Standard name | |
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Human Ortholog | |
Description | Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 0 | 4 | 5 | 3 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 12 | 8 | 6 | 14 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9 | 19 | 4 | 3 | 8 | 4 | 18 | 17 | 16 | 15 | 16 | 19 | 9 | 34 | 35 | 29 | 19 | 22 | 4 | 5 | 0 | 6 | 2 | 7 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 9 | 10 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 0 | 2 | 0 | 7 | 0 | 0 | 1 | 2 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 20 | 207 | 3 | 6 | 4 | 13 | 69 | 22 | 122 | 69 | 114 | 175 | 5 | 4 | 2 | 15 | 14 | 8 | 2 | 11 | 0 | 1 | 2 | 7 |
Nucleus | 262 | 328 | 92 | 113 | 153 | 39 | 85 | 124 | 120 | 122 | 211 | 231 | 116 | 182 | 205 | 222 | 133 | 160 | 254 | 355 | 101 | 172 | 132 | 129 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 2 | 3 | 4 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 1 | 1 | 1 | 2 | 0 | 2 | 0 | 3 | 1 | 7 | 0 | 2 | 3 | 0 | 1 | 1 | 0 | 7 | 0 | 2 | 3 | 3 |
Vac/Vac Membrane | 10 | 7 | 1 | 2 | 3 | 0 | 1 | 11 | 4 | 1 | 5 | 9 | 1 | 9 | 3 | 23 | 20 | 15 | 12 | 49 | 9 | 21 | 20 | 44 |
Unique Cell Count | 282 | 415 | 98 | 121 | 167 | 46 | 134 | 162 | 177 | 155 | 270 | 320 | 128 | 223 | 244 | 269 | 176 | 201 | 281 | 446 | 112 | 210 | 172 | 207 |
Labelled Cell Count | 302 | 565 | 102 | 127 | 171 | 61 | 185 | 185 | 280 | 227 | 359 | 467 | 131 | 233 | 252 | 306 | 198 | 216 | 281 | 446 | 112 | 210 | 172 | 207 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 3.7 | 5.5 | 5.3 | 5.9 | 4.6 | 3.4 | 5.2 | 3.8 | 4.3 | 4.2 | 4.1 | 5.4 | 5.5 | 5.4 | 4.9 | 5.9 | 6.5 | 5.7 | 6.2 | 6.2 |
Std Deviation (1e-4) | 0.9 | 0.7 | 1.0 | 1.3 | 1.3 | 1.2 | 1.1 | 1.7 | 1.1 | 1.2 | 1.3 | 0.8 | 0.9 | 1.3 | 1.1 | 1.2 | 1.3 | 1.7 | 1.0 | 1.4 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.1 | -0.26 | -0.68 | -0.07 | -0.55 | -0.35 | -0.39 | -0.43 | -0.04 | -0.0 | -0.04 | -0.16 | 0.09 | 0.24 | 0.04 | 0.17 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.6 | 3.1 | 2.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.4222 | 4.4672 | 4.4912 | 4.1159 | 3.8727 | 4.3684 | 4.1423 | 4.665 | 3.9241 | 4.156 | 4.1738 | 4.0224 | 2.2997 | 2.0175 | 1.9613 | 2.181 | 1.6801 | 1.7932 |
Actin | 0.0441 | 0.0001 | 0.0124 | 0.0014 | 0.0233 | 0.0114 | 0.02 | 0.0001 | 0.0017 | 0 | 0.0016 | 0.0001 | 0.0587 | 0.0001 | 0.0126 | 0.0398 | 0.0021 | 0.0043 |
Bud | 0.0004 | 0 | 0.0002 | 0 | 0.0004 | 0.0003 | 0.0003 | 0 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.001 | 0 | 0.0003 | 0.0006 | 0.0001 | 0.0002 |
Bud Neck | 0.006 | 0.0001 | 0.0012 | 0.0002 | 0.001 | 0.0015 | 0.0024 | 0.0001 | 0.0046 | 0.0001 | 0.0003 | 0.0009 | 0.0192 | 0.0003 | 0.0006 | 0.0016 | 0.0007 | 0.0012 |
Bud Periphery | 0.0006 | 0 | 0.0003 | 0.0001 | 0.0028 | 0.0013 | 0.0006 | 0 | 0.0007 | 0 | 0.0005 | 0.0001 | 0.0012 | 0 | 0.0004 | 0.0023 | 0.0001 | 0.0004 |
Bud Site | 0.0118 | 0.0001 | 0.005 | 0.0001 | 0.0004 | 0.0014 | 0.0066 | 0 | 0.0057 | 0 | 0.0006 | 0.0001 | 0.0287 | 0.0002 | 0.0066 | 0.0049 | 0.0001 | 0.0005 |
Cell Periphery | 0.0006 | 0 | 0.0008 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0.0029 | 0 | 0.0001 |
Cytoplasm | 0.0043 | 0.0012 | 0.0069 | 0.0004 | 0.0002 | 0.0023 | 0.0092 | 0.0031 | 0.003 | 0.0001 | 0.0045 | 0.0006 | 0.0197 | 0.0112 | 0.003 | 0.0143 | 0.0033 | 0.0018 |
Cytoplasmic Foci | 0.0149 | 0 | 0.003 | 0.0005 | 0.0014 | 0.0015 | 0.0193 | 0.0003 | 0.0023 | 0 | 0.0111 | 0.0007 | 0.026 | 0.0003 | 0.0118 | 0.0013 | 0.001 | 0.0027 |
Eisosomes | 0.0008 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0.0004 | 0 | 0 |
Endoplasmic Reticulum | 0.0022 | 0.0005 | 0.0017 | 0 | 0.0007 | 0.0041 | 0.0026 | 0.0001 | 0.0008 | 0 | 0.0044 | 0 | 0.0037 | 0.0008 | 0.0002 | 0.0093 | 0.002 | 0.0005 |
Endosome | 0.0236 | 0.0001 | 0.006 | 0.0009 | 0.0039 | 0.0087 | 0.0093 | 0.0001 | 0.0015 | 0 | 0.0243 | 0.0003 | 0.0198 | 0.0009 | 0.0047 | 0.0021 | 0.017 | 0.0059 |
Golgi | 0.0103 | 0 | 0.0053 | 0.0014 | 0.0064 | 0.0021 | 0.0031 | 0 | 0.0006 | 0 | 0.0032 | 0.0003 | 0.0123 | 0 | 0.0041 | 0.0057 | 0.0008 | 0.0025 |
Lipid Particles | 0.0139 | 0 | 0.0029 | 0.0013 | 0.007 | 0.0013 | 0.0057 | 0.0001 | 0.0016 | 0 | 0.0088 | 0.0044 | 0.0184 | 0.0001 | 0.0093 | 0.002 | 0.0004 | 0.0052 |
Mitochondria | 0.0033 | 0.0005 | 0.0028 | 0.0302 | 0.0083 | 0.003 | 0.0018 | 0.0002 | 0.003 | 0.0001 | 0.0114 | 0.0004 | 0.0086 | 0.0003 | 0.0211 | 0.0014 | 0.0014 | 0.0074 |
None | 0.0376 | 0.0061 | 0.0138 | 0.0002 | 0.0003 | 0.0006 | 0.0289 | 0.0655 | 0.0075 | 0 | 0.0006 | 0.0052 | 0.0123 | 0.0169 | 0.0017 | 0.0039 | 0.0018 | 0.0002 |
Nuclear Periphery | 0.0057 | 0.0116 | 0.0095 | 0.0014 | 0.0143 | 0.0072 | 0.0082 | 0.0086 | 0.0054 | 0.0003 | 0.0064 | 0.0005 | 0.0125 | 0.0083 | 0.0018 | 0.0033 | 0.0245 | 0.0019 |
Nucleolus | 0.0055 | 0.0072 | 0.0049 | 0.0076 | 0.0197 | 0.0092 | 0.0078 | 0.0051 | 0.0044 | 0.0044 | 0.0038 | 0.0091 | 0.0056 | 0.0054 | 0.0041 | 0.0024 | 0.0091 | 0.0097 |
Nucleus | 0.7932 | 0.9681 | 0.9155 | 0.9306 | 0.8887 | 0.9311 | 0.841 | 0.9087 | 0.9455 | 0.9946 | 0.8673 | 0.9677 | 0.6762 | 0.9505 | 0.9052 | 0.8947 | 0.9292 | 0.9497 |
Peroxisomes | 0.0105 | 0 | 0.0042 | 0.0022 | 0.0037 | 0.0014 | 0.0081 | 0 | 0.0033 | 0 | 0.043 | 0.0052 | 0.0449 | 0 | 0.0095 | 0.0015 | 0.0002 | 0.0032 |
Punctate Nuclear | 0.0086 | 0.0037 | 0.0022 | 0.0211 | 0.0113 | 0.0061 | 0.0208 | 0.0076 | 0.0057 | 0.0004 | 0.0029 | 0.0039 | 0.0277 | 0.0038 | 0.0014 | 0.0045 | 0.0037 | 0.0013 |
Vacuole | 0.0012 | 0.0002 | 0.0011 | 0.0001 | 0.0017 | 0.0039 | 0.0029 | 0.0002 | 0.0015 | 0 | 0.0031 | 0.0002 | 0.002 | 0.0006 | 0.0011 | 0.001 | 0.0011 | 0.0009 |
Vacuole Periphery | 0.0006 | 0.0002 | 0.0004 | 0.0001 | 0.0041 | 0.0014 | 0.0007 | 0.0001 | 0.0003 | 0 | 0.0019 | 0 | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.0013 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.0057 | 3.2661 | 12.926 | 18.7102 | 17.3695 | 12.1721 | 14.5856 | 21.9175 | 22.2998 | 17.6117 |
Translational Efficiency | 0.5585 | 2.7396 | 0.7783 | 0.4797 | 0.499 | 0.7851 | 0.6704 | 0.5746 | 0.5389 | 0.619 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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