Standard name
Human Ortholog
Description Cytosolic leucyl tRNA synthetase; ligates leucine to the appropriate tRNA; human homolog LARS can complement yeast temperature-sensitive mutant at restrictive temperature

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.06 0 0.07 0.08 0.09 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.97 1.0 0.97 0.93 0.88 0.82 0.78 0.81 0.75 0.75 0.99 0.99 0.98 0.97 0.99 0.99 0.97 0.98 0.94 0.92 0.93 0.91
Endoplasmic Reticulum 0 0 0 0 0 0.06 0.09 0.13 0.14 0.12 0.18 0.14 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 4 8 12 14 8 14 27 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 0 0 3 2 3 1 6 0 0 1 0 0 0 1 0 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 13 3 14 17 29 21 32 26 28 53 0 1 5 4 2 1 0 0 1 0 0 0
Cytoplasm 149 98 349 263 348 365 439 389 338 270 245 339 123 221 247 215 176 178 143 98 345 73 184 157
Endoplasmic Reticulum 1 2 5 1 9 23 47 62 60 41 58 64 1 2 3 9 11 6 0 0 8 0 4 7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 1 0 4 0 2 4 3 0 0 0 2 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 0 2 14 11 14 27 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 2 0 0 0 0 1 2 1 3 5 0 1 0 3 0 2 0 0 4 0 0 0
Unique Cell Count 150 100 359 264 357 393 501 475 431 333 326 453 124 223 251 221 178 180 148 101 367 80 199 173
Labelled Cell Count 150 101 370 267 371 411 523 495 462 362 367 525 124 225 256 233 190 187 148 101 367 80 199 173


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 109.1 97.1 64.0 63.7 56.5 45.3 41.0 37.2 32.8 31.0 29.3 28.4 97.3 92.5 81.6 123.7 135.3 136.4 99.3 108.8 112.1
Std Deviation (1e-4) 28.8 21.4 14.7 14.1 10.9 9.9 9.4 8.3 8.5 8.1 7.3 6.8 25.3 18.1 15.0 40.3 43.7 46.0 33.2 35.5 35.2
Intensity Change (Log2) -0.01 -0.18 -0.5 -0.64 -0.78 -0.97 -1.04 -1.13 -1.17 0.6 0.53 0.35 0.95 1.08 1.09 0.63 0.77 0.81


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 3.0 3.4 0 4.0 4.7 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.9 0.2 0.5 1.5 0.6 2.3 2.4 2.7 4.2 0 -2.4 -1.2 -1.3 0 0
Cytoplasm 2.3 0.2 -2.7 -5.0 -6.9 -7.8 -6.9 -8.5 -8.8 1.3 1.6 1.0 0.1 1.2 1.2
Endoplasmic Reticulum 0 1.1 3.2 4.8 6.1 6.4 5.8 7.4 6.5 0 0 0 2.0 3.1 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.4 3.5 4.0 4.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 85.0979 137.8913 116.7705 123.9341 109.8733 115.5246 92.6923 138.3405 120.4337 98.8657 115.8812 114.3651 100.4902 143.9924 123.2225 111.1639 116.5823 119.4024
Actin 0.0461 0.0007 0.0037 0.0002 0.0005 0.0334 0.0094 0.0004 0.0054 0.006 0.0007 0.0067 0.0024 0.0002 0.0087 0.0011 0.001 0.0009
Bud 0.0035 0.0011 0.0028 0.0018 0.0011 0.0015 0.0021 0.0004 0.0035 0.0031 0.0013 0.0008 0.002 0.0002 0.0012 0.0016 0.0011 0.0046
Bud Neck 0.0018 0.0011 0.0025 0.0008 0.0027 0.0034 0.0004 0.0002 0.0005 0.0012 0.0007 0.0011 0.0008 0.0004 0.0014 0.0008 0.0012 0.0033
Bud Periphery 0.0032 0.0008 0.0039 0.0005 0.0007 0.0018 0.0027 0.0002 0.0037 0.0066 0.0007 0.0009 0.0019 0.0001 0.0008 0.0015 0.001 0.0036
Bud Site 0.0048 0.0011 0.0025 0.0002 0.0002 0.0008 0.0025 0.0002 0.003 0.0065 0.0005 0.0004 0.0021 0.0002 0.0019 0.0005 0.001 0.0004
Cell Periphery 0.0287 0.03 0.0443 0.0037 0.0198 0.0043 0.0091 0.0032 0.0099 0.0319 0.0023 0.0003 0.0152 0.0064 0.0122 0.0367 0.0069 0.0075
Cytoplasm 0.4307 0.7996 0.6322 0.6085 0.5872 0.5834 0.7813 0.9769 0.8717 0.7568 0.8805 0.8976 0.7709 0.9582 0.8621 0.7141 0.8058 0.765
Cytoplasmic Foci 0.0326 0.0005 0.01 0.0008 0.048 0.0041 0.0127 0.0008 0.0097 0.003 0.0101 0.0111 0.0099 0.0006 0.0064 0.0026 0.0047 0.0229
Eisosomes 0.0008 0.0001 0.0004 0 0.0003 0.0002 0.0002 0 0.0001 0.0002 0.0003 0 0.0004 0 0.0002 0.0003 0.0004 0.0001
Endoplasmic Reticulum 0.0533 0.0187 0.02 0.0021 0.0109 0.0763 0.0375 0.0026 0.0107 0.0102 0.0027 0.0084 0.039 0.0114 0.0116 0.0088 0.0122 0.0303
Endosome 0.0149 0.0012 0.0062 0.0041 0.007 0.0189 0.0146 0.0009 0.0046 0.0224 0.0041 0.0379 0.0103 0.0006 0.0064 0.0174 0.0098 0.0103
Golgi 0.0071 0.0002 0.0038 0.0002 0.0004 0.005 0.0038 0.0002 0.0011 0.0029 0.0006 0.0074 0.0016 0.0001 0.0013 0.0012 0.0018 0.0026
Lipid Particles 0.0169 0.0007 0.0123 0.0003 0.0105 0.0064 0.0035 0.0004 0.0024 0.0008 0.0059 0.0008 0.0028 0.0008 0.0013 0.0013 0.0015 0.0032
Mitochondria 0.0292 0.0075 0.0131 0.0005 0.0222 0.0104 0.0061 0.0001 0.0039 0.0224 0.0096 0.0029 0.0126 0 0.0106 0.0142 0.0302 0.0337
None 0.0171 0.0002 0.0043 0.0002 0.0045 0.0017 0.0085 0.0001 0.0035 0.0003 0.015 0.0012 0.0049 0.0001 0.0028 0.0022 0.0017 0.0056
Nuclear Periphery 0.0515 0.0072 0.0203 0.0163 0.014 0.0162 0.0225 0.0012 0.0023 0.008 0.0051 0.0033 0.02 0.0013 0.0072 0.0167 0.0047 0.0075
Nucleolus 0.0203 0.0002 0.0043 0.0005 0.0052 0.0104 0.0023 0.0001 0.0008 0.0003 0.0112 0.0001 0.003 0 0.0037 0.0009 0.0032 0.0056
Nucleus 0.0161 0.0033 0.0144 0.0058 0.0071 0.0351 0.0066 0.001 0.0023 0.0026 0.0066 0.0014 0.0099 0.0009 0.0042 0.0073 0.0048 0.006
Peroxisomes 0.0123 0 0.0039 0.0001 0.0029 0.0044 0.0004 0 0.0003 0.0002 0.0003 0.0014 0.0003 0 0.0003 0.0001 0.0002 0.0006
Punctate Nuclear 0.0144 0 0.0011 0 0.0004 0.0018 0.0046 0 0.0013 0.0001 0.0006 0.0017 0.0014 0 0.0003 0.0002 0.0002 0.0002
Vacuole 0.1548 0.1141 0.1646 0.2366 0.2139 0.1053 0.0607 0.0103 0.0498 0.0882 0.0234 0.0102 0.0741 0.0176 0.0494 0.1401 0.0893 0.0684
Vacuole Periphery 0.0398 0.0116 0.0293 0.1171 0.0405 0.0752 0.0086 0.0008 0.0095 0.0264 0.0178 0.0044 0.0144 0.0008 0.0058 0.0306 0.0173 0.0178

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 293.6344 282.3814 298.9903 321.2449 299.4101 263.8214 266.7758 252.7566 244.4966 284.315
Translational Efficiency 1.1557 1.231 1.1525 1.138 1.1582 1.0841 1.0717 0.9707 0.9789 0.9924

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1886 969 1298 1188 1897 1369 176 178 3783 2338 1474 1366

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 5256.95 6482.36 10331.60 6944.79 5173.33 6932.61 9047.41 7016.09 5215.02 6746.00 10178.26 6954.08
Standard Deviation 1127.94 1539.09 1740.12 1562.56 1287.10 1623.46 1291.64 1517.52 1211.10 1604.44 1743.29 1556.95
Intensity Change Log 2 0.302293 0.974766 0.401705 0.422305 0.806412 0.439574 0.363066 0.893717 0.420612

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000031 0.000221 0.000125 0.000403 0.000023 0.000132 0.000221 0.000244 0.000027 0.000169 0.000136 0.000383
Bud Neck 0.000089 0.000615 0.002635 0.001594 0.000135 0.000574 0.005848 0.002625 0.000112 0.000591 0.003018 0.001729
Bud Site 0.001507 0.003253 0.002414 0.010398 0.001214 0.003782 0.004609 0.013974 0.001360 0.003563 0.002676 0.010864
Cell Periphery 0.005319 0.013004 0.024605 0.045489 0.006293 0.018730 0.044386 0.085516 0.005808 0.016357 0.026967 0.050705
Cytoplasm 0.925841* 0.817742* 0.645126* 0.658502* 0.885055* 0.832667* 0.363180* 0.478230* 0.905388* 0.826481* 0.611461* 0.635011*
Cytoplasmic Foci 0.002152 0.006256 0.013803 0.004748 0.002653 0.007429 0.019898 0.007903 0.002403 0.006943 0.014531 0.005159
Eisosomes 0.000000 0.000003 0.000005 0.000005 0.000001 0.000002 0.000010 0.000007 0.000001 0.000003 0.000006 0.000006
Endoplasmic Reticulum 0.000177 0.000389 0.000486 0.000297 0.000310 0.000349 0.000150 0.000215 0.000243 0.000366 0.000446 0.000286
Endosome 0.000134 0.000349 0.000507 0.000780 0.000171 0.000339 0.000643 0.002381 0.000153 0.000343 0.000523 0.000988
Golgi 0.013992 0.032777 0.036593 0.010378 0.018602 0.027920 0.031440 0.012610 0.016304 0.029933 0.035978 0.010669
Lipid Particles 0.003664 0.010642 0.016214 0.026083 0.004090 0.012858 0.031910 0.042732 0.003877 0.011940 0.018088 0.028253
Mitochondria 0.000086 0.000188 0.000197 0.000559 0.000108 0.000353 0.000109 0.000105 0.000097 0.000284 0.000187 0.000499
Mitotic Spindle 0.000193 0.000816 0.002095 0.001635 0.000160 0.001074 0.001562 0.003059 0.000176 0.000967 0.002031 0.001821
None 0.003127 0.003871 0.001942 0.001832 0.002722 0.002854 0.000866 0.000872 0.002924 0.003275 0.001814 0.001707
Nuclear Periphery 0.000124 0.000521 0.002515 0.000348 0.000241 0.000405 0.003403 0.000530 0.000183 0.000453 0.002621 0.000372
Nuclear Periphery Foci 0.003217 0.010440 0.014705 0.003901 0.006606 0.006798 0.016079 0.005012 0.004916 0.008307 0.014869 0.004046
Nucleolus 0.000029 0.000337 0.000113 0.000198 0.000057 0.000110 0.000159 0.000161 0.000043 0.000204 0.000119 0.000193
Nucleus 0.009317 0.015636 0.027019 0.011225 0.006824 0.012302 0.035789 0.012582 0.008067 0.013684 0.028066 0.011402
Peroxisomes 0.000010 0.000032 0.000012 0.000092 0.000034 0.000035 0.000023 0.000143 0.000022 0.000034 0.000013 0.000099
Vacuole 0.015926 0.053243 0.150031 0.201742* 0.034044 0.050275 0.396945* 0.313241* 0.025011 0.051505 0.179513 0.216271*
Vacuole Periphery 0.015065 0.029666 0.058856 0.019790 0.030659 0.021013 0.042770 0.017857 0.022885 0.024599 0.056936 0.019538

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.43 -12.40 -2.13 -1.08 -1.63 -7.65 -6.85 -4.63 -2.29 0.05 -2.52 -14.99 -2.29 -1.50 -1.63
Bud Neck -2.72 -11.41 -11.86 -4.15 4.94 -4.46 -7.71 -3.45 -2.73 4.00 -4.84 -13.40 -11.44 -6.47 6.11
Bud Site -2.74 -3.47 -7.52 -5.47 -6.76 -4.42 -2.65 -4.10 -3.15 -1.89 -5.13 -5.01 -8.60 -6.27 -7.02
Cell Periphery -7.01 -13.85 -14.85 -11.09 -6.25 -8.66 -7.84 -8.49 -6.95 -4.18 -11.08 -15.63 -16.85 -11.89 -7.47
Cytoplasm 12.03 29.47 26.94 12.99 -2.34 6.72 21.63 15.43 13.08 -3.21 13.38 32.44 28.93 18.51 -3.06
Cytoplasmic Foci -6.39 -10.96 -4.82 1.19 7.80 -8.01 -7.85 -3.08 -0.25 5.16 -10.33 -12.65 -5.43 1.92 8.97
Eisosomes -1.71 -10.84 -9.37 -1.35 0.67 -6.18 -8.09 -7.74 -5.27 2.36 -3.25 -12.60 -10.72 -3.48 1.24
Endoplasmic Reticulum -2.87 -3.18 -2.76 1.19 1.75 -0.74 4.14 1.78 2.41 -1.35 -2.81 -2.27 -1.09 1.66 1.66
Endosome -5.69 -11.93 -3.31 -2.07 -1.14 -5.69 -7.42 -1.53 -1.39 -1.11 -8.10 -14.02 -3.34 -2.47 -1.59
Golgi -8.04 -12.63 2.99 10.07 15.76 -4.92 -3.44 3.45 7.06 5.35 -9.29 -12.76 5.59 13.20 16.60
Lipid Particles -6.03 -12.72 -13.89 -7.93 -4.48 -8.57 -10.43 -7.79 -5.81 -1.74 -10.53 -15.53 -15.89 -9.55 -4.86
Mitochondria -2.14 -7.20 -3.11 -2.35 -2.15 -4.09 -1.04 -0.49 2.01 -0.30 -4.66 -7.03 -3.09 -1.57 -2.16
Mitotic Spindle -1.82 -8.00 -2.01 -0.73 0.81 -2.86 -7.03 -1.20 -0.78 -0.55 -3.30 -8.75 -2.35 -1.01 0.53
None -1.28 4.93 2.56 2.90 0.30 -0.30 6.90 4.85 3.88 -0.33 -0.96 5.91 2.78 3.02 0.30
Nuclear Periphery -8.28 -14.90 -8.54 3.35 13.61 -4.47 -8.49 -4.02 -1.33 7.66 -9.17 -16.38 -7.69 2.55 15.17
Nuclear Periphery Foci -8.37 -13.21 -2.21 7.65 12.58 -0.42 -5.62 2.24 2.76 6.65 -6.46 -12.31 2.33 8.79 13.80
Nucleolus -1.56 -12.17 -6.50 0.67 -2.55 -1.66 -6.66 -3.87 -1.04 0.64 -1.94 -11.45 -6.51 0.11 -2.43
Nucleus -5.10 -12.13 -3.83 1.26 9.17 -4.80 -7.28 -2.86 -0.47 5.18 -6.62 -14.55 -5.62 0.30 10.38
Peroxisomes -1.38 -1.94 -9.27 -2.70 -8.94 -0.07 0.37 -2.13 -2.20 -2.36 -1.17 0.96 -6.18 -4.75 -8.54
Vacuole -9.48 -24.21 -27.66 -19.81 -4.95 -4.64 -16.81 -13.28 -12.39 2.23 -10.09 -26.52 -29.27 -24.08 -3.61
Vacuole Periphery -5.72 -14.37 -1.84 4.21 12.87 4.09 -1.23 4.60 1.61 4.03 -1.02 -12.03 2.65 3.53 13.45
Standard name
Human Ortholog
Description Cytosolic leucyl tRNA synthetase; ligates leucine to the appropriate tRNA; human homolog LARS can complement yeast temperature-sensitive mutant at restrictive temperature
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Cdc60

Cdc60


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc60-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available