Standard name | |
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Human Ortholog | |
Description | Integral subunit of SAGA histone acetyltransferase complex; regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1 | 0 | 1 | – | 2 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Bud Site | 0 | 0 | 1 | 1 | 0 | 6 | – | 8 | 6 | 4 | 7 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 6 | 6 | 10 | 12 | 4 | – | 8 | 7 | 8 | 5 | 4 | 17 | 47 | 67 | 14 | 9 | 18 | 0 | 0 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Endosome | 0 | 2 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 2 | 1 | 2 | 1 | 4 | 1 | 0 | 4 | 7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 4 | 0 | 1 | 1 | 0 | 1 | 0 |
Mitochondria | 14 | 7 | 60 | 35 | 13 | 66 | – | 140 | 131 | 109 | 67 | 96 | 4 | 0 | 1 | 3 | 4 | 9 | 1 | 2 | 2 | 1 | 0 | 4 |
Nucleus | 215 | 389 | 291 | 161 | 209 | 198 | – | 313 | 211 | 173 | 140 | 120 | 237 | 224 | 259 | 102 | 94 | 108 | 224 | 384 | 279 | 147 | 163 | 172 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 0 | 1 | – | 3 | 1 | 2 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 29 | 40 | 41 | 29 | 28 | 64 | – | 98 | 73 | 63 | 51 | 39 | 35 | 23 | 27 | 4 | 10 | 13 | 8 | 19 | 21 | 39 | 49 | 50 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 0 | – | 1 | 0 | 1 | 2 | 0 | 1 | 2 | 4 | 0 | 0 | 1 | 1 | 1 | 2 | 3 | 1 | 6 |
Vac/Vac Membrane | 0 | 5 | 1 | 0 | 2 | 4 | – | 6 | 9 | 3 | 2 | 1 | 0 | 2 | 1 | 3 | 5 | 7 | 6 | 8 | 6 | 9 | 10 | 31 |
Unique Cell Count | 237 | 418 | 314 | 187 | 246 | 257 | 429 | 294 | 252 | 188 | 177 | 269 | 282 | 338 | 129 | 120 | 152 | 247 | 431 | 324 | 207 | 236 | 288 | |
Labelled Cell Count | 260 | 451 | 402 | 238 | 265 | 344 | 579 | 439 | 366 | 278 | 264 | 299 | 303 | 368 | 133 | 127 | 163 | 247 | 431 | 324 | 207 | 236 | 288 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.8 | 9.8 | 6.0 | 7.2 | 7.7 | 5.8 | – | 5.5 | 5.0 | 5.2 | 5.2 | 4.8 | 9.7 | 9.4 | 8.8 | 13.5 | 13.2 | 12.1 | 9.6 | 10.1 | 9.6 |
Std Deviation (1e-4) | 1.3 | 1.7 | 1.5 | 2.0 | 1.9 | 1.2 | – | 1.3 | 1.5 | 1.6 | 1.5 | 0.9 | 2.6 | 2.8 | 2.6 | 3.7 | 3.0 | 3.2 | 2.1 | 1.9 | 2.4 |
Intensity Change (Log2) | – | – | – | 0.26 | 0.36 | -0.05 | – | -0.12 | -0.26 | -0.22 | -0.21 | -0.34 | 0.69 | 0.64 | 0.55 | 1.16 | 1.12 | 1.0 | 0.67 | 0.75 | 0.67 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.1 | 2.0 | 0 | – | 0 | 0.4 | 1.0 | 0 | 0 | 2.7 | 6.3 | 7.2 | 4.1 | 0 | 4.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.1 | -4.8 | 1.9 | – | 4.1 | 6.8 | 6.2 | 4.1 | 0 | -6.8 | -7.7 | -8.2 | -4.6 | -4.1 | -3.8 |
Nucleus | -2.4 | -2.9 | -5.3 | – | -6.8 | -6.8 | -7.4 | -5.7 | -7.2 | -1.9 | -4.7 | -5.6 | -4.1 | -4.2 | -6.2 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.8 | -0.6 | 3.6 | – | 3.4 | 3.7 | 3.6 | 3.9 | 0 | 0 | -1.9 | -2.1 | -3.2 | -1.4 | -1.4 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.3207 | 5.2079 | 5.0714 | 5.0925 | 4.7804 | 5.1879 | 3.5095 | 3.7287 | 3.617 | 3.6466 | 3.4853 | 3.7623 | 7.6011 | 7.328 | 6.986 | 6.4737 | 6.6337 | 6.9885 |
Actin | 0.0029 | 0 | 0.0077 | 0 | 0 | 0 | 0.0274 | 0.0001 | 0.0004 | 0.0037 | 0.0047 | 0.0019 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0008 | 0.0011 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 |
Bud Neck | 0.0005 | 0.0006 | 0.0006 | 0.0001 | 0.0011 | 0.0006 | 0.0058 | 0.0002 | 0.0004 | 0.0012 | 0.003 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0004 |
Bud Periphery | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0019 | 0.0003 | 0.0005 | 0.0014 | 0.0015 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 |
Bud Site | 0.002 | 0.0008 | 0.001 | 0 | 0.0003 | 0.0001 | 0.0059 | 0.0019 | 0.0022 | 0.0016 | 0.0036 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0007 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0063 | 0.0048 | 0.0005 | 0.001 | 0.1097 | 0.0003 | 0.0075 | 0.0014 | 0.0057 | 0.0056 | 0.0132 | 0.0001 | 0.0002 | 0.003 | 0.0001 | 0 | 0 | 0.0001 |
Cytoplasmic Foci | 0.0047 | 0.0004 | 0.0005 | 0.0103 | 0.0114 | 0.0006 | 0.0103 | 0.0012 | 0.0042 | 0.0012 | 0.0069 | 0.0007 | 0 | 0.0003 | 0 | 0 | 0 | 0 |
Eisosomes | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0012 | 0.0002 | 0.0007 | 0 | 0.0002 | 0 | 0.0046 | 0.0002 | 0.0012 | 0.0036 | 0.0073 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 |
Endosome | 0.0066 | 0.0009 | 0.0013 | 0 | 0.0112 | 0 | 0.0178 | 0.0014 | 0.0133 | 0.0096 | 0.0461 | 0.0035 | 0 | 0.0009 | 0 | 0 | 0 | 0.0003 |
Golgi | 0.0013 | 0 | 0.0005 | 0 | 0.0002 | 0 | 0.004 | 0.0002 | 0.002 | 0.0013 | 0.0147 | 0.0044 | 0 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0.0019 | 0.0001 | 0.0016 | 0.0001 | 0 | 0 | 0.0045 | 0.0011 | 0.0041 | 0.0017 | 0.0348 | 0.0031 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Mitochondria | 0.0008 | 0.0003 | 0.0007 | 0.0001 | 0.0004 | 0.0001 | 0.0069 | 0.0005 | 0.0051 | 0.0115 | 0.0054 | 0.0029 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 |
None | 0.0019 | 0.0075 | 0.0007 | 0.0013 | 0.0005 | 0.0001 | 0.003 | 0.0005 | 0.0072 | 0.0011 | 0.001 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0097 | 0.0051 | 0.0046 | 0.0006 | 0.0012 | 0.0004 | 0.0227 | 0.0024 | 0.0017 | 0.0147 | 0.0228 | 0.0006 | 0.0005 | 0.0029 | 0.0004 | 0.0001 | 0.0002 | 0.0019 |
Nucleolus | 0.0267 | 0.0214 | 0.0144 | 0.012 | 0.0596 | 0.0432 | 0.0143 | 0.0134 | 0.0148 | 0.0098 | 0.0536 | 0.0382 | 0.0077 | 0.0123 | 0.0057 | 0.0069 | 0.0333 | 0.0196 |
Nucleus | 0.9046 | 0.9314 | 0.9601 | 0.8879 | 0.7947 | 0.9462 | 0.8426 | 0.9727 | 0.9299 | 0.9071 | 0.7543 | 0.9417 | 0.9906 | 0.9675 | 0.9931 | 0.9929 | 0.9659 | 0.976 |
Peroxisomes | 0.0009 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0023 | 0.0002 | 0.0005 | 0.0004 | 0.0009 | 0.0007 | 0 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0267 | 0.0232 | 0.0035 | 0.0865 | 0.0015 | 0.0081 | 0.0063 | 0.0006 | 0.0028 | 0.0181 | 0.0143 | 0.0008 | 0.0005 | 0.0086 | 0.0002 | 0.0001 | 0.0004 | 0.0005 |
Vacuole | 0.0006 | 0.0024 | 0.0003 | 0 | 0.0065 | 0.0001 | 0.008 | 0.0013 | 0.0028 | 0.0032 | 0.0073 | 0.0002 | 0.0001 | 0.0032 | 0.0001 | 0 | 0 | 0.0003 |
Vacuole Periphery | 0.0003 | 0.0004 | 0.0003 | 0 | 0.001 | 0 | 0.0023 | 0.0003 | 0.0007 | 0.0019 | 0.0029 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.7121 | 23.0154 | 17.0785 | 17.0175 | 26.2279 | 11.0279 | 19.1154 | 20.1938 | 18.7395 | 19.9452 |
Translational Efficiency | 1.3575 | 1.0119 | 0.9399 | 0.7584 | 0.6053 | 2.3691 | 1.0773 | 1.0726 | 1.3669 | 1.2178 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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