Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the HOPS and the CORVET complexes; part of the Class C Vps complex essential for membrane docking and fusion at Golgi-to-endosome and endosome-to-vacuole protein transport stages |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 5 | 2 | 2 | 1 | 2 | 1 | 0 | 1 | – | 0 | 1 | 0 | 3 | 2 | 0 | 1 | 3 |
Bud | 1 | 2 | 11 | 6 | 2 | 13 | 28 | 38 | 16 | 28 | 13 | 27 | 3 | 2 | 2 | – | 1 | 2 | 0 | 1 | 0 | 1 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 3 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 1 | 0 | 1 | 0 | 1 | 1 | 6 | 2 | 3 | 4 | 4 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 9 | 2 | 6 | 12 | 5 | 22 | 23 | 8 | 10 | 9 | 5 | 5 | 11 | 14 | – | 15 | 12 | 2 | 2 | 3 | 1 | 0 | 0 |
Endoplasmic Reticulum | 4 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 4 | 3 | 2 | 2 | 2 | 4 | – | 12 | 16 | 0 | 1 | 1 | 1 | 0 | 0 |
Endosome | 79 | 70 | 120 | 112 | 162 | 175 | 199 | 176 | 42 | 34 | 22 | 59 | 91 | 253 | 266 | – | 232 | 176 | 136 | 180 | 207 | 137 | 261 | 305 |
Golgi | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 28 | 19 | 6 | 4 | 6 | 0 | 2 | 9 |
Mitochondria | 88 | 114 | 72 | 70 | 65 | 143 | 186 | 121 | 182 | 244 | 151 | 157 | 85 | 52 | 64 | – | 40 | 41 | 11 | 13 | 11 | 3 | 5 | 4 |
Nucleus | 2 | 2 | 5 | 2 | 0 | 4 | 5 | 2 | 3 | 0 | 3 | 0 | 1 | 1 | 0 | – | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 12 | 8 | 3 | 0 | 0 | 1 | 2 | 1 | 2 | 3 | 1 | 1 | 6 | 17 | 10 | – | 9 | 24 | 17 | 5 | 11 | 6 | 16 | 11 |
Nucleolus | 54 | 42 | 24 | 22 | 17 | 27 | 29 | 15 | 15 | 24 | 12 | 9 | 49 | 77 | 75 | – | 86 | 59 | 0 | 2 | 1 | 1 | 0 | 2 |
Peroxisomes | 3 | 1 | 8 | 6 | 19 | 23 | 63 | 49 | 9 | 15 | 7 | 16 | 5 | 9 | 22 | – | 1 | 1 | 1 | 1 | 5 | 0 | 2 | 6 |
SpindlePole | 27 | 11 | 81 | 53 | 104 | 87 | 157 | 170 | 54 | 86 | 30 | 82 | 26 | 42 | 35 | – | 73 | 52 | 7 | 5 | 6 | 7 | 11 | 3 |
Vac/Vac Membrane | 43 | 74 | 72 | 38 | 43 | 39 | 31 | 30 | 10 | 16 | 10 | 23 | 56 | 118 | 64 | – | 164 | 140 | 19 | 33 | 29 | 13 | 23 | 22 |
Unique Cell Count | 198 | 233 | 254 | 215 | 301 | 365 | 473 | 437 | 261 | 345 | 200 | 271 | 203 | 375 | 386 | 415 | 326 | 205 | 260 | 291 | 179 | 331 | 374 | |
Labelled Cell Count | 320 | 334 | 402 | 315 | 429 | 521 | 732 | 636 | 348 | 472 | 267 | 384 | 331 | 585 | 557 | 663 | 545 | 205 | 260 | 291 | 179 | 331 | 374 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.5 | 6.0 | 6.3 | 5.7 | 6.6 | 5.2 | 5.4 | 5.7 | 4.9 | 4.6 | 4.5 | 5.1 | 6.4 | 6.8 | 6.7 | – | 10.3 | 9.4 | 5.9 | 6.1 | 6.7 |
Std Deviation (1e-4) | 1.0 | 1.3 | 0.9 | 0.7 | 1.3 | 0.6 | 1.2 | 1.0 | 1.0 | 0.9 | 0.8 | 1.0 | 1.1 | 0.9 | 1.1 | – | 2.1 | 1.7 | 1.1 | 0.9 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.15 | 0.07 | -0.27 | -0.22 | -0.13 | -0.37 | -0.46 | -0.47 | -0.29 | 0.02 | 0.1 | 0.1 | – | 0.71 | 0.58 | -0.09 | -0.06 | 0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud | -0.9 | -2.8 | -0.5 | 0.9 | 2.2 | 0.9 | 0 | 0 | 2.5 | -1.8 | -3.3 | -3.3 | – | 0 | -3.0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cytoplasm | 0 | 2.4 | 0 | 2.8 | 3.0 | 0 | 1.8 | 0 | 0 | 0 | 1.9 | 2.3 | – | 2.3 | 2.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 3.2 |
Endosome | 1.0 | 1.5 | 0.2 | -1.3 | -1.8 | -7.6 | 0 | 0 | -6.2 | -0.5 | 5.1 | 5.5 | – | 2.2 | 1.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 3.9 | 3.6 |
Mitochondria | 1.0 | -1.8 | 2.8 | 2.9 | -0.2 | 9.4 | 0 | 0 | 6.8 | 3.0 | -4.5 | -3.6 | – | -6.3 | -4.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 1.2 | – | 0 | 3.5 |
Nucleolus | 0.3 | -1.7 | -0.9 | -1.6 | -3.3 | -1.6 | 0 | 0 | -2.9 | 4.3 | 3.7 | 3.4 | – | 3.8 | 3.0 |
Peroxisomes | -0.2 | 1.7 | 1.8 | 4.4 | 3.7 | 0.2 | 0 | 0 | 1.5 | -0.4 | -0.6 | 1.5 | – | 0 | 0 |
SpindlePole | -1.7 | 0.7 | -2.2 | 0.4 | 1.8 | -2.9 | 0 | 0 | -0.4 | -4.8 | -6.4 | -7.3 | – | -4.3 | -4.5 |
Vacuole | -2.7 | -4.1 | -5.6 | -8.0 | -7.7 | -7.6 | -8.1 | -6.4 | -5.9 | -0.2 | 0.8 | -3.6 | – | 2.9 | 3.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.5068 | 1.3247 | 1.4958 | 2.0342 | 1.7808 | 1.4231 | -0.5196 | -0.407 | -0.421 | -0.2113 | -0.398 | -0.5169 | -1.2784 | -1.0281 | -1.1938 | -0.9429 | -1.4256 | -1.2656 |
Actin | 0.0311 | 0.0008 | 0.0053 | 0.0017 | 0.0153 | 0.0028 | 0.0304 | 0.0027 | 0.0172 | 0.0025 | 0.0074 | 0.007 | 0.0156 | 0.0012 | 0.0089 | 0.0017 | 0.0668 | 0.0102 |
Bud | 0.0005 | 0.0001 | 0.0003 | 0.0018 | 0.0003 | 0.0004 | 0.0006 | 0.0003 | 0.0003 | 0.0013 | 0.0004 | 0.0002 | 0.0004 | 0.0003 | 0.0016 | 0.0002 | 0.0003 | 0.0002 |
Bud Neck | 0.0033 | 0.0015 | 0.0033 | 0.005 | 0.0075 | 0.0077 | 0.0042 | 0.0006 | 0.001 | 0.0078 | 0.0014 | 0.005 | 0.0024 | 0.0007 | 0.0006 | 0.0011 | 0.0009 | 0.004 |
Bud Periphery | 0.001 | 0.0002 | 0.0014 | 0.0054 | 0.0005 | 0.001 | 0.0014 | 0.0005 | 0.0006 | 0.0038 | 0.0007 | 0.0002 | 0.0006 | 0.0003 | 0.0016 | 0.0001 | 0.0003 | 0.0006 |
Bud Site | 0.0042 | 0.0023 | 0.0039 | 0.0212 | 0.0046 | 0.0015 | 0.0091 | 0.0007 | 0.0019 | 0.0108 | 0.0011 | 0.0005 | 0.006 | 0.0025 | 0.0063 | 0.0022 | 0.0009 | 0.0003 |
Cell Periphery | 0.0005 | 0.0007 | 0.0026 | 0.0019 | 0.0005 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0574 | 0.0699 | 0.0627 | 0.0356 | 0.0537 | 0.0451 | 0.0404 | 0.0976 | 0.051 | 0.0253 | 0.0584 | 0.0624 | 0.0629 | 0.0755 | 0.0556 | 0.0501 | 0.0236 | 0.041 |
Cytoplasmic Foci | 0.0251 | 0.0033 | 0.0108 | 0.0253 | 0.0133 | 0.0128 | 0.0097 | 0.0073 | 0.0108 | 0.0165 | 0.0308 | 0.0065 | 0.0234 | 0.0032 | 0.0049 | 0.01 | 0.0009 | 0.0125 |
Eisosomes | 0.0006 | 0.0003 | 0.0005 | 0.0002 | 0.001 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.014 | 0.0041 | 0.0064 | 0.0019 | 0.0013 | 0.0016 | 0.0059 | 0.0079 | 0.0022 | 0.0014 | 0.0041 | 0.0015 | 0.005 | 0.0047 | 0.004 | 0.0018 | 0.0009 | 0.0017 |
Endosome | 0.0267 | 0.0028 | 0.0088 | 0.0394 | 0.0062 | 0.0044 | 0.0258 | 0.0108 | 0.0199 | 0.0213 | 0.0319 | 0.0043 | 0.0199 | 0.0056 | 0.0071 | 0.0023 | 0.0018 | 0.0054 |
Golgi | 0.0139 | 0.0003 | 0.0025 | 0.0096 | 0.0051 | 0.001 | 0.009 | 0.0006 | 0.0076 | 0.0081 | 0.0218 | 0.0023 | 0.0039 | 0.0004 | 0.0042 | 0.0011 | 0.0019 | 0.006 |
Lipid Particles | 0.0236 | 0.002 | 0.0068 | 0.0176 | 0.011 | 0.0092 | 0.0109 | 0.0024 | 0.0063 | 0.0314 | 0.0523 | 0.0067 | 0.0196 | 0.0014 | 0.0037 | 0.0043 | 0.0002 | 0.0043 |
Mitochondria | 0.0167 | 0.001 | 0.0029 | 0.0193 | 0.0742 | 0.002 | 0.0182 | 0.0028 | 0.0153 | 0.0117 | 0.0311 | 0.0052 | 0.0038 | 0.0011 | 0.0045 | 0.0059 | 0.001 | 0.011 |
None | 0.143 | 0.2754 | 0.2071 | 0.1652 | 0.2966 | 0.1291 | 0.1012 | 0.1268 | 0.1027 | 0.0679 | 0.176 | 0.0626 | 0.083 | 0.1865 | 0.1654 | 0.2373 | 0.0585 | 0.1038 |
Nuclear Periphery | 0.017 | 0.0194 | 0.0193 | 0.0143 | 0.0121 | 0.0136 | 0.0284 | 0.0228 | 0.031 | 0.0514 | 0.0184 | 0.0125 | 0.0364 | 0.0264 | 0.0469 | 0.0363 | 0.026 | 0.0173 |
Nucleolus | 0.0142 | 0.0144 | 0.0156 | 0.0137 | 0.0341 | 0.0193 | 0.0115 | 0.0098 | 0.0116 | 0.0237 | 0.0116 | 0.0174 | 0.0094 | 0.0071 | 0.0065 | 0.0062 | 0.0098 | 0.0125 |
Nucleus | 0.5178 | 0.5557 | 0.5738 | 0.5582 | 0.3235 | 0.668 | 0.6169 | 0.6566 | 0.6366 | 0.6622 | 0.4429 | 0.7068 | 0.6233 | 0.6164 | 0.6404 | 0.5239 | 0.7864 | 0.6913 |
Peroxisomes | 0.0256 | 0.0003 | 0.0035 | 0.0039 | 0.0217 | 0.0052 | 0.0086 | 0.001 | 0.0062 | 0.0083 | 0.0245 | 0.0037 | 0.0152 | 0.0003 | 0.0021 | 0.0304 | 0.0002 | 0.004 |
Punctate Nuclear | 0.0569 | 0.0424 | 0.0587 | 0.0404 | 0.1149 | 0.0728 | 0.0591 | 0.0439 | 0.0748 | 0.0324 | 0.0817 | 0.0939 | 0.0617 | 0.0632 | 0.0333 | 0.084 | 0.0184 | 0.0723 |
Vacuole | 0.0051 | 0.003 | 0.0029 | 0.0153 | 0.002 | 0.0017 | 0.0056 | 0.0037 | 0.0019 | 0.008 | 0.0022 | 0.0009 | 0.0047 | 0.0024 | 0.0013 | 0.0005 | 0.0007 | 0.001 |
Vacuole Periphery | 0.002 | 0.0003 | 0.001 | 0.0031 | 0.0005 | 0.0005 | 0.0021 | 0.0013 | 0.001 | 0.0039 | 0.0011 | 0.0002 | 0.0021 | 0.0006 | 0.0008 | 0.0004 | 0.0003 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.7462 | 10.2876 | 11.9088 | 14.0818 | 11.6383 | 12.882 | 14.5625 | 13.6198 | 13.5592 | 17.2047 |
Translational Efficiency | 0.9551 | 0.6615 | 0.5996 | 0.4794 | 0.5246 | 0.5059 | 0.5581 | 0.4164 | 0.4481 | 0.3899 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|