Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the HOPS and the CORVET complexes; part of the Class C Vps complex essential for membrane docking and fusion at Golgi-to-endosome and endosome-to-vacuole protein transport stages |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 5 | 2 | 2 | 1 | 2 | 1 | 0 | 1 | – | 0 | 1 | 0 | 3 | 2 | 0 | 1 | 3 |
Bud | 1 | 2 | 11 | 6 | 2 | 13 | 28 | 38 | 16 | 28 | 13 | 27 | 3 | 2 | 2 | – | 1 | 2 | 0 | 1 | 0 | 1 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 3 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 1 | 0 | 1 | 0 | 1 | 1 | 6 | 2 | 3 | 4 | 4 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 9 | 2 | 6 | 12 | 5 | 22 | 23 | 8 | 10 | 9 | 5 | 5 | 11 | 14 | – | 15 | 12 | 2 | 2 | 3 | 1 | 0 | 0 |
Endoplasmic Reticulum | 4 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 4 | 3 | 2 | 2 | 2 | 4 | – | 12 | 16 | 0 | 1 | 1 | 1 | 0 | 0 |
Endosome | 79 | 70 | 120 | 112 | 162 | 175 | 199 | 176 | 42 | 34 | 22 | 59 | 91 | 253 | 266 | – | 232 | 176 | 136 | 180 | 207 | 137 | 261 | 305 |
Golgi | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 28 | 19 | 6 | 4 | 6 | 0 | 2 | 9 |
Mitochondria | 88 | 114 | 72 | 70 | 65 | 143 | 186 | 121 | 182 | 244 | 151 | 157 | 85 | 52 | 64 | – | 40 | 41 | 11 | 13 | 11 | 3 | 5 | 4 |
Nucleus | 2 | 2 | 5 | 2 | 0 | 4 | 5 | 2 | 3 | 0 | 3 | 0 | 1 | 1 | 0 | – | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 12 | 8 | 3 | 0 | 0 | 1 | 2 | 1 | 2 | 3 | 1 | 1 | 6 | 17 | 10 | – | 9 | 24 | 17 | 5 | 11 | 6 | 16 | 11 |
Nucleolus | 54 | 42 | 24 | 22 | 17 | 27 | 29 | 15 | 15 | 24 | 12 | 9 | 49 | 77 | 75 | – | 86 | 59 | 0 | 2 | 1 | 1 | 0 | 2 |
Peroxisomes | 3 | 1 | 8 | 6 | 19 | 23 | 63 | 49 | 9 | 15 | 7 | 16 | 5 | 9 | 22 | – | 1 | 1 | 1 | 1 | 5 | 0 | 2 | 6 |
SpindlePole | 27 | 11 | 81 | 53 | 104 | 87 | 157 | 170 | 54 | 86 | 30 | 82 | 26 | 42 | 35 | – | 73 | 52 | 7 | 5 | 6 | 7 | 11 | 3 |
Vac/Vac Membrane | 43 | 74 | 72 | 38 | 43 | 39 | 31 | 30 | 10 | 16 | 10 | 23 | 56 | 118 | 64 | – | 164 | 140 | 19 | 33 | 29 | 13 | 23 | 22 |
Unique Cell Count | 198 | 233 | 254 | 215 | 301 | 365 | 473 | 437 | 261 | 345 | 200 | 271 | 203 | 375 | 386 | 415 | 326 | 205 | 260 | 291 | 179 | 331 | 374 | |
Labelled Cell Count | 320 | 334 | 402 | 315 | 429 | 521 | 732 | 636 | 348 | 472 | 267 | 384 | 331 | 585 | 557 | 663 | 545 | 205 | 260 | 291 | 179 | 331 | 374 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.5 | 6.0 | 6.3 | 5.7 | 6.6 | 5.2 | 5.4 | 5.7 | 4.9 | 4.6 | 4.5 | 5.1 | 6.4 | 6.8 | 6.7 | – | 10.3 | 9.4 | 5.9 | 6.1 | 6.7 |
Std Deviation (1e-4) | 1.0 | 1.3 | 0.9 | 0.7 | 1.3 | 0.6 | 1.2 | 1.0 | 1.0 | 0.9 | 0.8 | 1.0 | 1.1 | 0.9 | 1.1 | – | 2.1 | 1.7 | 1.1 | 0.9 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.15 | 0.07 | -0.27 | -0.22 | -0.13 | -0.37 | -0.46 | -0.47 | -0.29 | 0.02 | 0.1 | 0.1 | – | 0.71 | 0.58 | -0.09 | -0.06 | 0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud | -0.9 | -2.8 | -0.5 | 0.9 | 2.2 | 0.9 | 0 | 0 | 2.5 | -1.8 | -3.3 | -3.3 | – | 0 | -3.0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cytoplasm | 0 | 2.4 | 0 | 2.8 | 3.0 | 0 | 1.8 | 0 | 0 | 0 | 1.9 | 2.3 | – | 2.3 | 2.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 3.2 |
Endosome | 1.0 | 1.5 | 0.2 | -1.3 | -1.8 | -7.6 | 0 | 0 | -6.2 | -0.5 | 5.1 | 5.5 | – | 2.2 | 1.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 3.9 | 3.6 |
Mitochondria | 1.0 | -1.8 | 2.8 | 2.9 | -0.2 | 9.4 | 0 | 0 | 6.8 | 3.0 | -4.5 | -3.6 | – | -6.3 | -4.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 1.2 | – | 0 | 3.5 |
Nucleolus | 0.3 | -1.7 | -0.9 | -1.6 | -3.3 | -1.6 | 0 | 0 | -2.9 | 4.3 | 3.7 | 3.4 | – | 3.8 | 3.0 |
Peroxisomes | -0.2 | 1.7 | 1.8 | 4.4 | 3.7 | 0.2 | 0 | 0 | 1.5 | -0.4 | -0.6 | 1.5 | – | 0 | 0 |
SpindlePole | -1.7 | 0.7 | -2.2 | 0.4 | 1.8 | -2.9 | 0 | 0 | -0.4 | -4.8 | -6.4 | -7.3 | – | -4.3 | -4.5 |
Vacuole | -2.7 | -4.1 | -5.6 | -8.0 | -7.7 | -7.6 | -8.1 | -6.4 | -5.9 | -0.2 | 0.8 | -3.6 | – | 2.9 | 3.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.5068 | 1.3247 | 1.4958 | 2.0342 | 1.7808 | 1.4231 | -0.5196 | -0.407 | -0.421 | -0.2113 | -0.398 | -0.5169 | -1.2784 | -1.0281 | -1.1938 | -0.9429 | -1.4256 | -1.2656 |
Actin | 0.0311 | 0.0008 | 0.0053 | 0.0017 | 0.0153 | 0.0028 | 0.0304 | 0.0027 | 0.0172 | 0.0025 | 0.0074 | 0.007 | 0.0156 | 0.0012 | 0.0089 | 0.0017 | 0.0668 | 0.0102 |
Bud | 0.0005 | 0.0001 | 0.0003 | 0.0018 | 0.0003 | 0.0004 | 0.0006 | 0.0003 | 0.0003 | 0.0013 | 0.0004 | 0.0002 | 0.0004 | 0.0003 | 0.0016 | 0.0002 | 0.0003 | 0.0002 |
Bud Neck | 0.0033 | 0.0015 | 0.0033 | 0.005 | 0.0075 | 0.0077 | 0.0042 | 0.0006 | 0.001 | 0.0078 | 0.0014 | 0.005 | 0.0024 | 0.0007 | 0.0006 | 0.0011 | 0.0009 | 0.004 |
Bud Periphery | 0.001 | 0.0002 | 0.0014 | 0.0054 | 0.0005 | 0.001 | 0.0014 | 0.0005 | 0.0006 | 0.0038 | 0.0007 | 0.0002 | 0.0006 | 0.0003 | 0.0016 | 0.0001 | 0.0003 | 0.0006 |
Bud Site | 0.0042 | 0.0023 | 0.0039 | 0.0212 | 0.0046 | 0.0015 | 0.0091 | 0.0007 | 0.0019 | 0.0108 | 0.0011 | 0.0005 | 0.006 | 0.0025 | 0.0063 | 0.0022 | 0.0009 | 0.0003 |
Cell Periphery | 0.0005 | 0.0007 | 0.0026 | 0.0019 | 0.0005 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0574 | 0.0699 | 0.0627 | 0.0356 | 0.0537 | 0.0451 | 0.0404 | 0.0976 | 0.051 | 0.0253 | 0.0584 | 0.0624 | 0.0629 | 0.0755 | 0.0556 | 0.0501 | 0.0236 | 0.041 |
Cytoplasmic Foci | 0.0251 | 0.0033 | 0.0108 | 0.0253 | 0.0133 | 0.0128 | 0.0097 | 0.0073 | 0.0108 | 0.0165 | 0.0308 | 0.0065 | 0.0234 | 0.0032 | 0.0049 | 0.01 | 0.0009 | 0.0125 |
Eisosomes | 0.0006 | 0.0003 | 0.0005 | 0.0002 | 0.001 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.014 | 0.0041 | 0.0064 | 0.0019 | 0.0013 | 0.0016 | 0.0059 | 0.0079 | 0.0022 | 0.0014 | 0.0041 | 0.0015 | 0.005 | 0.0047 | 0.004 | 0.0018 | 0.0009 | 0.0017 |
Endosome | 0.0267 | 0.0028 | 0.0088 | 0.0394 | 0.0062 | 0.0044 | 0.0258 | 0.0108 | 0.0199 | 0.0213 | 0.0319 | 0.0043 | 0.0199 | 0.0056 | 0.0071 | 0.0023 | 0.0018 | 0.0054 |
Golgi | 0.0139 | 0.0003 | 0.0025 | 0.0096 | 0.0051 | 0.001 | 0.009 | 0.0006 | 0.0076 | 0.0081 | 0.0218 | 0.0023 | 0.0039 | 0.0004 | 0.0042 | 0.0011 | 0.0019 | 0.006 |
Lipid Particles | 0.0236 | 0.002 | 0.0068 | 0.0176 | 0.011 | 0.0092 | 0.0109 | 0.0024 | 0.0063 | 0.0314 | 0.0523 | 0.0067 | 0.0196 | 0.0014 | 0.0037 | 0.0043 | 0.0002 | 0.0043 |
Mitochondria | 0.0167 | 0.001 | 0.0029 | 0.0193 | 0.0742 | 0.002 | 0.0182 | 0.0028 | 0.0153 | 0.0117 | 0.0311 | 0.0052 | 0.0038 | 0.0011 | 0.0045 | 0.0059 | 0.001 | 0.011 |
None | 0.143 | 0.2754 | 0.2071 | 0.1652 | 0.2966 | 0.1291 | 0.1012 | 0.1268 | 0.1027 | 0.0679 | 0.176 | 0.0626 | 0.083 | 0.1865 | 0.1654 | 0.2373 | 0.0585 | 0.1038 |
Nuclear Periphery | 0.017 | 0.0194 | 0.0193 | 0.0143 | 0.0121 | 0.0136 | 0.0284 | 0.0228 | 0.031 | 0.0514 | 0.0184 | 0.0125 | 0.0364 | 0.0264 | 0.0469 | 0.0363 | 0.026 | 0.0173 |
Nucleolus | 0.0142 | 0.0144 | 0.0156 | 0.0137 | 0.0341 | 0.0193 | 0.0115 | 0.0098 | 0.0116 | 0.0237 | 0.0116 | 0.0174 | 0.0094 | 0.0071 | 0.0065 | 0.0062 | 0.0098 | 0.0125 |
Nucleus | 0.5178 | 0.5557 | 0.5738 | 0.5582 | 0.3235 | 0.668 | 0.6169 | 0.6566 | 0.6366 | 0.6622 | 0.4429 | 0.7068 | 0.6233 | 0.6164 | 0.6404 | 0.5239 | 0.7864 | 0.6913 |
Peroxisomes | 0.0256 | 0.0003 | 0.0035 | 0.0039 | 0.0217 | 0.0052 | 0.0086 | 0.001 | 0.0062 | 0.0083 | 0.0245 | 0.0037 | 0.0152 | 0.0003 | 0.0021 | 0.0304 | 0.0002 | 0.004 |
Punctate Nuclear | 0.0569 | 0.0424 | 0.0587 | 0.0404 | 0.1149 | 0.0728 | 0.0591 | 0.0439 | 0.0748 | 0.0324 | 0.0817 | 0.0939 | 0.0617 | 0.0632 | 0.0333 | 0.084 | 0.0184 | 0.0723 |
Vacuole | 0.0051 | 0.003 | 0.0029 | 0.0153 | 0.002 | 0.0017 | 0.0056 | 0.0037 | 0.0019 | 0.008 | 0.0022 | 0.0009 | 0.0047 | 0.0024 | 0.0013 | 0.0005 | 0.0007 | 0.001 |
Vacuole Periphery | 0.002 | 0.0003 | 0.001 | 0.0031 | 0.0005 | 0.0005 | 0.0021 | 0.0013 | 0.001 | 0.0039 | 0.0011 | 0.0002 | 0.0021 | 0.0006 | 0.0008 | 0.0004 | 0.0003 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.7462 | 10.2876 | 11.9088 | 14.0818 | 11.6383 | 12.882 | 14.5625 | 13.6198 | 13.5592 | 17.2047 |
Translational Efficiency | 0.9551 | 0.6615 | 0.5996 | 0.4794 | 0.5246 | 0.5059 | 0.5581 | 0.4164 | 0.4481 | 0.3899 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1969 | 539 | 560 | 1014 | 1127 | 793 | 221 | 2638 | 3096 | 1332 | 781 | 3652 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 651.87 | 725.48 | 933.00 | 863.66 | 685.09 | 749.21 | 922.08 | 934.87 | 663.96 | 739.61 | 929.91 | 915.10 |
Standard Deviation | 64.71 | 102.01 | 147.32 | 145.26 | 80.25 | 112.48 | 91.04 | 135.43 | 72.55 | 108.99 | 133.91 | 141.86 |
Intensity Change Log 2 | — | 0.154352 | 0.517293 | 0.405879 | — | 0.129077 | 0.428598 | 0.448472 | — | 0.141455 | 0.472525 | 0.427862 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000040 | 0.000367 | 0.001186 | 0.002667 | 0.000195 | 0.000527 | 0.000540 | 0.000299 | 0.000097 | 0.000462 | 0.001003 | 0.000957 |
Bud Neck | 0.005204 | 0.007670 | 0.008422 | 0.021437 | 0.002630 | 0.014046 | 0.002532 | 0.036954 | 0.004267 | 0.011466 | 0.006755 | 0.032646 |
Bud Site | 0.001150 | 0.002298 | 0.003502 | 0.043195 | 0.000956 | 0.007739 | 0.000884 | 0.012111 | 0.001079 | 0.005537 | 0.002761 | 0.020742 |
Cell Periphery | 0.000095 | 0.000157 | 0.000102 | 0.001663 | 0.000123 | 0.000219 | 0.000095 | 0.000141 | 0.000105 | 0.000194 | 0.000100 | 0.000563 |
Cytoplasm | 0.305754* | 0.200924* | 0.013112 | 0.077945 | 0.299764* | 0.181666* | 0.031101 | 0.128927* | 0.303574* | 0.189459* | 0.018202 | 0.114772* |
Cytoplasmic Foci | 0.055774 | 0.074626 | 0.000968 | 0.026576 | 0.062067 | 0.083859 | 0.003314 | 0.000412 | 0.058065 | 0.080123 | 0.001632 | 0.007677 |
Eisosomes | 0.000045 | 0.000082 | 0.000036 | 0.000072 | 0.000052 | 0.000089 | 0.000061 | 0.000015 | 0.000047 | 0.000086 | 0.000043 | 0.000031 |
Endoplasmic Reticulum | 0.001219 | 0.002784 | 0.010108 | 0.009387 | 0.001732 | 0.002509 | 0.007664 | 0.002498 | 0.001406 | 0.002620 | 0.009416 | 0.004411 |
Endosome | 0.001305 | 0.005190 | 0.000781 | 0.027800 | 0.002523 | 0.006316 | 0.001078 | 0.000705 | 0.001748 | 0.005861 | 0.000865 | 0.008228 |
Golgi | 0.000371 | 0.002733 | 0.005659 | 0.021070 | 0.001804 | 0.004020 | 0.000062 | 0.000078 | 0.000893 | 0.003499 | 0.004075 | 0.005907 |
Lipid Particles | 0.008917 | 0.012925 | 0.000417 | 0.002435 | 0.008087 | 0.016043 | 0.001419 | 0.000253 | 0.008615 | 0.014781 | 0.000701 | 0.000859 |
Mitochondria | 0.005626 | 0.012885 | 0.001370 | 0.017380 | 0.002921 | 0.021071 | 0.000515 | 0.007384 | 0.004641 | 0.017759 | 0.001128 | 0.010160 |
Mitotic Spindle | 0.002082 | 0.010327 | 0.001892 | 0.079655 | 0.003599 | 0.025265 | 0.000177 | 0.022089 | 0.002634 | 0.019220 | 0.001407 | 0.038073 |
None | 0.009558 | 0.012441 | 0.004272 | 0.007959 | 0.014264 | 0.010212 | 0.004315 | 0.008728 | 0.011271 | 0.011114 | 0.004284 | 0.008514 |
Nuclear Periphery | 0.003321 | 0.003061 | 0.001099 | 0.006249 | 0.002082 | 0.003921 | 0.001529 | 0.003632 | 0.002870 | 0.003573 | 0.001221 | 0.004358 |
Nuclear Periphery Foci | 0.000876 | 0.002350 | 0.000496 | 0.002566 | 0.001501 | 0.002790 | 0.000697 | 0.000132 | 0.001104 | 0.002612 | 0.000553 | 0.000807 |
Nucleolus | 0.005572 | 0.009978 | 0.003640 | 0.004597 | 0.004910 | 0.011770 | 0.002112 | 0.001603 | 0.005331 | 0.011045 | 0.003208 | 0.002434 |
Nucleus | 0.585120* | 0.616589* | 0.935222* | 0.556927* | 0.581499* | 0.582124* | 0.925721* | 0.761704* | 0.583802* | 0.596070* | 0.932533* | 0.704846* |
Peroxisomes | 0.002786 | 0.009489 | 0.000225 | 0.003231 | 0.003142 | 0.009553 | 0.000255 | 0.000114 | 0.002916 | 0.009527 | 0.000234 | 0.000980 |
Vacuole | 0.004861 | 0.011910 | 0.006895 | 0.063009 | 0.005729 | 0.014698 | 0.015809 | 0.011368 | 0.005177 | 0.013570 | 0.009417 | 0.025706 |
Vacuole Periphery | 0.000323 | 0.001215 | 0.000596 | 0.024181 | 0.000419 | 0.001564 | 0.000123 | 0.000853 | 0.000358 | 0.001423 | 0.000462 | 0.007330 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.83 | -10.82 | -6.31 | -5.10 | -1.16 | -3.33 | -5.58 | -2.17 | 2.16 | 4.84 | -6.47 | -11.78 | -7.62 | -3.15 | 4.25 |
Bud Neck | -1.72 | -6.76 | -8.51 | -5.98 | -3.40 | -6.85 | -3.53 | -18.59 | -8.39 | -17.13 | -6.15 | -7.60 | -19.32 | -10.88 | -12.69 |
Bud Site | -2.22 | -2.66 | -8.01 | -7.57 | -6.60 | -4.51 | -3.11 | -8.57 | -0.69 | -7.52 | -4.80 | -3.01 | -11.33 | -5.69 | -7.42 |
Cell Periphery | -4.48 | -1.60 | -1.22 | -1.10 | -1.19 | -4.10 | -0.11 | 2.81 | 5.23 | 1.87 | -6.13 | -1.12 | -1.18 | -0.54 | -1.12 |
Cytoplasm | 9.46 | 52.84 | 34.52 | 12.09 | -14.15 | 10.87 | 29.78 | 24.44 | 10.18 | -12.32 | 15.27 | 60.43 | 42.15 | 15.10 | -25.22 |
Cytoplasmic Foci | -3.39 | 22.19 | 15.51 | 11.51 | -6.35 | -3.53 | 16.19 | 17.60 | 17.72 | 2.41 | -5.49 | 27.62 | 26.46 | 21.53 | -3.50 |
Eisosomes | -4.75 | 0.44 | -3.93 | 0.93 | -4.02 | -5.21 | -3.39 | 12.61 | 11.18 | 7.53 | -7.60 | -2.05 | 5.18 | 9.79 | 5.48 |
Endoplasmic Reticulum | -4.55 | -15.92 | -20.62 | -16.50 | -2.51 | -2.64 | -8.79 | -7.50 | -4.38 | 7.15 | -5.74 | -18.10 | -21.34 | -14.24 | 8.70 |
Endosome | -4.87 | 2.67 | -7.65 | -3.95 | -8.00 | -4.64 | 3.01 | 7.45 | 7.68 | 4.91 | -7.25 | 4.38 | -4.47 | 3.52 | -6.40 |
Golgi | -2.33 | -1.27 | -4.92 | -2.86 | -1.98 | -1.67 | 2.72 | 2.73 | 3.58 | 0.59 | -3.17 | -0.83 | -2.69 | 1.37 | -0.07 |
Lipid Particles | -2.72 | 9.83 | 7.90 | 8.13 | -6.87 | -4.16 | 7.69 | 10.23 | 9.51 | 5.23 | -4.84 | 12.60 | 12.91 | 12.48 | 1.15 |
Mitochondria | -3.25 | 4.90 | -1.52 | 1.69 | -4.25 | -6.63 | 3.93 | -3.19 | 5.60 | -7.95 | -6.92 | 5.84 | -2.00 | 5.93 | -7.89 |
Mitotic Spindle | -2.43 | -0.58 | -9.02 | -6.18 | -8.82 | -4.74 | 2.24 | -7.15 | 0.83 | -10.06 | -5.49 | 0.45 | -11.97 | -2.60 | -12.21 |
None | -1.49 | 8.40 | 3.42 | 3.51 | -2.64 | 2.09 | 7.26 | 4.43 | 1.84 | -6.20 | 0.25 | 10.96 | 5.48 | 3.59 | -7.62 |
Nuclear Periphery | 0.52 | 3.70 | -4.28 | -4.59 | -7.11 | -2.23 | -0.28 | -9.60 | -2.77 | -13.31 | -1.14 | 3.34 | -8.43 | -5.08 | -15.83 |
Nuclear Periphery Foci | -2.13 | 0.09 | -4.01 | -0.11 | -3.37 | -1.10 | 2.25 | 6.63 | 2.56 | 4.55 | -2.16 | 1.01 | 2.15 | 2.68 | 0.35 |
Nucleolus | -2.79 | 3.64 | 4.00 | 4.98 | 0.63 | -4.52 | 4.86 | 5.20 | 7.04 | -0.18 | -5.05 | 5.04 | 7.65 | 8.85 | 2.17 |
Nucleus | -1.74 | -43.50 | -7.32 | -3.66 | 21.90 | 0.13 | -25.35 | -21.24 | -17.48 | 10.57 | -0.77 | -50.20 | -26.23 | -17.25 | 25.10 |
Peroxisomes | -4.84 | 5.69 | 1.61 | 5.55 | -2.83 | -5.39 | 5.36 | 5.79 | 8.54 | 4.65 | -7.33 | 7.71 | 6.40 | 10.50 | -1.85 |
Vacuole | -4.55 | -4.41 | -16.55 | -13.56 | -14.68 | -4.98 | -5.10 | -8.89 | 0.07 | 3.07 | -6.93 | -6.52 | -18.39 | -9.07 | -7.47 |
Vacuole Periphery | -4.05 | -0.53 | -5.38 | -4.82 | -5.31 | -4.44 | 2.42 | -0.09 | 4.25 | -2.23 | -6.21 | 0.15 | -5.30 | -2.98 | -5.22 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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