Standard name
Human Ortholog
Description MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.96 0.99 0.93 0.78 0.74 0.73 0.65 0.58 0.5 0.43 1.0 1.0 1.0 0.97 0.98 0.94 0.85 0.91 0.85 0.92 0.91 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0 0 0 0.11 0.41 0.43 0.42 0.63 0.69 0.7 0.74 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 1 3
Bud 2 0 3 2 0 5 4 7 5 7 4 11 0 0 0 0 1 0 1 1 3 0 1 4
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 2 0 1 2
Bud Site 0 0 0 0 0 0 0 1 1 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 4 4 0 2 2 4 4 6 2 7 1 1 1 2 6 2 0 1 0 0 0 0
Cytoplasm 90 317 180 147 192 241 295 367 208 211 119 153 214 339 306 201 119 109 83 297 163 121 193 163
Endoplasmic Reticulum 0 5 3 0 1 0 1 4 0 3 2 3 1 1 0 9 3 7 0 0 1 0 1 2
Endosome 0 0 0 0 3 4 4 9 0 1 2 1 0 0 1 1 3 3 1 3 3 1 0 1
Golgi 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 1 2
Mitochondria 10 12 6 6 23 125 173 212 200 250 167 262 0 0 0 5 1 2 4 8 8 3 4 2
Nucleus 0 0 0 0 1 2 1 5 1 0 3 1 0 0 0 2 1 0 0 1 0 0 0 0
Nuclear Periphery 1 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 7 4 1 2 1 0 0 0 0 0 0 0 1 0 0 1 0
Vac/Vac Membrane 0 0 0 2 2 5 8 9 6 2 4 8 0 0 0 2 1 0 4 3 1 0 1 10
Unique Cell Count 93 328 187 148 206 308 400 503 318 361 238 354 215 340 307 207 122 116 98 327 193 133 212 201
Labelled Cell Count 103 338 196 161 223 385 492 626 430 482 306 448 216 341 308 222 135 125 98 327 193 133 212 201


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.4 4.8 4.8 4.4 3.8 3.2 3.9 3.5 3.3 3.5 3.3 5.0 4.9 5.0 5.2 6.1 6.2 4.6 4.7 5.4
Std Deviation (1e-4) 0.9 0.7 1.4 1.8 1.6 1.6 0.8 1.4 1.3 1.1 1.0 1.1 1.2 1.5 1.4 1.2 1.4 1.2 1.4 1.1 1.7
Intensity Change (Log2) – – – -0.02 -0.14 -0.34 -0.6 -0.3 -0.46 -0.54 -0.47 -0.56 0.05 0.03 0.05 0.11 0.34 0.36 -0.06 -0.05 0.16

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000246WT3HU80HU120HU1600246WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30246WT1AF100AF140AF1800246
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.437 -0.1149 -0.3949 -0.5722 -0.2163 -0.2062 0.859 0.7238 0.8721 0.7255 1.1649 0.8453 2.0406 1.1177 1.391 1.1146 2.3593 1.5452
Actin 0.0776 0.0004 0.0033 0.0003 0.0149 0.0077 0.0372 0.0008 0.015 0.0295 0.0203 0.0141 0.1309 0.0005 0.0071 0.0001 0.0158 0.0008
Bud 0.0016 0.0001 0.0001 0.0001 0.0007 0.0001 0.001 0.0019 0.0014 0.0006 0.0004 0.0005 0.002 0.0003 0.0007 0 0.0004 0.0004
Bud Neck 0.004 0.0001 0.0028 0.0001 0.002 0.0101 0.0022 0.0001 0.0006 0.0001 0.0005 0.0006 0.0016 0.0001 0.0001 0.0001 0.0012 0.0001
Bud Periphery 0.0032 0.0002 0.0002 0.0002 0.0007 0.0004 0.0022 0.0057 0.0033 0.0008 0.0008 0.0012 0.0084 0.0006 0.0033 0 0.0003 0.0009
Bud Site 0.0191 0.0007 0.0017 0.0001 0.0185 0.0002 0.0104 0.0063 0.0098 0.0003 0.0015 0.0004 0.0093 0.0019 0.0048 0 0.0036 0.0001
Cell Periphery 0.0013 0.0003 0.0003 0.0001 0.0004 0.0002 0.0005 0.0005 0.0004 0.0001 0.0001 0.0001 0.0012 0.0003 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.0566 0.0945 0.1824 0.1538 0.2646 0.2767 0.11 0.1236 0.1132 0.0877 0.1173 0.1697 0.037 0.285 0.2019 0.1908 0.0374 0.1993
Cytoplasmic Foci 0.0048 0.0038 0.0097 0.0154 0.0229 0.009 0.0313 0.0059 0.0071 0.0075 0.0168 0.0072 0.0085 0.0083 0.0089 0.001 0.0447 0.0054
Eisosomes 0.0011 0.0003 0.0015 0.0001 0.0004 0.0004 0.0009 0.0003 0.0003 0.0002 0.0003 0.0002 0.0017 0.0008 0.0003 0 0.0002 0
Endoplasmic Reticulum 0.0019 0.0008 0.0026 0.0018 0.0025 0.002 0.0075 0.0006 0.0025 0.0016 0.0022 0.0009 0.0215 0.0025 0.0013 0.0007 0.0004 0.0005
Endosome 0.0046 0.0004 0.0032 0.0024 0.0134 0.0011 0.0194 0.0032 0.0033 0.0023 0.0551 0.0051 0.0301 0.0026 0.003 0.0002 0.003 0.0008
Golgi 0.0032 0.0001 0.001 0.0002 0.005 0.0006 0.009 0.0015 0.0021 0.0024 0.0141 0.0088 0.0352 0.0004 0.0018 0 0.0056 0.0006
Lipid Particles 0.0031 0.001 0.0014 0.0009 0.0102 0.0005 0.0147 0.0055 0.0015 0.0074 0.0295 0.0049 0.0061 0.0006 0.0046 0.0001 0.0355 0.0009
Mitochondria 0.001 0.0001 0.0009 0.0003 0.0017 0.0005 0.008 0.0006 0.0009 0.0014 0.0069 0.0063 0.0259 0.0003 0.008 0.0001 0.0312 0.0012
None 0.8084 0.8954 0.7657 0.8196 0.6056 0.6883 0.7061 0.8404 0.8277 0.8292 0.7046 0.7692 0.6229 0.6921 0.7408 0.806 0.7928 0.7772
Nuclear Periphery 0.0016 0.0003 0.001 0.0006 0.0022 0.0004 0.0055 0.0002 0.0012 0.0009 0.0061 0.0004 0.0079 0.0005 0.0005 0.0002 0.0001 0.0001
Nucleolus 0.0005 0.0001 0.0032 0.0002 0.002 0.0001 0.0004 0.0002 0.0003 0.0001 0.0005 0.0001 0.0073 0.0003 0.0004 0 0.0007 0
Nucleus 0.0006 0.0003 0.0042 0.0007 0.0021 0.0004 0.0012 0.0004 0.0008 0.0005 0.0011 0.0003 0.0317 0.0006 0.0005 0.0003 0.0002 0.0001
Peroxisomes 0.0018 0.0001 0.0104 0.0002 0.0081 0.0003 0.0241 0.0015 0.0068 0.0255 0.01 0.0089 0.0034 0.0001 0.0093 0 0.0245 0.011
Punctate Nuclear 0.0025 0.0005 0.0025 0.0018 0.0205 0.0006 0.0045 0.0003 0.0008 0.0003 0.009 0.0005 0.0026 0.0012 0.0016 0.0001 0.0019 0.0003
Vacuole 0.0013 0.0004 0.0017 0.0009 0.0013 0.0004 0.0025 0.0005 0.001 0.0013 0.0016 0.0005 0.0029 0.0009 0.0006 0.0002 0.0002 0.0002
Vacuole Periphery 0.0003 0 0.0002 0.0002 0.0003 0.0001 0.0014 0.0001 0.0002 0.0004 0.0012 0.0002 0.0018 0.0001 0.0002 0 0.0003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.0681 5.8365 7.6995 7.8862 7.5246 7.2505 8.5241 8.6298 8.0854 9.7192
Translational Efficiency 0.9306 1.5639 0.8369 0.7626 1.0206 1.5661 0.9578 1.1053 0.8478 0.9607

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
127 209 145 1164 1407 1212 668 107 1534 1421 813 1271

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 706.18 713.54 948.47 853.56 711.37 738.07 998.54 911.02 710.94 734.46 989.61 858.40
Standard Deviation 65.96 87.81 106.62 115.93 79.35 100.33 100.40 149.43 78.34 98.97 103.33 120.18
Intensity Change Log 2 — 0.014958 0.425566 0.273457 — 0.053158 0.489220 0.356883 — 0.034254 0.457861 0.315925

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000059 0.000321 0.000766 0.000444 0.000112 0.000844 0.000148 0.001251 0.000108 0.000767 0.000258 0.000512
Bud Neck 0.011175 0.068065 0.002487 0.006823 0.011197 0.042133 0.002404 0.008720 0.011195 0.045948 0.002419 0.006982
Bud Site 0.004234 0.035545 0.018130 0.054043 0.004657 0.026179 0.006273 0.011308 0.004622 0.027557 0.008388 0.050445
Cell Periphery 0.000165 0.000242 0.000292 0.000150 0.000118 0.000195 0.000107 0.000249 0.000122 0.000202 0.000140 0.000158
Cytoplasm 0.557450 0.286537 0.343869 0.550978 0.561119 0.352788 0.543457 0.294354 0.560816 0.343044 0.507860 0.529374
Cytoplasmic Foci 0.237144 0.335907 0.033679 0.027938 0.222603 0.305951 0.028871 0.068342 0.223807 0.310357 0.029729 0.031339
Eisosomes 0.000209 0.000234 0.000111 0.000048 0.000150 0.000270 0.000053 0.000110 0.000155 0.000265 0.000063 0.000053
Endoplasmic Reticulum 0.000275 0.000978 0.007049 0.001469 0.000625 0.000621 0.001826 0.003178 0.000596 0.000673 0.002758 0.001613
Endosome 0.017487 0.058183 0.009511 0.006907 0.013075 0.050911 0.005824 0.064069 0.013440 0.051980 0.006482 0.011720
Golgi 0.000866 0.006397 0.000184 0.003361 0.002526 0.014526 0.000108 0.035236 0.002388 0.013331 0.000122 0.006045
Lipid Particles 0.004213 0.008866 0.003957 0.001007 0.005765 0.007363 0.001607 0.010475 0.005636 0.007584 0.002026 0.001804
Mitochondria 0.000479 0.021093 0.000407 0.001281 0.003183 0.014032 0.000185 0.022780 0.002960 0.015070 0.000224 0.003091
Mitotic Spindle 0.000016 0.001059 0.000974 0.034306 0.003724 0.009248 0.002464 0.040723 0.003417 0.008043 0.002198 0.034846
None 0.001066 0.000741 0.002080 0.004926 0.002743 0.001371 0.002175 0.004335 0.002604 0.001278 0.002158 0.004877
Nuclear Periphery 0.000199 0.000207 0.001757 0.000951 0.000319 0.000789 0.000956 0.002242 0.000309 0.000703 0.001099 0.001060
Nuclear Periphery Foci 0.000271 0.000498 0.006156 0.001222 0.000435 0.000521 0.003468 0.011011 0.000422 0.000518 0.003947 0.002046
Nucleolus 0.000492 0.001093 0.000587 0.000256 0.000723 0.001059 0.000245 0.000321 0.000704 0.001064 0.000306 0.000261
Nucleus 0.074262 0.046619 0.370529 0.119920 0.100883 0.044174 0.153944 0.209895 0.098679 0.044533 0.192572 0.127495
Peroxisomes 0.001831 0.018951 0.000782 0.000639 0.003427 0.029020 0.000199 0.013486 0.003295 0.027539 0.000303 0.001720
Vacuole 0.087234 0.104848 0.196406 0.181884 0.061626 0.094178 0.245231 0.187615 0.063746 0.095748 0.236523 0.182366
Vacuole Periphery 0.000872 0.003617 0.000288 0.001446 0.000988 0.003828 0.000458 0.010299 0.000979 0.003797 0.000428 0.002192

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.52 -4.62 -7.74 -0.10 2.92 -6.01 -2.69 -2.88 0.57 -2.63 -6.30 -4.93 -6.59 3.69 -1.68
Bud Neck -5.68 5.15 3.66 6.40 -3.60 -10.53 8.42 2.61 10.65 -1.51 -11.88 9.15 6.00 14.54 -4.22
Bud Site -4.83 -1.20 -5.83 1.82 -1.43 -10.16 -0.19 -1.41 7.13 -1.30 -11.17 -1.05 -5.97 1.88 -5.00
Cell Periphery -2.05 -4.08 3.09 4.07 7.28 -6.01 3.59 -2.75 0.56 -3.78 -6.60 -0.85 1.16 6.56 1.67
Cytoplasm 10.76 9.89 6.59 -7.16 -6.11 19.89 8.84 10.42 2.40 5.85 22.47 12.01 14.49 -6.14 0.95
Cytoplasmic Foci -4.37 13.70 14.69 18.59 2.97 -9.89 37.58 19.31 25.93 -3.10 -10.94 39.68 40.11 46.11 1.19
Eisosomes -0.77 3.85 7.43 7.40 6.96 -6.48 11.64 2.30 7.42 -5.30 -6.67 11.21 12.50 13.44 2.10
Endoplasmic Reticulum -2.10 -9.25 -13.71 -3.46 6.97 0.03 -11.47 -3.59 -3.59 -1.10 -0.65 -13.09 -11.21 -10.21 4.81
Endosome -5.38 2.49 4.62 8.05 5.53 -13.55 8.44 -1.83 2.44 -2.83 -14.71 8.23 6.45 17.95 0.13
Golgi -4.31 3.44 -0.28 3.96 -1.72 -7.79 5.03 -1.94 -0.09 -2.33 -8.16 5.13 0.26 7.72 -2.98
Lipid Particles -2.76 0.01 4.60 4.93 7.44 -2.09 9.03 0.01 0.50 -1.30 -2.74 8.29 8.22 9.46 1.70
Mitochondria -2.91 0.53 -1.10 2.88 -2.15 -5.30 3.41 -0.87 0.54 -1.28 -5.89 3.39 1.47 6.85 -1.94
Mitotic Spindle -3.91 -2.48 -6.47 -6.04 -6.02 -2.48 0.73 -1.55 -0.95 -1.69 -2.36 0.80 -4.85 -3.06 -5.60
None 0.76 -1.63 -4.74 -6.45 -3.36 1.79 1.31 -0.19 -1.18 -0.78 1.95 1.17 -2.02 -4.11 -4.03
Nuclear Periphery -0.13 -13.10 -20.38 -19.71 1.24 -0.85 -8.22 -2.87 -1.44 -0.95 -0.83 -10.10 -12.10 -2.47 -3.57
Nuclear Periphery Foci -2.06 -7.54 -12.45 -6.46 6.19 -0.43 -12.82 -1.18 -1.16 -0.37 -0.59 -14.49 -3.82 -3.31 5.79
Nucleolus -2.50 -0.26 1.42 4.38 2.08 -2.21 6.71 4.31 5.24 -1.19 -2.65 5.85 5.82 6.71 -0.53
Nucleus 2.86 -11.28 -11.04 -16.25 7.21 15.19 -8.92 -6.42 -8.89 -3.43 15.49 -12.81 -13.78 -23.36 1.82
Peroxisomes -5.94 3.14 4.87 6.50 1.56 -11.63 7.50 -0.84 6.31 -1.96 -12.55 7.57 5.28 14.05 -2.14
Vacuole -1.36 -7.90 -17.35 -16.67 -2.11 -7.68 -24.23 -9.21 -7.85 1.20 -7.99 -25.74 -30.37 -25.16 0.32
Vacuole Periphery -3.86 2.01 1.14 4.70 -1.25 -5.07 3.71 -1.35 -0.25 -1.64 -5.73 4.00 0.51 5.58 -2.03
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (62%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mkk2

Mkk2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mkk2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available