Vac/Vac Membrane 7 33 14 22 30 2 34 22 6 6 10 6 36 61 102 40 33 26 24 52 17 76 125 142
Unique Cell Count 38 161 61 179 199 191 327 508 135 197 157 166 399 537 729 349 250 260 103 212 98 408 624 658
Labelled Cell Count 46 179 66 195 221 228 364 566 155 229 198 198 421 567 761 400 286 289 103 212 98 408 624 658


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.2 5.1 5.2 5.5 3.6 4.3 4.2 3.9 4.1 3.9 4.3 4.6 4.7 4.7 6.1 6.1 6.3 4.1 4.2 4.2
Std Deviation (1e-4) 0.5 0.6 0.9 1.7 1.9 1.4 1.2 1.4 1.5 1.7 1.6 2.2 1.0 1.1 0.9 1.4 1.5 1.4 0.8 1.1 1.1
Intensity Change (Log2) – – – 0.03 0.12 -0.48 -0.23 -0.27 -0.37 -0.3 -0.36 -0.24 -0.12 -0.1 -0.09 0.28 0.27 0.31 -0.31 -0.27 -0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -2.4 -1.9 -2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.0405 -0.1291 -0.2738 -0.619 -0.7225 -0.37 0.5473 0.7786 0.796 0.4467 0.6323 0.6827 3.1902 2.612 2.4744 2.4275 2.6875 2.7622
Actin 0.0112 0.0003 0.0002 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0381 0.0009 0.002 0.006 0.0044 0.0097
Bud 0.0003 0.0001 0 0 0 0 0 0 0 0 0 0 0.0004 0.0013 0.0002 0.0005 0.0001 0.0001
Bud Neck 0.0043 0.0007 0.0004 0.0004 0.0008 0.0017 0.0004 0.0003 0.0002 0.0005 0.0004 0.0013 0.0085 0.001 0.0012 0.0067 0.0072 0.0023
Bud Periphery 0.0007 0.0001 0 0 0 0 0.0001 0 0 0 0 0 0.0007 0.0006 0.0003 0.0019 0.0001 0.0001
Bud Site 0.001 0.0009 0.0001 0.0001 0.0001 0 0.0001 0.0004 0.0002 0 0.0001 0.0001 0.002 0.0034 0.0018 0.0039 0.0007 0.0002
Cell Periphery 0.0001 0 0 0 0 0 0 0 0 0 0 0 0.0002 0.0001 0.0001 0.0031 0 0
Cytoplasm 0.0257 0.0481 0.0229 0.0318 0.0252 0.0083 0.0175 0.03 0.0103 0.0249 0.0184 0.0115 0.0458 0.0676 0.0459 0.0565 0.0246 0.0253
Cytoplasmic Foci 0.0038 0.001 0.0006 0.0009 0.0009 0.0004 0.0006 0.0008 0.0002 0.0009 0.0003 0.0018 0.0064 0.0045 0.0063 0.0089 0.0161 0.0032
Eisosomes 0.0002 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0.0003 0.0002 0.0002 0.0006 0.0001 0.0002
Endoplasmic Reticulum 0.0037 0.0022 0.0009 0.0008 0.0006 0.0003 0.0005 0.0008 0.0002 0.0004 0.0005 0.0002 0.0031 0.0028 0.0011 0.0013 0.001 0.0009
Endosome 0.0065 0.0021 0.001 0.0014 0.0012 0.0004 0.0013 0.0014 0.0003 0.0007 0.0006 0.0022 0.0106 0.0031 0.0055 0.005 0.0046 0.004
Golgi 0.0008 0.0001 0 0 0 0 0 0 0 0 0 0.0001 0.0053 0.0002 0.0019 0.0029 0.0023 0.0016
Lipid Particles 0.0035 0.0012 0.0002 0.0001 0.0001 0 0.0001 0.0002 0 0.0001 0.0001 0.0004 0.0038 0.0005 0.0043 0.0082 0.005 0.0022
Mitochondria 0.0031 0.0047 0.0003 0.0004 0.0004 0.0001 0.0005 0.0003 0.0002 0.0002 0.0004 0.0001 0.0017 0.0009 0.0015 0.0103 0.0005 0.0008
None 0.056 0.0922 0.0781 0.0332 0.101 0.0332 0.0597 0.0536 0.0244 0.033 0.0614 0.0185 0.0679 0.2175 0.1195 0.0753 0.2065 0.0495
Nuclear Periphery 0.0246 0.0288 0.0173 0.0102 0.0333 0.0059 0.0086 0.0126 0.0062 0.0074 0.0143 0.0034 0.0166 0.0136 0.0104 0.0193 0.0086 0.0062
Nucleolus 0.009 0.0081 0.0076 0.0082 0.019 0.0122 0.0072 0.0063 0.0055 0.0049 0.0079 0.0132 0.0127 0.0097 0.0092 0.009 0.0154 0.0152
Nucleus 0.7919 0.7515 0.8281 0.8105 0.7434 0.8842 0.8773 0.8607 0.9271 0.8998 0.8738 0.9067 0.74 0.6257 0.7248 0.7022 0.6113 0.8279
Peroxisomes 0.0013 0.0002 0 0 0 0 0.0001 0 0 0 0 0.0001 0.0028 0.0017 0.0058 0.0125 0.0038 0.0069
Punctate Nuclear 0.0491 0.0554 0.0413 0.1012 0.0731 0.0524 0.025 0.0316 0.0249 0.0265 0.0208 0.0399 0.0305 0.0422 0.0473 0.0616 0.086 0.0428
Vacuole 0.002 0.0016 0.0006 0.0003 0.0005 0.0002 0.0004 0.0008 0.0002 0.0004 0.0004 0.0003 0.0018 0.0022 0.0098 0.0032 0.0014 0.0006
Vacuole Periphery 0.001 0.0006 0.0002 0.0002 0.0003 0.0001 0.0002 0.0002 0.0001 0.0002 0.0003 0.0001 0.0008 0.0003 0.0007 0.001 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.5304 35.0958 19.1893 16.7075 17.4138 6.1955 19.6103 17.6475 14.1468 18.895
Translational Efficiency 0.9439 0.5649 0.9112 0.9488 0.9628 2.6206 0.7907 1.2553 1.3563 0.8336

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1176 1035 161 549 1746 1734 1118 1544 2922 2769 1279 2093

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 638.30 707.04 893.87 833.46 655.02 766.14 841.57 825.24 648.29 744.05 848.15 827.40
Standard Deviation 75.97 109.49 99.00 96.94 69.61 90.26 99.36 102.27 72.70 101.98 100.82 100.96
Intensity Change Log 2 — 0.147557 0.485830 0.384878 — 0.226069 0.361544 0.333275 — 0.187854 0.424222 0.358974

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000077 0.000358 0.000999 0.000423 0.000130 0.000230 0.000437 0.000661 0.000109 0.000278 0.000508 0.000598
Bud Neck 0.006458 0.019166 0.004013 0.002740 0.003210 0.013467 0.003711 0.009140 0.004517 0.015597 0.003749 0.007462
Bud Site 0.000780 0.007488 0.001183 0.006497 0.000818 0.006607 0.002594 0.007255 0.000803 0.006936 0.002417 0.007056
Cell Periphery 0.000160 0.000186 0.000125 0.000202 0.000129 0.000253 0.000116 0.000147 0.000141 0.000228 0.000117 0.000162
Cytoplasm 0.293996* 0.324513* 0.009706 0.221533* 0.317784* 0.268518* 0.105330* 0.225442* 0.308210* 0.289448* 0.093293 0.224417*
Cytoplasmic Foci 0.135060 0.106343 0.000407 0.018023 0.068790 0.055574 0.002629 0.005048 0.095462 0.074550 0.002349 0.008451
Eisosomes 0.000112 0.000079 0.000052 0.000080 0.000053 0.000056 0.000031 0.000027 0.000077 0.000064 0.000033 0.000040
Endoplasmic Reticulum 0.000791 0.002834 0.010370 0.003268 0.000741 0.001151 0.004791 0.004044 0.000761 0.001780 0.005493 0.003840
Endosome 0.002913 0.008175 0.000618 0.002842 0.001467 0.004841 0.001076 0.001438 0.002049 0.006088 0.001018 0.001806
Golgi 0.000746 0.003238 0.000071 0.000094 0.000316 0.001789 0.000063 0.000575 0.000489 0.002331 0.000064 0.000449
Lipid Particles 0.015294 0.007825 0.000789 0.002330 0.006930 0.006037 0.000846 0.000688 0.010296 0.006705 0.000839 0.001119
Mitochondria 0.006533 0.008898 0.000719 0.000881 0.003380 0.008091 0.000835 0.001340 0.004649 0.008393 0.000820 0.001220
Mitotic Spindle 0.000279 0.005192 0.000202 0.005020 0.002501 0.005745 0.001013 0.006146 0.001607 0.005538 0.000911 0.005851
None 0.010011 0.012747 0.003425 0.013957 0.008662 0.009239 0.008534 0.009252 0.009205 0.010550 0.007891 0.010486
Nuclear Periphery 0.001182 0.002057 0.001011 0.001816 0.001527 0.001880 0.001409 0.001898 0.001388 0.001946 0.001359 0.001876
Nuclear Periphery Foci 0.000387 0.001302 0.000404 0.000989 0.000842 0.000517 0.000754 0.000631 0.000659 0.000811 0.000710 0.000725
Nucleolus 0.003410 0.008088 0.002637 0.001585 0.002369 0.004482 0.001731 0.001067 0.002788 0.005830 0.001845 0.001203
Nucleus 0.506423* 0.448189* 0.954964* 0.648900* 0.570501* 0.589677* 0.852245* 0.703817* 0.544712* 0.536791* 0.865175* 0.689412*
Peroxisomes 0.006072 0.007339 0.000241 0.000495 0.003511 0.005733 0.000245 0.000381 0.004542 0.006334 0.000245 0.000411
Vacuole 0.008882 0.024233 0.007933 0.067929 0.006015 0.014675 0.011463 0.020788 0.007169 0.018247 0.011018 0.033153
Vacuole Periphery 0.000432 0.001750 0.000132 0.000396 0.000324 0.001437 0.000147 0.000216 0.000368 0.001554 0.000145 0.000263

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.00 -7.76 -3.04 -1.45 2.79 -1.37 -6.80 -2.09 -1.49 0.25 -3.10 -10.93 -2.96 -1.80 0.74
Bud Neck -5.58 0.82 2.15 7.57 1.92 -7.61 -2.28 -6.57 2.25 -4.62 -9.18 -0.58 -4.43 5.62 -3.94
Bud Site -5.73 -3.48 -3.25 0.70 -2.59 -5.73 -2.21 -5.53 0.09 -2.92 -7.96 -2.41 -6.43 0.48 -3.39
Cell Periphery -1.30 1.18 -2.04 -0.22 -2.17 -2.58 1.11 -0.15 2.48 -0.96 -2.78 2.60 -0.44 2.52 -2.31
Cytoplasm -2.61 37.59 9.25 11.02 -17.26 5.58 28.11 13.58 8.07 -13.73 2.76 34.69 16.16 13.17 -16.63
Cytoplasmic Foci 3.52 25.56 21.66 16.86 -8.83 3.38 23.09 21.94 19.44 -4.31 5.22 33.34 30.83 25.60 -8.36
Eisosomes 4.21 5.95 1.03 -3.06 -4.93 -1.11 3.28 6.14 7.26 2.44 3.08 9.20 6.89 3.94 -2.82
Endoplasmic Reticulum -8.88 -9.86 -12.43 -6.62 5.44 -2.62 -16.24 -15.00 -13.74 3.31 -7.85 -18.97 -19.18 -14.28 5.02
Endosome -6.91 6.43 -0.83 6.61 -8.23 -6.59 1.24 0.51 6.60 -0.64 -9.38 4.13 0.99 9.74 -3.02
Golgi -3.13 6.34 6.31 3.98 -0.37 -3.64 4.56 -0.13 3.09 -1.19 -4.70 7.91 1.14 4.82 -1.22
Lipid Particles 5.00 11.02 9.33 7.21 -5.75 1.04 8.54 8.76 8.80 1.52 4.37 13.87 13.22 12.01 -3.53
Mitochondria -1.40 4.32 4.13 6.75 -0.71 -4.12 4.04 3.50 7.07 -1.99 -3.85 5.83 5.36 9.66 -1.98
Mitotic Spindle -3.55 -1.37 -2.93 0.03 -2.50 -2.17 0.44 -3.54 -1.72 -4.07 -3.74 -0.48 -4.60 -1.66 -4.52
None -1.45 6.01 0.38 1.30 -2.71 -0.47 1.43 1.03 1.33 -0.32 -1.23 2.96 1.24 2.17 -1.26
Nuclear Periphery -4.25 -1.61 -9.07 -2.88 -4.56 -1.25 -1.96 -4.37 -2.87 -4.24 -2.93 -3.50 -7.77 -3.82 -5.77
Nuclear Periphery Foci -4.20 -1.74 -6.08 -0.06 -1.03 1.10 -1.22 -0.27 -2.60 1.47 -0.70 -2.19 -1.97 -1.60 0.98
Nucleolus -5.61 0.94 6.01 8.90 2.66 -3.61 1.75 5.81 6.54 2.96 -6.34 2.92 8.47 10.82 3.52
Nucleus 4.50 -41.28 -8.15 -11.62 21.38 -1.77 -29.08 -13.05 -10.95 15.07 1.06 -39.71 -16.56 -16.99 20.47
Peroxisomes -1.48 9.21 8.45 8.78 -1.77 -2.07 5.96 5.78 6.46 -0.66 -2.44 10.30 9.85 10.02 -1.52
Vacuole -8.87 -1.96 -13.37 -11.17 -12.58 -7.16 -8.39 -13.48 -9.74 -5.80 -11.15 -8.19 -18.28 -13.99 -11.67
Vacuole Periphery -5.06 5.22 1.23 5.36 -2.08 -3.55 1.88 1.47 4.22 -1.40 -5.42 3.57 2.29 6.34 -2.46
Standard name
Human Ortholog
Description Zinc-finger DNA-binding transcription factor; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; involved in transcriptional regulation of the methionine biosynthetic genes; feedforward loop controlling expression of MET32 and the lack of such a loop for MET31 may account for the differential actions of Met31p and Met32p; MET31 has a paralog, MET32, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (55%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Met31

Met31


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Met31-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available