Standard name
Human Ortholog
Description Conserved protein of the mitochondrial matrix; performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); ISU1 has a paralog, ISU2, that arose from the whole genome duplication; isu1 isu2 double mutant is inviable; human homolog ISCU implicated in mitochondrial myopathy, can complement isu1 isu2 double mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 – 0 0 0.07 0.08 0.08 0.11 0.1
Bud 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.06 0 – 0 0 0 0.05 0 0 0 0 0.1 – 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 – 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0.08 0.1 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0.4 – 0.14 0.15 0.23 0.22 0.27 0.26 0.24
Mitochondria 0.86 0.85 0.97 0.95 0.92 0.93 – 0.93 0.94 0.93 0.88 0.92 0.97 0.87 0.95 0.08 – 0.14 0.72 0.59 0.6 0.53 0.46 0.53
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0.11 0.13 0.15 0.14 0.38 0.23 – 0.2 0.23 0.17 0.15 0.19 0.13 0.17 0.06 0.18 – 0.51 0 0 0 0 0.07 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.19 0 0 0.06 0.05 – 0 0 0 0 0 0.08 0.12 0.08 0.28 – 0.2 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 17 1 4 0 0 1 – 0 0 2 1 0 0 1 3 6 – 1 36 28 9 14 18 20
Bud 4 1 1 1 1 0 – 1 0 0 1 1 0 1 0 0 – 0 4 1 1 1 0 1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 3 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 – 0 – – – – – –
Cell Periphery 10 2 0 0 0 1 – 0 0 0 0 0 2 0 0 1 – 0 0 0 0 0 0 0
Cytoplasm 4 2 1 1 3 2 – 1 5 7 7 5 1 1 2 9 – 3 1 1 0 1 0 0
Endoplasmic Reticulum 1 0 0 0 0 0 – 0 0 1 5 2 0 1 1 5 – 2 3 1 0 2 0 1
Endosome 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 1 5 0 1 0 1 1
Golgi 68 34 1 0 0 1 – 2 1 0 3 1 3 7 7 35 – 5 137 88 26 47 41 49
Mitochondria 713 276 105 21 44 90 – 178 165 167 122 142 223 128 169 7 – 5 680 228 71 95 75 110
Nucleus 4 0 0 0 0 1 – 0 1 0 0 0 0 0 0 0 – 0 2 0 0 0 0 0
Nuclear Periphery 3 1 1 0 0 0 – 1 2 1 3 6 0 0 0 0 – 0 3 1 0 0 0 0
Nucleolus 90 41 16 3 18 22 – 39 40 31 21 30 29 25 10 16 – 18 20 11 3 4 11 9
Peroxisomes 4 0 0 0 0 1 – 1 1 0 0 0 0 0 0 1 – 1 7 5 1 5 6 3
SpindlePole 1 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 – 0 2 0 0 0 0 1
Vac/Vac Membrane 89 61 3 0 3 5 – 8 2 4 0 1 18 18 15 24 – 7 30 8 1 3 4 6
Unique Cell Count 830 325 108 22 48 97 192 176 180 138 155 230 147 178 87 35 944 385 120 180 163 209
Labelled Cell Count 1012 419 132 26 69 124 231 217 213 164 189 276 182 207 104 43 944 385 120 180 163 209


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 43.9 90.8 39.4 54.6 60.8 42.7 – 35.0 33.0 29.9 30.2 32.2 63.7 67.9 66.7 68.7 – 68.6 73.6 75.4 78.8
Std Deviation (1e-4) 14.9 29.6 14.9 23.5 25.9 20.0 – 14.2 14.8 13.4 14.2 16.7 19.7 25.1 25.0 34.2 – 35.2 28.4 32.4 34.6
Intensity Change (Log2) – – – 0.47 0.62 0.11 – -0.17 -0.26 -0.4 -0.38 -0.29 0.69 0.78 0.76 0.8 – 0.8 0.9 0.94 1.0

WT3RAP60RAP140RAP220RAP380RAP460RAP540RAP620RAP700050100WT3HU80HU120HU160050100WT3rpd3Δ_1rpd3Δ_3050100WT1AF100AF140AF180050100
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Cytoplasm 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 7.0 – 0
Mitochondria -0.4 -1.5 -1.5 – -1.6 -1.3 -1.6 -2.6 -1.9 -0.1 -2.9 -0.9 -12.5 – -10.1
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Nucleolus 0 3.2 1.4 – 1.2 1.6 0.5 0.1 1.0 -0.6 0.5 -2.6 0.7 – 4.4
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Vacuole 0 0 0 – 0 0 0 0 0 1.8 2.7 1.9 5.0 – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 186.2067 207.5609 190.8843 69.1482 202.3676 194.4317 138.3862 168.9697 152.2411 151.8637 174.9399 147.0866 166.6729 159.2805 146.8431 126.4266 159.3858 145.2527
Actin 0.0199 0.0004 0.001 0.0004 0.0016 0.0001 0.022 0.0004 0.0011 0.0002 0 0.0043 0.0002 0.0004 0.0005 0 0.0001 0
Bud 0.0001 0 0 0.0001 0.0006 0 0.0001 0 0 0 0 0.0001 0 0 0 0 0 0
Bud Neck 0.0002 0 0.0001 0 0.0001 0 0.0004 0.0002 0.0001 0 0 0 0 0 0.0002 0 0 0
Bud Periphery 0.0001 0 0.0001 0.0001 0.0015 0 0.0002 0 0 0 0 0.0002 0 0 0 0 0 0
Bud Site 0.0004 0.0001 0.0001 0.0002 0.0007 0 0.0006 0.0002 0.0002 0 0 0.0003 0.0001 0 0.0001 0 0 0
Cell Periphery 0.0001 0.0002 0.0009 0.0005 0.0002 0.0004 0.0004 0.0012 0.0008 0.0003 0 0.0003 0.0006 0.0003 0.0003 0.0001 0 0
Cytoplasm 0.0109 0 0 0.0007 0 0 0.0005 0 0.0006 0 0 0 0.0002 0 0 0 0 0
Cytoplasmic Foci 0.005 0 0.0024 0.0542 0.0005 0 0.0223 0.0001 0.003 0 0 0.0012 0.0017 0.0001 0.0002 0 0 0
Eisosomes 0.0032 0.0019 0.0124 0.0017 0.0005 0.007 0.0026 0.0043 0.0146 0.002 0.0004 0.0099 0.0039 0.0021 0.0093 0.0056 0.0049 0.0003
Endoplasmic Reticulum 0.0006 0 0.0002 0.0005 0 0 0.0004 0 0.0001 0 0 0 0 0 0 0 0 0
Endosome 0.0021 0 0.0019 0.048 0.0012 0 0.0102 0 0.0005 0 0 0.0003 0 0 0 0 0 0
Golgi 0.0018 0.0001 0.001 0.0083 0.0013 0.0001 0.0071 0.0001 0.0008 0.0002 0 0.0008 0.0001 0.0001 0.0001 0 0 0.0001
Lipid Particles 0.0144 0.0026 0.0115 0.158 0.0021 0.0008 0.0256 0.004 0.0044 0.003 0.0008 0.0101 0.0025 0.0045 0.0112 0.0004 0.0001 0.013
Mitochondria 0.9171 0.9874 0.9506 0.6085 0.981 0.9851 0.8497 0.9552 0.9379 0.9872 0.9967 0.9531 0.9659 0.964 0.9486 0.993 0.9935 0.9839
None 0.0008 0 0.0002 0.0034 0 0 0.0016 0 0.0001 0 0 0.0001 0.0012 0 0 0 0 0
Nuclear Periphery 0.0015 0 0.0015 0.0017 0 0 0.0018 0 0.0027 0 0 0.0003 0.0001 0 0 0 0 0
Nucleolus 0.0036 0.0017 0.0017 0.0793 0.0011 0.0022 0.0236 0.0202 0.0126 0.0002 0.0001 0.0054 0.0128 0.0143 0.016 0 0 0.0001
Nucleus 0.0014 0.0001 0.0002 0.0025 0.0002 0.0002 0.0012 0.0009 0.0063 0.0001 0 0.0004 0.0007 0.0005 0.0007 0 0 0
Peroxisomes 0.0139 0.0019 0.004 0.0125 0.0038 0.0004 0.0148 0.0019 0.0045 0.0003 0.0003 0.0052 0.0012 0.0017 0.0018 0.0004 0.0011 0.0003
Punctate Nuclear 0.0004 0 0.0001 0.0031 0 0 0.003 0 0.0002 0 0 0.0008 0.0001 0 0 0 0 0
Vacuole 0.0006 0.0003 0.0014 0.0015 0.0009 0.0004 0.0014 0.0028 0.002 0.0004 0.0001 0.0005 0.0008 0.0009 0.0004 0 0 0
Vacuole Periphery 0.0019 0.0031 0.0086 0.0149 0.0025 0.0032 0.0105 0.0085 0.0075 0.0059 0.0016 0.0068 0.0077 0.0108 0.0105 0.0005 0.0002 0.0022

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 89.7347 81.2077 69.0781 75.8413 73.2542 190.4423 142.6894 133.8143 177.7999 134.641
Translational Efficiency 1.8859 2.1599 1.9506 2.2749 1.9415 1.9333 1.8071 2.0248 1.6608 1.8022

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1045 1065 960 861 1460 1596 1384 1421 2505 2661 2344 2282

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2051.99 3265.20 4224.71 4874.91 3450.40 3035.80 3730.87 5435.30 2867.03 3127.61 3933.13 5223.86
Standard Deviation 644.13 1270.92 1239.58 1934.43 1151.22 1019.22 909.66 1856.15 1192.05 1132.32 1084.83 1905.53
Intensity Change Log 2 — 0.670148 1.041829 1.248352 — -0.184687 0.112748 0.655596 — 0.195522 0.531909 0.905943

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002156 0.009532 0.003100 0.010176 0.002060 0.004745 0.004433 0.004375 0.002100 0.006661 0.003887 0.006564
Bud Neck 0.001554 0.002095 0.003449 0.005101 0.000727 0.001341 0.001457 0.002370 0.001072 0.001643 0.002273 0.003400
Bud Site 0.002298 0.002899 0.001349 0.007523 0.000462 0.001201 0.000881 0.002477 0.001228 0.001880 0.001073 0.004381
Cell Periphery 0.000322 0.000177 0.000083 0.001351 0.000067 0.000179 0.000062 0.000318 0.000174 0.000178 0.000070 0.000708
Cytoplasm 0.000224 0.000536 0.000222 0.000728 0.000079 0.000576 0.000190 0.000410 0.000140 0.000560 0.000203 0.000530
Cytoplasmic Foci 0.015535 0.022068 0.039670 0.022191 0.012901 0.018549 0.020078 0.013612 0.014000 0.019957 0.028102 0.016849
Eisosomes 0.003665 0.005677 0.001823 0.004076 0.005798 0.002478 0.002511 0.002704 0.004908 0.003758 0.002229 0.003221
Endoplasmic Reticulum 0.000116 0.000126 0.000049 0.000672 0.000026 0.000061 0.000039 0.000101 0.000063 0.000087 0.000043 0.000316
Endosome 0.000427 0.000761 0.000508 0.004090 0.000079 0.000659 0.000242 0.000462 0.000224 0.000700 0.000351 0.001831
Golgi 0.007137 0.007666 0.006732 0.028701 0.002625 0.010078 0.007485 0.010416 0.004507 0.009112 0.007177 0.017315
Lipid Particles 0.005222 0.008759 0.006287 0.015454 0.003175 0.005265 0.004228 0.002502 0.004029 0.006663 0.005071 0.007389
Mitochondria 0.920181 0.900030 0.875952 0.801159 0.945910 0.926350 0.924714 0.926597 0.935177 0.915816 0.904743 0.879269
Mitotic Spindle 0.000934 0.000656 0.007610 0.001571 0.000379 0.001097 0.001561 0.000686 0.000611 0.000920 0.004039 0.001020
None 0.005631 0.004619 0.006283 0.009486 0.005917 0.004251 0.004960 0.005359 0.005798 0.004398 0.005502 0.006916
Nuclear Periphery 0.000323 0.000492 0.003104 0.001788 0.000202 0.000288 0.000546 0.000392 0.000253 0.000369 0.001594 0.000918
Nuclear Periphery Foci 0.000371 0.000542 0.000765 0.000542 0.000104 0.000138 0.000153 0.000138 0.000216 0.000300 0.000404 0.000290
Nucleolus 0.001815 0.001420 0.003623 0.002929 0.001451 0.001869 0.001512 0.001502 0.001603 0.001689 0.002376 0.002041
Nucleus 0.000229 0.000163 0.000162 0.001305 0.000059 0.000301 0.000072 0.000463 0.000130 0.000245 0.000109 0.000780
Peroxisomes 0.017645 0.020052 0.012173 0.003839 0.010807 0.010503 0.013190 0.003973 0.013659 0.014325 0.012773 0.003922
Vacuole 0.005410 0.005454 0.008694 0.022092 0.002858 0.004204 0.003014 0.007339 0.003922 0.004704 0.005340 0.012906
Vacuole Periphery 0.008806 0.006275 0.018362 0.055228 0.004315 0.005869 0.008671 0.013803 0.006189 0.006031 0.012640 0.029433

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.01 -1.64 -4.60 -0.23 -4.03 -3.51 -2.94 -3.23 0.38 0.04 -6.10 -3.37 -5.61 0.14 -3.03
Bud Neck -1.25 -3.68 -4.42 -3.80 -2.17 -2.10 -2.70 -3.20 -1.99 -1.83 -2.27 -4.51 -5.26 -3.98 -2.66
Bud Site -0.64 1.07 -3.33 -2.88 -4.45 -2.17 -1.82 -3.39 -2.13 -2.79 -1.36 0.40 -4.46 -3.49 -5.20
Cell Periphery 0.78 1.47 -2.78 -3.36 -3.67 -1.19 0.23 -2.56 -1.11 -2.64 -0.14 1.49 -3.45 -3.44 -4.43
Cytoplasm -1.89 0.09 -2.83 -0.48 -2.87 -1.66 -5.13 -3.54 0.19 -2.67 -2.32 -2.97 -4.46 -0.06 -3.90
Cytoplasmic Foci -2.56 -6.53 -2.34 -0.09 3.92 -2.60 -3.17 -0.75 1.63 2.17 -3.61 -6.94 -2.03 1.22 4.51
Eisosomes -2.83 4.37 -0.63 2.07 -4.32 5.60 5.67 5.38 -0.70 -0.65 2.54 7.02 3.98 1.50 -3.73
Endoplasmic Reticulum -0.41 2.49 -3.64 -3.50 -4.13 -4.03 -2.13 -3.22 -1.61 -2.61 -1.52 1.71 -4.23 -3.75 -4.64
Endosome -1.34 -0.89 -3.11 -2.76 -3.04 -4.41 -3.84 -2.86 1.03 -1.61 -3.69 -2.81 -3.55 -2.40 -3.27
Golgi -0.40 0.33 -6.73 -6.59 -6.99 -7.14 -4.68 -5.75 -0.20 -1.81 -5.54 -3.36 -8.58 -5.19 -6.50
Lipid Particles -1.90 -1.01 -4.03 -2.27 -3.68 -2.65 -1.40 0.92 3.75 2.47 -2.97 -1.68 -3.14 -0.59 -2.22
Mitochondria 2.59 5.06 10.68 8.49 6.35 3.66 3.85 3.79 0.26 0.03 4.28 6.25 10.14 6.32 4.45
Mitotic Spindle 0.64 -4.22 -1.08 -1.95 3.78 -1.55 -3.04 -1.57 0.82 2.19 -0.95 -4.96 -1.61 -0.36 4.33
None 2.29 -1.42 -5.77 -7.73 -5.00 4.67 2.53 1.27 -2.78 -0.95 5.04 1.02 -2.96 -7.28 -3.95
Nuclear Periphery -0.96 -4.13 -7.44 -5.05 1.89 -2.09 -6.61 -3.94 -1.92 2.34 -1.56 -4.82 -8.16 -5.19 2.34
Nuclear Periphery Foci -0.40 -1.59 -0.77 0.01 1.46 -1.68 -2.25 -1.73 -0.19 0.42 -0.49 -1.82 -0.85 0.06 1.73
Nucleolus 0.87 -3.22 -2.03 -3.61 1.18 -0.91 -0.18 -0.29 0.72 -0.11 -0.26 -2.46 -1.57 -1.25 0.96
Nucleus 0.66 0.91 -2.03 -2.12 -2.15 -2.12 -0.95 -3.02 -1.23 -2.94 -1.64 0.68 -3.06 -2.47 -3.16
Peroxisomes -0.70 1.81 5.22 7.04 4.97 0.15 -1.05 3.89 4.17 4.93 -0.36 0.48 6.43 7.86 6.77
Vacuole -0.12 -3.00 -6.87 -6.71 -5.50 -1.90 -0.27 -4.53 -3.15 -4.70 -1.32 -2.50 -8.04 -7.20 -6.91
Vacuole Periphery 1.61 -4.50 -10.50 -11.32 -8.09 -2.14 -3.65 -6.25 -5.25 -2.90 0.19 -5.72 -11.79 -12.12 -8.01
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Conserved protein of the mitochondrial matrix; performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); ISU1 has a paralog, ISU2, that arose from the whole genome duplication; isu1 isu2 double mutant is inviable; human homolog ISCU implicated in mitochondrial myopathy, can complement isu1 isu2 double mutant
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Isu1

Isu1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Isu1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available