Standard name
Human Ortholog
Description Cytosolic aldehyde dehydrogenase; activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.09 0 0 0 0 0 0 0 0 0.05 0 0.23 0.27 0.3 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.96 0.98 0.99 0.98 0.98 0.97 0.97 0.95 0.94 0.91 0.93 0.84 0.81 0.73 0.98 0.99 0.97 0.95 0.96 0.93 0.95 0.95 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.1 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 1 3 1 1 2 0 2 4 0 1 0 1 1 2 1 0 0 0 1 0 2 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 32 2 6 3 5 7 3 5 8 11 8 46 79 85 1 0 0 0 3 0 0 0 0
Cytoplasm 258 358 294 247 254 266 320 285 207 252 184 210 166 239 205 120 83 66 235 417 268 141 234 196
Endoplasmic Reticulum 1 0 2 2 0 0 5 3 6 11 11 7 0 0 1 5 8 6 0 1 4 1 0 5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 2 0 2 1 0 2 0 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0
Mitochondria 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 0 0 1 0 2 0 0 0 2 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 1 0 0 0 1 0 0 0 0 0 2 0 0 0 0 1 2 1 2 3
Unique Cell Count 260 373 300 250 258 272 329 294 217 269 202 226 197 295 280 122 84 68 248 435 288 149 246 221
Labelled Cell Count 262 393 302 257 262 273 334 296 219 274 206 228 213 320 295 128 92 73 248 435 288 149 246 221


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 245.8 430.5 253.4 327.2 299.2 261.0 232.1 209.2 213.3 197.3 201.4 196.6 514.6 503.4 513.2 235.7 293.7 293.8 303.0 323.5 329.5
Std Deviation (1e-4) 69.5 101.2 75.0 96.6 83.1 76.5 63.5 67.7 72.0 61.4 63.9 61.5 127.0 117.3 133.3 103.6 114.6 86.0 92.1 94.6 110.4
Intensity Change (Log2) – – – 0.37 0.24 0.04 -0.13 -0.28 -0.25 -0.36 -0.33 -0.37 1.02 0.99 1.02 -0.1 0.21 0.21 0.26 0.35 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 3.3 0 8.4 9.3 10.0 0 0 0
Cytoplasm 0.7 0.4 -0.2 -0.6 -0.8 -1.7 -2.6 -3.6 -2.9 -5.7 -6.8 -8.6 0.2 0.5 -0.5
Endoplasmic Reticulum 0 0 0 0 0 0 2.7 3.3 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 530.6914 690.4981 588.0433 516.7783 529.2042 551.5987 523.3662 683.2597 566.6449 535.248 609.8973 527.7108 486.4275 697.0778 553.1838 490.6731 475.3605 586.5997
Actin 0.0007 0.0002 0.0006 0.0001 0.0004 0.0006 0.007 0.0009 0.0018 0.0004 0.0001 0.0003 0.0011 0.0002 0.0203 0.0001 0.0007 0.0002
Bud 0.0031 0.0002 0.0002 0.0003 0.0005 0.0003 0.0028 0.0007 0.0005 0.0002 0.0002 0.003 0.0045 0.0003 0.0023 0.0001 0.0005 0.001
Bud Neck 0.0003 0.0001 0.0002 0.0002 0.0003 0.0008 0.0002 0.0001 0.0002 0.0002 0.0002 0.0004 0.0003 0.0001 0.0003 0.0001 0.0005 0.0003
Bud Periphery 0.0017 0 0.0001 0 0.0001 0 0.0034 0.0003 0.0002 0 0 0.0005 0.002 0 0.0006 0 0.0001 0.0001
Bud Site 0.0005 0.0001 0.0001 0 0.0001 0 0.0012 0.0003 0.0005 0 0 0.0002 0.0005 0.0002 0.0007 0 0.0001 0
Cell Periphery 0.0049 0.0003 0.0012 0.0002 0.0004 0.0001 0.001 0.0011 0.0005 0 0.0002 0.0003 0.001 0.0005 0.0006 0 0.001 0
Cytoplasm 0.8094 0.9518 0.89 0.8991 0.8277 0.87 0.9149 0.9694 0.879 0.9716 0.9534 0.8932 0.8327 0.972 0.8772 0.9124 0.7933 0.9322
Cytoplasmic Foci 0.0063 0.0001 0.0141 0.0002 0.0138 0.0002 0.0014 0.0003 0.0009 0.0002 0.0002 0.0007 0.0165 0.0015 0.0052 0.0001 0.028 0.0006
Eisosomes 0.0003 0 0.0004 0 0.0008 0 0.0003 0.0009 0.0006 0 0 0 0.0006 0.0001 0.002 0 0.0008 0
Endoplasmic Reticulum 0.0012 0.0001 0.0001 0.0001 0.0002 0.0019 0.0004 0.0001 0.0005 0.0001 0.0001 0.0001 0.0005 0.0001 0.0005 0.0001 0.0002 0.0001
Endosome 0.0002 0 0.0002 0.0001 0.0001 0.0015 0.0002 0 0.0004 0 0 0 0.0023 0 0.0019 0 0.0003 0
Golgi 0.0001 0 0.0003 0 0 0 0.0001 0 0.0001 0 0 0 0.0003 0 0.0025 0 0.0002 0
Lipid Particles 0.0008 0 0.0069 0 0.0014 0 0.0001 0.0001 0.0001 0 0 0 0.0052 0.0001 0.0036 0 0.0097 0
Mitochondria 0.0173 0.002 0.0083 0.0013 0.0111 0.0019 0.0106 0.0106 0.0036 0.0004 0.0004 0.0272 0.0107 0.0005 0.0172 0.0006 0.0109 0.0043
None 0.0083 0.0011 0.0019 0.001 0.005 0.0007 0.005 0.0017 0.007 0.001 0.0006 0.0016 0.0164 0.0019 0.0129 0.0006 0.0182 0.003
Nuclear Periphery 0.0111 0.003 0.0058 0.0116 0.0069 0.0185 0.0053 0.0017 0.0114 0.0022 0.0069 0.0055 0.0102 0.0014 0.007 0.0091 0.0063 0.0022
Nucleolus 0.022 0.0046 0.0247 0.0011 0.0875 0.0005 0.0025 0.0016 0.0234 0.0001 0.0007 0.0132 0.0271 0.0023 0.0083 0.0002 0.0838 0.0017
Nucleus 0.1014 0.0332 0.0352 0.0787 0.0397 0.0998 0.0399 0.0083 0.0616 0.0231 0.0339 0.0512 0.0567 0.0166 0.0273 0.0759 0.0256 0.0529
Peroxisomes 0.0005 0 0.0053 0 0.0005 0 0.0002 0.0003 0.0001 0 0 0.0003 0.0022 0 0.0012 0 0.0007 0.0001
Punctate Nuclear 0.0007 0.0001 0.0009 0.0002 0.0007 0.0004 0.0005 0.0002 0.0042 0.0004 0.0002 0.0002 0.0028 0.0018 0.0051 0.0002 0.0009 0.0003
Vacuole 0.0071 0.0014 0.0015 0.0031 0.0011 0.0013 0.0016 0.0006 0.0019 0.0001 0.0018 0.0012 0.004 0.0005 0.0015 0.0003 0.0051 0.0005
Vacuole Periphery 0.0021 0.0015 0.0019 0.0025 0.0016 0.0016 0.0014 0.0005 0.0015 0.0001 0.0012 0.0009 0.0026 0.0001 0.002 0.0002 0.0132 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1885.992 1041.503 1423.9349 1613.7008 543.9753 1997.9421 1638.9821 1715.9574 2024.8577 745.0877
Translational Efficiency 1.5424 1.5725 1.5425 1.8446 1.702 1.6399 1.735 1.7535 1.6397 1.6943

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1983 748 2055 1246 1435 1128 1613 33 3418 1876 3668 1279

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 9960.01 22630.67 29662.38 25513.53 15693.57 13272.62 31166.88 29161.52 12367.17 17003.87 30323.98 25607.65
Standard Deviation 3981.24 7105.71 8628.51 9174.15 4335.41 4861.38 8349.29 8959.78 5009.40 7438.88 8539.57 9186.91
Intensity Change Log 2 — 1.184060 1.574415 1.357043 — -0.241720 0.989840 0.893892 — 0.484956 1.245605 1.091722

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000063 0.000112 0.000077 0.000272 0.000043 0.000095 0.000061 0.000099 0.000054 0.000102 0.000070 0.000268
Bud Neck 0.000980 0.000642 0.001040 0.001496 0.000336 0.000531 0.000781 0.003514 0.000710 0.000575 0.000926 0.001548
Bud Site 0.005035 0.002353 0.002051 0.008512 0.000452 0.001701 0.000675 0.001667 0.003111 0.001961 0.001446 0.008336
Cell Periphery 0.002057 0.002283 0.006480 0.012501 0.002889 0.000859 0.002172 0.012309 0.002406 0.001427 0.004585 0.012496
Cytoplasm 0.837672* 0.682448* 0.773428* 0.787501* 0.791346* 0.745293* 0.785571* 0.444751* 0.818222* 0.720235* 0.778768* 0.778658*
Cytoplasmic Foci 0.008740 0.007444 0.002008 0.008948 0.005625 0.006139 0.002994 0.041593 0.007432 0.006659 0.002442 0.009791
Eisosomes 0.000001 0.000001 0.000001 0.000007 0.000001 0.000001 0.000001 0.000005 0.000001 0.000001 0.000001 0.000007
Endoplasmic Reticulum 0.000064 0.000022 0.000086 0.000076 0.000024 0.000034 0.000020 0.000026 0.000047 0.000029 0.000057 0.000074
Endosome 0.000122 0.000034 0.000080 0.000912 0.000027 0.000019 0.000029 0.000195 0.000082 0.000025 0.000058 0.000894
Golgi 0.002836 0.000995 0.000551 0.001282 0.002360 0.000396 0.000630 0.013488 0.002636 0.000635 0.000586 0.001597
Lipid Particles 0.000433 0.001114 0.001566 0.005365 0.001488 0.000303 0.001042 0.014045 0.000876 0.000626 0.001336 0.005589
Mitochondria 0.000384 0.000201 0.000212 0.000327 0.000179 0.000257 0.000176 0.000039 0.000298 0.000235 0.000196 0.000319
Mitotic Spindle 0.002860 0.000406 0.000208 0.001149 0.000177 0.000589 0.000321 0.000210 0.001733 0.000516 0.000257 0.001125
None 0.011034 0.011690 0.005325 0.012037 0.010767 0.018536 0.006375 0.000627 0.010922 0.015806 0.005787 0.011743
Nuclear Periphery 0.000204 0.000203 0.000147 0.000185 0.000210 0.000100 0.000267 0.001461 0.000207 0.000141 0.000200 0.000218
Nuclear Periphery Foci 0.001103 0.000932 0.000515 0.001470 0.001235 0.000401 0.000666 0.013713 0.001158 0.000613 0.000581 0.001786
Nucleolus 0.000144 0.000068 0.000162 0.000304 0.000044 0.000147 0.000094 0.000105 0.000102 0.000115 0.000132 0.000299
Nucleus 0.113707 0.278838* 0.109153 0.076866 0.174015* 0.220349* 0.166412* 0.062890 0.139027* 0.243670* 0.134333* 0.076506
Peroxisomes 0.000018 0.000006 0.000018 0.000905 0.000002 0.000011 0.000006 0.000018 0.000012 0.000009 0.000012 0.000882
Vacuole 0.005051 0.009530 0.095028 0.078985 0.006815 0.003995 0.030261 0.377838* 0.005792 0.006202 0.066547 0.086696
Vacuole Periphery 0.007492 0.000680 0.001864 0.000899 0.001965 0.000246 0.001445 0.011407 0.005172 0.000419 0.001680 0.001170

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.91 -4.28 -7.96 -3.61 -6.69 -2.10 -3.48 -3.35 0.06 -1.08 -2.67 -5.40 -8.34 -4.98 -7.02
Bud Neck -0.25 -6.24 -6.63 -4.79 0.94 -1.77 -7.35 -5.34 -5.06 -4.10 -0.56 -8.87 -9.49 -8.06 -1.06
Bud Site 2.07 3.57 -3.92 -5.00 -6.85 -2.76 -5.35 -3.90 -1.86 -3.11 1.40 2.88 -6.21 -6.65 -7.73
Cell Periphery -1.07 -4.67 -7.10 -6.54 -3.90 5.60 2.32 -1.02 -2.53 -1.75 3.25 -2.93 -6.80 -7.86 -5.45
Cytoplasm 17.62 15.83 10.43 -8.46 -3.44 6.57 8.25 9.22 7.78 7.51 17.91 17.54 9.32 -5.00 -2.90
Cytoplasmic Foci -1.09 5.23 -2.47 -1.36 -5.80 -2.02 -0.01 -1.75 -1.68 -1.75 -1.44 4.36 -3.86 -3.02 -5.61
Eisosomes -4.91 -8.26 -6.80 -5.55 -5.32 -1.48 -4.08 -4.23 -4.00 -3.69 -4.56 -9.01 -6.86 -6.12 -5.67
Endoplasmic Reticulum 3.41 -1.27 0.50 -1.70 1.46 -0.45 0.42 1.42 0.85 1.50 1.49 -1.01 -0.31 -1.22 0.15
Endosome 3.36 -0.54 -1.43 -1.71 -1.39 0.80 -2.55 -3.23 -3.33 -2.78 4.22 -0.99 -1.59 -1.78 -1.54
Golgi 4.93 6.41 4.37 0.15 -0.90 6.09 5.38 -0.59 -1.99 -1.83 8.05 8.32 4.60 -1.15 -1.12
Lipid Particles -2.94 -6.96 -10.44 -8.18 -7.60 2.52 0.12 -2.97 -3.46 -3.02 0.50 -3.54 -9.53 -9.97 -8.33
Mitochondria 5.85 4.02 1.56 -1.48 -0.66 -1.99 -0.38 9.99 5.73 4.48 2.43 3.08 0.29 -0.85 -1.01
Mitotic Spindle 3.26 3.47 2.46 -1.49 -2.01 -1.35 -2.49 1.68 1.53 3.07 2.18 3.05 1.53 -0.82 -1.79
None 0.28 7.88 1.51 0.93 -2.42 -3.35 6.44 17.19 8.70 7.52 -2.72 10.14 1.62 3.13 -2.17
Nuclear Periphery -0.81 -1.38 -0.73 0.20 1.33 4.84 -2.21 -4.09 -4.74 -2.75 0.97 -2.50 -1.29 -3.59 1.74
Nuclear Periphery Foci 0.04 1.13 -1.93 -2.68 -5.25 4.16 1.36 -2.35 -2.91 -2.58 2.00 1.60 -2.64 -5.34 -5.03
Nucleolus 3.93 -4.38 -1.20 -2.20 0.05 -2.57 -8.92 -2.97 0.36 -0.33 -0.69 -6.76 -1.87 -1.50 -0.66
Nucleus -18.78 -5.43 4.84 21.49 10.05 -6.97 -4.75 2.11 3.96 3.32 -19.78 -7.39 11.10 27.35 17.48
Peroxisomes 2.10 -0.06 -1.25 -1.27 -1.25 -2.86 -5.94 -2.77 -1.60 -2.22 0.41 -1.08 -1.27 -1.27 -1.26
Vacuole -4.53 -23.62 -20.79 -16.46 1.24 1.19 -12.64 -7.57 -7.61 -6.74 -3.29 -26.39 -21.13 -19.89 -4.77
Vacuole Periphery 5.71 4.78 5.73 -0.03 5.24 3.69 1.44 -0.44 -1.89 -1.13 6.63 4.99 6.13 -1.70 3.35
Standard name
Human Ortholog
Description Cytosolic aldehyde dehydrogenase; activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Ald6

Ald6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ald6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available