Standard name | |
---|---|
Human Ortholog | |
Description | Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 3 | – | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 2 | 2 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 10 | 17 | 18 | 46 | 85 | 106 | 159 | 175 | – | 185 | 218 | 246 | 39 | 99 | 166 | 15 | 7 | 20 | 1 | 1 | 1 | 2 | 1 | 1 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 2 | 1 | 10 | 4 | – | 2 | 0 | 3 | 0 | 3 | 0 | 15 | 9 | 6 | 0 | 0 | 1 | 1 | 1 | 2 |
Endosome | 14 | 8 | 5 | 2 | 21 | 2 | 5 | 8 | – | 4 | 2 | 5 | 23 | 56 | 65 | 6 | 6 | 7 | 17 | 7 | 11 | 12 | 18 | 24 |
Golgi | 8 | 2 | 4 | 0 | 3 | 0 | 1 | 0 | – | 0 | 0 | 0 | 12 | 8 | 2 | 0 | 2 | 2 | 9 | 1 | 4 | 4 | 4 | 5 |
Mitochondria | 50 | 10 | 40 | 12 | 61 | 55 | 67 | 58 | – | 57 | 100 | 102 | 4 | 3 | 2 | 5 | 6 | 2 | 6 | 0 | 4 | 5 | 7 | 5 |
Nucleus | 82 | 32 | 64 | 93 | 59 | 188 | 233 | 232 | – | 130 | 129 | 136 | 28 | 24 | 16 | 166 | 109 | 83 | 18 | 4 | 5 | 5 | 6 | 4 |
Nuclear Periphery | 6 | 0 | 4 | 3 | 6 | 4 | 10 | 3 | – | 5 | 3 | 15 | 0 | 1 | 0 | 0 | 1 | 1 | 7 | 1 | 2 | 1 | 3 | 4 |
Nucleolus | 20 | 1 | 2 | 2 | 5 | 1 | 10 | 3 | – | 6 | 1 | 8 | 1 | 1 | 2 | 2 | 1 | 3 | 9 | 0 | 0 | 0 | 2 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 2 | 2 | 1 | 0 | 2 | 2 | 0 |
Vac/Vac Membrane | 278 | 219 | 247 | 292 | 590 | 566 | 715 | 932 | – | 750 | 519 | 768 | 295 | 321 | 296 | 173 | 161 | 157 | 404 | 240 | 298 | 255 | 323 | 358 |
Unique Cell Count | 400 | 254 | 321 | 381 | 708 | 789 | 1023 | 1214 | 989 | 755 | 1071 | 343 | 426 | 452 | 315 | 253 | 240 | 484 | 266 | 336 | 298 | 376 | 418 | |
Labelled Cell Count | 468 | 290 | 384 | 450 | 834 | 924 | 1210 | 1419 | 1140 | 975 | 1287 | 402 | 517 | 557 | 383 | 303 | 283 | 484 | 266 | 336 | 298 | 376 | 418 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.1 | 11.6 | 8.9 | 9.3 | 10.3 | 9.9 | 9.5 | 9.7 | – | 9.6 | 8.6 | 9.3 | 9.6 | 8.2 | 7.4 | 13.8 | 13.2 | 14.0 | 11.9 | 13.1 | 13.2 |
Std Deviation (1e-4) | 4.5 | 4.6 | 3.4 | 3.3 | 3.6 | 4.0 | 3.8 | 3.6 | – | 3.6 | 3.5 | 3.3 | 3.4 | 2.6 | 2.1 | 5.3 | 5.9 | 5.5 | 4.1 | 4.4 | 4.2 |
Intensity Change (Log2) | – | – | – | 0.07 | 0.21 | 0.16 | 0.09 | 0.12 | – | 0.12 | -0.05 | 0.06 | 0.12 | -0.12 | -0.26 | 0.64 | 0.57 | 0.66 | 0.42 | 0.56 | 0.58 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.0 | 3.2 | 3.8 | 4.6 | 4.2 | – | 5.6 | 8.4 | 7.0 | 2.6 | 6.6 | 10.0 | -0.5 | -1.7 | 1.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 4.0 | 0 | 0 |
Endosome | 0 | 1.3 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.3 | 5.7 | 6.1 | 0.3 | 0 | 1.1 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1.9 | 0.7 | 0 | 0 | 0 | 0 |
Mitochondria | -4.7 | -1.9 | -3.0 | -3.4 | -5.0 | – | -4.0 | 0.3 | -1.5 | -5.8 | -6.8 | -7.3 | -5.3 | -4.4 | -5.2 |
Nucleus | 1.4 | -5.3 | 1.4 | 1.1 | -0.3 | – | -3.0 | -1.1 | -3.2 | -4.4 | -6.0 | -7.4 | 8.6 | 6.0 | 3.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.1 | 2.4 | -1.8 | -2.4 | -0.1 | – | -0.4 | -2.7 | -1.9 | 3.0 | -0.5 | -3.4 | -5.9 | -3.5 | -3.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.8707 | 5.0718 | 4.6876 | 3.8873 | 3.3858 | 4.4756 | 5.3353 | 10.0148 | 7.8611 | 7.7512 | 7.4862 | 8.462 | 2.8887 | 5.8233 | 5.266 | 5.251 | 4.7443 | 5.1881 |
Actin | 0.0141 | 0 | 0.0006 | 0.0001 | 0.0036 | 0.0008 | 0.0054 | 0 | 0.0024 | 0 | 0 | 0 | 0.016 | 0 | 0.0048 | 0.0001 | 0.0062 | 0.0001 |
Bud | 0.0024 | 0.0031 | 0.0006 | 0.0002 | 0.0002 | 0.0005 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0004 | 0.0004 | 0.0003 | 0.0004 | 0.0003 | 0.0002 |
Bud Neck | 0.0011 | 0.0015 | 0.0008 | 0.0007 | 0.0009 | 0.0014 | 0.0006 | 0.0006 | 0.0007 | 0.0003 | 0.0007 | 0.0007 | 0.0033 | 0.0008 | 0.0015 | 0.0008 | 0.0008 | 0.0007 |
Bud Periphery | 0.0017 | 0.0015 | 0.0009 | 0.0004 | 0.0005 | 0.0012 | 0.001 | 0.0007 | 0.0008 | 0.0002 | 0.0006 | 0.001 | 0.0011 | 0.0008 | 0.0008 | 0.0008 | 0.0007 | 0.0008 |
Bud Site | 0.0029 | 0.0037 | 0.0022 | 0.0004 | 0.0007 | 0.0006 | 0.0007 | 0.0004 | 0.0008 | 0.0001 | 0.0008 | 0.0004 | 0.0017 | 0.001 | 0.0008 | 0.0011 | 0.0004 | 0.0004 |
Cell Periphery | 0.0017 | 0.0025 | 0.0012 | 0.002 | 0.0009 | 0.003 | 0.0017 | 0.0023 | 0.0032 | 0.0016 | 0.0024 | 0.0026 | 0.0012 | 0.0025 | 0.0015 | 0.0014 | 0.0015 | 0.0017 |
Cytoplasm | 0.0101 | 0.0061 | 0.0035 | 0.0012 | 0.0014 | 0.0015 | 0.002 | 0.0036 | 0.0016 | 0.0005 | 0.0006 | 0.0012 | 0.0059 | 0.0029 | 0.0019 | 0.0019 | 0.0037 | 0.0011 |
Cytoplasmic Foci | 0.0241 | 0.0209 | 0.0241 | 0.0192 | 0.0029 | 0.0127 | 0.0062 | 0.0018 | 0.0049 | 0.0009 | 0.0007 | 0.0014 | 0.0089 | 0.0063 | 0.0078 | 0.0036 | 0.0061 | 0.0022 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0033 | 0.0023 | 0.0027 | 0.0008 | 0.0019 | 0.0022 | 0.0025 | 0.0031 | 0.0027 | 0.0003 | 0.0047 | 0.0022 | 0.0067 | 0.0045 | 0.0031 | 0.0018 | 0.0031 | 0.0018 |
Endosome | 0.3057 | 0.154 | 0.2428 | 0.1593 | 0.1151 | 0.1041 | 0.1843 | 0.0948 | 0.1577 | 0.0669 | 0.0787 | 0.1078 | 0.2007 | 0.1176 | 0.2036 | 0.1343 | 0.1719 | 0.1384 |
Golgi | 0.0198 | 0.01 | 0.0204 | 0.0234 | 0.0188 | 0.0113 | 0.0091 | 0.0079 | 0.0095 | 0.0008 | 0.0015 | 0.0043 | 0.0098 | 0.0034 | 0.0152 | 0.0029 | 0.0072 | 0.0058 |
Lipid Particles | 0.0524 | 0.008 | 0.0093 | 0.0187 | 0.01 | 0.0099 | 0.0208 | 0.0024 | 0.0136 | 0.0033 | 0.0032 | 0.005 | 0.027 | 0.0066 | 0.0145 | 0.0021 | 0.006 | 0.0089 |
Mitochondria | 0.043 | 0.0084 | 0.0333 | 0.0058 | 0.0114 | 0.0144 | 0.0218 | 0.0052 | 0.0227 | 0.0003 | 0.0098 | 0.0043 | 0.0298 | 0.0086 | 0.0274 | 0.0019 | 0.003 | 0.0163 |
None | 0.0038 | 0.001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.005 | 0.0006 | 0.0003 | 0.0001 | 0.0013 | 0.0001 |
Nuclear Periphery | 0.0159 | 0.0049 | 0.0041 | 0.0023 | 0.0046 | 0.005 | 0.0063 | 0.0091 | 0.0043 | 0.0008 | 0.0025 | 0.0042 | 0.0175 | 0.0106 | 0.0113 | 0.0023 | 0.0069 | 0.0121 |
Nucleolus | 0.0115 | 0.0085 | 0.0066 | 0.0058 | 0.0013 | 0.0083 | 0.0075 | 0.0008 | 0.0052 | 0.0008 | 0.0033 | 0.0015 | 0.0042 | 0.005 | 0.0024 | 0.002 | 0.0015 | 0.002 |
Nucleus | 0.0057 | 0.0039 | 0.0029 | 0.002 | 0.0052 | 0.0043 | 0.0054 | 0.0031 | 0.0022 | 0.0011 | 0.0035 | 0.0028 | 0.0049 | 0.0034 | 0.003 | 0.0025 | 0.0025 | 0.0038 |
Peroxisomes | 0.0058 | 0.0011 | 0.0024 | 0.0011 | 0.0011 | 0.0028 | 0.0017 | 0.0001 | 0.0007 | 0.0002 | 0.0003 | 0.0003 | 0.0068 | 0.0004 | 0.0022 | 0.0002 | 0.0007 | 0.0005 |
Punctate Nuclear | 0.0042 | 0.0003 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0014 | 0 | 0.0001 | 0 | 0 | 0 | 0.0039 | 0.0001 | 0.0003 | 0.0001 | 0.0011 | 0 |
Vacuole | 0.3273 | 0.5695 | 0.4322 | 0.6114 | 0.641 | 0.612 | 0.4626 | 0.6118 | 0.5065 | 0.702 | 0.6626 | 0.5978 | 0.4291 | 0.6159 | 0.4603 | 0.666 | 0.5969 | 0.555 |
Vacuole Periphery | 0.1435 | 0.1888 | 0.2085 | 0.1447 | 0.1783 | 0.2038 | 0.2578 | 0.2516 | 0.26 | 0.2199 | 0.2237 | 0.2619 | 0.2156 | 0.2086 | 0.237 | 0.1739 | 0.1781 | 0.2481 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 44.6547 | 24.037 | 16.5625 | 58.1932 | 34.2401 | 25.9142 | 29.7288 | 21.0901 | 40.9775 | 52.9443 |
Translational Efficiency | 0.7464 | 0.7013 | 0.7561 | 0.6441 | 0.8646 | 1.3428 | 0.5556 | 0.4787 | 0.7274 | 0.5865 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1386 | 1461 | 190 | 1370 | 1789 | 1583 | 2362 | 1037 | 3175 | 3044 | 2552 | 2407 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 934.49 | 843.00 | 970.98 | 1205.21 | 864.94 | 894.83 | 1000.47 | 1077.83 | 895.30 | 869.95 | 998.27 | 1150.33 |
Standard Deviation | 185.72 | 203.58 | 155.65 | 242.40 | 203.33 | 169.42 | 151.76 | 238.00 | 198.86 | 188.39 | 152.25 | 248.65 |
Intensity Change Log 2 | — | -0.148647 | 0.055262 | 0.367033 | — | 0.049014 | 0.210006 | 0.317458 | — | -0.050253 | 0.131717 | 0.343416 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000028 | 0.000108 | 0.000408 | 0.000077 | 0.000041 | 0.000039 | 0.000032 | 0.000701 | 0.000035 | 0.000072 | 0.000060 | 0.000346 |
Bud Neck | 0.003176 | 0.005647 | 0.020608 | 0.002854 | 0.004685 | 0.003059 | 0.006411 | 0.003157 | 0.004026 | 0.004302 | 0.007468 | 0.002985 |
Bud Site | 0.005748 | 0.021081 | 0.018458 | 0.008588 | 0.009581 | 0.004409 | 0.008094 | 0.019411 | 0.007908 | 0.012411 | 0.008865 | 0.013250 |
Cell Periphery | 0.007643 | 0.002487 | 0.000187 | 0.000406 | 0.003860 | 0.002863 | 0.000125 | 0.000649 | 0.005511 | 0.002683 | 0.000129 | 0.000510 |
Cytoplasm | 0.003025 | 0.023054 | 0.047488 | 0.006915 | 0.011782 | 0.008491 | 0.007783 | 0.013795 | 0.007959 | 0.015481 | 0.010739 | 0.009879 |
Cytoplasmic Foci | 0.000900 | 0.010584 | 0.033217 | 0.002555 | 0.007035 | 0.005880 | 0.012098 | 0.011309 | 0.004357 | 0.008138 | 0.013670 | 0.006326 |
Eisosomes | 0.000047 | 0.000025 | 0.000078 | 0.000009 | 0.000021 | 0.000023 | 0.000012 | 0.000010 | 0.000033 | 0.000024 | 0.000017 | 0.000009 |
Endoplasmic Reticulum | 0.004133 | 0.003863 | 0.005739 | 0.000917 | 0.003342 | 0.004584 | 0.002167 | 0.001110 | 0.003687 | 0.004238 | 0.002433 | 0.001000 |
Endosome | 0.010586 | 0.050670 | 0.099174 | 0.017778 | 0.042213 | 0.024117 | 0.138199 | 0.051837 | 0.028407 | 0.036862 | 0.135294 | 0.032451 |
Golgi | 0.000622 | 0.004598 | 0.007188 | 0.008260 | 0.006569 | 0.002031 | 0.012919 | 0.019852 | 0.003973 | 0.003263 | 0.012492 | 0.013254 |
Lipid Particles | 0.000316 | 0.000997 | 0.008727 | 0.000225 | 0.000654 | 0.000655 | 0.000960 | 0.000318 | 0.000507 | 0.000819 | 0.001538 | 0.000265 |
Mitochondria | 0.001876 | 0.002899 | 0.001179 | 0.001886 | 0.002573 | 0.001968 | 0.003277 | 0.004103 | 0.002269 | 0.002415 | 0.003121 | 0.002841 |
Mitotic Spindle | 0.000027 | 0.000780 | 0.004271 | 0.003355 | 0.000145 | 0.000144 | 0.004779 | 0.001660 | 0.000093 | 0.000449 | 0.004741 | 0.002625 |
None | 0.000080 | 0.002211 | 0.000375 | 0.000205 | 0.000782 | 0.000201 | 0.000219 | 0.000132 | 0.000476 | 0.001165 | 0.000231 | 0.000173 |
Nuclear Periphery | 0.000155 | 0.000203 | 0.003083 | 0.000822 | 0.000096 | 0.000187 | 0.001132 | 0.000162 | 0.000122 | 0.000195 | 0.001277 | 0.000538 |
Nuclear Periphery Foci | 0.000149 | 0.000728 | 0.007757 | 0.001085 | 0.000163 | 0.000116 | 0.002321 | 0.000306 | 0.000157 | 0.000410 | 0.002726 | 0.000749 |
Nucleolus | 0.000797 | 0.001907 | 0.009585 | 0.002167 | 0.000763 | 0.001450 | 0.003354 | 0.000550 | 0.000778 | 0.001669 | 0.003818 | 0.001470 |
Nucleus | 0.001499 | 0.007133 | 0.095263 | 0.002417 | 0.002985 | 0.006084 | 0.007831 | 0.001119 | 0.002336 | 0.006588 | 0.014341 | 0.001857 |
Peroxisomes | 0.000206 | 0.001280 | 0.004847 | 0.000203 | 0.000531 | 0.000590 | 0.000924 | 0.000527 | 0.000389 | 0.000921 | 0.001216 | 0.000343 |
Vacuole | 0.862957* | 0.735145* | 0.598919* | 0.620355* | 0.775873* | 0.887599* | 0.665355* | 0.620161* | 0.813888* | 0.814427* | 0.660409* | 0.620272* |
Vacuole Periphery | 0.096030 | 0.124598* | 0.033450 | 0.318924* | 0.126305* | 0.045511 | 0.122007* | 0.249133* | 0.113089* | 0.083470 | 0.115414* | 0.288856* |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.91 | -3.66 | -1.18 | 0.68 | 3.16 | 0.09 | 0.14 | -1.22 | -1.22 | -1.23 | -2.14 | -2.34 | -1.33 | -1.16 | -1.16 |
Bud Neck | -2.97 | -5.08 | 0.20 | 4.22 | 5.19 | 2.25 | -2.32 | 1.82 | -0.23 | 4.71 | -0.51 | -5.02 | 1.70 | 2.91 | 7.26 |
Bud Site | -7.26 | -2.96 | -2.31 | 5.74 | 2.19 | 5.34 | 1.28 | -4.36 | -6.74 | -4.97 | -3.96 | -1.17 | -4.52 | -0.84 | -3.49 |
Cell Periphery | 6.16 | 9.11 | 8.84 | 11.16 | -3.92 | 1.95 | 9.55 | 7.84 | 6.28 | -4.16 | 6.12 | 12.79 | 11.77 | 10.78 | -6.16 |
Cytoplasm | -7.69 | -6.70 | -3.22 | 5.00 | 5.93 | 1.52 | 1.75 | -1.44 | -2.67 | -2.89 | -4.53 | -2.79 | -2.32 | 2.04 | 0.24 |
Cytoplasmic Foci | -8.20 | -6.87 | -3.59 | 6.52 | 6.48 | 0.96 | -5.10 | -3.07 | -3.63 | 0.72 | -4.54 | -10.97 | -2.78 | 2.08 | 8.02 |
Eisosomes | 3.14 | -3.47 | 5.61 | 9.21 | 8.67 | -0.70 | 4.41 | 4.48 | 5.12 | 1.36 | 2.43 | 4.47 | 7.13 | 9.66 | 7.02 |
Endoplasmic Reticulum | 0.75 | -2.95 | 11.51 | 10.94 | 8.20 | -3.49 | 4.95 | 10.61 | 10.85 | 6.57 | -2.18 | 5.77 | 15.80 | 15.35 | 9.90 |
Endosome | -12.66 | -7.72 | -4.77 | 10.19 | 7.01 | 5.42 | -20.24 | -2.29 | -7.65 | 17.58 | -3.54 | -26.54 | -1.80 | 1.95 | 25.85* |
Golgi | -6.58 | -1.92 | -7.08 | -2.96 | -0.61 | 4.13 | -4.46 | -5.70 | -8.10 | -2.93 | 1.05 | -7.44 | -7.54 | -8.44 | -0.57 |
Lipid Particles | -3.71 | -5.58 | 1.05 | 4.51 | 5.65 | -0.01 | -2.06 | 2.50 | 1.86 | 4.69 | -2.30 | -5.79 | 2.95 | 4.51 | 7.56 |
Mitochondria | -1.39 | 1.06 | -0.04 | 1.26 | -1.05 | 0.87 | -1.21 | -1.21 | -1.72 | -0.66 | -0.30 | -1.74 | -0.79 | -0.62 | 0.42 |
Mitotic Spindle | -1.53 | -2.23 | -4.00 | -2.71 | 0.33 | 0.02 | -5.68 | -2.87 | -2.88 | 3.38 | -1.49 | -6.03 | -4.81 | -3.80 | 2.38 |
None | -3.29 | -2.46 | -2.23 | 3.07 | 1.17 | 3.82 | 3.61 | 4.40 | 1.19 | 1.56 | -2.15 | 2.47 | 3.30 | 3.13 | 0.96 |
Nuclear Periphery | -0.94 | -5.39 | -3.88 | -3.62 | 3.95 | -2.43 | -8.78 | -2.00 | 0.68 | 8.29 | -2.36 | -9.88 | -4.21 | -3.50 | 5.07 |
Nuclear Periphery Foci | -1.51 | -3.98 | -4.09 | -0.79 | 3.59 | 1.38 | -4.81 | -2.34 | -3.40 | 4.50 | -1.37 | -5.98 | -4.44 | -1.48 | 4.59 |
Nucleolus | -3.37 | -3.93 | -3.08 | -0.72 | 3.21 | -1.99 | -8.30 | 1.64 | 2.57 | 8.82 | -3.78 | -8.92 | -2.80 | 0.44 | 5.78 |
Nucleus | -4.75 | -7.35 | -1.83 | 3.70 | 7.27 | -2.70 | -5.16 | 2.79 | 4.77 | 7.93 | -5.11 | -9.10 | 0.71 | 5.71 | 9.44 |
Peroxisomes | -3.58 | -4.89 | -0.03 | 3.61 | 4.89 | -0.35 | -3.76 | 0.05 | 0.36 | 3.17 | -3.14 | -7.34 | 0.60 | 3.39 | 7.68 |
Vacuole | 15.35 | 12.68 | 30.10 | 12.20 | -1.32 | -14.83 | 14.72 | 15.77 | 28.65 | 4.67 | -0.01 | 24.85 | 30.26 | 29.19 | 5.52 |
Vacuole Periphery | -4.83 | 9.66 | -28.99* | -24.95* | -33.74* | 18.07* | 1.12 | -15.01 | -27.13* | -16.67* | 7.95 | -0.29 | -31.14 | -37.63* | -31.64* |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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