Standard name | |
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Human Ortholog | |
Description | MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 3 |
Bud | 2 | 0 | 3 | 2 | 0 | 5 | 4 | 7 | 5 | 7 | 4 | 11 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 0 | 1 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 4 | 4 | 0 | 2 | 2 | 4 | 4 | 6 | 2 | 7 | 1 | 1 | 1 | 2 | 6 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 90 | 317 | 180 | 147 | 192 | 241 | 295 | 367 | 208 | 211 | 119 | 153 | 214 | 339 | 306 | 201 | 119 | 109 | 83 | 297 | 163 | 121 | 193 | 163 |
Endoplasmic Reticulum | 0 | 5 | 3 | 0 | 1 | 0 | 1 | 4 | 0 | 3 | 2 | 3 | 1 | 1 | 0 | 9 | 3 | 7 | 0 | 0 | 1 | 0 | 1 | 2 |
Endosome | 0 | 0 | 0 | 0 | 3 | 4 | 4 | 9 | 0 | 1 | 2 | 1 | 0 | 0 | 1 | 1 | 3 | 3 | 1 | 3 | 3 | 1 | 0 | 1 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 2 |
Mitochondria | 10 | 12 | 6 | 6 | 23 | 125 | 173 | 212 | 200 | 250 | 167 | 262 | 0 | 0 | 0 | 5 | 1 | 2 | 4 | 8 | 8 | 3 | 4 | 2 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 5 | 1 | 0 | 3 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 7 | 4 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 2 | 2 | 5 | 8 | 9 | 6 | 2 | 4 | 8 | 0 | 0 | 0 | 2 | 1 | 0 | 4 | 3 | 1 | 0 | 1 | 10 |
Unique Cell Count | 93 | 328 | 187 | 148 | 206 | 308 | 400 | 503 | 318 | 361 | 238 | 354 | 215 | 340 | 307 | 207 | 122 | 116 | 98 | 327 | 193 | 133 | 212 | 201 |
Labelled Cell Count | 103 | 338 | 196 | 161 | 223 | 385 | 492 | 626 | 430 | 482 | 306 | 448 | 216 | 341 | 308 | 222 | 135 | 125 | 98 | 327 | 193 | 133 | 212 | 201 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 4.4 | 4.8 | 4.8 | 4.4 | 3.8 | 3.2 | 3.9 | 3.5 | 3.3 | 3.5 | 3.3 | 5.0 | 4.9 | 5.0 | 5.2 | 6.1 | 6.2 | 4.6 | 4.7 | 5.4 |
Std Deviation (1e-4) | 0.9 | 0.7 | 1.4 | 1.8 | 1.6 | 1.6 | 0.8 | 1.4 | 1.3 | 1.1 | 1.0 | 1.1 | 1.2 | 1.5 | 1.4 | 1.2 | 1.4 | 1.2 | 1.4 | 1.1 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.02 | -0.14 | -0.34 | -0.6 | -0.3 | -0.46 | -0.54 | -0.47 | -0.56 | 0.05 | 0.03 | 0.05 | 0.11 | 0.34 | 0.36 | -0.06 | -0.05 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.437 | -0.1149 | -0.3949 | -0.5722 | -0.2163 | -0.2062 | 0.859 | 0.7238 | 0.8721 | 0.7255 | 1.1649 | 0.8453 | 2.0406 | 1.1177 | 1.391 | 1.1146 | 2.3593 | 1.5452 |
Actin | 0.0776 | 0.0004 | 0.0033 | 0.0003 | 0.0149 | 0.0077 | 0.0372 | 0.0008 | 0.015 | 0.0295 | 0.0203 | 0.0141 | 0.1309 | 0.0005 | 0.0071 | 0.0001 | 0.0158 | 0.0008 |
Bud | 0.0016 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.001 | 0.0019 | 0.0014 | 0.0006 | 0.0004 | 0.0005 | 0.002 | 0.0003 | 0.0007 | 0 | 0.0004 | 0.0004 |
Bud Neck | 0.004 | 0.0001 | 0.0028 | 0.0001 | 0.002 | 0.0101 | 0.0022 | 0.0001 | 0.0006 | 0.0001 | 0.0005 | 0.0006 | 0.0016 | 0.0001 | 0.0001 | 0.0001 | 0.0012 | 0.0001 |
Bud Periphery | 0.0032 | 0.0002 | 0.0002 | 0.0002 | 0.0007 | 0.0004 | 0.0022 | 0.0057 | 0.0033 | 0.0008 | 0.0008 | 0.0012 | 0.0084 | 0.0006 | 0.0033 | 0 | 0.0003 | 0.0009 |
Bud Site | 0.0191 | 0.0007 | 0.0017 | 0.0001 | 0.0185 | 0.0002 | 0.0104 | 0.0063 | 0.0098 | 0.0003 | 0.0015 | 0.0004 | 0.0093 | 0.0019 | 0.0048 | 0 | 0.0036 | 0.0001 |
Cell Periphery | 0.0013 | 0.0003 | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0005 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0012 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0566 | 0.0945 | 0.1824 | 0.1538 | 0.2646 | 0.2767 | 0.11 | 0.1236 | 0.1132 | 0.0877 | 0.1173 | 0.1697 | 0.037 | 0.285 | 0.2019 | 0.1908 | 0.0374 | 0.1993 |
Cytoplasmic Foci | 0.0048 | 0.0038 | 0.0097 | 0.0154 | 0.0229 | 0.009 | 0.0313 | 0.0059 | 0.0071 | 0.0075 | 0.0168 | 0.0072 | 0.0085 | 0.0083 | 0.0089 | 0.001 | 0.0447 | 0.0054 |
Eisosomes | 0.0011 | 0.0003 | 0.0015 | 0.0001 | 0.0004 | 0.0004 | 0.0009 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0017 | 0.0008 | 0.0003 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0019 | 0.0008 | 0.0026 | 0.0018 | 0.0025 | 0.002 | 0.0075 | 0.0006 | 0.0025 | 0.0016 | 0.0022 | 0.0009 | 0.0215 | 0.0025 | 0.0013 | 0.0007 | 0.0004 | 0.0005 |
Endosome | 0.0046 | 0.0004 | 0.0032 | 0.0024 | 0.0134 | 0.0011 | 0.0194 | 0.0032 | 0.0033 | 0.0023 | 0.0551 | 0.0051 | 0.0301 | 0.0026 | 0.003 | 0.0002 | 0.003 | 0.0008 |
Golgi | 0.0032 | 0.0001 | 0.001 | 0.0002 | 0.005 | 0.0006 | 0.009 | 0.0015 | 0.0021 | 0.0024 | 0.0141 | 0.0088 | 0.0352 | 0.0004 | 0.0018 | 0 | 0.0056 | 0.0006 |
Lipid Particles | 0.0031 | 0.001 | 0.0014 | 0.0009 | 0.0102 | 0.0005 | 0.0147 | 0.0055 | 0.0015 | 0.0074 | 0.0295 | 0.0049 | 0.0061 | 0.0006 | 0.0046 | 0.0001 | 0.0355 | 0.0009 |
Mitochondria | 0.001 | 0.0001 | 0.0009 | 0.0003 | 0.0017 | 0.0005 | 0.008 | 0.0006 | 0.0009 | 0.0014 | 0.0069 | 0.0063 | 0.0259 | 0.0003 | 0.008 | 0.0001 | 0.0312 | 0.0012 |
None | 0.8084 | 0.8954 | 0.7657 | 0.8196 | 0.6056 | 0.6883 | 0.7061 | 0.8404 | 0.8277 | 0.8292 | 0.7046 | 0.7692 | 0.6229 | 0.6921 | 0.7408 | 0.806 | 0.7928 | 0.7772 |
Nuclear Periphery | 0.0016 | 0.0003 | 0.001 | 0.0006 | 0.0022 | 0.0004 | 0.0055 | 0.0002 | 0.0012 | 0.0009 | 0.0061 | 0.0004 | 0.0079 | 0.0005 | 0.0005 | 0.0002 | 0.0001 | 0.0001 |
Nucleolus | 0.0005 | 0.0001 | 0.0032 | 0.0002 | 0.002 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0073 | 0.0003 | 0.0004 | 0 | 0.0007 | 0 |
Nucleus | 0.0006 | 0.0003 | 0.0042 | 0.0007 | 0.0021 | 0.0004 | 0.0012 | 0.0004 | 0.0008 | 0.0005 | 0.0011 | 0.0003 | 0.0317 | 0.0006 | 0.0005 | 0.0003 | 0.0002 | 0.0001 |
Peroxisomes | 0.0018 | 0.0001 | 0.0104 | 0.0002 | 0.0081 | 0.0003 | 0.0241 | 0.0015 | 0.0068 | 0.0255 | 0.01 | 0.0089 | 0.0034 | 0.0001 | 0.0093 | 0 | 0.0245 | 0.011 |
Punctate Nuclear | 0.0025 | 0.0005 | 0.0025 | 0.0018 | 0.0205 | 0.0006 | 0.0045 | 0.0003 | 0.0008 | 0.0003 | 0.009 | 0.0005 | 0.0026 | 0.0012 | 0.0016 | 0.0001 | 0.0019 | 0.0003 |
Vacuole | 0.0013 | 0.0004 | 0.0017 | 0.0009 | 0.0013 | 0.0004 | 0.0025 | 0.0005 | 0.001 | 0.0013 | 0.0016 | 0.0005 | 0.0029 | 0.0009 | 0.0006 | 0.0002 | 0.0002 | 0.0002 |
Vacuole Periphery | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0014 | 0.0001 | 0.0002 | 0.0004 | 0.0012 | 0.0002 | 0.0018 | 0.0001 | 0.0002 | 0 | 0.0003 | 0 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.0681 | 5.8365 | 7.6995 | 7.8862 | 7.5246 | 7.2505 | 8.5241 | 8.6298 | 8.0854 | 9.7192 |
Translational Efficiency | 0.9306 | 1.5639 | 0.8369 | 0.7626 | 1.0206 | 1.5661 | 0.9578 | 1.1053 | 0.8478 | 0.9607 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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