Standard name | |
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Human Ortholog | |
Description | ATPase component of heat shock protein Hsp90 chaperone complex; serves as nucleotide exchange factor to load ATP onto the SSA class of cytosolic Hsp70s; plays a role in prion propagation and determining prion variants; binds unfolded proteins; member of Hsp110 subclass of HSP70 proteins; deletion results in spindle elongation in S phase; SSE1 has a paralog, SSE2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 2 | 3 | 2 | 1 | 3 | 2 | – | 2 | 12 | 18 | 6 | 1 | 0 | 1 | 3 | 1 | 0 | 0 | 2 | 2 | 1 | 2 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 13 | 12 | 16 | 24 | 30 | – | 30 | 44 | 25 | 43 | 2 | 0 | 2 | 62 | 44 | 28 | 1 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 159 | 188 | 312 | 375 | 502 | 528 | 709 | – | 499 | 639 | 584 | 584 | 331 | 427 | 469 | 761 | 472 | 564 | 157 | 189 | 309 | 228 | 326 | 425 |
Endoplasmic Reticulum | 1 | 0 | 0 | 4 | 9 | 15 | 43 | – | 42 | 59 | 65 | 64 | 3 | 8 | 2 | 22 | 19 | 26 | 1 | 0 | 1 | 9 | 17 | 25 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 2 | 2 | 3 | 4 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 1 | 0 | 0 | – | 1 | 2 | 3 | 1 | 1 | 0 | 1 | 8 | 2 | 3 | 0 | 0 | 2 | 9 | 4 | 7 |
Unique Cell Count | 160 | 189 | 315 | 381 | 513 | 546 | 756 | 553 | 713 | 663 | 676 | 335 | 434 | 471 | 787 | 490 | 571 | 164 | 197 | 324 | 257 | 365 | 474 | |
Labelled Cell Count | 160 | 192 | 328 | 394 | 529 | 570 | 784 | 575 | 758 | 700 | 700 | 338 | 436 | 476 | 860 | 544 | 621 | 164 | 197 | 324 | 257 | 365 | 474 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 117.8 | 98.4 | 98.6 | 100.8 | 100.8 | 87.9 | 84.0 | – | 78.1 | 73.0 | 68.0 | 83.2 | 125.0 | 127.1 | 121.9 | 90.4 | 94.2 | 87.3 | 103.8 | 104.5 | 111.4 |
Std Deviation (1e-4) | 31.2 | 31.6 | 26.2 | 26.5 | 22.0 | 20.0 | 19.2 | – | 20.7 | 19.2 | 19.3 | 22.2 | 31.0 | 35.5 | 36.7 | 29.0 | 30.5 | 25.9 | 26.7 | 32.0 | 30.9 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.03 | -0.17 | -0.23 | – | -0.34 | -0.43 | -0.54 | -0.24 | 0.34 | 0.37 | 0.31 | -0.13 | -0.07 | -0.18 | 0.07 | 0.08 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.7 | -0.8 | 0.2 | -0.1 | – | 0.8 | 1.3 | -0.3 | 1.4 | -3.0 | -4.3 | -3.7 | 2.2 | 2.6 | 0.5 |
Cytoplasm | -0.7 | -1.3 | -2.1 | -3.7 | – | -5.0 | -5.3 | -5.8 | -6.3 | -0.3 | -0.8 | 0.9 | -2.2 | -2.4 | -0.4 |
Endoplasmic Reticulum | 0 | 0 | 3.0 | 4.3 | – | 5.0 | 5.3 | 5.8 | 5.6 | 0 | 0 | 0 | 3.0 | 3.5 | 3.8 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0105 | 0.0001 | 0.0005 | 0.0029 | 0.0021 | 0.002 | 0.0006 | 0.0005 | 0.0003 | 0.0001 | 0.0014 | 0.0001 | 0.0826 | 0.0009 | 0.0171 | 0.0001 | 0 | 0.0005 |
Bud | 0.0019 | 0.0003 | 0.0011 | 0.0033 | 0.0037 | 0.0021 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.0022 | 0.0001 | 0.0033 | 0.0003 | 0.0008 | 0.0001 | 0.0001 | 0.0003 |
Bud Neck | 0.001 | 0.0002 | 0.0004 | 0.0011 | 0.0014 | 0.0086 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0009 | 0.0006 | 0.0013 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0004 |
Bud Periphery | 0.0024 | 0.0001 | 0.0005 | 0.002 | 0.0055 | 0.0011 | 0.0002 | 0 | 0 | 0 | 0.0019 | 0 | 0.0031 | 0.0001 | 0.0002 | 0 | 0 | 0 |
Bud Site | 0.0037 | 0.0002 | 0.0005 | 0.0013 | 0.0005 | 0.0008 | 0.0007 | 0.0007 | 0.0004 | 0 | 0.0004 | 0 | 0.002 | 0.0038 | 0.0011 | 0 | 0 | 0 |
Cell Periphery | 0.0054 | 0.0025 | 0.0012 | 0.011 | 0.0005 | 0.0031 | 0.0018 | 0.0013 | 0.0004 | 0.0001 | 0.0004 | 0 | 0.0007 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.7588 | 0.9849 | 0.9651 | 0.8995 | 0.9307 | 0.9393 | 0.8809 | 0.9896 | 0.9602 | 0.9881 | 0.6857 | 0.9951 | 0.0098 | 0.9928 | 0.9596 | 0.9913 | 0.9966 | 0.9875 |
Cytoplasmic Foci | 0.0151 | 0.0002 | 0.0046 | 0.0017 | 0.0008 | 0.0014 | 0.0021 | 0.0006 | 0.0032 | 0.0004 | 0.004 | 0.0002 | 0.013 | 0.0005 | 0.0049 | 0.0021 | 0.0013 | 0.0031 |
Eisosomes | 0.0026 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0148 | 0.001 | 0.0038 | 0.0027 | 0.0015 | 0.0027 | 0.0029 | 0.0002 | 0.0004 | 0.0003 | 0.0103 | 0.0004 | 0.0336 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0018 |
Endosome | 0.0133 | 0.0001 | 0.0008 | 0.0056 | 0.0052 | 0.0016 | 0.0028 | 0.0002 | 0.0003 | 0.0002 | 0.1482 | 0.0001 | 0.3699 | 0.0002 | 0.0053 | 0.002 | 0.0007 | 0.0026 |
Golgi | 0.0069 | 0 | 0.0001 | 0.0006 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0183 | 0 | 0.4029 | 0.0001 | 0.004 | 0 | 0 | 0.0002 |
Lipid Particles | 0.0097 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0.001 | 0 | 0.0005 | 0 | 0.0042 | 0 | 0.0002 | 0 | 0 | 0 |
Mitochondria | 0.0343 | 0.0001 | 0.0024 | 0.0011 | 0.0083 | 0.0011 | 0.0015 | 0.0001 | 0.0231 | 0 | 0.0079 | 0 | 0.031 | 0 | 0.0021 | 0.0001 | 0 | 0.0003 |
None | 0.0134 | 0.0002 | 0.0011 | 0.0006 | 0.0003 | 0.0003 | 0.0047 | 0.0011 | 0.0026 | 0.0002 | 0.0003 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0003 |
Nuclear Periphery | 0.0262 | 0.0023 | 0.0019 | 0.0067 | 0.0066 | 0.0047 | 0.045 | 0.0009 | 0.0009 | 0.0012 | 0.0099 | 0.0011 | 0.0134 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0007 |
Nucleolus | 0.0143 | 0.0001 | 0.0035 | 0.0007 | 0.0004 | 0.0004 | 0.0044 | 0.001 | 0.0013 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.029 | 0.0027 | 0.003 | 0.0095 | 0.0098 | 0.0085 | 0.0305 | 0.0013 | 0.0017 | 0.0021 | 0.0023 | 0.0014 | 0.0012 | 0.0003 | 0.0006 | 0.0004 | 0.0003 | 0.0007 |
Peroxisomes | 0.001 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0004 | 0 | 0 | 0 |
Punctate Nuclear | 0.0024 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0 |
Vacuole | 0.0244 | 0.0045 | 0.0076 | 0.0432 | 0.0143 | 0.0169 | 0.0154 | 0.0014 | 0.0022 | 0.0056 | 0.0542 | 0.0006 | 0.0102 | 0.0002 | 0.0016 | 0.0021 | 0.0005 | 0.001 |
Vacuole Periphery | 0.009 | 0.0003 | 0.0015 | 0.0054 | 0.0078 | 0.0048 | 0.0045 | 0.0001 | 0.0011 | 0.001 | 0.0508 | 0.0002 | 0.0158 | 0 | 0.0006 | 0.0009 | 0.0002 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1093.07 | 1102.9221 | 1166.1018 | 1099.2511 | 1051.3144 | 1005.3325 | 1073.7279 | 1065.2986 | 1106.2476 | 946.01 |
Translational Efficiency | 1.4655 | 1.6399 | 1.5332 | 1.7415 | 1.629 | 1.4375 | 1.4743 | 1.4998 | 1.4523 | 1.5994 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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